CA3037217A1 - Analysis system and method for testing a sample - Google Patents
Analysis system and method for testing a sample Download PDFInfo
- Publication number
- CA3037217A1 CA3037217A1 CA3037217A CA3037217A CA3037217A1 CA 3037217 A1 CA3037217 A1 CA 3037217A1 CA 3037217 A CA3037217 A CA 3037217A CA 3037217 A CA3037217 A CA 3037217A CA 3037217 A1 CA3037217 A1 CA 3037217A1
- Authority
- CA
- Canada
- Prior art keywords
- sensor
- capture
- nucleic
- target
- assay
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Electrochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Fluid Mechanics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16020373.3 | 2016-10-07 | ||
| EP16020373 | 2016-10-07 | ||
| PCT/EP2017/025281 WO2018065104A1 (en) | 2016-10-07 | 2017-10-05 | Analysis system and method for testing a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3037217A1 true CA3037217A1 (en) | 2018-04-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3037217A Abandoned CA3037217A1 (en) | 2016-10-07 | 2017-10-05 | Analysis system and method for testing a sample |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10597703B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3523030B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2019536441A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20190066037A (cg-RX-API-DMAC7.html) |
| CN (1) | CN110114145B (cg-RX-API-DMAC7.html) |
| BR (1) | BR112019007081A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA3037217A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2018065104A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (8)
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| US11268134B2 (en) * | 2017-09-29 | 2022-03-08 | Boehringer Ingelheim Vetmedica Gmbh | Sensor apparatus and method for testing a sample |
| US20210189506A1 (en) | 2017-12-20 | 2021-06-24 | Boehringer Ingelheim Vetmedica Gmbh | Detection of modified live swine influenza virus vaccines |
| KR20210078510A (ko) | 2018-10-11 | 2021-06-28 | 버클리 라잇츠, 인크. | 최적화된 단백질 생성의 확인을 위한 시스템 및 방법, 및 이를 위한 키트 |
| US12411102B2 (en) | 2019-01-11 | 2025-09-09 | Fredsense Technologies Corp. | Systems and methods for voltammetric detection |
| US20240036039A1 (en) * | 2019-10-04 | 2024-02-01 | University Of Cincinnati | Shelf-stable, ready-to-use, electrochemical aptamer sensors |
| CN116368238A (zh) * | 2020-09-24 | 2023-06-30 | 伊诺泰科精密医疗股份有限公司 | 用于检测病原体的系统、设备和方法 |
| WO2022128917A1 (en) | 2020-12-14 | 2022-06-23 | Boehringer Ingelheim Vetmedica Gmbh | Analysis system and method for testing a sample |
| WO2023210026A1 (en) * | 2022-04-26 | 2023-11-02 | The University Of Tokyo | Sensor |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5096669A (en) | 1988-09-15 | 1992-03-17 | I-Stat Corporation | Disposable sensing device for real time fluid analysis |
| WO2000034521A1 (en) * | 1998-12-08 | 2000-06-15 | Boston Probes, Inc. | Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices |
| WO2000043552A2 (en) * | 1999-01-25 | 2000-07-27 | Ut-Battelle, Llc | Multifunctional and multispectral biosensor devices and methods of use |
| US6942771B1 (en) * | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
| AT410444B (de) | 2001-03-02 | 2003-04-25 | Oesterr Forsch Seibersdorf | Verfahren zur detektion von nukleinsäuremolekülen |
| DE10111457B4 (de) | 2001-03-09 | 2006-12-14 | Siemens Ag | Diagnoseeinrichtung |
| EP1439910A2 (en) * | 2001-07-26 | 2004-07-28 | Motorola, Inc. | System and methods for mixing within a microfluidic device |
| EP1636599B1 (de) | 2003-05-13 | 2007-05-02 | Siemens Aktiengesellschaft | Schaltkreisanordnung zur potentialkonstanthaltung an einem biosensor und zur digitalisierung des messstroms |
| CN1890566B (zh) * | 2003-12-26 | 2011-07-20 | 松下电器产业株式会社 | 生物样品鉴别装置、生物样品鉴别方法以及生物样品鉴别用平板 |
| PL1883474T3 (pl) | 2005-05-25 | 2021-10-18 | Boehringer Ingelheim Vetmedica Gmbh | System do zintegrowanej i zautomatyzowanej analizy dna lub białka oraz sposób działania takiego systemu |
| DE102005059535B4 (de) | 2005-12-13 | 2010-01-14 | Siemens Ag | Vorrichtung und Verfahren zur Durchführung eines Nukleinsäure-Tests, sowie Verfahren zur Herstellung einer solchen Vorrichtung |
| GB2436616A (en) | 2006-03-29 | 2007-10-03 | Inverness Medical Switzerland | Assay device and method |
| WO2008147382A1 (en) | 2006-09-27 | 2008-12-04 | Micronics, Inc. | Integrated microfluidic assay devices and methods |
| EP1947197A1 (en) | 2007-08-24 | 2008-07-23 | Siemens Aktiengesellschaft, A German Corporation | Method for the amplification of at least one nucleic acid |
| US8741815B2 (en) | 2008-02-19 | 2014-06-03 | Intelligent Bio Systems, Inc. | Methods and devices for amplification of nucleic acid |
| WO2010105207A1 (en) * | 2009-03-13 | 2010-09-16 | The Regents Of The University Of California | Prebiotic oligosaccharides |
| US9651568B2 (en) * | 2009-11-23 | 2017-05-16 | Cyvek, Inc. | Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays |
| DE102011015184B4 (de) | 2010-06-02 | 2013-11-21 | Thinxxs Microtechnology Ag | Vorrichtung für den Transport kleiner Volumina eines Fluids, insbesondere Mikropumpe oder Mikroventil |
| EP3644058A1 (en) * | 2010-10-14 | 2020-04-29 | Meso Scale Technologies, LLC | Reagent storage in an assay device |
| EP2705133A4 (en) * | 2011-05-02 | 2014-09-24 | Ibis Biosciences Inc | DEVICE AND SYSTEM FOR ASSAYS WITH SEVERAL ANALYSTS |
| US20130067525A1 (en) | 2011-09-09 | 2013-03-14 | Pct International, Inc. | Service provisioning device with integrated cable modem |
| WO2013067525A2 (en) * | 2011-11-04 | 2013-05-10 | President And Fellows Of Harvard College | Self-regulating chemo-mechano-chemical systems |
| US20130203057A1 (en) | 2012-01-20 | 2013-08-08 | Biohelix Corporation | Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions |
| WO2013161964A1 (ja) * | 2012-04-27 | 2013-10-31 | キリンホールディングス株式会社 | リガンドを高感度に検出する核酸分子並びに該核酸分子のスクリーニング方法および該核酸分子の感度の最適化方法 |
| US9217179B2 (en) * | 2012-05-24 | 2015-12-22 | The Governing Council Of The University Of Toronto | Systems and methods for multiplexed electrochemical detection |
| US9624532B2 (en) * | 2013-02-05 | 2017-04-18 | Neil Gordon | Ultra-sensitive detection of extremely low level biological analytes using electrochemical signal amplification and biosensor |
| WO2014164933A1 (en) * | 2013-03-11 | 2014-10-09 | Ruubix, Inc. | Systems and methods for detection and quantification of analytes |
| US20160223536A1 (en) * | 2013-10-10 | 2016-08-04 | Song Diagnostic Research Llc | Improved Lateral Flow Assays |
| GB201320071D0 (en) | 2013-11-13 | 2013-12-25 | Univ Leuven Kath | Monitoring multiplex DNA amplification |
-
2017
- 2017-10-05 KR KR1020197012881A patent/KR20190066037A/ko not_active Ceased
- 2017-10-05 CN CN201780061349.8A patent/CN110114145B/zh not_active Expired - Fee Related
- 2017-10-05 JP JP2019518541A patent/JP2019536441A/ja not_active Ceased
- 2017-10-05 CA CA3037217A patent/CA3037217A1/en not_active Abandoned
- 2017-10-05 WO PCT/EP2017/025281 patent/WO2018065104A1/en not_active Ceased
- 2017-10-05 EP EP17784848.8A patent/EP3523030B1/en active Active
- 2017-10-05 US US15/725,332 patent/US10597703B2/en not_active Expired - Fee Related
- 2017-10-05 BR BR112019007081A patent/BR112019007081A2/pt not_active Application Discontinuation
-
2020
- 2020-02-07 US US16/784,446 patent/US10988799B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP3523030B1 (en) | 2021-05-05 |
| US20200172965A1 (en) | 2020-06-04 |
| JP2019536441A (ja) | 2019-12-19 |
| US20180100188A1 (en) | 2018-04-12 |
| US10988799B2 (en) | 2021-04-27 |
| US10597703B2 (en) | 2020-03-24 |
| BR112019007081A2 (pt) | 2019-07-09 |
| WO2018065104A1 (en) | 2018-04-12 |
| CN110114145B (zh) | 2022-08-09 |
| EP3523030A1 (en) | 2019-08-14 |
| CN110114145A (zh) | 2019-08-09 |
| KR20190066037A (ko) | 2019-06-12 |
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Legal Events
| Date | Code | Title | Description |
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| FZDE | Discontinued |
Effective date: 20240116 |