CA3234004A1 - Microfluidic devices and methods for use thereof in multicellular assays of secretion - Google Patents

Microfluidic devices and methods for use thereof in multicellular assays of secretion Download PDF

Info

Publication number
CA3234004A1
CA3234004A1 CA3234004A CA3234004A CA3234004A1 CA 3234004 A1 CA3234004 A1 CA 3234004A1 CA 3234004 A CA3234004 A CA 3234004A CA 3234004 A CA3234004 A CA 3234004A CA 3234004 A1 CA3234004 A1 CA 3234004A1
Authority
CA
Canada
Prior art keywords
receptor
cell
cells
readout
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3234004A
Other languages
English (en)
French (fr)
Inventor
Marketa RICICOVA
Kevin Albert HEYRIES
Hans Zahn
Oleh Petriv
Veronique Lecault
Anupam Singhal
Daniel J. Da Costa
Carl L. G. Hansen
Brad Nelson
Julie NIELSEN
Kathleen LISAINGO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of British Columbia
Original Assignee
University of British Columbia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of British Columbia filed Critical University of British Columbia
Publication of CA3234004A1 publication Critical patent/CA3234004A1/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1018—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Fluid Mechanics (AREA)
  • Communicable Diseases (AREA)
  • Pulmonology (AREA)
  • Robotics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3234004A 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion Pending CA3234004A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361806329P 2013-03-28 2013-03-28
US61/806,329 2013-03-28
CA2906231A CA2906231C (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2906231A Division CA2906231C (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Publications (1)

Publication Number Publication Date
CA3234004A1 true CA3234004A1 (en) 2014-10-02

Family

ID=51622310

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3234004A Pending CA3234004A1 (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion
CA2906231A Active CA2906231C (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2906231A Active CA2906231C (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Country Status (9)

Country Link
US (3) US10725024B2 (enExample)
EP (2) EP2978855B1 (enExample)
JP (4) JP6608803B2 (enExample)
KR (3) KR102257305B1 (enExample)
CN (3) CN109342710A (enExample)
AU (3) AU2014245806B2 (enExample)
CA (2) CA3234004A1 (enExample)
NZ (1) NZ711723A (enExample)
WO (1) WO2014153651A1 (enExample)

Families Citing this family (125)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691145B2 (en) 2009-11-16 2014-04-08 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for water purification
US9783775B2 (en) 2012-03-15 2017-10-10 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9272234B2 (en) 2012-03-15 2016-03-01 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US10370635B2 (en) 2012-03-15 2019-08-06 Flodesign Sonics, Inc. Acoustic separation of T cells
US10953436B2 (en) 2012-03-15 2021-03-23 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US9745548B2 (en) 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US10322949B2 (en) 2012-03-15 2019-06-18 Flodesign Sonics, Inc. Transducer and reflector configurations for an acoustophoretic device
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9752114B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc Bioreactor using acoustic standing waves
US9567559B2 (en) 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9796956B2 (en) 2013-11-06 2017-10-24 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US10689609B2 (en) 2012-03-15 2020-06-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9752113B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc. Acoustic perfusion devices
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US10737953B2 (en) 2012-04-20 2020-08-11 Flodesign Sonics, Inc. Acoustophoretic method for use in bioreactors
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9745569B2 (en) 2013-09-13 2017-08-29 Flodesign Sonics, Inc. System for generating high concentration factors for low cell density suspensions
CA2927701C (en) 2013-10-22 2020-10-13 Berkeley Lights, Inc. Microfluidic devices having sequestration pens and methods of testing biological micro-objects with same
EP3783094B1 (en) 2013-10-22 2023-10-11 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
US11318479B2 (en) 2013-12-18 2022-05-03 Berkeley Lights, Inc. Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
CN105980058A (zh) 2014-01-07 2016-09-28 达克雷诊断器材有限公司 流体输送装置、系统和方法
CA2935960C (en) 2014-01-08 2023-01-10 Bart Lipkens Acoustophoresis device with dual acoustophoretic chamber
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
EP3146079B1 (en) 2014-05-22 2019-01-30 The University Of British Columbia Methods for determining lymphocyte receptor chain pairs
US20150352547A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating Microfluidic Structures and Trapping Bubbles
US9744483B2 (en) 2014-07-02 2017-08-29 Flodesign Sonics, Inc. Large scale acoustic separation device
US20160123975A1 (en) * 2014-11-03 2016-05-05 Daktari Diagnostics, Inc. Mesh Microfluidic Mixing Chamber
WO2016090148A1 (en) 2014-12-03 2016-06-09 IsoPlexis Corporation Analysis and screening of cell secretion profiles
CN107249742B (zh) 2014-12-08 2019-12-06 伯克利之光生命科技公司 微流体装置中定向流动的致动微流体结构及使用它的方法
EP3230718B1 (en) 2014-12-09 2022-03-02 Berkeley Lights, Inc. Automated detection of assay-positive areas or of analyte quantities in microfluidic devices
CA2970149C (en) 2014-12-10 2022-12-13 Berkeley Lights, Inc. Systems for operating electrokinetic devices
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
US11353448B2 (en) * 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
US10876156B2 (en) 2015-03-13 2020-12-29 President And Fellows Of Harvard College Determination of cells using amplification
US10106770B2 (en) 2015-03-24 2018-10-23 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
US10590461B2 (en) * 2015-04-21 2020-03-17 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
AU2016250689B2 (en) 2015-04-22 2021-07-08 Berkeley Lights, Inc. Microfluidic cell culture
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
US10973227B2 (en) 2015-04-22 2021-04-13 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US20170029802A1 (en) * 2015-07-28 2017-02-02 Flodesign Sonics, Inc. Acoustic affinity separation
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
AU2016361413B2 (en) 2015-11-23 2022-04-14 Berkeley Lights, Inc. In situ-generated microfluidic isolation structures, kits and methods of use thereof
CN108603878A (zh) 2015-12-08 2018-09-28 伯克利之光生命科技公司 微流体设备和试剂盒及其使用方法
EP3394326B1 (en) * 2015-12-21 2022-09-14 The University Of British Columbia Highly parallel assays for simultaneous identification of antibody sequences and binding partners
KR20230042540A (ko) 2015-12-30 2023-03-28 버클리 라잇츠, 인크. 광학적으로 구동되는 대류 및 변위를 위한 미세유체 디바이스들, 키트들 및 그 방법들
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
CN108778508B (zh) * 2016-01-14 2022-09-13 欧洲分子生物学实验室 配体诱导的细胞表达的微流体分析
US10712344B2 (en) 2016-01-15 2020-07-14 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
EP3411710A1 (en) 2016-02-05 2018-12-12 The Broad Institute Inc. Multi-stage, multiplexed target isolation and processing from heterogeneous populations
EP3429753A4 (en) 2016-03-16 2019-11-06 Berkeley Lights, Inc. METHODS, SYSTEMS AND DEVICES FOR SELECTION AND PRODUCTION OF GENERICALLY WORKED CLONES
WO2017156634A1 (en) * 2016-03-17 2017-09-21 The University Of British Columbia Devices and methods for cellular secretion analysis
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
AU2017248822B2 (en) 2016-04-15 2022-09-15 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
WO2017184998A1 (en) * 2016-04-22 2017-10-26 Arizona Board Of Regents On Behalf Of Arizona State University A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation
US10710006B2 (en) 2016-04-25 2020-07-14 Flodesign Sonics, Inc. Piezoelectric transducer for generation of an acoustic standing wave
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
CN114891635A (zh) 2016-05-03 2022-08-12 弗洛设计声能学公司 利用声泳的治疗细胞洗涤、浓缩和分离
AU2017271673B2 (en) 2016-05-26 2022-04-14 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use
JP2019520825A (ja) * 2016-06-30 2019-07-25 ジェネラル オートメーション ラボ テクノロジーズ インコーポレイテッド 磁性粒子を用いたハイスループット微生物学適用高分解能システム、キット、装置および方法
AU2017298545B2 (en) 2016-07-21 2022-10-27 Berkeley Lights, Inc. Sorting of T lymphocytes in a microfluidic device
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
US10858649B2 (en) 2016-09-15 2020-12-08 Augmenta Bioworks, Inc. Immune repertoire sequence amplification methods and applications
CN110494543A (zh) 2016-10-19 2019-11-22 弗洛设计声能学公司 通过声学的亲和细胞提取
CN106520537B (zh) * 2016-10-21 2018-10-23 南通大学 一种t细胞免疫应答的微流控光学分析系统及分析方法
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11402308B2 (en) 2016-12-06 2022-08-02 Brandeis University Freezable fluid cell for cryo-electron microscopy
CA3048645A1 (en) * 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
JP2020528747A (ja) 2017-07-21 2020-10-01 バークレー ライツ，インコーポレイテッド 抗原提示合成表面、共有結合官能化表面、活性化ｔ細胞及びそれらの使用
CN111246941B (zh) * 2017-10-02 2022-01-04 哈利法科学技术大学 模仿淋巴结的解剖和生理结构的微流体装置及芯片实验室
JP7209701B2 (ja) * 2017-10-03 2023-01-20 アベイルズ メディカル，インコーポレイテッド レドックス反応に基づいて微生物の濃度及び抗感染剤に対する微生物の感受性を決定する装置、システム、及び方法
WO2019075476A2 (en) 2017-10-15 2019-04-18 Berkeley Lights, Inc. METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS
WO2019079725A1 (en) 2017-10-19 2019-04-25 Georgia Tech Research Corporation MESOFLUIDIC DEVICE FOR CULTIVATION OF CELLULAR AGGREGATES
KR20220066413A (ko) 2017-12-14 2022-05-24 프로디자인 소닉스, 인크. 음향 트랜스듀서 구동기 및 제어기
CN111788483B (zh) * 2018-03-12 2024-12-27 索尼公司 细胞评价设备和细胞评价系统
JP7497293B2 (ja) 2018-03-16 2024-06-10 ゾエティス・サービシーズ・エルエルシー インターロイキン－３１に対するペプチドワクチン
KR102828306B1 (ko) 2018-03-16 2025-07-01 조에티스 서비시즈 엘엘씨 수의학적 용도를 위한 인터류킨-31 단클론성 항체
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
BR112020020083A2 (pt) * 2018-03-30 2021-01-05 Exosomics S.P.A Uso in vitro de fibras ocas, método in vitro para obtenção de sangue ou de um derivado de sangue e método in vitro para analisar o teor de vesículas extracelulares de um sangue ou amostra líquida derivada de sangue
WO2019195795A1 (en) * 2018-04-05 2019-10-10 University Of Virginia Patent Foundation 3d spatially patterned lymph node on a microfluidic device
US11666914B2 (en) * 2018-05-09 2023-06-06 Tecan Trading Ag Cartridge, electrowetting sample processing system and bead manipulation method
CN110499251A (zh) * 2018-05-17 2019-11-26 中国科学院大连化学物理研究所 高通量多参数的单细胞源性细胞外囊泡分析芯片及应用
EP3849705A4 (en) * 2018-09-14 2022-06-01 Berkeley Lights, Inc. Methods for assaying binding affinity
WO2020061499A1 (en) 2018-09-21 2020-03-26 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
US11662341B2 (en) 2018-10-10 2023-05-30 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
AU2019358198A1 (en) 2018-10-11 2021-05-27 Berkeley Lights, Inc. Systems and methods for identification of optimized protein production and kits therefor
CN109576345A (zh) * 2018-10-17 2019-04-05 西人马（厦门）科技有限公司 一种用于dna提取的微流控芯片及其检测方法
US20200139362A1 (en) 2018-10-18 2020-05-07 Berkeley Lights, Inc. Proto-Antigen-Presenting Synthetic Surfaces, Activated T Cells, and Uses Thereof
KR20210089193A (ko) 2018-11-01 2021-07-15 버클리 라잇츠, 인크. 미세유체 디바이스에서 생물학적 세포를 분석하기 위한 방법
GB201820617D0 (en) * 2018-12-18 2019-01-30 Greindx As System for capturing cells
EP3670667A1 (en) * 2018-12-19 2020-06-24 Paris Sciences et Lettres - Quartier Latin Identification of cognate pairs of ligands and receptors
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
US20200300861A1 (en) * 2019-03-22 2020-09-24 Augmenta Bioworks, Inc. Isolation of Single Cells and Uses Thereof
CN110006866B (zh) * 2019-04-16 2022-04-22 杭州迈尔德生物科技有限公司 一种阿片类活性物质的通用检测方法及其检测试剂盒
CN114126762B (zh) 2019-04-30 2023-01-03 伯克利之光生命科技公司 用于包封和测定细胞的方法
MX2021014439A (es) 2019-05-28 2022-05-19 Xilis Inc Metodos y aparatos para microorganoesferas derivadas de pacientes.
US20200392578A1 (en) * 2019-06-14 2020-12-17 The Regents Of The University Of California Methods of sequencing antibody chains from hybridomas and kits for practicing same
KR20220070220A (ko) * 2019-09-30 2022-05-30 도오꾜오까고오교 가부시끼가이샤 분비물 산생 세포의 스크리닝 방법, 및 분비물 산생 세포의 스크리닝 키트
WO2021097456A1 (en) * 2019-11-17 2021-05-20 The Regents Of The University Of California Guided Microfluidic Flow for Cell Capture, Indexing, and Directed Release
CA3158045A1 (en) * 2019-11-21 2021-05-27 Emulate Inc. Antibody producing microfluidic devices
CN112557475B (zh) * 2020-02-19 2021-07-06 南京启医科技有限公司 一种基于微流控和elisa分析的外泌体分离检测系统
CN113373104B (zh) * 2020-03-09 2022-11-18 浙江大学 一种用于稀有细胞高纯度分选的装置及方法
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
CN113529075B (zh) * 2020-04-20 2022-05-03 厦门大学 一种液态金属复合多孔膜及其制备方法和应用
US20230182135A1 (en) * 2020-05-14 2023-06-15 Bifrost Biosystems A microfluidic device having specifically designed detection chambers
US11725052B2 (en) 2020-08-18 2023-08-15 Cephalon Llc Anti-PAR-2 antibodies and methods of use thereof
TWI729914B (zh) * 2020-08-18 2021-06-01 國立臺灣海洋大學 鮮度指示裝置
NL2026383B1 (en) * 2020-08-31 2022-04-29 Lumicks Ca Holding B V Method and system for studying objects, in particular biological cells
EP4337260A4 (en) * 2021-05-14 2025-01-29 The Texas A&M University System PLATFORM FOR FAST, LABEL-FREE, AUTOMATED ASSESSMENT OF STERILITY AND BIOCONTAMINATION
US20250290932A1 (en) * 2022-05-19 2025-09-18 Triplebar Bio, Inc. Multiparametric discovery and optimization platform
CN115735846B (zh) * 2022-11-29 2025-09-16 复旦大学附属华山医院 磁场联合光化学诱导无创性缺血性脑卒中模型的建立方法
WO2024130037A1 (en) * 2022-12-14 2024-06-20 President And Fellows Of Harvard College Systems and methods for identifying gpcr modulators and other agents
KR102543112B1 (ko) * 2023-04-12 2023-06-13 주식회사 브레디스헬스케어 유체 시료 내에 극미량으로 존재하는 분석 대상 물질의 입자를 검출하기 위한 방법
CN116626293A (zh) * 2023-06-08 2023-08-22 复旦大学 一种用于检测溶组织内阿米巴感染的微流控芯片
WO2025111584A1 (en) * 2023-11-22 2025-05-30 Georgia Tech Research Corporation An electronic microfluidic platform for on-chip apoptosis quantification using annexin v-based ps externalization detection
CN118731352B (zh) * 2024-09-02 2024-12-06 天津锐尔康生物科技有限公司 用于细胞表面标志物的自动化分析检测方法及系统

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008010A (en) * 1996-11-01 1999-12-28 University Of Pittsburgh Method and apparatus for holding cells
DE19725190A1 (de) * 1997-06-14 1998-12-17 Innova Gmbh Vorrichtungen mit integrierten Elektroden aus elektrisch leitfähigen Kunststoffen
US6740497B2 (en) * 1998-03-06 2004-05-25 The Regents Of The University Of California Method and apparatus for detecting cancerous cells using molecules that change electrophoretic mobility
JP2006513702A (ja) 2002-09-09 2006-04-27 ヌラ インコーポレーティッド Ｇタンパク質共役受容体およびその使用
KR101243577B1 (ko) * 2004-10-07 2013-03-20 아고스 쎄라퓨틱스, 인코포레이티드 성숙 수지상 세포 조성물 및 그의 배양 방법
US20080264863A1 (en) 2004-12-03 2008-10-30 California Institute Of Technology Microfluidic Sieve Valves
US8796020B2 (en) * 2005-06-17 2014-08-05 Inha-Industry Partnership Institute Manufacturing process for fresh and frozen stem cells
WO2008143910A2 (en) * 2007-05-18 2008-11-27 University Of Massachusetts A strategy for cloning and expressing the extracellular domains of receptors as soluble proteins
DK2271657T3 (en) * 2008-03-04 2017-05-15 Crystal Bioscience Inc GEL-MICRO-DROP COMPOSITION AND METHOD OF USING IT
US20110275063A1 (en) * 2008-07-11 2011-11-10 President And Fellows Of Harvard College Systems and methods of droplet-based selection
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) * 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
CN103392124B (zh) 2010-12-03 2016-04-20 塞普莱有限公司 细胞功能的微分析
CN116179323A (zh) 2011-05-27 2023-05-30 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
US9250229B2 (en) * 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis

Also Published As

Publication number Publication date
AU2014245806A9 (en) 2016-06-16
AU2022202333A1 (en) 2022-04-28
JP2016515823A (ja) 2016-06-02
WO2014153651A1 (en) 2014-10-02
KR102668221B1 (ko) 2024-05-23
NZ711723A (en) 2021-12-24
JP7374936B2 (ja) 2023-11-07
AU2014245806B2 (en) 2019-10-31
JP2021078503A (ja) 2021-05-27
WO2014153651A9 (en) 2016-03-10
CN109342710A (zh) 2019-02-15
CA2906231A1 (en) 2014-10-02
KR102257305B1 (ko) 2021-05-28
CN105492621A (zh) 2016-04-13
US20160252495A1 (en) 2016-09-01
JP7699639B2 (ja) 2025-06-27
JP6825065B2 (ja) 2021-02-03
EP2978855A4 (en) 2016-10-26
WO2014153651A8 (en) 2014-11-06
KR20220070579A (ko) 2022-05-31
AU2020200414B2 (en) 2022-04-14
KR20150134429A (ko) 2015-12-01
CA2906231C (en) 2024-05-14
US20200363401A1 (en) 2020-11-19
JP2024020238A (ja) 2024-02-14
AU2014245806A1 (en) 2015-09-24
EP2978855A1 (en) 2016-02-03
JP2020043856A (ja) 2020-03-26
KR20210063450A (ko) 2021-06-01
AU2022202333B2 (en) 2024-12-19
US20220365072A1 (en) 2022-11-17
EP2978855B1 (en) 2021-01-13
AU2020200414A1 (en) 2020-02-13
KR102402451B1 (ko) 2022-05-26
EP3865212A1 (en) 2021-08-18
US10725024B2 (en) 2020-07-28
EP3865212B1 (en) 2024-03-13
JP6608803B2 (ja) 2019-11-20
CN115074413A (zh) 2022-09-20

Similar Documents

Publication Publication Date Title
US20220365072A1 (en) Microfluidic Devices and Methods for Use Thereof in Multicellular Assays of Secretion
AU2023208186A1 (en) Devices and methods for cellular secretion analysis
HK40058018A (en) Microfluidic devices and methods for use thereof in multicellular assays of secretion

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20240402

EEER Examination request

Effective date: 20240402

EEER Examination request

Effective date: 20240402