CN100356851C - Fructus cnidii thuricin complex insecticide - Google Patents
Fructus cnidii thuricin complex insecticide Download PDFInfo
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- CN100356851C CN100356851C CNB2005101146849A CN200510114684A CN100356851C CN 100356851 C CN100356851 C CN 100356851C CN B2005101146849 A CNB2005101146849 A CN B2005101146849A CN 200510114684 A CN200510114684 A CN 200510114684A CN 100356851 C CN100356851 C CN 100356851C
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Abstract
The present invention discloses an agricultural application for a compound biological pesticide of osthole and bacillus thuringiensis, particularly application research for controlling lepidoptera injurious insects. The present invention provides that the range of the compound ratio of the osthole (A) to the bacillus thuringiensis (B) is from 1: 1 to 1: 60, and perfectly is from 1: 1 to 1: 25, and has synergistic effect on lepidoptera insects, such as diamondback moths and cabbage caterpillars after the osthole and the bacillus thuringiensis are compounded. The present invention is particularly suitable for controlling each instar diamondback moths and cabbage caterpillar larvas and killing nocturnal moth egg mass of cabbages, cauliflowers, green cauliflowers, Chinese cabbages, small green vegetables, etc.
Description
Technical field
The present invention relates to Osthole and bacillus thuringiensis,Bt is the biopesticide compounded pesticides of active component, and this compounded pesticides can be used for preventing and treating various pests such as Lepidoptera diamond-back moth, cabbage caterpillar, low age noctuid, noctuid pieces of an egg.
Background technology
Osthole (Osthol) chemical name is 8-(3-methyl-2-butene base) methyl ether umbelliferone, belong to coumarin kind compound, it is main component in the Carrot family plant frutus cnidii (Cnidium monnicir (L.) Cusson), also be root and the rhizome of Imperatoria ostruthium, circle Radix Angelicae Sinensis, Angelica pubescens, bolt wing celery, the root skin of rutaceae anise, Calusena lansium, the active component of the acrial part of single leaf rue etc.Pharmaceutically, effect such as that Osthole has is anticancer, anti-arrhythmia, spasmolysis are relievingd asthma.On agricultural, there has been the report Osthole to have the killing pests and suppressing bacteria biologically active, but itself and other biological agriculture chemical compounding have been had synergistic effect and the still rare report of compound proportion.
Microorganism insecticide bacillus thuringiensis,Bt (Bacillusthuringiensis, be called for short B.t) because having the crystalline protein toxin (Insecticidal Crystal Protein is called for short ICP) of strong insecticidal action, generation becomes the important biomolecule preparation of preventing and treating insects such as diamond-back moth in recent years.Yet be subjected to the restriction of biological property, problem such as the ubiquity virulence is strong in actual use, insecticidal spectrum is narrow, the longevity of residure is short, effect is slow influences large scale application.Report is arranged, and long-term a large amount of B.t that use have made various pests that bacillus thuringiensis,Bt is produced significant pesticide resistance.As cigarette beetle the false unicellular bacillus that changes the B.t killing gene has been produced 20 times of pesticide resistance, pure some product of B.t insecticide has been produced 6 times of pesticide resistance.Studies show that this mainly is because B.t is single to the mechanism of action of insect.
In order to overcome the pesticide resistance of insect to B.t, simultaneously also in order to bring into play the insecticidal effect of Osthole better, the present invention carries out botanical biological pesticide Osthole and microbial source biopesticide B.t composite, finds that it has obvious synergistic effect.From mechanism of action, Osthole is insect to be had neurovirulent action of contace poison also have stomach poison function simultaneously, is quick-acting desinsections; And B.t plays a kind of insecticidal action slowly by destroying the insect digestive tract.Therefore, from the complementation of insecticidal action mechanism, with single agent under the situation of suitable insecticidal effect, Osthole and B.t be composite to have following advantage and meaning:
1. reduce the consumption of B.t, thereby reduce the selection pressure of insect, in the hope of overcoming the pesticide resistance of insect to B.t to it;
1, reduces the consumption of Osthole, to reduce consumption to the cnidium monnieri plant;
2, slow-acting type and fast-acting type are composite, have synergistic effect from the desinsection timeliness.
Summary of the invention
The present invention is by agent of Osthole list and the composite research as agricultural insecticide of B.t, especially finishes at the control application study of lepidoptera pest and aphid.Its purpose is to provide Osthole and the B.t compound biotic insecticide that is exclusively used in control crop lepidoptera pest and aphid.
Therefore, the invention provides a kind of fructus cnidii thuricin complex insecticide, wherein the composite mass ratio range of Osthole and bacillus thuringiensis,Bt is 1: 1～60, more preferably 1: 1～25, and both composite backs all have synergistic effect to lepidopterous insects such as diamond-back moth, cabbage caterpillar.The present invention is particularly suitable on the crops such as wild cabbage, cauliflower, Broccoliflorets, Chinese cabbage, little green vegetables control diamond-back moth in each, cabbage caterpillar larva in age, and the noctuid pieces of an egg of water spinach, tealeaves crop are had killing action.The present invention also provides Osthole and the composite application of biopesticide on agricultural of bacillus thuringiensis,Bt, particularly prevent and treat the application study of lepidoptera pest, as be used to prevent and treat diamond-back moth, cabbage caterpillar larva and the water spinach of wild cabbage, cauliflower, Broccoliflorets, Chinese cabbage, little green vegetables crop, the noctuid of tealeaves crop.Described Osthole is from the preparation of various active constituent contents, and bacillus thuringiensis,Bt is from the former powder, pulvis or the aqua that contain insecticidal activity albumen bacillus thuringiensis,Bt.
Detailed description of the Invention
The agent of Osthole list has the better prevention effect to diamond-back moth, cabbage caterpillar, and Osthole and B.t carry out composite, has following advantage:
1, show as synergy, owing to B.t has longevity of residure weak point, acts on shortcomings such as slow, and Osthole comparatively speaking, and it is better that speed is killed property, and both are composite, can have complementary advantages, and show as synergy;
2, the consumption that has reduced Osthole is compared with single agent in composite back, reduces the dependence to plant resources;
3, can administer the pesticide resistance of chrysanthemum ester class medicament such as cypermethrin lepidoptera pest diamond-back moth, cabbage caterpillar etc. after composite, can reduce the field using dosage simultaneously, thereby reduce the selection pressure of insect this type of medicament.
Osthole used in the present invention can extract acquisition with existing extraction process, can directly buy from the market to obtain.The microorganism insecticide bacillus thuringiensis,Bt composite with it is to obtain by fermentation, at present, has many companies to produce this medicine on the market, and wide material sources are as long as meet proportion of the present invention and application conditions.
Be elaborated to Osthole and with composite proportion and the controlling object of bacillus thuringiensis,Bt below with synergistic effect; these contents just are used for explanation; and do not mean that protection scope of the present invention limited that wherein said active component is all in active ingredient.
1, Toxicity Determination and compound proportion screening:
1.1 for trying insect:
Diamond-back moth (Plutella xylostella L), cabbage caterpillar (Pieris rapae).
1.2 reagent agent:
95% Osthole (its English name of following usefulness Osthol), biological products company of the high section of Chengdu, Sichuan dragon's fountain provides, and is dissolved in the methyl-sulfoxide, makes 1000 μ g/mL mother liquors;
(32000IU/mgB.t IU is the virulence international unit of B.t), the green peaceful thing agricultural chemicals in Pucheng, Fujian Co., Ltd is dissolved in sterile water, makes 1000 μ g/mL mother liquors.
1.3 assay method and composite proportion design:
1.3.1 for trying material:
2 instar larvaes that the indoors artificial that diamond-back moth is provided by Yangzhou Lv Yuan experiment insecticide factory is cultivated, artificial propagation 29 generations.2～3 age cabbage caterpillar raise ground for picking up from academy of agricultural sciences, Jiangsu Province experiment with worm.
1.3.2 composite proportion design and the preparation of pastille dish leaf:
Virulence curve (it is as described below to give birth to the survey method) by preliminary experiment and single agent of being measured carries out proportion design, carries out composite according to the compound proportion of table 1.It is standby that former medicine and composite dose are diluted to the series concentration soup according to table 2.If being blank, clear water measures susceptibility to diamond-back moth and cabbage caterpillar.Fresh cabbage leaves is soaked into 2s in different soups, put into culture dish after drying naturally.
Table 1 Osthole (A) and B.t (B) compound proportion scope and composite illustration (mass ratio)
| Former medicine | Compound proportion scope A: B | Composite illustration | ||||
| Ratio 1 | Ratio 2 | Ratio 3 | Ratio 4 | Ratio 5 | ||
| Osthol/ B.t | 1∶1～60 | 1∶60 | 1∶50 | 1∶25 | 1∶10 | 1∶5 |
Each medicine series diluted concentration gradient of table 2
| Medicament | Diluted concentration gradient (μ g/mL) | ||||
| 95％Osthol | 200 | 100 | 50 | 25 | 12.5 |
| 32000IU/mg B.t | 150 | 125 | 100 | 75 | 50 |
| 95％Osthol/ 32000IU/mg B.t | 200 | 100 | 50 | 25 | 12.5 |
1.3.3 test is handled:
Put into each 30 of 3.1 gained diamond-back moths, cabbage caterpillars in each culture dish that 1.3.2 prepares respectively, each drug concentration is handled 4 times and is repeated, and places 25 ℃ of constant incubators (under the dark condition), checks borer population alive, dead after 48 hours, calculates LC
50, virulence regression equation and co-toxicity coefficient (CTC).
1.4 computational methods
Convert corrected mortality to probit value.Simultaneously concentration conversion is become logarithm, Y=a+bx calculates the lethal concentration of 50 (LC with the virulence formula
50), analyze the effect be mixed with co-toxicity coefficient (CTC).
Lethality (%)=(dead individual number ÷ is for the total borer population of examination) * 100%
Corrected mortality (%)=[(X-Y) ÷ X] * 100%
(X, Y represent survival rate and the interior survival rate of processed group in the control group respectively)
The actual measurement toxicity index ATI of mixture
The theoretical toxicity index TTI of mixture
TTI=(toxicity index of the medicament A * percentage composition of medicament A in mixture)+
(toxicity index of the medicament B * percentage composition of medicament B in mixture)
Has antagonism after CTC≤two kinds of single agent of 80 expressions are mixed;
Has summation action after 80＜CTC＜two kinds of single agent of 120 expressions are mixed;
Has synergistic effect after CTT 〉=two kinds of single agent of 120 expressions are mixed.
1.5 result and analysis
The single agent of Osthol, B.t sees Table 3,4 to the measurement result of diamond-back moth, cabbage caterpillar.Osthol, B.t and five compound proportions thereof the results are shown in Table 5,6,7,8 to the toxicity test of diamond-back moth, cabbage caterpillar.The result shows: B.t: Osthol=25: 1 proportioning is 311 to the co-toxicity coefficient CTC of diamond-back moth, is 392 to the co-toxicity coefficient CTC of cabbage caterpillar, is higher than other four proportionings, has best synergistic effect in 5 proportionings setting.
Two kinds of single agent of table 3 are to the measurement result of diamond-back moth
| 95％Osthol | Concentration of treatment (μ g/mL) | 200 | 100 | 50 | 25 | 12.5 | Contrast |
| Lethality (%) * | 86.67 | 73.33 | 60.00 | 40.00 | 20.00 | 6.67 | |
| Corrected mortality (%) | 85.71 | 71.43 | 57.14 | 35.71 | 14.29 | / | |
| 32000IU/mg B.t | Concentration of treatment (μ g/mL) | 150 | 125 | 100 | 75 | 50 | Contrast |
| Lethality (%) * | 88.33 | 82.50 | 69.17 | 63.33 | 45.83 | 5.00 | |
| Corrected mortality (%) | 87.72 | 81.58 | 67.54 | 61.40 | 42.98 | / |
*Be the mean value of 4 repetitions
Two kinds of single agent of table 4 are to the measurement result of cabbage caterpillar
| 95％Osthol | Concentration of treatment (μ g/mL) | 200 | 100 | 50 | 25 | 12.5 | Contrast |
| Lethality (%) * | 90.00 | 78.33 | 65.83 | 43.33 | 27.50 | 6.67 | |
| Corrected mortality (%) | 89.29 | 76.79 | 63.39 | 39.29 | 22.32 | / | |
| 32000IU/mg B.t | Concentration of treatment (μ g/mL) | 150 | 125 | 100 | 75 | 50 | Contrast |
| Lethality (%) * | 91.67 | 85.00 | 77.50 | 65.83 | 52.50 | 3.33 | |
| Corrected mortality (%) | 91.38 | 84.48 | 76.72 | 64.66 | 50.86 | / |
*Be the mean value of 4 repetitions
The different compound proportions with Osthol of table 5B.t are to the measurement result of diamond-back moth
| Different compound proportions | Concentration of treatment (μ g/mL) | Contrast | 200 | 100 | 50 | 25 | 12.5 |
| Proportioning X 1 60∶1 | Lethality (%) * | 1.67 | 95.00 | 88.33 | 57.50 | 49.17 | 40.00 |
| Corrected mortality (%) | / | 94.92 | 88.14 | 56.78 | 48.31 | 38.98 | |
| Proportioning X 2 50∶1 | Lethality (%) * | 1.67 | 87.50 | 73.33 | 59.17 | 54.17 | 45.00 |
| Corrected mortality (%) | / | 87.29 | 72.88 | 58.47 | 53.39 | 44.07 | |
| Proportioning X 3 25∶1 | Lethality (%) * | 1.67 | 94.17 | 82.50 | 64.17 | 56.67 | 46.67 |
| Corrected mortality (%) | / | 94.07 | 82.20 | 63.56 | 55.93 | 45.76 | |
| Proportioning X 4 10∶1 | Lethality (%) * | 1.67 | 93.33 | 81.67 | 65.00 | 51.67 | 40.83 |
| Corrected mortality (%) | / | 93.22 | 81.36 | 64.41 | 50.85 | 39.83 | |
| Proportioning X 5 5∶1 | Lethality (%) * | 1.67 | 91.67 | 72.50 | 65.83 | 48.33 | 41.67 |
| Corrected mortality (%) | / | 91.53 | 72.03 | 65.25 | 47.46 | 40.68 |
*Be the mean value of 4 repetitions
Table 6 B.t, Osthol and different proportioning thereof are composite to the interpretation of result of diamond-back moth Toxicity Determination
| The medicament title | Virulence regression equation | Coefficient R | Lethal concentration of 50 LC 50 (μg/mL) | Theoretical lethal concentration of 50 LC 50 (μg/mL) | Co-toxicity coefficient CTC |
| B.t | Y＝1.130+2.192x | 0.967 | 58.25 | / | / |
| Osthol | Y＝2.176+1.714x | 0.994 | 44.41 | / | / |
| Proportioning X 1 60∶1 | Y＝2.830+1.565x | 0.962 | 24.37 | 10.24 | 238 |
| Proportioning X 2 50∶1 | Y＝3.689+0.988x | 0.969 | 21.21 | 7.77 | 273 |
| Proportioning X 3 25∶1 | Y＝3.401+1.262x | 0.971 | 18.48 | 5.94 | 311 |
| Proportioning X 4 10∶1 | Y＝3.125+1.389x | 0.987 | 22.38 | 8.85 | 253 |
| Proportioning X 5 5∶1 | Y＝3.343+1.203x | 0.966 | 23.86 | 10.28 | 232 |
The different compound proportions with Osthol of table 7B.t are to the cabbage caterpillar measurement result
| Different compound proportions | Concentration of treatment (μ g/mL) | Contrast (0) | 200 | 100 | 50 | 25 | 12.5 |
| Proportioning X 1 60∶1 | Lethality (%) * | 1.67 | 95.00 | 80.00 | 67.50 | 53.33 | 47.50 |
| Corrected mortality (%) | / | 94.92 | 79.66 | 66.95 | 52.54 | 46.61 | |
| Proportioning X 2 50∶1 | Lethality (%) * | 1.67 | 95.83 | 83.33 | 69.17 | 59.17 | 46.67 |
| Corrected mortality (%) | / | 95.76 | 83.05 | 68.64 | 58.47 | 45.76 | |
| Proportioning X 3 25∶1 | Lethality (%) * | 1.67 | 96.67 | 90.00 | 81.67 | 64.17 | 54.17 |
| Corrected mortality (%) | / | 96.61 | 89.83 | 81.36 | 63.56 | 53.39 | |
| Proportioning X 4 10∶1 | Lethality (%) * | 1.67 | 94.17 | 88.33 | 69.17 | 57.50 | 49.17 |
| Corrected mortality (%) | / | 94.07 | 88.14 | 68.64 | 56.78 | 48.31 | |
| Proportioning X 5 5∶1 | Lethality (%) * | 1.67 | 93.33 | 77.50 | 70.00 | 52.50 | 45.83 |
| Corrected mortality (%) | / | 93.22 | 77.12 | 69.49 | 51.69 | 44.92 |
*Be the mean value of 4 repetitions
Table 8B.t, Osthol and different proportioning thereof are composite to the interpretation of result of cabbage caterpillar Toxicity Determination
| The medicament title | Virulence regression equation | Coefficient R | Lethal concentration of 50 LC 50 (μg/mL) | Theoretical lethal concentration of 50 LC 50 (μg/mL) | Co-toxicity coefficient CTC |
| B.t | Y＝1.388+2.145x | 0.974 | 48.32 | / | / |
| Osthol | Y＝2.420+1.671x | 0.998 | 34.95 | / | / |
| Proportioning X 1：60∶1 | Y＝3.397+1.260x | 0.964 | 18.69 | 7.27 | 257 |
| Proportioning X 2：50∶1 | Y＝3.358+1.331x | 0.976 | 17.13 | 6.12 | 280 |
| Proportioning X 3：25∶1 | Y＝3.462+1.418x | 0.994 | 12.14 | 3.10 | 392 |
| Proportioning X 4：10∶1 | Y＝3.370+1.343x | 0.982 | 16.40 | 5.75 | 285 |
| Proportioning X 5：5∶1 | Y＝3.430+1.223x | 0.972 | 19.21 | 8.14 | 236 |
2, field plot trial:
Filter out B.t according to indoor prescription screening experiment: the proportioning of Osthol=25mg: 1mg, when Osthol was 1mg, then B.t was 8 * 10
5IU, i.e. 25mg * 32000 IU/mg.We have researched and developed 1% Osthole 8000IU/ μ LB.t water suspension emulsion (hereinafter to be referred as No. 1, Osthole), and wherein 70% solvent is a pure water.Wherein in No. 1 preparation of 1mL Osthole, contain Osthole 10mg approximately, B.t 8 * 10
6IU (virulence international unit).According to the proportioning of B.t: Osthol=10mg: 1mg, make 1% Osthole 3200IU/ μ L B.t water suspension emulsion and make the field comparative trial in addition.
The complex preparation field control effectiveness test of Osthole and Bt
2.1 small plot experiment design:
" pesticide field efficacy medicine test criterion " according to the chief editor of The Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC designs the field control effectiveness test of complex preparation to diamond-back moth and cabbage caterpillar.
Concrete experimental scheme sees Table 9.
The design of table 9 field control effectiveness test medicament
| For studying thing | Controlling object | Test medicine | The contrast medicament |
| Head cabbage varieties: H-11 | Diamond-back moth | 1% osthole water-emulsion agent | 10% r |
| 16000IU/mg B.t water dispersion granule | |||
| Composite dose 300 of Osthole and B.t * | |||
| Composite dose 400 of Osthole and B.t * | |||
| Composite dose 500 of 1 Osthole and B.t * | |||
| Head cabbage varieties: H-11 | Cabbage caterpillar | 1% osthole water-emulsion agent | 10% r |
| 16000IU/mg B.t water dispersion granule | |||
| Composite dose 300 of Osthole and B.t * | |||
| Composite dose 400 of Osthole and B.t * | |||
| Composite dose 500 of Osthole and B.t * | |||
| The clear water contrast | |||
The sub-district design:
7 processing are established in test altogether, and every processing repeats for four times, amounts to 28 sub-districts, every sub-district area 30m
2, each sub-district is by district's group arrangement at random.With workers and peasants' 16 type sprayer dispensers.
2.2 test medicine:
1% osthole water-emulsion agent 200 *, the Jiangsu Province Agriculture Science Institute development, valid density is 50 μ g/mL.
16000IU/mg B.t water dispersion granule, the green peaceful thing agricultural chemicals in Pucheng, Fujian Co., Ltd produces, and effective content is 60g/667m
2
Composite dose of Osthole and B.t, the Jiangsu Province Agriculture Science Institute development, wherein Osthole valid density is respectively 33.3 μ g/mL, 25 μ g/mL, 20 μ g/mL.
The contrast medicament:
10% r, Jiangsu Chemical Pesticide Group Co. Ltd produces, and effective content is 45g.ai/hm
2
Blank: clear water.
2.3 investigation method:
Every sub-district fixed point investigation 12 strain wild cabbages write down the larva number alive on whole the wild cabbage.Investigation insect population radix before the medicine was investigated the residual worm amount in every sub-district, calculation correction preventive effect in 3 days, 7 days behind the medicine.Growth has no adverse effects to wild cabbage to follow the tracks of the viewing test medicament behind the medicine, to determine the safety of medicament.Adopt the DMRT statistical method that data are analyzed, with lowercase a, b, c are illustrated in the significance of difference on 0.05 level in the result.
The drug effect computational methods:
Influence to crop:
Check whether medicament has poisoning to crop, will write down the type and the extent of injury of poisoning when poisoning is arranged.In addition, any wholesome effect also should write down (increasing growing way etc.)
Write down poisoning in the following manner:
If a poisoning can be counted or measure, available absolute value representation is as plant height.
Under b, other situations, the extent of damage, available following two kinds of method records:
1. with reference to the poisoning grade scale, write down every sub-district poisoning situation, with-,+, ++, +++, ++ ++ expression.
-: no poisoning;
+: slight poisoning does not influence the crop normal growth;
++: slight poisoning, can restore, can not cause crop failure;
Obviously poisoning of +++: influences the crop normal growth, and crop yield and quality are caused to a certain degree loss;
++ ++: serious poisoning, plant growth is obstructed, and output and mass loss are serious.
2. each handles sub-district with blank relatively and estimate the poisoning percentage.
Simultaneously, the symptom (dwarfing, chlorosis, deformity etc.) of accurate description crop damage.
2.4 result of the test
1% Osthole 8000IU/ μ L B.t water suspension emulsion control brassicaceous vegetable diamond-back moth field control effectiveness test the results are shown in Table 10.Can find out that from table 10 3,7 days preventive effect is respectively 81.63%, 83.39% behind 300 times, 400 times and 500 times three processing medicines of this medicine; 78.62%, 81.77%; 72.34% and 75.74%; 1% Osthole 3200IU/ μ L B.t water suspension emulsion control brassicaceous vegetable diamond-back moth field control effectiveness test the results are shown in Table 12.Can find out that from table 12 3,7 days preventive effect is respectively 79.71%, 82.78% behind 300 times, 400 times and 500 times three processing medicines of this medicine; 74.28%, 77.46%; 66.08% and 69.45%; Medicament 16000IU/mg B.t 60 gram/667m
2, 200 times of 1% osthole water-emulsion agent and 10% r 45g.ai/hm
2, 3,7 days preventive effect is respectively 65.80%, 68.72% behind the medicine; 73.34%, 77.85%; 55.07%, 62.85%.Significance test shows, behind the medicine 3 days, 300 times, 400 times preventive effects of 1% Osthole 8000IU/ μ L B.t water suspension emulsion were significantly higher than other medicaments.Behind the medicine 7 days, 300 times, 400 times preventive effects of this medicine also were significantly higher than other medicaments.Behind the medicine 3,7 days, 300 times, 400 times preventive effects of 1% Osthole 3200IU/ μ L B.t water suspension emulsion were higher than contrast medicament 16000IU/mg B.t and 10% r significantly.
1% Osthole 8000IU/ μ L B.t water suspension emulsion control brassicaceous vegetable cabbage caterpillar field control effectiveness test the results are shown in Table 11.Can find out that from table 11 3,7 days preventive effect is respectively 84.98%, 94.25% behind 300 times, 400 times and 500 times three processing medicines of this medicine; 82.86%, 90.64%; 80.25% and 85.82%.1% Osthole 8000IU/ μ L B.t water suspension emulsion control brassicaceous vegetable cabbage caterpillar field control effectiveness test the results are shown in Table 13.Can find out that from table 13 3,7 days preventive effect is respectively 85.13%, 90.15% behind 300 times, 400 times and 500 times three processing medicines of this medicine; 83.10%, 87.99%; 75.22% and 78.09%; Medicament 16000IU/mg B.t60g/667m
2, 200 times of 1% osthole water-emulsion agent and 10% r 45g.ai/hm
2, 3,7 days preventive effect is respectively 80.78%, 87.46% behind the medicine; 78.25%, 82.32%; 88.41%, 86.38%.Significance test shows, behind the medicine 3 days, 300 times of preventive effects of 1% Osthole 8000IU/ μ L B.t water suspension emulsion were significantly higher than other medicaments, and behind the medicine 7 days, 300 times of this medicines, 400 times of preventive effects are significantly higher than 10% r 45g.ai/hm
2Preventive effect.Behind the medicine 3,7 days, 300 times of preventive effects of 1% Osthole 3200IU/ μ L B.t water suspension emulsion were suitable with the contrast medicament.
2.5 safety evaluatio to crop:
According to observing after the dispenser, the wild cabbage growth of each treatment region is normal, does not find poisoning, and growth has no adverse effects each test medicine to wild cabbage.Conclusion:
1. can find out by field trial, 1% Osthole 8000IU/ μ L B.t water suspension emulsion all is better than 1% Osthole 3200IU/ μ L B.t water suspension emulsion to the preventive effect of diamond-back moth and cabbage caterpillar, so select 1% Osthole 8000IU/ μ L B.t water suspension emulsion as the exploitation formulation.
The lasting period of 2.1% Osthole 8000IU/ μ L B.t water suspension emulsion control diamond-back moth, cabbage caterpillar reached more than 7 days.Behind the medicine 7 days, the preventive effect that 300～400 times of soups are handled diamond-back moth, cabbage caterpillar was 81.77%～83.39%; 90.64%～94.25%.
It is 300～400 times that 3.1% Osthole 8000IU/ μ L B.t water suspension emulsion control diamond-back moth, cabbage caterpillar are recommended working concentration, conventional spraying.
4.1% Osthole 8000IU/ μ L B.t water suspension emulsion control diamond-back moth medication optimum period, should be grasped to contain at the diamond-back moth low instar larvae and send out a phase dispenser; Cabbage caterpillar medication optimum period, should be grasped to contain at cabbage caterpillar 1,2 instar larvaes and send out a phase dispenser.
5.1% Osthole 8000IU/ μ L B.t water suspension emulsion to not producing poisoning for the examination wild cabbage, can be used safely in the test dose scope.
The interpretation of result of the table 101% Osthole 8000IU/ μ L B.t aqueous suspension agent control diamond-back moth test of pesticide effectiveness
| Handle | Behind the medicine 3 days | Behind the medicine 7 days | Poisoning | ||
| Average preventive effect *(％) | Significance 0.05 | Average preventive effect * (％) | Significance 0.05 | ||
| 300 times of 1% Osthole 8000IU/ μ L B.t | 81.63 | a | 83.39 | a | - |
| 400 times of 1% Osthole 8000IU/ μ L B.t | 78.62 | a | 81.77 | a | - |
| 500 times of 1% Osthole 8000IU/ μ L B.t | 72.34 | ab | 75.74 | b | - |
| 16000IU/mg B.t60 gram/667m 2 | 65.80 | bc | 68.72 | c | - |
| 200 times of 1% osthole water-emulsion agent | 73.34 | ab | 77.85 | b | - |
| 10% r, 45 g.ai/hm 2 | 55.07 | c | 62.85 | c | - |
*Be the mean value of four repetitions, a, b, c are illustrated in the significance of difference on 0.05 level.
The interpretation of result of the table 111% Osthole 8000IU/ μ L B.t aqueous suspension agent control cabbage caterpillar test of pesticide effectiveness
| Handle | Behind the medicine 3 days | Behind the medicine 7 days | Poisoning | ||
| Average preventive effect * (％) | Significance 0.05 | Average preventive effect * (％) | Significance 0.05 | ||
| 300 times of 1% Osthole 8000IU/ μ L B.t | 84.98 | a | 94.25 | a | - |
| 400 times of 1% Osthole 8000IU/ μ L B.t | 82.86 | ab | 90.64 | a | - |
| 500 times of 1% Osthole 8000IU/ μ L B.t | 80.25 | ab | 85.82 | ab | - |
| 16000IU/mg B.t60 gram/667m 2 | 80.78 | ab | 87.46 | ab | - |
| 200 times of 1% osthole water-emulsion agent | 78.25 | b | 82.32 | b | - |
| 10% r 45g.ai/hm 2 | 88.41 | a | 86.38 | ab | - |
*Be the mean value of four repetitions, a, b, c are illustrated in the significance of difference on 0.05 level.
The interpretation of result of the table 121% Osthole 3200IU/ μ L B.t aqueous suspension agent control diamond-back moth test of pesticide effectiveness
| Handle | Behind the medicine 3 days | Behind the medicine 7 days | Poisoning | ||
| Average preventive effect *(％) | Significance 0.05 | Average preventive effect * (％) | Significance 0.05 | ||
| 300 times of 1% Osthole 3200IU/ μ L B.t | 79.71 | a | 82.78 | a | - |
| 400 times of 1% Osthole 3200IU/ μ L B.t | 74.28 | a | 77.46 | ab | - |
| 500 times of 1% Osthole 3200IU/ μ L B.t | 66.08 | b | 69.45 | b | - |
| 16000IU/mg B.t60 gram/667m 2 | 62.82 | bc | 67.52 | bc | - |
| 200 times of 1% osthole water-emulsion agent | 73.34 | a | 77.85 | ab | - |
| 10% r, 45 g.ai/hm 2 | 55.07 | c | 62.85 | c | - |
*Be the mean value of four repetitions, a, b, c are illustrated in the significance of difference on 0.05 level.
The interpretation of result of the table 131% Osthole 3200IU/ μ L B.t aqueous suspension agent control cabbage caterpillar test of pesticide effectiveness
| Handle | Behind the medicine 3 days | Behind the medicine 7 days | Poisoning | ||
| Average preventive effect * (％) | Significance 0.05 | Average preventive effect * (％) | Significance 0.05 | ||
| 300 times of 1% Osthole 3200IU/ μ L B.t | 85.13 | ab | 90.15 | a | - |
| 400 times of 1% Osthole 3200IU/ μ L B.t | 83.10 | ab | 87.99 | ab | - |
| 500 times of 1% Osthole 3200IU/ μ L B.t | 75.22 | b | 78.09 | b | - |
| 16000IU/mg B.t60 gram/667m 2 | 80.78 | ab | 87.46 | ab | - |
| 200 times of 1% osthole water-emulsion agent | 78.25 | b | 82.32 | ab | - |
| 10% r 45g.ai/hm 2 | 88.41 | a | 86.38 | ab | - |
*Be the mean value of four repetitions, a, b, c are illustrated in the significance of difference on 0.05 level.
3, noctuid is killed the pieces of an egg effect:
This formulation is when diamond-back moth and cabbage caterpillar effect are killed in the field use, discovery has ovicidal action to the noctuid pieces of an egg, mainly is to make the pieces of an egg villus shedding, thus the ovum abscission, compare with the contrast pieces of an egg, have only 2.5%～10% ovum can hatch larva harm on the pieces of an egg of medicament as long as touch.Because touching the pieces of an egg of medicament, pesticide application technology then can normally not hatch.Be to hatch the larva number in the pieces of an egg according to spray medicine all investigation after 5 days to calculate the preventive effect of pieces of an egg below.
Investigation method: spray medicine after 5 days, the pieces of an egg newly hatched larvae number in random searching 20 strains of every sub-district.
Computational methods: kill ovum effect=[1-(treatment region newly hatched larvae number/check plot newly hatched larvae number)] * 100
Table 121% Osthole 8000IU/ μ L B.t aqueous suspension agent kills noctuid pieces of an egg effect
| Handle | Behind the medicine 5 days | |
| Average preventive effect * (％) | Significance 0.05 | |
| 300 times of 1% Osthole 8000IU/ μ L B.t | 81.23 | a |
| 400 times of 1% Osthole 8000IU/ μ L B.t | 70.13 | ab |
| 500 times of 1% Osthole 8000IU/ μ L B.t | 64.21 | ab |
| 16000IU/mg B.t60 gram/667m 2 | 0.0 | |
| 200 times of 1% osthole water-emulsion agent | 85.15 | a |
| 10% r 45g.ai/hm 2 | 0.0 | |
Because little report that kills the effect of noctuid pieces of an egg therefore in this manual, finally plays ovicidal action to show it by acting on villus shedding on the pieces of an egg on producing.From Osthole the antibacterial mechanism of plant pathogenic fungi is analyzed, it may be the same with inhibition disease fungus chitin deposition, the chitin deposition that also suppresses pieces of an egg fine hair, thereby make the villus shedding that covers pieces of an egg, the ovum grain comes off thereupon, cause the larva number that pieces of an egg can be hatched on the blade to have only 5～20 at last, and the pieces of an egg of contrast normally hatch 80～200 larvas.4, pharmacy security test
Entrust and specify the Nanjing Medical University of unit that carries out the toxicity of pesticide test to measure.Toxicity test result shows that 1% Osthole 8000IU/ μ LB.t water suspension emulsion belongs to the micro-virus kind agricultural chemicals:
The rat acute per os, through skin LD
50: ♂＞5000mg/kg, ♀＞5000mg/kg;
Lagophthalmos stimulates integration: 0 (1h), is 0 behind 48 h, to the eye nonirritant;
The rabbit skin stimulates integration: 0 (4h), to the skin nonirritant.
Claims (4)
1, fructus cnidii thuricin complex insecticide, wherein the mass ratio of Osthole and bacillus thuringiensis,Bt is 1: 1-60.
2, fructus cnidii thuricin complex insecticide as claimed in claim 1, wherein the mass ratio of Osthole and bacillus thuringiensis,Bt is 1: 1-25.
3, fructus cnidii thuricin complex insecticide as claimed in claim 1 or 2, wherein said bacillus thuringiensis,Bt is from the former powder, pulvis or the aqua that contain insecticidal activity albumen bacillus thuringiensis,Bt.
4, the application of any one described fructus cnidii thuricin complex insecticide among the claim 1-3 is used to prevent and treat diamond-back moth, cabbage caterpillar larva of wild cabbage, Brassica oleracea var. botrytis L., Broccoliflorets, Chinese cabbage, little green vegetables crop and the noctuid that is used to prevent and treat water spinach, tealeaves crop.
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| CN101331876B (en) * | 2006-12-11 | 2010-12-29 | 江苏省农业科学院 | Use of osthole compound thing in preparing pesticide for preventing and curing rice green smut |
| CN100409747C (en) * | 2006-12-11 | 2008-08-13 | 江苏省农业科学院 | Application of osthole and its re-compound in preparing pesticide for preventing and controlling rice green smut |
| CN104186495B (en) * | 2014-08-20 | 2016-05-04 | 青岛农业大学 | A kind of SYP-Zo48-Osthole suspended emulsion and preparation method thereof |
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| CN1329825A (en) * | 2001-08-08 | 2002-01-09 | 江苏省苏科植物保护技术开发中心 | Application of cnidiadin in agriculture |
| CN1482123A (en) * | 2003-07-16 | 2004-03-17 | 西安绿泉生物技术有限公司 | Preparation method for cnidium fruit |
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| CN1482123A (en) * | 2003-07-16 | 2004-03-17 | 西安绿泉生物技术有限公司 | Preparation method for cnidium fruit |
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