CA3084812A1 - Dpo4 polymerase variants with improved accuracy - Google Patents

Dpo4 polymerase variants with improved accuracy Download PDF

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Publication number
CA3084812A1
CA3084812A1 CA3084812A CA3084812A CA3084812A1 CA 3084812 A1 CA3084812 A1 CA 3084812A1 CA 3084812 A CA3084812 A CA 3084812A CA 3084812 A CA3084812 A CA 3084812A CA 3084812 A1 CA3084812 A1 CA 3084812A1
Authority
CA
Canada
Prior art keywords
polymerase
amino acid
polymerases
mutation
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3084812A
Other languages
English (en)
French (fr)
Inventor
Mark Kokoris
Marc PRINDLE
Gregory Scott THIESSEN
Alexander Isaac LEHMANN
Drew GOODMAN
Aaron Jacobs
John Chase
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Stratos Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratos Genomics Inc filed Critical Stratos Genomics Inc
Publication of CA3084812A1 publication Critical patent/CA3084812A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3084812A 2017-12-11 2018-12-10 Dpo4 polymerase variants with improved accuracy Pending CA3084812A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762597109P 2017-12-11 2017-12-11
US62/597,109 2017-12-11
US201862656696P 2018-04-12 2018-04-12
US62/656,696 2018-04-12
PCT/US2018/064794 WO2019118372A1 (en) 2017-12-11 2018-12-10 Dpo4 polymerase variants with improved accuracy

Publications (1)

Publication Number Publication Date
CA3084812A1 true CA3084812A1 (en) 2019-06-20

Family

ID=66819492

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3084812A Pending CA3084812A1 (en) 2017-12-11 2018-12-10 Dpo4 polymerase variants with improved accuracy

Country Status (7)

Country Link
US (1) US11530392B2 (https=)
EP (1) EP3724323A4 (https=)
JP (1) JP7288457B2 (https=)
CN (1) CN111655846B (https=)
AU (1) AU2018383610B2 (https=)
CA (1) CA3084812A1 (https=)
WO (1) WO2019118372A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10745685B2 (en) 2015-11-16 2020-08-18 Stratos Genomics, Inc. DP04 polymerase variants
WO2018204707A1 (en) 2017-05-04 2018-11-08 Stratos Genomics Inc. Dp04 polymerase variants
CA3084812A1 (en) 2017-12-11 2019-06-20 Stratos Genomics, Inc. Dpo4 polymerase variants with improved accuracy
EP3972976A4 (en) 2019-05-23 2023-07-19 Stratos Genomics Inc. TRANSLOCATION CONTROL ELEMENTS, REPORTER CODES AND OTHER TRANSLOCATION CONTROL MEANS FOR USE IN NANOPOORE SEQUENCING
JP7354307B2 (ja) * 2019-06-26 2023-10-02 ストラトス ゲノミクス インコーポレイテッド 芳香族化合物による核酸重合の増強
WO2024083982A1 (en) 2022-10-21 2024-04-25 F. Hoffmann-La Roche Ag Detection of modified nucleobases in nucleic acid samples
WO2025082960A1 (en) 2023-10-18 2025-04-24 F. Hoffmann-La Roche Ag Dpo4 polymerase variants with improved properties
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025132780A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025149478A1 (en) 2024-01-12 2025-07-17 F. Hoffmann-La Roche Ag Compositions of modified nucleoside triphosphates
WO2025212586A1 (en) 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads
WO2026033006A1 (en) 2024-08-07 2026-02-12 Roche Sequencing Solutions, Inc. Nickase-mediated linear amplification of library constructs for duplex sequencing
WO2026050121A1 (en) 2024-08-30 2026-03-05 Roche Sequencing Solutions, Inc. Compositions for replicating a nucleic acid template

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9106702A (pt) 1990-07-27 1993-06-08 Isis Pharmaceuticals Inc Analogo de oligonucleotideos e processos para modular a producao de uma proteina por um organismo e para tratar um organismo
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
AU3222793A (en) 1991-11-26 1993-06-28 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
ES2105698T3 (es) 1993-03-30 1997-10-16 Sanofi Sa Oligonucleotidos modificados con 7-deazapurina.
AU6632094A (en) 1993-04-19 1994-11-08 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
EP2145956B1 (en) 2003-11-03 2015-06-17 Medical Research Council Polymerase
US7745188B2 (en) 2004-05-20 2010-06-29 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Thermostable Y-family polymerases and chimeras
US8921086B2 (en) 2005-12-22 2014-12-30 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
ES2959127T3 (es) 2007-06-19 2024-02-20 Hoffmann La Roche Secuenciación de ácidos nucleicos de alto rendimiento por expansión
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
KR102168915B1 (ko) 2012-05-16 2020-10-23 아프타리온 바이오테크 아게 Ｌ-핵산의 효소적 합성
WO2015074756A1 (en) * 2013-11-20 2015-05-28 Noxxon Pharma Ag Synthesis of l-nucleic acids by means of an enzyme
US10301345B2 (en) 2014-11-20 2019-05-28 Stratos Genomics, Inc. Phosphoroamidate esters, and use and synthesis thereof
US10526588B2 (en) 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
US10745685B2 (en) 2015-11-16 2020-08-18 Stratos Genomics, Inc. DP04 polymerase variants
CN106318924B (zh) 2016-10-26 2018-10-16 江南大学 一种催化dna合成延伸能力提高的dna聚合酶
WO2018204707A1 (en) * 2017-05-04 2018-11-08 Stratos Genomics Inc. Dp04 polymerase variants
CA3084812A1 (en) 2017-12-11 2019-06-20 Stratos Genomics, Inc. Dpo4 polymerase variants with improved accuracy

Also Published As

Publication number Publication date
JP2021505201A (ja) 2021-02-18
AU2018383610B2 (en) 2024-09-12
CN111655846A (zh) 2020-09-11
CN111655846B (zh) 2024-05-03
EP3724323A4 (en) 2021-08-18
US20210163903A1 (en) 2021-06-03
JP7288457B2 (ja) 2023-06-07
WO2019118372A1 (en) 2019-06-20
EP3724323A1 (en) 2020-10-21
AU2018383610A1 (en) 2020-07-23
US11530392B2 (en) 2022-12-20

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