CA3118689A1 - Artificial expression constructs for selectively modulating gene expression in excitatory cortical neurons - Google Patents

Artificial expression constructs for selectively modulating gene expression in excitatory cortical neurons Download PDF

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Publication number
CA3118689A1
CA3118689A1 CA3118689A CA3118689A CA3118689A1 CA 3118689 A1 CA3118689 A1 CA 3118689A1 CA 3118689 A CA3118689 A CA 3118689A CA 3118689 A CA3118689 A CA 3118689A CA 3118689 A1 CA3118689 A1 CA 3118689A1
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ehgt
seq
cell
cells
expression
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CA3118689A
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English (en)
French (fr)
Inventor
Lucas T. GRAYBUCK
Bosiljka Tasic
Tanya DAIGLE
Jonathan Ting
Hongkui Zeng
Brian Edward KALMBACH
John K. Mich
Erik HESS
Edward Sebastian LEIN
Boaz P. LEVI
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Allen Institute
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Allen Institute
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3118689A 2018-11-05 2019-11-05 Artificial expression constructs for selectively modulating gene expression in excitatory cortical neurons Pending CA3118689A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201862755988P 2018-11-05 2018-11-05
US62/755,988 2018-11-05
US201962806600P 2019-02-15 2019-02-15
US201962806684P 2019-02-15 2019-02-15
US62/806,600 2019-02-15
US62/806,684 2019-02-15
US201962872021P 2019-07-09 2019-07-09
US62/872,021 2019-07-09
PCT/US2019/059927 WO2020097121A1 (en) 2018-11-05 2019-11-05 Artificial expression constructs for selectively modulating gene expression in excitatory cortical neurons

Publications (1)

Publication Number Publication Date
CA3118689A1 true CA3118689A1 (en) 2020-05-14

Family

ID=70612483

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3118689A Pending CA3118689A1 (en) 2018-11-05 2019-11-05 Artificial expression constructs for selectively modulating gene expression in excitatory cortical neurons

Country Status (6)

Country Link
US (2) US20210395780A1 (https=)
EP (1) EP3877529A4 (https=)
JP (3) JP7590962B2 (https=)
AU (2) AU2019375975B2 (https=)
CA (1) CA3118689A1 (https=)
WO (1) WO2020097121A1 (https=)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3923995A4 (en) * 2019-02-15 2023-05-17 Allen Institute ARTIFICIAL EXPRESSION RECOMBINANTS FOR THE SELECTIVE MODULATION OF GENE EXPRESSION IN SELECTED NEURON CELL POPULATIONS
JP7624728B2 (ja) 2019-02-25 2025-01-31 ユニバーシティ オブ マサチューセッツ Ｄｎａ結合ドメイントランスアクチベーター及びその使用
EP4162041A4 (en) * 2020-06-04 2024-10-16 Allen Institute ARTIFICIAL EXPRESSION CONSTRUCTS TO SELECTIVELY MODULATE GENE EXPRESSION IN INHIBITORY NEOCORTICAL NEURONS
WO2022020706A1 (en) * 2020-07-24 2022-01-27 University Of Massachusetts Dna-binding domain transactivators and uses thereof
WO2022036255A1 (en) * 2020-08-14 2022-02-17 Allen Institute Artificial expression constructs for modulating gene expression in striatal neurons
GB202013940D0 (en) 2020-09-04 2020-10-21 Synpromics Ltd Regulatory nucleic acid sequences
EP4244366A4 (en) * 2020-11-10 2024-12-25 Allen Institute Artificial expression constructs for modulating gene expression in chandelier cells
WO2022155358A1 (en) * 2021-01-13 2022-07-21 Duke University Compositions for and methods of improving fluid flux in the brain
US20240182923A1 (en) * 2021-03-30 2024-06-06 Allen Institute Artificial expression constructs for modulating gene expression in claustrum neurons
US20240254514A1 (en) * 2021-05-21 2024-08-01 Allen Institute Artificial expression constructs for modulating gene expression in neurons within the thalamus
CN113736734B (zh) * 2021-09-23 2022-10-25 中山大学中山眼科中心 一种用于灵长类动物神经元分离的试剂盒及方法
WO2023108021A1 (en) * 2021-12-07 2023-06-15 Allen Institute Artificial expression constructs for modulating gene expression in neocortical layer 4 or layer 5 intratelencephalic neurons
US20250163458A1 (en) * 2022-02-24 2025-05-22 Allen Institute Artificial expression constructs for modulating gene expression in dopaminergic neurons
CN114752624B (zh) * 2022-04-08 2025-04-15 中国海洋大学 一种侏儒蛤卵子的电穿孔方法
WO2024011224A2 (en) * 2022-07-08 2024-01-11 The Trustees Of Columbia University In The City Of New York Regulatory element for cell type specific expression of genes in spinal motor neurons
WO2024238448A2 (en) * 2023-05-12 2024-11-21 Temple University - Of The Commonwealth System Of Higher Education Chimeric capsid recombinant adeno-associated virus with bbb penetration and microglia targeting (aav-bm)
CN117346715B (zh) * 2023-12-04 2024-03-08 成都深瑞同华科技有限公司 一种配电网架空线路弧垂测定方法、系统及存储介质

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2028268A1 (en) * 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
WO2014080381A1 (en) * 2012-11-26 2014-05-30 Ecole Polytechnique Federale De Lausanne (Epfl) Colorectal cancer classification with differential prognosis and personalized therapeutic responses
CL2014003590A1 (es) * 2014-12-30 2015-07-10 Univ Chile Virus aav/xbp1s-ha, método de tratamiento genético y su uso en la optimizacion y mejoramiento de las capacidades cognitivas, de memoria y de aprendizaje.
EA201992358A1 (ru) * 2017-04-03 2020-03-24 Инкоудид Терапьютикс, Инк. Тканеселективная экспрессия трансгена

Also Published As

Publication number Publication date
JP2026021360A (ja) 2026-02-10
JP7590962B2 (ja) 2024-11-27
AU2025242185A1 (en) 2025-10-30
WO2020097121A1 (en) 2020-05-14
JP2025024092A (ja) 2025-02-19
US20250236886A1 (en) 2025-07-24
US20210395780A1 (en) 2021-12-23
AU2019375975B2 (en) 2025-07-10
EP3877529A4 (en) 2023-01-25
AU2019375975A1 (en) 2021-06-17
EP3877529A1 (en) 2021-09-15
JP7774695B2 (ja) 2025-11-21
JP2022513426A (ja) 2022-02-08

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