JP2024525180A - ペアワイズシーケンシングのための組成物及び方法 - Google Patents

ペアワイズシーケンシングのための組成物及び方法 Download PDF

Info

Publication number
JP2024525180A
JP2024525180A JP2023577938A JP2023577938A JP2024525180A JP 2024525180 A JP2024525180 A JP 2024525180A JP 2023577938 A JP2023577938 A JP 2023577938A JP 2023577938 A JP2023577938 A JP 2023577938A JP 2024525180 A JP2024525180 A JP 2024525180A
Authority
JP
Japan
Prior art keywords
immobilized
sequencing
primer
molecules
soluble
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023577938A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024525180A5 (https=
Inventor
アースラン シナン
チャオ チュンホア
ヒー モリー
スノー サマンサ
ライト ウィリアム
ケリンガー マシュー
プリバイト マイケル
キム マイケル
ユイ ホア
ファン-フー ユイ-シエン
ジョエ マルコ
ボディッカー アンドリュー
Original Assignee
エレメント バイオサイエンシズ,インコーポレイティド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US17/377,285 external-priority patent/US11236388B1/en
Application filed by エレメント バイオサイエンシズ,インコーポレイティド filed Critical エレメント バイオサイエンシズ,インコーポレイティド
Priority claimed from PCT/US2022/034038 external-priority patent/WO2022266470A1/en
Publication of JP2024525180A publication Critical patent/JP2024525180A/ja
Publication of JP2024525180A5 publication Critical patent/JP2024525180A5/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023577938A 2021-06-17 2022-06-17 ペアワイズシーケンシングのための組成物及び方法 Pending JP2024525180A (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US202163212059P 2021-06-17 2021-06-17
US63/212,059 2021-06-17
US17/377,285 2021-07-15
US17/377,285 US11236388B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/377,284 2021-07-15
US17/377,279 2021-07-15
US17/377,279 US11535892B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/377,283 2021-07-15
US17/377,284 US11220707B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/377,283 US11427855B1 (en) 2021-06-17 2021-07-15 Compositions and methods for pairwise sequencing
US17/521,239 2021-11-08
US17/521,239 US11891651B2 (en) 2021-06-17 2021-11-08 Compositions and methods for pairwise sequencing
US202117554396A 2021-12-17 2021-12-17
US17/554,396 2021-12-17
PCT/US2022/034038 WO2022266470A1 (en) 2021-06-17 2022-06-17 Compositions and methods for pairwise sequencing

Publications (2)

Publication Number Publication Date
JP2024525180A true JP2024525180A (ja) 2024-07-10
JP2024525180A5 JP2024525180A5 (https=) 2025-06-25

Family

ID=89324679

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023577938A Pending JP2024525180A (ja) 2021-06-17 2022-06-17 ペアワイズシーケンシングのための組成物及び方法

Country Status (7)

Country Link
EP (1) EP4355913A1 (https=)
JP (1) JP2024525180A (https=)
KR (1) KR20240047357A (https=)
AU (1) AU2022294092A1 (https=)
CA (1) CA3224352A1 (https=)
GB (1) GB2623234B (https=)
IL (1) IL309338A (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025163526A1 (en) * 2024-01-30 2025-08-07 Element Biosciences, Inc. Method for improving nucleic acid sequencing quality by eliminating nucleic acids with deaminated bases from library and method for sequencing in which complexes of primers, polymerases and labelled probes are bound to concatemers

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013019361A1 (en) * 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
US10364464B2 (en) * 2011-08-08 2019-07-30 The Broad Institute, Inc. Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
US11085073B2 (en) * 2015-04-24 2021-08-10 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
JP7587425B2 (ja) * 2018-12-18 2024-11-20 イルミナ ケンブリッジ リミテッド 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物
KR20240094019A (ko) * 2019-09-23 2024-06-24 엘리먼트 바이오사이언스, 인크. 세포적으로 처리가능한 핵산 서열분석 방법
WO2022015600A2 (en) * 2020-07-13 2022-01-20 Singular Genomics Systems, Inc. Methods of sequencing complementary polynucleotides
KR20230091116A (ko) * 2020-10-21 2023-06-22 일루미나, 인코포레이티드 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법
CA3196800A1 (en) * 2020-10-30 2022-05-05 Element Biosciences, Inc. Reagents for massively parallel nucleic acid sequencing

Also Published As

Publication number Publication date
IL309338A (en) 2024-02-01
AU2022294092A1 (en) 2024-01-25
KR20240047357A (ko) 2024-04-12
GB2623234B (en) 2026-03-25
GB202400380D0 (en) 2024-02-28
EP4355913A1 (en) 2024-04-24
GB2623234A (en) 2024-04-10
CA3224352A1 (en) 2022-12-22

Similar Documents

Publication Publication Date Title
US20250236903A1 (en) Compositions and methods for pairwise sequencing
US11859241B2 (en) Compositions and methods for pairwise sequencing
US12421545B2 (en) Compositions and methods for preparing nucleic acid nanostructures using compaction oligonucleotides
WO2022266470A1 (en) Compositions and methods for pairwise sequencing
US20230193354A1 (en) Compositions and methods for pairwise sequencing
US11781185B2 (en) Methods and reagents for nucleic acid analysis
US12359193B2 (en) Single-stranded splint strands and methods of use
JP2025507898A (ja) 二本鎖スプリントアダプター及び使用方法
JP2025525422A (ja) 二本鎖スプリントアダプターを使用したpcrフリーライブラリ調製及び使用方法
JP2025530259A (ja) ユニバーサル長スプリント鎖を有する二本鎖スプリントアダプター及び使用方法
AU2024298639A1 (en) On-support circularization and amplification for generating immobilized nucleic acid concatemer molecules
KR20250141743A (ko) 효소 기반 시약을 사용한 주형 분자의 다중 영역 시퀀싱을 위한 조성물 및 방법
JP2025528821A (ja) 核酸の空間的に分解された表面キャプチャ
KR20250022076A (ko) 핵산 라이브러리로부터 탈아미노화된 뉴클레오타이드를 제거함으로써 염기 호출 오류를 감소시키는 조성물 및 방법
JP2024525180A (ja) ペアワイズシーケンシングのための組成物及び方法
CN118696133A (zh) 用于成对测序的组合物和方法
HK40117539A (zh) 用於成对测序的组合物和方法
HK40128283A (zh) 用於使用压实寡核苷酸制备核酸纳米结构的组合物和方法
KR20260058236A (ko) 고정화된 핵산 콘카테머 분자를 생성하기 위한 지지체상 원형화 및 증폭

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250617

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20250617