CL2016003427A1 - Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés. - Google Patents

Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.

Info

Publication number
CL2016003427A1
CL2016003427A1 CL2016003427A CL2016003427A CL2016003427A1 CL 2016003427 A1 CL2016003427 A1 CL 2016003427A1 CL 2016003427 A CL2016003427 A CL 2016003427A CL 2016003427 A CL2016003427 A CL 2016003427A CL 2016003427 A1 CL2016003427 A1 CL 2016003427A1
Authority
CL
Chile
Prior art keywords
peptide
interest
production
host cell
expression vector
Prior art date
Application number
CL2016003427A
Other languages
English (en)
Inventor
Anne Andrews Farrow Bárbara
Alfonso Asenjo De Leuze Juan
Rodríguez Gallardo Vida
Original Assignee
Univ Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Chile filed Critical Univ Chile
Priority to CL2016003427A priority Critical patent/CL2016003427A1/es
Publication of CL2016003427A1 publication Critical patent/CL2016003427A1/es
Priority to US16/474,805 priority patent/US11261472B2/en
Priority to PCT/IB2017/058480 priority patent/WO2018122777A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
CL2016003427A 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés. CL2016003427A1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CL2016003427A CL2016003427A1 (es) 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.
US16/474,805 US11261472B2 (en) 2016-12-30 2017-12-28 Peptide sequence of a guide protein for the production of a peptide of interest, an expression vector, a host cell, and a method for the production of a peptide of interest
PCT/IB2017/058480 WO2018122777A1 (es) 2016-12-30 2017-12-28 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2016003427A CL2016003427A1 (es) 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.

Publications (1)

Publication Number Publication Date
CL2016003427A1 true CL2016003427A1 (es) 2017-06-23

Family

ID=59858446

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2016003427A CL2016003427A1 (es) 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.

Country Status (3)

Country Link
US (1) US11261472B2 (es)
CL (1) CL2016003427A1 (es)
WO (1) WO2018122777A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117164722B (zh) * 2023-08-09 2024-04-09 东北农业大学 一种螺旋束damp4-pr-fo融合蛋白d2l及其制备方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996017942A1 (en) 1994-12-07 1996-06-13 Bionebraska, Inc. Production of peptides using recombinant fusion protein constructs
US7072771B2 (en) * 2001-06-07 2006-07-04 University Of Kentucky Research Foundation Selective PARP-1 targeting for designing chemo/radio sensitizing agents
US20100234568A1 (en) 2006-10-19 2010-09-16 Linda Jane Decarolis Identification of peptide tags for the production of insoluble peptides by sequence scanning
EP2499255A4 (en) 2009-11-09 2013-06-19 Univ Colorado Regents EFFECTIVE PRODUCTION OF PEPTIDES
WO2013142859A2 (en) 2012-03-23 2013-09-26 Wuhan Peptech Pharmaceutical Co., Ltd. Fusion proteins of superfolder green fluorescent protein and use thereof

Also Published As

Publication number Publication date
US11261472B2 (en) 2022-03-01
US20200385774A1 (en) 2020-12-10
WO2018122777A1 (es) 2018-07-05

Similar Documents

Publication Publication Date Title
CO2017005639A2 (es) Anticuerpos anti-miostatina, polipéptidos que contienen regiones fc variantes
DK3292137T3 (da) Proteiner specifikke for cd137
MX2017006127A (es) Metodos y composiciones para produccion de proteina de clara de huevo.
PL3708668T3 (pl) Sztuczne cząsteczki kwasu nukleinowego dla poprawy ekspresji białka
MX2017003847A (es) Proteinas biespecificas activables por proteasas.
DK3402340T3 (da) Fremgangsmåde til fremstilling af højt fordøjeligt hydrolyseret keratinholdigt materiale
IL251571A0 (en) Variable proteins comprising the cry1da1 amino acid sequence are activated by lepidopteran
EP3385305A4 (en) PROCESS FOR PRODUCING A PROTEIN SOLUTION
SG11201701384XA (en) Process for the conjugation of a peptide or protein with a reagent comprising a leaving group including a portion of peg
BR112016024895A2 (pt) fator secretório de proteína com alta eficácia secretória e um vetor de expressão compreendendo o mesmo
EP3312278A4 (en) Protein expression method
EP3360960A4 (en) ANTIBODIES WITH INGREDIENTS OF NON-NATURAL AMINO ACID
DK3262168T3 (da) Værtscelleproteinmodifikation
EP3438262A4 (en) PROCESS FOR PRODUCTION OF PROTEIN
EP3434782A4 (en) PROCESS FOR PRODUCING PROTEINS
EP3564254A4 (en) PROTEIN RECOVERY PROCESS
DK3389701T3 (da) Nucleotidesekvens, der udtrykker et exosomforankrende, protein til anvendelse som vaccine
EP3147369A4 (en) Fibroin-like protein production method
EP3330376A4 (en) ANTI-EVA 1 PROTEIN ANTIBODY
HK1255456A1 (zh) 含fc蛋白的表達
BR112017002139A2 (pt) ?método para preparar um composto?
CL2017001633A1 (es) Método de manufactura de proteína.
CL2016003427A1 (es) Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.
IT201600131935A1 (it) Sequenza segnale di proteina vegetale come coadiuvante in vaccini a DNA.
EP3311152A4 (en) PROBABILITY-BASED SUCCESSORIES FOR LIBRARIES (PROLS)