CA3224352A1 - Compositions and methods for pairwise sequencing - Google Patents

Compositions and methods for pairwise sequencing Download PDF

Info

Publication number
CA3224352A1
CA3224352A1 CA3224352A CA3224352A CA3224352A1 CA 3224352 A1 CA3224352 A1 CA 3224352A1 CA 3224352 A CA3224352 A CA 3224352A CA 3224352 A CA3224352 A CA 3224352A CA 3224352 A1 CA3224352 A1 CA 3224352A1
Authority
CA
Canada
Prior art keywords
immobilized
sequencing
primer
molecules
concatemer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3224352A
Other languages
English (en)
French (fr)
Inventor
Michael Kim
Hua Yu
Yu-Hsien HWANG-FU
Marco Tjioe
Andrew Boddicker
Sinan ARSLAN
Junhua Zhao
Molly He
Samantha SNOW
William Light
Matthew KELLINGER
Michael Previte
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Element Biosciences Inc
Original Assignee
Element Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US17/377,285 external-priority patent/US11236388B1/en
Application filed by Element Biosciences Inc filed Critical Element Biosciences Inc
Priority claimed from PCT/US2022/034038 external-priority patent/WO2022266470A1/en
Publication of CA3224352A1 publication Critical patent/CA3224352A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3224352A 2021-06-17 2022-06-17 Compositions and methods for pairwise sequencing Pending CA3224352A1 (en)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US202163212059P 2021-06-17 2021-06-17
US63/212,059 2021-06-17
US17/377,285 2021-07-15
US17/377,285 US11236388B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/377,284 2021-07-15
US17/377,279 2021-07-15
US17/377,279 US11535892B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/377,283 2021-07-15
US17/377,284 US11220707B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/377,283 US11427855B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/521,239 2021-11-08
US17/521,239 US11891651B2 (en) 2021-06-17 2021-11-08 Compositions and methods for pairwise sequencing
US202117554396A 2021-12-17 2021-12-17
US17/554,396 2021-12-17
PCT/US2022/034038 WO2022266470A1 (en) 2021-06-17 2022-06-17 Compositions and methods for pairwise sequencing

Publications (1)

Publication Number Publication Date
CA3224352A1 true CA3224352A1 (en) 2022-12-22

Family

ID=89324679

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3224352A Pending CA3224352A1 (en) 2021-06-17 2022-06-17 Compositions and methods for pairwise sequencing

Country Status (7)

Country Link
EP (1) EP4355913A1 (https=)
JP (1) JP2024525180A (https=)
KR (1) KR20240047357A (https=)
AU (1) AU2022294092A1 (https=)
CA (1) CA3224352A1 (https=)
GB (1) GB2623234B (https=)
IL (1) IL309338A (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025163526A1 (en) * 2024-01-30 2025-08-07 Element Biosciences, Inc. Method for improving nucleic acid sequencing quality by eliminating nucleic acids with deaminated bases from library and method for sequencing in which complexes of primers, polymerases and labelled probes are bound to concatemers

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013019361A1 (en) * 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
US10364464B2 (en) * 2011-08-08 2019-07-30 The Broad Institute, Inc. Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
US11085073B2 (en) * 2015-04-24 2021-08-10 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
JP7587425B2 (ja) * 2018-12-18 2024-11-20 イルミナ ケンブリッジ リミテッド 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物
KR20240094019A (ko) * 2019-09-23 2024-06-24 엘리먼트 바이오사이언스, 인크. 세포적으로 처리가능한 핵산 서열분석 방법
WO2022015600A2 (en) * 2020-07-13 2022-01-20 Singular Genomics Systems, Inc. Methods of sequencing complementary polynucleotides
KR20230091116A (ko) * 2020-10-21 2023-06-22 일루미나, 인코포레이티드 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법
CA3196800A1 (en) * 2020-10-30 2022-05-05 Element Biosciences, Inc. Reagents for massively parallel nucleic acid sequencing

Also Published As

Publication number Publication date
JP2024525180A (ja) 2024-07-10
IL309338A (en) 2024-02-01
AU2022294092A1 (en) 2024-01-25
KR20240047357A (ko) 2024-04-12
GB2623234B (en) 2026-03-25
GB202400380D0 (en) 2024-02-28
EP4355913A1 (en) 2024-04-24
GB2623234A (en) 2024-04-10

Similar Documents

Publication Publication Date Title
US12421545B2 (en) Compositions and methods for preparing nucleic acid nanostructures using compaction oligonucleotides
US12606819B2 (en) PCR-free library preparation using double-stranded splint adaptors and methods of use
US11236388B1 (en) Compositions and methods for pairwise sequencing
US20250333787A1 (en) Methods and reagents for nucleic acid analysis
US12540350B2 (en) Spatially resolved surface capture of nucleic acids
WO2022266470A1 (en) Compositions and methods for pairwise sequencing
KR20240171199A (ko) 이중 가닥 스플린트 어댑터 및 사용 방법
WO2025024465A1 (en) On-support circularization and amplification for generating immobilized nucleic acid concatemer molecules
KR20250086817A (ko) 범용 긴 스플린트 가닥을 갖는 이중가닥 스플린트 어댑터 및 사용 방법
US20250019760A1 (en) Compositions and methods for sequencing multiple regions of a template molecule using enzyme-based reagents
KR20240055718A (ko) 조작된 폴리머라제
WO2025120579A1 (en) Compositions and methods for sequencing multiple regions of a template molecule using read-capping nucleotide analogs
CA3224352A1 (en) Compositions and methods for pairwise sequencing
WO2025191535A1 (en) Partially double-stranded splint adaptors and methods of use
WO2025240909A1 (en) Hybridization-based capture and enrichment of target nucleic acid sequences
HK40128283A (zh) 用於使用压实寡核苷酸制备核酸纳米结构的组合物和方法
HK40130192A (en) Reagents for massively parallel nucleic acid sequencing

Legal Events

Date Code Title Description
MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 2ND ANNIV.) - STANDARD

Year of fee payment: 2

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20240923

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20240923

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20240923

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD

Year of fee payment: 3

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250530

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250530