CL2016001649A1 - Metodo ultrasensible de deteccion de biomarcador de cancer gastrico reprimo - Google Patents
Metodo ultrasensible de deteccion de biomarcador de cancer gastrico reprimoInfo
- Publication number
- CL2016001649A1 CL2016001649A1 CL2016001649A CL2016001649A CL2016001649A1 CL 2016001649 A1 CL2016001649 A1 CL 2016001649A1 CL 2016001649 A CL2016001649 A CL 2016001649A CL 2016001649 A CL2016001649 A CL 2016001649A CL 2016001649 A1 CL2016001649 A1 CL 2016001649A1
- Authority
- CL
- Chile
- Prior art keywords
- repression
- gastric cancer
- biomarker detection
- ultrasensitive method
- oligonucleotide
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Urology & Nephrology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Inorganic Chemistry (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Método que comprende tratar unaa muestra de ADN con bisulfito de sodio; proveer una nanopartícula metálica de entre 40 a 80 nm, que comprende en su superficie un oligonucleótido que reconoce RPRM metilado escogido entre la SEQ ID Nº1 Nº2 y opcionalmente un marcador para fluorescencia, o Raman. Proveer una segunda nanopartícula metálica de entre 8 a 20 nm o un punto cuántico de 2 a 10 nm, que comprende en su superficie el otro oligonucleótido. Hibridar, centrifugar y detectar los híbridos en el sedimento.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562185194P | 2015-06-26 | 2015-06-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2016001649A1 true CL2016001649A1 (es) | 2016-12-30 |
Family
ID=57584397
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2016001649A CL2016001649A1 (es) | 2015-06-26 | 2016-06-24 | Metodo ultrasensible de deteccion de biomarcador de cancer gastrico reprimo |
Country Status (2)
| Country | Link |
|---|---|
| CL (1) | CL2016001649A1 (es) |
| WO (1) | WO2016205971A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108827935B (zh) * | 2018-06-08 | 2021-09-07 | 南京师范大学 | 一种基于金纳米孔阵列的dna甲基化表面增强拉曼散射光谱检测方法及其应用 |
| WO2019241899A1 (es) | 2018-06-20 | 2019-12-26 | Pontificia Universidad Catolica De Chile | Detección no invasiva de cáncer gástrico a través de la detección en sangre de la metilación de reprimo like |
| CN109929919B (zh) * | 2018-09-14 | 2020-02-14 | 深圳市晋百慧生物有限公司 | Dna甲基化检测方法及相关应用 |
| RU2723160C1 (ru) * | 2019-08-15 | 2020-06-09 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Способ обнаружения и определения днк с заданной последовательностью методом спектроскопии гигантского комбинационного рассеяния |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080241828A1 (en) * | 2007-03-30 | 2008-10-02 | Kai Wu | Detection of dna methylation using raman spectroscopy |
| CL2009001907A1 (es) * | 2009-09-28 | 2010-05-28 | Univ Pontificia Catolica Chile | Metodo de deteccion precoz de cancer gastrico que comprende detectar la presencia de marcadores especificos de cancer gastrico en muestras de plasma. |
| WO2015181804A2 (en) * | 2014-05-30 | 2015-12-03 | Pontificia Universidad Católica De Chile | Method and assay for the non-invasive detection of early gastric cancer by the combined use of methylated reprimo cell-free dna and pepsinogen i/ii |
-
2016
- 2016-06-24 WO PCT/CL2016/050035 patent/WO2016205971A1/es active Application Filing
- 2016-06-24 CL CL2016001649A patent/CL2016001649A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016205971A1 (es) | 2016-12-29 |
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