GB2562937A - Acoustic perfusion devices - Google Patents

Acoustic perfusion devices

Info

Publication number
GB2562937A
GB2562937A GB1812196.2A GB201812196A GB2562937A GB 2562937 A GB2562937 A GB 2562937A GB 201812196 A GB201812196 A GB 201812196A GB 2562937 A GB2562937 A GB 2562937A
Authority
GB
United Kingdom
Prior art keywords
standing wave
beads
cells
fluid
create
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1812196.2A
Other versions
GB2562937B (en
GB201812196D0 (en
Inventor
Lipkens Bart
M Presz Walter JR
Masi Louis
Ross-Johnsrud Benjamin
Miller Erik
J Kennedy Thomas Iii
Gilmanshin Rudolf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Flodesign Sonics Inc
Original Assignee
Flodesign Sonics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/139,187 external-priority patent/US9752113B2/en
Application filed by Flodesign Sonics Inc filed Critical Flodesign Sonics Inc
Publication of GB201812196D0 publication Critical patent/GB201812196D0/en
Publication of GB2562937A publication Critical patent/GB2562937A/en
Application granted granted Critical
Publication of GB2562937B publication Critical patent/GB2562937B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/04—Breaking emulsions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/06—Separation of liquids from each other by electricity
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/28—Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
    • B01D21/283—Settling tanks provided with vibrators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Methods are disclosed for separating beads and cells from a host fluid. The method includes flowing a mixture containing the host fluid, the beads, and the cells through an acoustophoretic device having an ultrasonic transducer including a piezoelectric material driven by a drive signal to create a multi-dimensional acoustic standing wave. A drive signal is sent to drive the at least one ultrasonic transducer to create the multi-dimensional acoustic standing wave. A recirculating fluid stream having a tangential flow path is located substantially tangential to the standing wave and separated therefrom by an interface region. A portion of the cells pass through the standing wave, and the beads are held back from the standing wave in the recirculating fluid stream at the interface region. Also disclosed is an acoustophoretic device having a coolant inlet adapted to permit the ingress of a cooling fluid into the device for cooling the transducer.
GB1812196.2A 2016-01-29 2017-01-30 Acoustic perfusion devices Active GB2562937B (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662288500P 2016-01-29 2016-01-29
US201662296685P 2016-02-18 2016-02-18
US201662307934P 2016-03-14 2016-03-14
US15/139,187 US9752113B2 (en) 2012-03-15 2016-04-26 Acoustic perfusion devices
PCT/US2017/015689 WO2017132694A1 (en) 2016-01-29 2017-01-30 Acoustic perfusion devices

Publications (3)

Publication Number Publication Date
GB201812196D0 GB201812196D0 (en) 2018-09-12
GB2562937A true GB2562937A (en) 2018-11-28
GB2562937B GB2562937B (en) 2022-01-05

Family

ID=58228539

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1812196.2A Active GB2562937B (en) 2016-01-29 2017-01-30 Acoustic perfusion devices

Country Status (3)

Country Link
CA (1) CA3013052A1 (en)
GB (1) GB2562937B (en)
WO (1) WO2017132694A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
BR112021021504A2 (en) * 2019-05-15 2021-12-21 Flodesign Sonics Inc Acoustic edge effect
JP2022064524A (en) * 2020-10-14 2022-04-26 セイコーエプソン株式会社 Fluid device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060037915A1 (en) * 2002-06-04 2006-02-23 Protasis Corporation Method and device for ultrasonically manipulating particles within a fluid
US20100206818A1 (en) * 2009-02-19 2010-08-19 Chartered Semiconductor Manufacturing, Ltd. Ultrasonic filtration for cmp slurry
WO2014046605A1 (en) * 2012-09-21 2014-03-27 Acousort Ab A method for separating cells-bead complexes
US20140154795A1 (en) * 2012-03-15 2014-06-05 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060037915A1 (en) * 2002-06-04 2006-02-23 Protasis Corporation Method and device for ultrasonically manipulating particles within a fluid
US20100206818A1 (en) * 2009-02-19 2010-08-19 Chartered Semiconductor Manufacturing, Ltd. Ultrasonic filtration for cmp slurry
US20140154795A1 (en) * 2012-03-15 2014-06-05 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
WO2014046605A1 (en) * 2012-09-21 2014-03-27 Acousort Ab A method for separating cells-bead complexes

Also Published As

Publication number Publication date
CA3013052A1 (en) 2017-08-03
GB2562937B (en) 2022-01-05
WO2017132694A1 (en) 2017-08-03
GB201812196D0 (en) 2018-09-12

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