CA3107517A1 - Esterases and uses thereof - Google Patents

Esterases and uses thereof Download PDF

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Publication number
CA3107517A1
CA3107517A1 CA3107517A CA3107517A CA3107517A1 CA 3107517 A1 CA3107517 A1 CA 3107517A1 CA 3107517 A CA3107517 A CA 3107517A CA 3107517 A CA3107517 A CA 3107517A CA 3107517 A1 CA3107517 A1 CA 3107517A1
Authority
CA
Canada
Prior art keywords
esterase
seq
amino acid
polyester
substitution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3107517A
Other languages
English (en)
French (fr)
Inventor
Benoit David
Isabelle Andre
Maher BEN KHALED
Sophie Duquesne
Alain Marty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carbios SA
Original Assignee
Carbios SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbios SA filed Critical Carbios SA
Publication of CA3107517A1 publication Critical patent/CA3107517A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/04—Polyesters derived from hydroxy carboxylic acids, e.g. lactones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
CA3107517A 2018-07-27 2019-07-26 Esterases and uses thereof Pending CA3107517A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18306018.5 2018-07-27
EP18306018 2018-07-27
PCT/EP2019/070287 WO2020021116A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (1)

Publication Number Publication Date
CA3107517A1 true CA3107517A1 (en) 2020-01-30

Family

ID=63144937

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3107517A Pending CA3107517A1 (en) 2018-07-27 2019-07-26 Esterases and uses thereof

Country Status (11)

Country Link
US (2) US11535832B2 (https=)
EP (2) EP4424823B1 (https=)
JP (2) JP7519987B2 (https=)
KR (2) KR20260004534A (https=)
CN (2) CN120173910A (https=)
CA (1) CA3107517A1 (https=)
ES (1) ES3006610T3 (https=)
LT (1) LT3830254T (https=)
MX (1) MX2021001046A (https=)
PT (1) PT3830254T (https=)
WO (1) WO2020021116A1 (https=)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107709457B (zh) 2015-06-12 2021-05-25 卡比奥斯公司 可生物降解聚酯组合物及其用途
MX2018014090A (es) 2016-05-19 2019-04-01 Carbios Un proceso para degradar productos de plastico.
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
BR112019012258A2 (pt) 2016-12-16 2020-10-20 Carbios proteases de degradação de plástico aprimoradas
JP7217267B2 (ja) 2017-08-31 2023-02-02 キャルビオス 生物学的実体を含む液体組成物及びその使用
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
JP7601756B2 (ja) 2018-07-27 2024-12-17 キャルビオス 新規なエステラーゼ及びその使用
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
US11692181B2 (en) 2018-07-27 2023-07-04 Carbios Esterases and uses thereof
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
CA3129549A1 (en) 2019-03-28 2020-10-01 Carbios Multicomponent thermoplastic product
CA3145635A1 (en) 2019-07-11 2021-01-14 Carbios Esterases and uses thereof
JP7638952B2 (ja) * 2019-07-11 2025-03-04 キャルビオス 新規エステラーゼ及びその使用
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
WO2022090288A1 (en) * 2020-10-27 2022-05-05 Carbios Novel esterases and uses thereof
CN116615491A (zh) 2020-10-27 2023-08-18 卡比奥斯公司 新酯酶及其用途
CN114438140B (zh) * 2020-11-02 2024-05-17 华南理工大学 Pet的降解方法
AU2022277645A1 (en) 2021-05-20 2023-11-30 Carbios Process for degrading a plastic product comprising at least one polyester
CN117413007A (zh) 2021-05-20 2024-01-16 卡比奥斯公司 降解包含至少一种聚酯的塑料产品的方法
CN117597424A (zh) * 2021-06-30 2024-02-23 汉高股份有限及两合公司 具有改进的水分管理性能的组合物
CN113698742B (zh) * 2021-08-31 2022-11-22 江南大学 一种聚对苯二甲酸乙二醇酯的改性方法及其在酶解过程中的应用
WO2023088909A2 (en) * 2021-11-16 2023-05-25 Carbios Novel esterases and uses thereof
US20250019678A1 (en) * 2021-11-16 2025-01-16 Carbios Esterases and uses thereof
CN114891767B (zh) * 2022-05-19 2023-04-28 华南理工大学 角质酶-酯酶融合蛋白及其应用
WO2023242197A1 (en) 2022-06-13 2023-12-21 Carbios Process for purifying mono-ethylene glycol
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
US20240228727A1 (en) 2023-01-09 2024-07-11 Protein Evolution Inc. Systems and methods for pretreatment and/or enzymatic degradation of crystallizable polymers, including copolymers
US20240352064A1 (en) 2023-03-21 2024-10-24 Protein Evolution Inc. Methods and compositions for the separation of particles from a fluid
TW202511480A (zh) 2023-05-15 2025-03-16 法商卡爾畢歐斯公司 新穎之酯酶及其用途
CN121175416A (zh) 2023-05-15 2025-12-19 卡比奥斯公司 酯酶变体及其在聚酯降解中的用途
IT202300014223A1 (it) * 2023-07-07 2025-01-07 Milano Politecnico Enzima ingegnerizzato termostabile
CN120905186B (zh) * 2025-09-28 2026-03-17 源天生物科技(天津)有限公司 BhrPETase突变体及其应用

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
EP2751263A1 (en) * 2011-08-31 2014-07-09 Danisco US Inc. Compositions and methods comprising a lipolytic enzyme variant
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
BR112014014410A2 (pt) * 2011-12-22 2019-09-24 Danisco Us Inc composições e métodos que compreendem uma variante de enzima lipolítica
EP2922906B1 (en) 2012-11-20 2018-10-31 Carbios Method for recycling plastic products
EP3065890A2 (en) 2013-11-05 2016-09-14 Carbios A method for degrading a plastic
DE102014204374A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa PET-Esterasen und deren Verwendung
CA2947478C (en) 2014-05-16 2022-09-13 Carbios Process of recycling mixed pet plastic articles
TN2017000085A1 (en) 2014-10-21 2018-07-04 Carbios Polypeptide having a polyester degrading activity and uses thereof
US10626242B2 (en) 2014-12-19 2020-04-21 Carbios Plastic compound and preparation process
EP3268469A1 (en) 2015-03-13 2018-01-17 Carbios New polypeptide having a polyester degrading activity and uses thereof
CN107709457B (zh) 2015-06-12 2021-05-25 卡比奥斯公司 可生物降解聚酯组合物及其用途
US10717996B2 (en) 2015-12-21 2020-07-21 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
MX2018014090A (es) 2016-05-19 2019-04-01 Carbios Un proceso para degradar productos de plastico.
WO2018011284A1 (en) * 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
CA3030469C (en) * 2016-07-12 2024-04-30 Carbios Esterases and uses thereof
BR112019012258A2 (pt) 2016-12-16 2020-10-20 Carbios proteases de degradação de plástico aprimoradas
DE102017214870A1 (de) * 2017-08-24 2019-03-14 Henkel Ag & Co. Kgaa Verbesserte Pflegeeigenschaften von Polyester Textilien II
WO2019043134A1 (en) 2017-08-31 2019-03-07 Carbiolice BIODEGRADABLE POLYESTER ARTICLE COMPRISING ENZYMES
JP7217267B2 (ja) 2017-08-31 2023-02-02 キャルビオス 生物学的実体を含む液体組成物及びその使用
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
DE102018210609A1 (de) 2018-06-28 2020-01-02 Henkel Ag & Co. Kgaa Polyesterase II
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
JP7601756B2 (ja) 2018-07-27 2024-12-17 キャルビオス 新規なエステラーゼ及びその使用
US11692181B2 (en) 2018-07-27 2023-07-04 Carbios Esterases and uses thereof
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
FR3088069B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de production d'acide terephtalique a l'echelle industrielle
CA3129549A1 (en) 2019-03-28 2020-10-01 Carbios Multicomponent thermoplastic product
JP7638952B2 (ja) 2019-07-11 2025-03-04 キャルビオス 新規エステラーゼ及びその使用
CA3145635A1 (en) 2019-07-11 2021-01-14 Carbios Esterases and uses thereof
EP4061934A1 (en) 2019-11-18 2022-09-28 Carbios Novel proteases and uses thereof
WO2021123299A1 (en) 2019-12-19 2021-06-24 Carbios Process for degrading plastic products
EP3838976B1 (en) 2019-12-19 2025-02-12 Carbios Process for degrading plastic products
WO2021123328A1 (en) 2019-12-19 2021-06-24 Carbios Foamed plastic compositions

Also Published As

Publication number Publication date
US11926851B2 (en) 2024-03-12
EP4424823C0 (en) 2025-12-03
KR20260004534A (ko) 2026-01-08
US20210163906A1 (en) 2021-06-03
EP4424823B1 (en) 2025-12-03
CN120173910A (zh) 2025-06-20
JP2021531030A (ja) 2021-11-18
CN112654701A (zh) 2021-04-13
US20230212537A1 (en) 2023-07-06
EP3830254A1 (en) 2021-06-09
PT3830254T (pt) 2025-01-24
US11535832B2 (en) 2022-12-27
LT3830254T (lt) 2025-03-10
KR102899059B1 (ko) 2025-12-11
WO2020021116A1 (en) 2020-01-30
CN112654701B (zh) 2025-04-08
KR20210042336A (ko) 2021-04-19
EP4424823A2 (en) 2024-09-04
EP4424823A3 (en) 2024-12-25
ES3006610T3 (en) 2025-03-18
MX2021001046A (es) 2021-04-12
JP7519987B2 (ja) 2024-07-22
EP3830254B1 (en) 2024-12-11
JP2024073628A (ja) 2024-05-29

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