CA3107517A1 - Esterases and uses thereof - Google Patents

Esterases and uses thereof Download PDF

Info

Publication number
CA3107517A1
CA3107517A1 CA3107517A CA3107517A CA3107517A1 CA 3107517 A1 CA3107517 A1 CA 3107517A1 CA 3107517 A CA3107517 A CA 3107517A CA 3107517 A CA3107517 A CA 3107517A CA 3107517 A1 CA3107517 A1 CA 3107517A1
Authority
CA
Canada
Prior art keywords
esterase
seq
amino acid
polyester
substitution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3107517A
Other languages
English (en)
French (fr)
Inventor
Benoit David
Isabelle Andre
Maher BEN KHALED
Sophie Duquesne
Alain Marty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carbios SA
Original Assignee
Carbios SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbios SA filed Critical Carbios SA
Publication of CA3107517A1 publication Critical patent/CA3107517A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/04—Polyesters derived from hydroxy carboxylic acids, e.g. lactones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
CA3107517A 2018-07-27 2019-07-26 Esterases and uses thereof Pending CA3107517A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18306018 2018-07-27
EP18306018.5 2018-07-27
PCT/EP2019/070287 WO2020021116A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (1)

Publication Number Publication Date
CA3107517A1 true CA3107517A1 (en) 2020-01-30

Family

ID=63144937

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3107517A Pending CA3107517A1 (en) 2018-07-27 2019-07-26 Esterases and uses thereof

Country Status (11)

Country Link
US (2) US11535832B2 (enExample)
EP (2) EP3830254B1 (enExample)
JP (2) JP7519987B2 (enExample)
KR (2) KR102899059B1 (enExample)
CN (2) CN112654701B (enExample)
CA (1) CA3107517A1 (enExample)
ES (1) ES3006610T3 (enExample)
LT (1) LT3830254T (enExample)
MX (1) MX2021001046A (enExample)
PT (1) PT3830254T (enExample)
WO (1) WO2020021116A1 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
EP3485007B1 (en) 2016-07-12 2025-10-29 Carbios Novel esterases and uses thereof
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
CN112673104B (zh) 2018-07-27 2025-02-25 卡比奥斯公司 新酯酶及其用途
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
CA3107560A1 (en) 2018-07-27 2020-01-30 Carbios Esterases and uses thereof
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
AU2020249603A1 (en) 2019-03-28 2021-09-23 Carbios Multicomponent thermoplastic product
EP3997220B1 (en) 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
EP3997219B1 (en) 2019-07-11 2025-08-06 Carbios Novel esterases and uses thereof
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
KR20230093468A (ko) 2020-10-27 2023-06-27 까르비오 신규한 에스테라아제 및 그 용도
JP2023546506A (ja) * 2020-10-27 2023-11-02 キャルビオス 新規エステラーゼ及びその使用
CN114438140B (zh) * 2020-11-02 2024-05-17 华南理工大学 Pet的降解方法
CA3218310A1 (en) 2021-05-20 2022-11-24 Carbios Process for degrading a plastic product comprising at least one polyester
MX2023013755A (es) 2021-05-20 2023-12-04 Carbios Proceso para degradar un producto de plastico que comprende al menos un poliester.
EP4363543A1 (en) * 2021-06-30 2024-05-08 Henkel AG & Co. KGaA Composition with improved moisture management performance
CN113698742B (zh) * 2021-08-31 2022-11-22 江南大学 一种聚对苯二甲酸乙二醇酯的改性方法及其在酶解过程中的应用
MX2024005891A (es) * 2021-11-16 2024-05-30 Carbios Esterasas y usos de las mismas.
EP4433582A2 (en) * 2021-11-16 2024-09-25 Carbios Esterases and uses thereof
CN114891767B (zh) * 2022-05-19 2023-04-28 华南理工大学 角质酶-酯酶融合蛋白及其应用
WO2023242197A1 (en) 2022-06-13 2023-12-21 Carbios Process for purifying mono-ethylene glycol
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
WO2024151608A2 (en) 2023-01-09 2024-07-18 Protein Evolution Inc. Systems, methods, and compositions involving pretreatment and/or enzymatic degradation of crystallizable polymers, including copolymers
TW202506250A (zh) 2023-03-21 2025-02-16 美商蛋白質演化股份有限公司 從流體中分離顆粒的方法和組合物
KR20260011740A (ko) 2023-05-15 2026-01-23 까르비오 신규 에스테라제 및 이의 용도
WO2024236009A2 (en) 2023-05-15 2024-11-21 Carbios Novel esterases and uses thereof
IT202300014223A1 (it) * 2023-07-07 2025-01-07 Milano Politecnico Enzima ingegnerizzato termostabile
CN120905186B (zh) * 2025-09-28 2026-03-17 源天生物科技(天津)有限公司 BhrPETase突变体及其应用

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5850342B2 (ja) 2011-01-19 2016-02-03 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
EP2751263A1 (en) * 2011-08-31 2014-07-09 Danisco US Inc. Compositions and methods comprising a lipolytic enzyme variant
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
CN104024407A (zh) * 2011-12-22 2014-09-03 丹尼斯科美国公司 包含脂解酶变体的组合物和方法
ES2707304T3 (es) 2012-11-20 2019-04-03 Carbios Método para reciclar productos de plástico
EP3065890A2 (en) 2013-11-05 2016-09-14 Carbios A method for degrading a plastic
DE102014204374A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa PET-Esterasen und deren Verwendung
CA2947478C (en) 2014-05-16 2022-09-13 Carbios Process of recycling mixed pet plastic articles
TN2017000085A1 (en) 2014-10-21 2018-07-04 Carbios Polypeptide having a polyester degrading activity and uses thereof
WO2016097325A1 (en) 2014-12-19 2016-06-23 Carbios Plastic compound and preparation process
CN107532153A (zh) 2015-03-13 2018-01-02 卡比奥斯公司 具有聚酯降解活性的新多肽及其用途
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
US10717996B2 (en) 2015-12-21 2020-07-21 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
EP3485007B1 (en) 2016-07-12 2025-10-29 Carbios Novel esterases and uses thereof
MX2019000493A (es) * 2016-07-12 2019-04-01 Carbios Nuevas esterasas y usos de las mismas.
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
DE102017214870A1 (de) * 2017-08-24 2019-03-14 Henkel Ag & Co. Kgaa Verbesserte Pflegeeigenschaften von Polyester Textilien II
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
BR112020004040B1 (pt) 2017-08-31 2023-10-24 Carbiolice Artigo de poliéster biodegradável compreendendo enzimas
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
DE102018210609A1 (de) 2018-06-28 2020-01-02 Henkel Ag & Co. Kgaa Polyesterase II
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
CA3107560A1 (en) 2018-07-27 2020-01-30 Carbios Esterases and uses thereof
CN112673104B (zh) 2018-07-27 2025-02-25 卡比奥斯公司 新酯酶及其用途
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
FR3088069B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de production d'acide terephtalique a l'echelle industrielle
AU2020249603A1 (en) 2019-03-28 2021-09-23 Carbios Multicomponent thermoplastic product
EP3997220B1 (en) 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
EP3997219B1 (en) 2019-07-11 2025-08-06 Carbios Novel esterases and uses thereof
WO2021099337A1 (en) 2019-11-18 2021-05-27 Carbios Novel proteases and uses thereof
EP4077502A1 (en) 2019-12-19 2022-10-26 Carbios Foamed plastic compositions
KR20220119059A (ko) 2019-12-19 2022-08-26 까르비오 플라스틱 제품을 분해하기 위한 방법
EP3838976B1 (en) 2019-12-19 2025-02-12 Carbios Process for degrading plastic products

Also Published As

Publication number Publication date
EP4424823C0 (en) 2025-12-03
JP2021531030A (ja) 2021-11-18
EP3830254A1 (en) 2021-06-09
MX2021001046A (es) 2021-04-12
PT3830254T (pt) 2025-01-24
EP4424823B1 (en) 2025-12-03
KR20260004534A (ko) 2026-01-08
ES3006610T3 (en) 2025-03-18
US11535832B2 (en) 2022-12-27
US11926851B2 (en) 2024-03-12
JP2024073628A (ja) 2024-05-29
KR20210042336A (ko) 2021-04-19
EP3830254B1 (en) 2024-12-11
CN120173910A (zh) 2025-06-20
KR102899059B1 (ko) 2025-12-11
CN112654701A (zh) 2021-04-13
CN112654701B (zh) 2025-04-08
US20230212537A1 (en) 2023-07-06
EP4424823A3 (en) 2024-12-25
WO2020021116A1 (en) 2020-01-30
US20210163906A1 (en) 2021-06-03
EP4424823A2 (en) 2024-09-04
JP7519987B2 (ja) 2024-07-22
LT3830254T (lt) 2025-03-10

Similar Documents

Publication Publication Date Title
US11926851B2 (en) Esterases and uses thereof
US11692181B2 (en) Esterases and uses thereof
US11851689B2 (en) Esterases and uses thereof
US12344714B2 (en) Esterases and uses thereof
WO2021005199A1 (en) Novel esterases and uses thereof
AU2021367905A1 (en) Novel esterases and uses thereof
AU2021370362A1 (en) Novel esterases and uses thereof
WO2022090290A1 (en) Novel esterases and uses thereof
EP4237548A1 (en) Novel esterases and uses thereof
US20250027063A1 (en) Novel esterases and uses thereof
US20250019678A1 (en) Esterases and uses thereof

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220919

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD

Year of fee payment: 5

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20250620

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD

Year of fee payment: 6

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250701

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250701

B12 Application deemed to be withdrawn, abandoned or lapsed

Free format text: ST27 STATUS EVENT CODE: N-2-6-B10-B12-B303 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO AN EXAMINER'S REQUISITION

Effective date: 20251020