CA3229003A1 - Preparation of libraries of protein variants expressed in eukaryotic cells - Google Patents
Preparation of libraries of protein variants expressed in eukaryotic cells Download PDFInfo
- Publication number
- CA3229003A1 CA3229003A1 CA3229003A CA3229003A CA3229003A1 CA 3229003 A1 CA3229003 A1 CA 3229003A1 CA 3229003 A CA3229003 A CA 3229003A CA 3229003 A CA3229003 A CA 3229003A CA 3229003 A1 CA3229003 A1 CA 3229003A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- gene
- dna
- sequence
- library
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP21193102 | 2021-08-25 | ||
| EP21193102.7 | 2021-08-25 | ||
| PCT/EP2022/073549 WO2023025834A1 (en) | 2021-08-25 | 2022-08-24 | Preparation of libraries of protein variants expressed in eukaryotic cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3229003A1 true CA3229003A1 (en) | 2023-03-02 |
Family
ID=77774665
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3229003A Pending CA3229003A1 (en) | 2021-08-25 | 2022-08-24 | Preparation of libraries of protein variants expressed in eukaryotic cells |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20250129362A1 (https=) |
| EP (1) | EP4392571A1 (https=) |
| JP (1) | JP2024531431A (https=) |
| CN (1) | CN118525099A (https=) |
| AU (1) | AU2022334794A1 (https=) |
| CA (1) | CA3229003A1 (https=) |
| WO (1) | WO2023025834A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024251820A1 (en) | 2023-06-06 | 2024-12-12 | Iontas Limited | Preparation of libraries of bispecific binders expressed in eukaryotic cells |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61134325A (ja) | 1984-12-04 | 1986-06-21 | Teijin Ltd | ハイブリツド抗体遺伝子の発現方法 |
| GB8607679D0 (en) | 1986-03-27 | 1986-04-30 | Winter G P | Recombinant dna product |
| ES2156149T3 (es) | 1992-12-04 | 2001-06-16 | Medical Res Council | Proteinas de union multivalente y multiespecificas, su fabricacion y su uso. |
| DK1137941T4 (da) | 1998-12-10 | 2014-01-06 | Brystol Myers Squibb Company | Protein-scaffolds til antistof-mimetika og andre bindingsproteiner |
| CA2789446A1 (en) | 2010-02-12 | 2011-08-18 | Oncomed Pharmaceuticals, Inc. | Methods for identifying and isolating cells expressing a polypeptide |
| AU2012249390B2 (en) | 2011-04-27 | 2017-03-30 | Amyris, Inc. | Methods for genomic modification |
| BR112015031639A2 (pt) * | 2013-06-19 | 2019-09-03 | Sigma Aldrich Co Llc | integração alvo |
| US9209965B2 (en) | 2014-01-14 | 2015-12-08 | Microsemi Semiconductor Ulc | Network interface with clock recovery module on line card |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| US11293033B2 (en) * | 2016-05-18 | 2022-04-05 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| GB201720351D0 (en) | 2017-12-06 | 2018-01-17 | Iontas Ltd | Selecting for developability in drug discovery |
| IL275462B2 (en) * | 2017-12-22 | 2026-01-01 | Genentech Inc | Targeted integration of nucleic acids |
-
2022
- 2022-08-24 EP EP22769156.5A patent/EP4392571A1/en active Pending
- 2022-08-24 AU AU2022334794A patent/AU2022334794A1/en active Pending
- 2022-08-24 CA CA3229003A patent/CA3229003A1/en active Pending
- 2022-08-24 US US18/683,728 patent/US20250129362A1/en active Pending
- 2022-08-24 CN CN202280064645.4A patent/CN118525099A/zh active Pending
- 2022-08-24 WO PCT/EP2022/073549 patent/WO2023025834A1/en not_active Ceased
- 2022-08-24 JP JP2024510636A patent/JP2024531431A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4392571A1 (en) | 2024-07-03 |
| AU2022334794A1 (en) | 2024-03-28 |
| JP2024531431A (ja) | 2024-08-29 |
| US20250129362A1 (en) | 2025-04-24 |
| CN118525099A (zh) | 2024-08-20 |
| WO2023025834A1 (en) | 2023-03-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD Year of fee payment: 3 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250729 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250729 |