CL2015001674A1 - Regulación negativa de un polinucleótido que codifica una proteina de uso sorbitol sou2 para modificar la produccion de lipidos en células microbianas - Google Patents
Regulación negativa de un polinucleótido que codifica una proteina de uso sorbitol sou2 para modificar la produccion de lipidos en células microbianasInfo
- Publication number
- CL2015001674A1 CL2015001674A1 CL2015001674A CL2015001674A CL2015001674A1 CL 2015001674 A1 CL2015001674 A1 CL 2015001674A1 CL 2015001674 A CL2015001674 A CL 2015001674A CL 2015001674 A CL2015001674 A CL 2015001674A CL 2015001674 A1 CL2015001674 A1 CL 2015001674A1
- Authority
- CL
- Chile
- Prior art keywords
- sou2
- sorbitol
- protein
- polynucleotide encoding
- microbial cells
- Prior art date
Links
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 title 1
- 230000003828 downregulation Effects 0.000 title 1
- 150000002632 lipids Chemical class 0.000 title 1
- 230000000813 microbial effect Effects 0.000 title 1
- 102000040430 polynucleotide Human genes 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 239000000600 sorbitol Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01289—Sorbose reductase (1.1.1.289)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261740506P | 2012-12-21 | 2012-12-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2015001674A1 true CL2015001674A1 (es) | 2015-10-23 |
Family
ID=49885508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2015001674A CL2015001674A1 (es) | 2012-12-21 | 2015-06-16 | Regulación negativa de un polinucleótido que codifica una proteina de uso sorbitol sou2 para modificar la produccion de lipidos en células microbianas |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9005940B2 (es) |
| EP (1) | EP2935591B1 (es) |
| JP (1) | JP2016502851A (es) |
| BR (1) | BR112015014844A2 (es) |
| CA (1) | CA2892516A1 (es) |
| CL (1) | CL2015001674A1 (es) |
| WO (1) | WO2014100061A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2935601T3 (en) * | 2012-12-21 | 2018-06-18 | Du Pont | RECOMBINANT MICROBELL CELLS PRODUCING AT LEAST 28% EICOSAPENTAIC ACID AS DRY WEIGHT |
| JP6898915B2 (ja) | 2015-07-22 | 2021-07-07 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | 生物を用いる長鎖ジカルボン酸の高レベルの生産 |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5071764A (en) | 1983-10-06 | 1991-12-10 | Pfizer Inc. | Process for integrative transformation of yarrowia lipolytica |
| US4880741A (en) | 1983-10-06 | 1989-11-14 | Pfizer Inc. | Process for transformation of Yarrowia lipolytica |
| US6825017B1 (en) | 1998-12-07 | 2004-11-30 | Washington State University Research Foundation | Desaturases and methods of using them for synthesis of polyunsaturated fatty acids |
| AU2004215705B2 (en) | 2003-02-27 | 2009-11-12 | Basf Plant Science Gmbh | Method for the production of polyunsaturated fatty acids |
| US7214491B2 (en) | 2003-05-07 | 2007-05-08 | E. I. Du Pont De Nemours And Company | Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts |
| US7238482B2 (en) | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US8313911B2 (en) | 2003-05-07 | 2012-11-20 | E I Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US7459546B2 (en) | 2003-06-25 | 2008-12-02 | E.I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast |
| US20110059496A1 (en) | 2003-06-25 | 2011-03-10 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
| US7267976B2 (en) | 2003-07-02 | 2007-09-11 | E.I. Du Pont De Nemours And Company | Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts |
| US7504259B2 (en) | 2003-11-12 | 2009-03-17 | E. I. Du Pont De Nemours And Company | Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast |
| US7202356B2 (en) | 2003-11-14 | 2007-04-10 | E. I. Du Pont De Nemours And Company | Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast |
| CN103451246B (zh) | 2004-04-22 | 2018-02-16 | 联邦科学技术研究组织 | 用重组细胞合成长链多不饱和脂肪酸 |
| US7550651B2 (en) | 2004-06-25 | 2009-06-23 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| US7264949B2 (en) | 2004-09-15 | 2007-09-04 | E.I. Du Pont De Nemours And Company | Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast |
| US7550286B2 (en) | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| US7189559B2 (en) | 2004-11-04 | 2007-03-13 | E. I. Du Pont De Nemours And Company | Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
| US7879591B2 (en) | 2004-11-04 | 2011-02-01 | E.I. Du Pont De Nemours And Company | High eicosapentaenoic acid producing strains of Yarrowia lipolytica |
| US20060094102A1 (en) | 2004-11-04 | 2006-05-04 | Zhixiong Xue | Ammonium transporter promoter for gene expression in oleaginous yeast |
| US7470532B2 (en) | 2005-10-19 | 2008-12-30 | E.I. Du Pont De Nemours And Company | Mortierella alpina C16/18 fatty acid elongase |
| DK1951866T3 (da) | 2005-11-23 | 2014-10-27 | Du Pont | Delta-9-elongaser og anvendelse heraf til fremstilling af flerumættede fedtsyrer |
| US7943823B2 (en) | 2006-04-28 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| WO2007133425A2 (en) | 2006-05-01 | 2007-11-22 | E.I. Du Pont De Nemours And Company | Fungal delta12 desaturase and delta15 desaturase motifs |
| CA2663768A1 (en) | 2006-10-23 | 2008-05-29 | E. I. Du Pont De Nemours And Company | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
| CN101617040B (zh) | 2006-10-30 | 2012-07-04 | 纳幕尔杜邦公司 | △17去饱和酶及其在制备多不饱和脂肪酸中的用途 |
| US7709239B2 (en) | 2006-12-07 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids |
| US7732155B2 (en) | 2006-12-13 | 2010-06-08 | National Research Council Of Canada | Methods for identifying lysophosphatidylcholine acyltransferases |
| CA2679988A1 (en) | 2007-04-03 | 2008-10-16 | E. I. Du Pont De Nemours And Company | Multizymes and their use in making polyunsaturated fatty acids |
| US8188338B2 (en) | 2007-04-10 | 2012-05-29 | E. I. Du Pont De Nemours And Company | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
| US8119860B2 (en) | 2007-04-16 | 2012-02-21 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
| CN101970638B (zh) | 2007-10-03 | 2015-02-11 | 纳幕尔杜邦公司 | 用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株 |
| AU2009293160B2 (en) | 2008-09-19 | 2013-11-21 | E. I. Du Pont De Nemours And Company | Mutant delta5 desaturases and their use in making polyunsaturated fatty acids |
| CA2744716C (en) | 2008-12-18 | 2017-05-23 | E.I. Du Pont De Nemours And Company | Reducing byproduction of malonates in a fermentation process |
| US8524485B2 (en) | 2009-06-16 | 2013-09-03 | E I Du Pont De Nemours And Company | Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases |
| EP2553107A2 (en) | 2010-03-31 | 2013-02-06 | E.I. Du Pont De Nemours And Company | Pentose phosphate pathway upregulation to increase production of non-native products of interest in transgenic microorganisms |
| WO2012027689A1 (en) | 2010-08-26 | 2012-03-01 | E. I. Du Pont De Nemours And Company | Recombinant microbial host cells for high eicosapentaenoic acid production |
| DK2609201T3 (en) | 2010-08-26 | 2016-06-06 | Du Pont | Mutant DELTA-9 elongases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids |
| US8969049B2 (en) | 2011-03-31 | 2015-03-03 | E I Du Pont De Nemours And Company | Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast |
| EP2694665A1 (en) | 2011-04-01 | 2014-02-12 | E. I. Du Pont de Nemours and Company | Yarrowia esterase/lipase promoter regions for gene expression in yeast |
| EP2694657A1 (en) | 2011-04-05 | 2014-02-12 | E. I. Du Pont de Nemours and Company | Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast |
| US8609369B2 (en) | 2011-04-07 | 2013-12-17 | E I Du Pont De Nemours And Company | Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast |
| WO2012162368A1 (en) | 2011-05-26 | 2012-11-29 | E. I. Du Pont De Nemours And Company | Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein |
-
2013
- 2013-12-18 EP EP13814811.9A patent/EP2935591B1/en not_active Not-in-force
- 2013-12-18 JP JP2015549593A patent/JP2016502851A/ja active Pending
- 2013-12-18 CA CA2892516A patent/CA2892516A1/en not_active Abandoned
- 2013-12-18 US US14/132,051 patent/US9005940B2/en not_active Expired - Fee Related
- 2013-12-18 BR BR112015014844A patent/BR112015014844A2/pt not_active Application Discontinuation
- 2013-12-18 WO PCT/US2013/075895 patent/WO2014100061A1/en active Application Filing
-
2015
- 2015-03-17 US US14/660,229 patent/US9441209B2/en active Active
- 2015-06-16 CL CL2015001674A patent/CL2015001674A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20140220645A1 (en) | 2014-08-07 |
| US9441209B2 (en) | 2016-09-13 |
| WO2014100061A1 (en) | 2014-06-26 |
| JP2016502851A (ja) | 2016-02-01 |
| CA2892516A1 (en) | 2014-06-26 |
| US20150218526A1 (en) | 2015-08-06 |
| EP2935591B1 (en) | 2017-07-05 |
| EP2935591A1 (en) | 2015-10-28 |
| BR112015014844A2 (pt) | 2019-01-15 |
| US9005940B2 (en) | 2015-04-14 |
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