CN100336558C - Method for preparing cow foremilk immune globulin microcapsulation - Google Patents
Method for preparing cow foremilk immune globulin microcapsulation Download PDFInfo
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- CN100336558C CN100336558C CNB200510084227XA CN200510084227A CN100336558C CN 100336558 C CN100336558 C CN 100336558C CN B200510084227X A CNB200510084227X A CN B200510084227XA CN 200510084227 A CN200510084227 A CN 200510084227A CN 100336558 C CN100336558 C CN 100336558C
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Abstract
A product of the present invention is used for medical medicines and health foods. The existing problem comprises: the effective bioavailability of immunoglobulin activity is lower because of enzymatic degradation and chemical degradation in the stomach intestine and slow oxidation of common preparation in natural environment. The present invention comprises the steps: bovine colostrum immunoglobulin is pulverized to 40 to 120 meshes, ethyl cellulose is pulverized to 80 to 140 meshes, and the ethyl cellulose is stirred and added in an acetone or acetidin solution to be completely dissolved to obtain a solution a; the pulverized bovine colostrum immunoglobulin is added in the stirred solution a to be dissolved, the mass ratio of the bovine colostrum immunoglobulin to the ethyl cellulose is 0.1:1 to 4:1, and a solution b is obtained and is stirred; liquid paraffin is bathed by water, the water bathing temperature is controlled at 20 to 25 DEG C, an emulsifying agent span-80 is stirred synchronously to be added in, the volume ratio of the emulsifying agent span-80 to the paraffin is 1:800 to 4:800, a solution c is obtained, and the solution b is added in the solution c to be stirred to obtain microcapsules. The present invention obtains the microcapsules which have the advantages of good round and normal degree and adherence degree; furthermore, the grain diameter is from 500 to 900 mu m.
Description
Technical field
The microencapsulation of bovine colostrum immunoglobulin, product are applicable to the composition in Medicines, the health food.
Background technology
The raising of the people's living standard that drives along with the raising of gross national product, people more recognize healthy importance in life, people also more pay close attention to household and crowd's health status at one's side simultaneously.Guarantee healthy optimal path just and improve immunity.Therefore how improving the hot issue that body immunity has become people's special concern after dinner, also is the important topic of actively researching and developing of researchers.Immunoglobulin can be treated multiple disease as the important component part among the human immune system; And contain the functional component that has different physiological actions in a large number in the cattle colostrums.In the functional component of colostrum, it is most important a kind of immune factor that albumen is asked in immunity, and wherein most immunoglobulin is IgG.Clinical research shows that IgG is bringing into play potential, positive immune effect aspect prevention and the treatment in the cattle colostrums.Recently also have research to point out that cattle colostrums IgG has specific antibody activity to the human disease bacterium.
Yet existing bovine colostrum immunoglobulin preparation comprises powder, hard capsule, tablet.These preparations are except characteristics separately, and a total shortcoming is exactly to some extent by acid and enzymatic degradation in gastrointestinal.It is reported, be 2 and 4 o'clock at pH value, and cattle colostrums IgG was through pepsin digestion 2 hours, and its activity preservation rate is respectively 25% and 81.3%; At pH value is 7 o'clock, and cattle colostrums IgG was through trypsinization 2 hours, and its activity preservation rate is 90.6%.Because after human body was taken in cattle colostrums, human body obtained passive immunity, intestinal need not to absorb and just can play a role.Cattle colostrums Ig arrive behind the intestinal if having active just can be specifically in conjunction with the pathogenic bacterium in, the removing intestinal, thereby play the effect of prophylactic treatment disease.Therefore, reducing the preceding degraded of immunoglobulin arrival active site is a significant research work.
At present, the patent of still unmatchful cow foremilk immune globulin microcapsulation aspect research both at home and abroad.Existing subject matter is: immune globulin activity, as the enzymatic degradation in the above-mentioned because gastrointestinal, the eremacausis in natural environment of chemical degradation and general formulation causes effective bioavailability not high, as tablet or hard capsule at disintegrate or softgel shell dissolubility, short time reaches the highest blood drug level value of valid density, makes bioavailability not high.
Summary of the invention
The present invention has realized cow foremilk immune globulin microcapsulation, thereby has improved effective bioavailability.
The preparation method of a kind of cow foremilk immune globulin microcapsulation provided by the invention is characterized in that, may further comprise the steps:
1) bovine colostrum immunoglobulin is crushed to the 40-120 order, ethyl cellulose is crushed to the 80-140 order, at rotating speed is under the 100-500rpm ethyl cellulose to be joined in acetone or the ethyl acetate solution to dissolving fully with the flow velocity of 8-15ml/s, and obtaining solution is a solution;
2) bovine colostrum immunoglobulin after will pulverizing adds in a solution under rotating speed 100-500rpm stirs and dissolves, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 0.1: 1-4: 1, obtaining solution is b solution, and b solution is stirred under rotating speed 100-500rpm;
3) with the liquid paraffin water-bath, the control bath temperature is at 20-50 ℃, add the emulsifying agent span-80 while stirring, emulsifying agent span-80 volume is 1 with the ratio of liquid paraffin volume: 800--4: 800, obtaining solution is c solution, b solution is added in the c solution, stirs with the speed of 100-500rpm and obtain the bovine colostrum immunoglobulin microcapsule.
It is good that the present invention obtains roundness, and degree of adhesion is good, the microcapsule of particle diameter 500-900 μ m.
Description of drawings
The sem photograph of the bovine colostrum immunoglobulin microcapsule of Fig. 1: embodiment 1
The sem photograph of the bovine colostrum immunoglobulin microcapsule of Fig. 2: embodiment 2
The sem photograph of the bovine colostrum immunoglobulin microcapsule of Fig. 3: embodiment 3
The specific embodiment
Embodiment 1: bovine colostrum immunoglobulin is crushed to 40 orders, ethyl cellulose is crushed to 80 orders, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 1: 1, at rotating speed is under the 100rpm ethyl cellulose to be joined in the acetone soln with flow velocity 5-15ml/s to dissolve, and obtaining solution is a solution.The bovine colostrum immunoglobulin of pulverizing added in a solution under rotating speed 100rpm stirs dissolve, obtaining solution is b solution, with b solution under rotating speed 100rpm stirs.As for water-bath in the beaker, the control bath temperature adds the emulsifying agent span-80 while stirring at 20 ℃ with appropriate amount of fluid paraffin, and emulsifying agent span-80 volume is 1: 800 with the ratio of liquid paraffin volume, and b solution is added in the c solution.Speed with 100rpm is stirred to microcapsule formation.Obtain than rounding, relatively the microcapsule of the particle diameter 700-900 μ m of adhesion.
Embodiment 2: bovine colostrum immunoglobulin is crushed to 70 orders, ethyl cellulose is crushed to 110 orders, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 1: 1, at rotating speed is under the 300rpm ethyl cellulose to be joined in the ethyl acetate solution with flow velocity 8-15ml/s to dissolve, and obtaining solution is a solution.The bovine colostrum immunoglobulin of pulverizing added in a solution under rotating speed 300rpm stirs dissolve, obtaining solution is b solution, with b solution under rotating speed 300rpm stirs.As for water-bath in the beaker, the control bath temperature adds the emulsifying agent span-80 while stirring at 20 ℃ with the 800ml liquid paraffin, and emulsifying agent span-80 volume is 3: 800 with the ratio of liquid paraffin volume, and b solution is added in the c solution.Speed with 300rpm is stirred to microcapsule formation.It is good to obtain roundness, and degree of adhesion is good, the microcapsule of particle diameter 600-800 μ m.
Embodiment 3: bovine colostrum immunoglobulin is crushed to 80 orders, ethyl cellulose is crushed to 120 orders, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 1: 1, at rotating speed is under the 400rpm ethyl cellulose to be joined in the acetone soln with flow velocity 16-25ml/s to dissolve, and obtaining solution is a solution.The bovine colostrum immunoglobulin of pulverizing added in a solution under rotating speed 400rpm stirs dissolve, obtaining solution is b solution, with b solution under rotating speed 400rpm stirs.As for water-bath in the beaker, the control bath temperature adds the emulsifying agent span-80 while stirring at 20 ℃ with the 800ml liquid paraffin, and emulsifying agent span-80 volume is 2.5: 1 with the ratio of liquid paraffin volume, and b solution is added in the c solution.Speed with span-80 is stirred to microcapsule formation.It is outstanding to obtain roundness, and degree of adhesion is outstanding, the microcapsule of particle diameter 500-700 μ m.
Embodiment 4: bovine colostrum immunoglobulin is crushed to 70 orders, ethyl cellulose is crushed to 110 orders, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 4: 1, at rotating speed is under the 300rpm ethyl cellulose to be joined in the acetone soln with flow velocity 8-15ml/s to dissolve, and obtaining solution is a solution.The bovine colostrum immunoglobulin of pulverizing added in a solution under rotating speed 300rpm stirs dissolve, obtaining solution is b solution, with b solution under rotating speed 300rpm stirs.As for water-bath in the beaker, the control bath temperature adds the emulsifying agent span-80 while stirring at 20 ℃ with the 800ml liquid paraffin, and emulsifying agent span-80 volume is 3: 800 with the ratio of liquid paraffin volume, and b solution is added in the c solution.Speed with 300rpm is stirred to microcapsule formation.It is good to obtain roundness, and degree of adhesion is good, particle diameter 600-800 μ m microcapsule.
Embodiment 5: bovine colostrum immunoglobulin is crushed to 80 orders, ethyl cellulose is crushed to 120 orders, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 4: 1, at rotating speed is under the 400rpm ethyl cellulose to be joined in the ethyl acetate solution with flow velocity 8-15ml/s to dissolve, and obtaining solution is a solution.The bovine colostrum immunoglobulin of pulverizing added in a solution under rotating speed 400rpm stirs dissolve, obtaining solution is b solution, with b solution under rotating speed 400rpm stirs.As for water-bath in the beaker, the control bath temperature adds the emulsifying agent span-80 while stirring at 20 ℃ with the 800ml liquid paraffin, and emulsifying agent span-80 volume is 3: 800 with the ratio of liquid paraffin volume, and b solution is added in the c solution.Speed with 400rpm is stirred to microcapsule formation.It is good to obtain roundness, and degree of adhesion is good, particle diameter 500-700 μ m microcapsule.
Embodiment 6: bovine colostrum immunoglobulin is crushed to 70 orders, ethyl cellulose is crushed to 110 orders, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 4: 1, at rotating speed is under the 300rpm ethyl cellulose to be joined in the ethyl acetate solution with flow velocity 8-15ml/s to dissolve, and obtaining solution is a solution.The bovine colostrum immunoglobulin of pulverizing added in a solution under rotating speed 300rpm stirs dissolve, obtaining solution is b solution, with b solution under rotating speed 300rpm stirs.As for water-bath in the beaker, the control bath temperature adds the emulsifying agent span-80 while stirring at 20 ℃ with the 800ml liquid paraffin, and emulsifying agent span-80 volume is 3: 800 with the ratio of liquid paraffin volume, and b solution is added in the c solution.Speed with 300rpm is stirred to microcapsule formation.It is better to obtain roundness, and degree of adhesion is good, particle diameter 500-800 μ m microcapsule.
Claims (1)
1, a kind of preparation method of cow foremilk immune globulin microcapsulation is characterized in that, may further comprise the steps:
1) bovine colostrum immunoglobulin is crushed to the 40-80 order, ethyl cellulose is crushed to the 80-140 order, at rotating speed is under the 100-500rpm ethyl cellulose to be joined in acetone or the ethyl acetate solution to dissolving fully with the flow velocity of 8-15ml/s, and obtaining solution is a solution;
2) bovine colostrum immunoglobulin after will pulverizing adds in a solution under rotating speed 100-500rpm stirs and dissolves, bovine colostrum immunoglobulin and ethyl cellulose mass ratio are 0.1: 1-4: 1, obtaining solution is b solution, and b solution is stirred under rotating speed 100-500rpm;
3) with the liquid paraffin water-bath, the control bath temperature is at 20-50 ℃, add the emulsifying agent span-80 while stirring, emulsifying agent span-80 volume is 1 with the ratio of liquid paraffin volume: 800-4: 800, obtaining solution is c solution, b solution is added in the c solution, stirs with the speed of 100-500rpm and obtain the bovine colostrum immunoglobulin microcapsule.
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| CNB200510084227XA CN100336558C (en) | 2005-07-18 | 2005-07-18 | Method for preparing cow foremilk immune globulin microcapsulation |
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| CNB200510084227XA CN100336558C (en) | 2005-07-18 | 2005-07-18 | Method for preparing cow foremilk immune globulin microcapsulation |
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| CN100336558C true CN100336558C (en) | 2007-09-12 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101181632B (en) * | 2007-11-23 | 2010-09-08 | 北京联合大学生物化学工程学院 | Method for preparing superoxide dismutase microcapsule |
| CN107594539B (en) * | 2016-07-11 | 2021-02-02 | 河南工业大学 | Method for preparing microcapsule embedded globulin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4798786A (en) * | 1982-05-06 | 1989-01-17 | Stolle Research And Development Corporation | Living cells encapsulated in crosslinked protein |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4798786A (en) * | 1982-05-06 | 1989-01-17 | Stolle Research And Development Corporation | Living cells encapsulated in crosslinked protein |
Non-Patent Citations (2)
| Title |
|---|
| 微胶囊制备技术及其应用研究 天云等，科学技术与工程，第5卷第1期 2005 * |
| 微胶囊的制备技术 许燕侠等，上海化工，第30卷第3期 2005 * |
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