CA3179549A1 - Analyse fondee sur des billes d'un echantillon - Google Patents

Analyse fondee sur des billes d'un echantillon

Info

Publication number
CA3179549A1
CA3179549A1 CA3179549A CA3179549A CA3179549A1 CA 3179549 A1 CA3179549 A1 CA 3179549A1 CA 3179549 A CA3179549 A CA 3179549A CA 3179549 A CA3179549 A CA 3179549A CA 3179549 A1 CA3179549 A1 CA 3179549A1
Authority
CA
Canada
Prior art keywords
beads
sample
chemical component
image sensor
bead
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3179549A
Other languages
English (en)
Inventor
Alan Marc Fine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alentic Microscience Inc
Original Assignee
Alentic Microscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/845,458 external-priority patent/US11255850B2/en
Application filed by Alentic Microscience Inc filed Critical Alentic Microscience Inc
Publication of CA3179549A1 publication Critical patent/CA3179549A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses

Abstract

Un procédé consiste à fixer au moins deux billes à chaque unité d'au moins une unité d'un composant chimique dans un échantillon pour former, pour chacune des unités d'un composant chimique, un complexe multi-billes comprenant au moins deux billes et l'unité du composant chimique ; à placer l'échantillon sur une surface d'un capteur d'images ; à recevoir, au niveau du capteur d'images, la lumière provenant d'une source lumineuse, la lumière reçue comprenant de la lumière réfléchie ou réfractée par les billes des complexes multi-billes ou transmise à travers ces dernières ; au niveau du capteur d'images, à capturer au moins une image de l'échantillon à partir de la lumière reçue ; et à identifier, dans au moins une des images de l'échantillon, des complexes multi-billes séparés, l'identification des complexes multi-billes séparés consistant à associer les au moins deux billes de chacun desdits complexes en fonction de leurs proximités mutuelles.
CA3179549A 2020-04-10 2021-04-08 Analyse fondee sur des billes d'un echantillon Pending CA3179549A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/845,458 US11255850B2 (en) 2019-03-28 2020-04-10 Bead-based analysis of a sample
US16/845,458 2020-04-10
PCT/CA2021/050466 WO2021203201A1 (fr) 2020-04-10 2021-04-08 Analyse fondée sur des billes d'un échantillon

Publications (1)

Publication Number Publication Date
CA3179549A1 true CA3179549A1 (fr) 2021-10-14

Family

ID=78022372

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3179549A Pending CA3179549A1 (fr) 2020-04-10 2021-04-08 Analyse fondee sur des billes d'un echantillon

Country Status (6)

Country Link
EP (1) EP4133259A4 (fr)
JP (1) JP2023520733A (fr)
KR (1) KR20220165759A (fr)
CN (1) CN115552225A (fr)
CA (1) CA3179549A1 (fr)
WO (1) WO2021203201A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11255850B2 (en) 2019-03-28 2022-02-22 Alentic Microscience Inc. Bead-based analysis of a sample
US11609233B2 (en) 2019-03-28 2023-03-21 Alentic Microscience Inc. Indicator-based analysis of a sample
US11719700B2 (en) 2019-03-28 2023-08-08 Alentic Microscience Inc. Upconversion for microscopy

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2473595A4 (fr) * 2009-08-31 2013-04-24 Mbio Diagnostics Inc Système intégré de préparation d'échantillons et de détection d'analytes
EP2536818B1 (fr) * 2010-02-18 2018-10-03 Bima Limited Dosage immunomultiplex à billes immobilisées
WO2013057634A1 (fr) * 2011-10-19 2013-04-25 Koninklijke Philips Electronics N.V. Détection d'agrégats de particules magnétiques
US10436778B2 (en) * 2013-03-15 2019-10-08 Plexbio Co., Ltd. Methods and systems for multiplex assays
WO2015139022A1 (fr) * 2014-03-14 2015-09-17 Northeastern University Système microfluidique et procédé de mesure en temps réel de liaison anticorps-antigène et de détection d'analyte
CN111183350A (zh) * 2017-08-17 2020-05-19 雅培医护站股份有限公司 用于对血细胞成像的单次使用测试设备
EP3717887A4 (fr) * 2017-11-28 2020-10-21 Alentic Microscience Inc. Classification de microbilles dans une imagerie en champ proche
US10684278B1 (en) * 2019-03-28 2020-06-16 Alentic Microscience Inc. Bead-based analysis of a sample

Also Published As

Publication number Publication date
CN115552225A (zh) 2022-12-30
WO2021203201A1 (fr) 2021-10-14
EP4133259A1 (fr) 2023-02-15
JP2023520733A (ja) 2023-05-18
KR20220165759A (ko) 2022-12-15
EP4133259A4 (fr) 2023-09-27

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