CN100345961C - Photosynthetic bacterium purification and stabilization process - Google Patents
Photosynthetic bacterium purification and stabilization process Download PDFInfo
- Publication number
- CN100345961C CN100345961C CNB2003101124840A CN200310112484A CN100345961C CN 100345961 C CN100345961 C CN 100345961C CN B2003101124840 A CNB2003101124840 A CN B2003101124840A CN 200310112484 A CN200310112484 A CN 200310112484A CN 100345961 C CN100345961 C CN 100345961C
- Authority
- CN
- China
- Prior art keywords
- purifying
- photosynthetic bacterium
- rhodospirillum
- purity
- pvc plastic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention relates to technology for purifying and stabilizing photosynthetic bacteria. The present invention is characterized in that the technology comprises the following steps: a photosynthetic bacteria purifying and stabilizing workshop of which the illumination intensity and the temperature can be controlled is established; a container for purifying and stabilizing the photosynthetic bacteria is installed, and a certain number of PVC plastic plates are arranged in the container; the photosynthetic bacteria are cultured by adopting the existing method and technology; when the optical density OD660 of culture solution reaches 1.0 to 1.2, the culture solution is pumped into a purifying and stabilizing pool; the temperature of the purifying and stabilizing workshop is maintained in a range of 25 to 30 DEG C, and the illumination intensity is from 2000 to 3000 lux. An index is detected after 2 to 3 days; when the optical density OD660 is larger than 2.0, and the pH value is from 7.0 to 7.5, the content of viable bacteria is detected, and the purity of rhodospirillum is calculated; when the quantity of the rhodospirillum reaches 10<9>CFU/ml, and the purity reaches 95%, the culture solution can serve as finished products for package and preservation. The present invention greatly reduces chloronema giganteum in the culture solution, and enhances the purity and the concentration of the rhodospirillum; accordingly, the quality level of the photosynthetic bacteria is improved.
Description
Affiliated technical field
The present invention relates to the purifying and the stabilization process of a kind of technology, particularly a kind of photosynthetic bacterium.Product is the microbial preparation (liquid) that is mainly used in improvement cultivation water environment and landscape water environment.
Background technology
Photosynthetic bacterium (PSB, Photosynthetic Bacteria) is the general name of a bacterioid, have 23 belong to, kind surplus 80, belong to 4 sections: Rhodospirillaceae (Rhodospirillaceae), Chromatiaceae (Chromatiaceae), Chlorobacteriaceae (Chlorobiaceae) and Chloronema Dubinina and Gorlenko section (Chloroflexaceae).Wherein, the bacterium of Chromatiaceae and Chlorobacteriaceae is the photoautotrophy nutritional type, needs carbonic acid gas (as carbon source) and hydrogen sulfide (as photosynthetic hydrogen donor) in the substratum; The bacterium of Rhodospirillaceae is the photoheterotrophy nutritional type, needs low molecule organic matter (as carbon source and hydrogen donor) in the substratum; The bacterium of Chloronema Dubinina and Gorlenko section is the amphitrophy type, and carbonic acid gas and organism can be as its carbon sources.In the water surrounding condition of nature, the material cycle of the photosynthetic bacterium and the ecosystem (carbon, nitrogen, sulphur, phosphorus etc.) has substantial connection, plays an important role in water environmental self-purification, water quality improvement process.In addition, the bait of Chang Zuowei fish, shrimp, shellfish, zooplankton, or fodder additives.Current, what be widely used is some kinds of Rhodospirillaceae, the kind that particularly red false unit cell belongs to.In the factor that influences the photosynthetic bacterium quality product, the purity of rhodospirillum is a critical index; Because it not only is related to the effect of product, also be related to the preservation period length of product.Because the purity of rhodospirillum not enough (purity of currently available products generally is lower than 70%) causes product after the short period of time " blackout, smelly ", so highly purified rhodospirillum is the key that guarantees quality product.A lot of people think that the purity of rhodospirillum just depends on the quantity of green alga, so adopt the optical filtering measure, still are difficult to deal with problems.Our result of study thinks that the purity of rhodospirillum just depends primarily on the quantity of Chloronema Dubinina and Gorlenko in the product, rather than green alga quantity.But existing culture process is difficult to get rid of the syntrophism of rhodospirillum and Chloronema Dubinina and Gorlenko, because this bacterium of two types utilizes same substratum.We further study 2 important discoveries: the first, though rhodospirillum and Chloronema Dubinina and Gorlenko all have motor capacity, rhodospirillum is that the mode that relies on flagellum to move about is moved, and the Chloronema Dubinina and Gorlenko employing is slipped and the spasm mode is moved; The second, Chloronema Dubinina and Gorlenko intense light irradiation (＞6000lux), dominant growth in the environment of high water temperature (＞35 ℃).At above-mentioned different ecological characteristic, we increase a purifying and stabilization process after the photosynthetic bacterium production technique of routine, take appropriate measures the Chloronema Dubinina and Gorlenko in the nutrient solution is enriched on the solid material, well solve the low production difficult problem of purity of rhodospirillum, improved the quality level of photosynthetic bacterium product.
Goal of the invention
The object of the present invention is to provide a kind of Chloronema Dubinina and Gorlenko that significantly reduces in the nutrient solution, improve the purity and the concentration of rhodospirillum, thereby improved purifying and the stabilization process of the photosynthetic bacterium of photosynthetic bacterium product quality level.
Summary of the invention
Purpose of the present invention can reach by following measure: purifying of a kind of photosynthetic bacterium and stabilization process is characterized in that may further comprise the steps:
(a) set up a photosynthetic bacterium purifying and the stable workshop that to control intensity of illumination and temperature;
(b) photosynthetic bacterium purifying and stable container are installed, are furnished with the PVC plastic sheet of some amount in the container;
(c) adopt existent method and technology to cultivate photosynthetic bacterium, as the optical density(OD) OD of nutrient solution
660Reach at 1.0～1.2 o'clock, cultivating liquid pump to purifying and stabilization pond;
(d) temperature in purifying and stable workshop remains on 25～30 ℃, intensity of illumination 2000～3000lux;
(e) after 2～3 days, detect index, as optical density(OD) OD
660Greater than 2.0, the pH value detects viable bacteria content in the time of 7.0～7.5, calculate rhodospirillum purity;
(f) reach 10 when rhodospirillum quantity
9CFU/ml, purity reach 95%, and nutrient solution can be used as finished product packing and preserves.
Purpose of the present invention can also reach by following measure: photosynthetic bacterium purifying and stable workshop area are not less than 50m
2, highly be 2.5～3m.Photosynthetic bacterium purifying and stable container are the cement pit of square shape, and specification is long 2m * wide 2m * high 0.6m, and the spacing of the PVC plastic sheet of arranging in the cement pit is 15～20cm, at the bottom of the PVC plastic sheet contact tank, and perpendicular at the bottom of the pond.The PVC plastic sheet is a rectangle, and long 2m, wide 0.4m, thickness are 2～5mm.
The present invention has following advantage compared to existing technology: increased a purifying and stabilization process, and in purifying and stable container, be furnished with the PVC plastic sheet, nutrient solution is entered in purifying and the stable container, by control illumination and temperature, make the rhodospirillum continued growth, and Chloronema Dubinina and Gorlenko is enriched on the PVC plastic sheet, significantly reduced the Chloronema Dubinina and Gorlenko in the nutrient solution, improve the purity and the concentration of rhodospirillum, thereby improved the quality level of photosynthetic bacterium, obtained live high purity (＞95%), high density (OD
660＞2.0) photosynthetic bacterium product has prolonged shelf time of product, reaches 120 days.
Embodiment
The present invention will now be further detailed embodiment:
Concrete operations technology of the present invention is as follows:
(1) building photosynthetic bacterium purifying and stable workshop.The workshop area generally is not less than 50m
2, the height at 2.5～3m, can regulate and control indoor air temperature and intensity of illumination.
(2) make up a plurality of photosynthetic bacterium purifying and stable container.Container is the cement pit of square shape, and specification is 2 * 2 * 0.6m (length * wide * height).Arrange the PVC plastic sheet in the cement pit, the phase spacing between plastic sheet is 15～20cm, at the bottom of the plastic sheet contact tank, perpendicular at the bottom of the pond.The PVC plastic sheet is the rectangle of 2 * 0.4m (long * wide), and thickness is 2～5mm.
(3) adopt existent method and technology to cultivate photosynthetic bacterium.Optical density(OD) (OD when nutrient solution
660) reach at 1.0～1.2 o'clock, cultivating liquid pump to purifying and stabilization pond.
(4) temperature in purifying and stable workshop remains on about 25～30 ℃, and intensity of illumination is at 2000～3000lux.
(5) about after 2-3 days, detect index.As optical density(OD) (OD
660) greater than 2.0, pH in the time of 7.0～7.5, detect viable bacteria content, calculate rhodospirillum purity.
(6) if rhodospirillum quantity reaches 10
9CFU/ml, purity reach 95%, and nutrient solution can be used as finished product packing and preserves.
Claims (4)
1, the purification process of a kind of photosynthetic bacterium is characterized in that may further comprise the steps:
(a) set up a photosynthetic bacterium purifying and a stable workshop of controlling intensity of illumination and temperature;
(b) photosynthetic bacterium purifying and stable container are installed, are furnished with the PVC plastic sheet in the container;
(c) adopt existent method and technology to cultivate photosynthetic bacterium, when nutrient solution when the light absorption value of wavelength 660nm reaches 1.0～1.2, the cultivation liquid pump to purifying and stabilization pond;
(d) temperature in purifying and stable workshop remains on 25～30 ℃, intensity of illumination 2000～3000lux;
(e) after 2～3 days, greater than 2.0, the pH value detects viable bacteria content to nutrient solution in the time of 7.0～7.5, calculate rhodospirillum purity at the light absorption value of wavelength 660nm;
(f) reach 10 when rhodospirillum quantity
9CFU/ml, purity reach 95%, and nutrient solution is preserved as finished product packing.
2, method according to claim 1 is characterized in that: photosynthetic bacterium purifying and stable workshop area are not less than 50m
2, highly be 2.5～3m.
3, method according to claim 1, it is characterized in that: photosynthetic bacterium purifying and stable container are the cement pit of square shape, and specification is long 2m * wide 2m * high 0.6m, and the spacing of the PVC plastic sheet of arranging in the cement pit is 15～20cm, at the bottom of the PVC plastic sheet contact tank, and perpendicular at the bottom of the pond.
4, method according to claim 1 is characterized in that: the PVC plastic sheet is a rectangle, and long 2m, wide 0.4m, thickness are 2～5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101124840A CN100345961C (en) | 2003-12-08 | 2003-12-08 | Photosynthetic bacterium purification and stabilization process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101124840A CN100345961C (en) | 2003-12-08 | 2003-12-08 | Photosynthetic bacterium purification and stabilization process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1546649A CN1546649A (en) | 2004-11-17 |
| CN100345961C true CN100345961C (en) | 2007-10-31 |
Family
ID=34336545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101124840A Expired - Fee Related CN100345961C (en) | 2003-12-08 | 2003-12-08 | Photosynthetic bacterium purification and stabilization process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100345961C (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2232920Y (en) * | 1995-11-15 | 1996-08-14 | 山西大学 | Device for culture of photosynthesis strain liquid |
| WO2003067213A2 (en) * | 2002-02-04 | 2003-08-14 | The Regents Of The University Of California | Modulation of sulfate permease for photosynthetic hydrogen production |
| CN1441054A (en) * | 2002-02-25 | 2003-09-10 | 赵平初 | Culture pond process of producing photosynthetic bacteria for animal and plant |
-
2003
- 2003-12-08 CN CNB2003101124840A patent/CN100345961C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2232920Y (en) * | 1995-11-15 | 1996-08-14 | 山西大学 | Device for culture of photosynthesis strain liquid |
| WO2003067213A2 (en) * | 2002-02-04 | 2003-08-14 | The Regents Of The University Of California | Modulation of sulfate permease for photosynthetic hydrogen production |
| CN1441054A (en) * | 2002-02-25 | 2003-09-10 | 赵平初 | Culture pond process of producing photosynthetic bacteria for animal and plant |
Non-Patent Citations (2)
| Title |
|---|
| 一株硫氧化细菌的分离与鉴定 刘蔚等，四川大学学报(自然科学版)，第37卷第3期 2000 * |
| 绿色红假单胞菌和绿硫红假单胞菌的分离与鉴定 杨素萍等，微生物学报，第35卷第02期 1995 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1546649A (en) | 2004-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102766578B (en) | Cultivating and producing method for haematococcus pluvialis | |
| Markou et al. | Ammonia inhibition on Arthrospira platensis in relation to the initial biomass density and pH | |
| CN101363005A (en) | Method for coculturing fine algae and photosynthetic bacteria | |
| CN105130012A (en) | Water quality improver | |
| CN102212491A (en) | Simple culture method of photosynthetic bacteria with high cell concentration | |
| Cuello et al. | Comparison of continuous and day time only mixing on Tetraselmis suecica (Chlorophyta) in outdoor raceway ponds | |
| Magdaong et al. | Effect of aeration rate and light cycle on the growth characteristics of Chlorella sorokiniana in a photobioreactor | |
| Tanaka et al. | Low temperatures in dark period affect biomass productivity of a cyanobacterium Arthrospira platensis | |
| Makaroglou et al. | Optimization of biomass production from Stichococcous sp. biofilms coupled to wastewater treatment | |
| Barzee et al. | Pilot microalgae cultivation using food waste digestate with minimal resource inputs | |
| CN105002085A (en) | Culture farm special-purpose microalgae photo-biological culture system and culture method | |
| CN106868085A (en) | A kind of method for promoting haematococcus pluvialis rapid conversion to accumulate astaxanthin | |
| CN106745814A (en) | Compound improver of water quality for aquaculture and preparation method thereof | |
| CN100345961C (en) | Photosynthetic bacterium purification and stabilization process | |
| CN1710060A (en) | Traingular brown algae open culture method and its special culture meidum | |
| CN106480155A (en) | A kind of method being suitable for promoting Haematococcus pluvialis production astaxanthin under the high temperature conditions | |
| CN100564512C (en) | A kind of photosynthetic bacterium enriched substratum | |
| CN1257263C (en) | Process of culturing photosynthetic bacteria with ultrahigh cell density | |
| CN101358178B (en) | Method for culturing photosynthetic bacteria | |
| CN107699493B (en) | Microalgae cultivation method | |
| CA2852815A1 (en) | Method and system for the culture of microalgae | |
| TW201311141A (en) | Microalgae cultivation module | |
| KR100622025B1 (en) | The medium composition for optimum growth and maximum biomass of Spirulina genus | |
| CN1169941C (en) | Method for semiaseptic culturing heterotrophic chlorella | |
| CN101407772B (en) | Method for cultivating photosynthetic bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20071031 Termination date: 20100108 |