CH647674A5 - HAIR COMPOSITION FOR THE TREATMENT OF HAIR BASED ON STIGMASTEROL AND LYOPHILIZED PURIFIED AMNIOTIC LIQUID. - Google Patents

Description

The present invention relates to a new hair cosmetic composition for the treatment of hair, in particular in order to reduce the fall and to promote its growth.

It is well known that the problem of hair loss, especially in men, is a relatively common phenomenon, especially at a certain period of life, and results from multiple causes that are still poorly defined and among which there is no doubt. that hereditary heritage plays a significant role.

The fact remains that hair loss can also be caused by certain other factors, in particular psychic.

Furthermore, a malfunction of the sebaceous glands can also lead to seborrheic alopecia, the outcome of which can be baldness.

In order to remedy as far as possible excessive hair loss and also to promote growth, numerous compositions have already been proposed, called tonics, based on active compounds of varied origin.

The characteristics which such hair compositions for hair treatment must have are essentially:

1. a slightly irritating action so as to promote blood circulation at the level of the dermal papillae,

2. the presence of vitamins or sulfur amino acids to improve the biosynthesis of keratin, and

3. the presence of antiseborrhoeic agents or certain drugs to control seborrhea and other phenomena of the scalp which can interfere with the growth or regrowth of hair.

Among the various active compounds used in the hair compositions, mention may in particular be made of certain phenols such as resorcinol and β-naphthol. In addition, certain natural extracts have also been recommended such as quinine, cade oil, capsicum, pylocarpine as well as certain sulfur amino acid derivatives such as those of cysteine or cysteamine.

Finally, it is important to mention the use of lyophilized amniotic fluid from a ruminant animal, preferably from a bovine, at a certain stage of gestation greater than 60 d but less than 90 d. This type of composition has been described in the US application of the applicant company No. 3733402 (French patents Nos 985458 and 22503) and has been shown to be particularly effective in particular in the case of seborrheic alopecia.

The present invention is in the same cosmetic field and relates to a composition making it possible to influence pilogenetic phenomena by inhibiting hair loss and by promoting their growth.

In other words, it is not the object of the present invention to treat subjects already suffering from baldness, but quite the contrary to treat those with certain warning signs, so as to stop the process or at least to slow down the effects.

The subject of the present invention is a hair composition in the form of a lotion intended to combat hair loss and to promote its growth, this composition containing, in solution in alcohol having an alcoholic degree> 98 °: i) stigmasterol in pure form or in the form of an unsaponifiable material of wheat germ oil or soybean oil, and ii) lyophilized amniotic liquid purified by alcohol solubilization.

The tests which have been carried out using such a composition have made it possible first to show its excellent activity in the Wistar rat, then the results observed have been confirmed in humans.

The hair growth in the Wistar rat could be monitored daily by a simple and direct method.

For each experiment, male Wistar rats, all born on the same day, are distributed in cages of five rats each and identified by a mark using picric acid. Four cages, or twenty animals, constitute a series. At the start of the experiment, the surface of the back of each animal is mowed for about 20 cm2 using an electric clipper.

3 d later, the first daily topical application of 0.5 ml of the composition to be tested is started for the treated series and distilled water for the control series.

The treatment is continued for three weeks, and a clinical scoring system makes it possible to follow the individual progress of the coat and to assess the effectiveness of the treatment. The second clinical reading notably allows the selection, in each series, of the five animals whose regrowth is starting to be visible.

These animals then receive an oxidation dye on the treated area. Daily topical applications are continued and a tuft of hair is removed daily.

These are then examined using a micrometric magnifying glass graduated in Vio mm, so as to measure the distance between the lower end of the bulb and the birth of the coloring. The measurement relates daily to 10 hairs for each animal.

By this method, it is thus possible to determine, by comparison of the treated animals and of the control animals, the variation of the average growth index.

The different results found by this method will be given below.

Stigmasterol or 3- (P) -hydroxy-24-ethyl-A5-22 cholestadiene (C29H480; molecular weight 412) is, with sitosterol, one of the constituents of phytosterols, high molecular weight alcohols present in many plants .

Stigmasterol as a monohydrate has a melting point of 170 ° C.

Stigmasterol can be obtained either by synthesis or, more commonly, from the mixture of phytosterols from certain plants particularly rich in this substance such as wheat germ and soybeans.

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The unsaponifiables of wheat germ oil and soybean oil which can be used in the compositions according to the invention are of known preparation and their composition could be determined with precision according to their origin; thus, the unsaponifiable matter of wheat germ oil contains from 9 to 13% of stigmasterol.

Preferably used, according to the invention, unsaponifiables of wheat germ or soybean oil having a stigmasterol content greater than or equal to 9%.

The stigmasterol concentration of the compositions according to the invention is generally between 0.1 and 0.5% by weight relative to the total weight of the composition.

The other active ingredient in the alcohols according to the invention is lyophilized amniotic liquid which has been purified beforehand by so-lubrication with alcohol.

As indicated above, it has already been recommended to use lyophilized amniotic liquid in the treatment of hair; however, in this case it was mainly aqueous solutions in which the amniotic fluid was soluble.

In order to obtain a fraction of amniotic liquid soluble in alcohol, the lyophilized amniotic liquid obtained according to the technique described in US Pat. No. 3,733,402 is treated with an alcohol, preferably ethanol, which makes it possible to dissolve it. about 50%.

The checks carried out, in particular by chromatography on Se-phadex LH20, made it possible to show that only the various active fractions of the lyophilized amniotic liquid were present in the alcoholic solution.

Such purification by solubilization has made it possible to improve, on the one hand, the activity and, on the other hand, the penetration by elimination of inhibitory substances such as proteins with high molecular weight.

The elimination of inactive substances also makes it possible to avoid certain reactions of an immunological nature.

Since, according to the invention, the vehicle for the hair composition is alcohol, ethanol or isopropanol, but preferably ethanol, it is not necessary to isolate the purified product, the alcoholic solution resulting from the process of purification can be used directly.

In this case, an amount of alcoholic solution is used such that the hair composition according to the invention contains from 0.3 to 2% and preferably from 0.4 to 0.6% by weight of purified amniotic liquid.

The hair compositions according to the invention can also contain various other ingredients, and in particular a perfume, a coloring substance capable of tinting the composition, a resin or a treating agent.

The compositions according to the invention have been found to exhibit excellent stability over time, both from the bacteriological point of view and from its original activity.

The results obtained on hair growth in the Wistar rat after the third week of treatment and according to the technique described above have been collected below.

C Table at the top of the next column)

The results observed make it possible to show that, by combining stigmasterol with purified amniotic fluid, there is obtained, in terms of hair growth, a very marked improvement compared to that observed when these substances are used alone.

The present invention also relates to a method of treating the hair in order to reduce the fall and to promote its growth, consisting in applying a sufficient amount of the composition according to the invention as described above and in massing scalp.

In general, an amount of 8 to 60 cm 3 is sufficient to permeate the entire scalp and hair.

The treatment should preferably be applied daily for an extended period of the order of 1 to 3 months, the applications then being able to be spaced.

According to a preferred form of treatment according to the invention, the com

Product

Concentration (%)

Vehicle

Change in average growth index compared to control (%)

Pure Stigmasterol (I)

0.2

Absolute ethanol

+46

Unsaponifiable with wheat germ oil at 10% stigmasterol

(T)

2

Absolute ethanol

+39

Purified amniotic fluid (II)

0.5

Absolute ethanol

+47

(I) + (H)

(I) 0.2

(II) 0.5

Absolute ethanol

+56

position is diluted at the time of use with water or an aqueous solution containing a treatment product such as a cys-tein derivative, in particular S-carboxymethylcysteine, or collagen, then applied to the scalp. This avoids certain irritation phenomena on subjects particularly sensitive to pure alcohol.

We will now give by way of illustration, and without any limiting character, several examples of hair composition according to the invention.

Example 1:

A hair composition for the treatment of hair is prepared according to the invention in order to stop their loss and promote their growth by mixing the following ingredients:

0.2g pure stigmasterol

Perfume 0.1 g Purified amniotic liquid (0.5% solution

in absolute ethanol) q.s.p. 100g

This composition (30 cm3), applied by friction to a subject with abundant hair loss, made it possible, after a daily treatment of 2 months, to stop the loss. There was also a marked improvement in hair growth compared to that observed before the start of treatment.

Example 2:

Stigmasterol pure 0.3 g

Perfume 0.05 g

Vinyl acetate / crotonic acid (90/10) copolymer 0.3 g

Purified amniotic liquid (0.4% solution in absolute ethanol) q.s.p. 100g

25 cm3 of the above composition are diluted with 20 cm3 of water containing a treating product (0.2% S-carboxymethylcysteine). Daily treatment for approximately 3 months has given remarkable results on various subjects with abundant hair loss, the daily fall after treatment being on average that of normal subjects.

Comparable results were obtained using the following composition with or without dilution using an aqueous solution.

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Example 3:

Pure stigmasterol

Vinylpyrrolidone quaternary copolymer sold under the name of Gafquat 734 Perfume

Colorant (sufficient to tint)

Purified amniotic liquid (0.5% solution in absolute ethanol)

Example 4:

Pure Stigmasterol Perfume

Colorant (sufficient to tint)

Camphor

0.5 g

0.6 g 0.1g q.s.p. 100g

0.4 g 0.08 g

0.05 g

Purified amniotic liquid (0.4% solution in absolute ethanol) q.s.p. 100g

Before being applied to the hair, the above composition (20 cm3) is diluted with 20 cm3 of water. Daily treatment for 2 months on a subject with abundant hair loss has produced excellent results, the daily loss corresponding to that of a normal subject.

Example 5:

Unsaponifiable with wheat germ oil containing 10% stigmasterol 2 g

Perfume 0.1 g

Purified amniotic liquid (0.4% solution in absolute ethanol) q.s.p. 100g

R

Claims (9)

647,674 1. Hair composition in the form of a lotion intended to combat hair loss and to promote its growth, characterized in that it contains in solution in alcohol having an alcoholic degree ^ 98 °: i) stigmasterol in pure form or in the form of an unsaponifiable material of wheat germ oil or soybean oil, and ii) freeze-dried amniotic liquid purified by alcohol solubilization. 2. Composition according to claim 1, characterized in that the unsaponifiable matter of wheat germ oil or soybean oil has a stigmasterol content greater than or equal to 9%. 2 3. Composition according to one of claims 1 or 2, characterized in that the concentration of stigmasterol is between 0.1 and 0.5% by weight relative to the total weight of the composition. 4. Composition according to claim 1, characterized in that the alcohol is ethanol or isopropanol. 5. Composition according to claim 1, characterized in that the lyophilized amniotic liquid comes from a ruminant animal and was collected at a gestation stage greater than 60 d but less than 90 d. 6. Composition according to one of the preceding claims, characterized in that it contains 0.3 to 2% and preferably 0.4 to 0.6% by weight of the purified amniotic liquid. 7. Composition according to one of the preceding claims, characterized in that it contains a perfume, a coloring substance for tinting the composition, a resin or a treating agent. 8. A method of treating hair with a view to reducing the fall and promoting its growth, characterized in that it consists in applying a sufficient amount of a composition according to one of claims 1 to 7 and to massage the scalp. 9. Method according to claim 8, characterized in that the composition is diluted, with water or an aqueous solution containing a treating product, before its application to the scalp.