CA3013052A1 - Dispositifs de perfusion acoustique - Google Patents
Dispositifs de perfusion acoustique Download PDFInfo
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- CA3013052A1 CA3013052A1 CA3013052A CA3013052A CA3013052A1 CA 3013052 A1 CA3013052 A1 CA 3013052A1 CA 3013052 A CA3013052 A CA 3013052A CA 3013052 A CA3013052 A CA 3013052A CA 3013052 A1 CA3013052 A1 CA 3013052A1
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- acoustic
- standing wave
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/04—Breaking emulsions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/06—Separation of liquids from each other by electricity
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/28—Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
- B01D21/283—Settling tanks provided with vibrators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Mechanical Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La présente invention concerne des procédés de séparation de billes et de cellules à partir d'un fluide hôte. Le procédé comprend l'écoulement d'un mélange contenant le fluide hôte, les billes et les cellules à travers un dispositif acoustophorétique comportant un transducteur ultrasonore comprenant un matériau piézoélectrique commandé par un signal de commande afin de créer une onde acoustique stationnaire multidimensionnelle. Un signal de commande est envoyé pour commander l'au moins un transducteur ultrasonore pour créer l'onde acoustique stationnaire multidimensionnelle. Un flux de fluide de recirculation ayant un trajet d'écoulement tangentiel est situé de façon sensiblement tangentielle à l'onde stationnaire et séparé de celle-ci par une région d'interface. Une partie des cellules traverse l'onde stationnaire, et les billes sont maintenues séparées de l'onde stationnaire dans le flux de fluide de recirculation au niveau de la région d'interface. L'invention concerne en outre un dispositif acoustophorétique comportant une entrée de réfrigérant conçue pour permettre l'entrée d'un fluide de refroidissement dans le dispositif pour refroidir le transducteur.
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662288500P | 2016-01-29 | 2016-01-29 | |
| US62/288,500 | 2016-01-29 | ||
| US201662296685P | 2016-02-18 | 2016-02-18 | |
| US62/296,685 | 2016-02-18 | ||
| US201662307934P | 2016-03-14 | 2016-03-14 | |
| US62/307,934 | 2016-03-14 | ||
| US15/139,187 US9752113B2 (en) | 2012-03-15 | 2016-04-26 | Acoustic perfusion devices |
| US15/139,187 | 2016-04-26 | ||
| PCT/US2017/015689 WO2017132694A1 (fr) | 2016-01-29 | 2017-01-30 | Dispositifs de perfusion acoustique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3013052A1 true CA3013052A1 (fr) | 2017-08-03 |
Family
ID=58228539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3013052A Pending CA3013052A1 (fr) | 2016-01-29 | 2017-01-30 | Dispositifs de perfusion acoustique |
Country Status (3)
| Country | Link |
|---|---|
| CA (1) | CA3013052A1 (fr) |
| GB (1) | GB2562937B (fr) |
| WO (1) | WO2017132694A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11474085B2 (en) | 2015-07-28 | 2022-10-18 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| US11459540B2 (en) | 2015-07-28 | 2022-10-04 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| WO2020232370A1 (fr) * | 2019-05-15 | 2020-11-19 | Flodesign Sonics, Inc. | Effet de bord acoustique |
| JP7567348B2 (ja) * | 2020-10-14 | 2024-10-16 | セイコーエプソン株式会社 | 流体デバイス |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003102737A2 (fr) * | 2002-06-04 | 2003-12-11 | Protasis Corporation | Dispositif et procede permettant de manipuler de maniere ultrasonique des particules transportees par un fluide |
| US20100206818A1 (en) * | 2009-02-19 | 2010-08-19 | Chartered Semiconductor Manufacturing, Ltd. | Ultrasonic filtration for cmp slurry |
| US9416344B2 (en) * | 2012-03-15 | 2016-08-16 | Flodesign Sonics, Inc. | Bioreactor using acoustic standing waves |
| WO2014046605A1 (fr) * | 2012-09-21 | 2014-03-27 | Acousort Ab | Procédé de séparation de complexes cellules-billes |
-
2017
- 2017-01-30 WO PCT/US2017/015689 patent/WO2017132694A1/fr active Application Filing
- 2017-01-30 CA CA3013052A patent/CA3013052A1/fr active Pending
- 2017-01-30 GB GB1812196.2A patent/GB2562937B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| GB2562937B (en) | 2022-01-05 |
| GB2562937A (en) | 2018-11-28 |
| WO2017132694A1 (fr) | 2017-08-03 |
| GB201812196D0 (en) | 2018-09-12 |
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