CA3209481A1 - Procedes et compositions de congelation et de decongelation de cellules de mammifere - Google Patents
Procedes et compositions de congelation et de decongelation de cellules de mammifere Download PDFInfo
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- CA3209481A1 CA3209481A1 CA3209481A CA3209481A CA3209481A1 CA 3209481 A1 CA3209481 A1 CA 3209481A1 CA 3209481 A CA3209481 A CA 3209481A CA 3209481 A CA3209481 A CA 3209481A CA 3209481 A1 CA3209481 A1 CA 3209481A1
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- 238000003026 viability measurement method Methods 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
La présente divulgation concerne, entre autres, un procédé de cryoconservation et de décongélation de cellules qui conduit à des cellules décongelées présentant une viabilité cellulaire et une fonctionnalité élevées après décongélation. Dans certains modes de réalisation, l'invention concerne un procédé à grande échelle de cryoconservation de cellules, le procédé consistant à : (a) mettre en contact les cellules avec un milieu de cryoconservation ; (b) refroidir les cellules à -80 °C à une vitesse régulée afin de réduire au minimum l'enthalpie de fusion ; et (c) stocker les cellules en phase vapeur d'azote liquide, ce qui permet de cryoconserver les cellules immunitaires.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202163147737P | 2021-02-09 | 2021-02-09 | |
US63/147,737 | 2021-02-09 | ||
USPCT/US22/15627 | 2022-02-08 | ||
PCT/US2022/015627 WO2022173737A1 (fr) | 2021-02-09 | 2022-02-08 | Procédés et compositions de congélation et de décongélation de cellules de mammifère |
PCT/US2022/015870 WO2022173867A1 (fr) | 2021-02-09 | 2022-02-09 | Procédés et compositions de congélation et de décongélation de cellules de mammifère |
Publications (1)
Publication Number | Publication Date |
---|---|
CA3209481A1 true CA3209481A1 (fr) | 2022-08-18 |
Family
ID=80785114
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3209481A Pending CA3209481A1 (fr) | 2021-02-09 | 2022-02-09 | Procedes et compositions de congelation et de decongelation de cellules de mammifere |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP4291028A1 (fr) |
JP (1) | JP2024506337A (fr) |
KR (1) | KR20230145095A (fr) |
AU (1) | AU2022218710A1 (fr) |
CA (1) | CA3209481A1 (fr) |
CO (1) | CO2023010467A2 (fr) |
IL (1) | IL305012A (fr) |
MX (1) | MX2023009266A (fr) |
WO (1) | WO2022173867A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117305104A (zh) * | 2023-09-04 | 2023-12-29 | 上海交通大学 | 一种多物理场科学实验系统 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5795711A (en) * | 1996-04-04 | 1998-08-18 | Circe Biomedical, Inc. | Cryopreserved hepatocytes and high viability and metabolic activity |
NZ605505A (en) * | 2010-07-13 | 2015-02-27 | Anthrogenesis Corp | Methods of generating natural killer cells |
-
2022
- 2022-02-09 JP JP2023548177A patent/JP2024506337A/ja active Pending
- 2022-02-09 IL IL305012A patent/IL305012A/en unknown
- 2022-02-09 EP EP22711117.6A patent/EP4291028A1/fr active Pending
- 2022-02-09 AU AU2022218710A patent/AU2022218710A1/en active Pending
- 2022-02-09 WO PCT/US2022/015870 patent/WO2022173867A1/fr active Application Filing
- 2022-02-09 KR KR1020237029571A patent/KR20230145095A/ko unknown
- 2022-02-09 CA CA3209481A patent/CA3209481A1/fr active Pending
- 2022-02-09 MX MX2023009266A patent/MX2023009266A/es unknown
-
2023
- 2023-08-10 CO CONC2023/0010467A patent/CO2023010467A2/es unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117305104A (zh) * | 2023-09-04 | 2023-12-29 | 上海交通大学 | 一种多物理场科学实验系统 |
Also Published As
Publication number | Publication date |
---|---|
CO2023010467A2 (es) | 2023-08-18 |
WO2022173867A1 (fr) | 2022-08-18 |
JP2024506337A (ja) | 2024-02-13 |
EP4291028A1 (fr) | 2023-12-20 |
AU2022218710A1 (en) | 2023-08-10 |
MX2023009266A (es) | 2023-08-17 |
KR20230145095A (ko) | 2023-10-17 |
IL305012A (en) | 2023-10-01 |
AU2022218710A2 (en) | 2023-11-23 |
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