CA2996535A1 - Bacteria engineered to treat disorders involving propionate catabolism - Google Patents
Bacteria engineered to treat disorders involving propionate catabolism Download PDFInfo
- Publication number
- CA2996535A1 CA2996535A1 CA2996535A CA2996535A CA2996535A1 CA 2996535 A1 CA2996535 A1 CA 2996535A1 CA 2996535 A CA2996535 A CA 2996535A CA 2996535 A CA2996535 A CA 2996535A CA 2996535 A1 CA2996535 A1 CA 2996535A1
- Authority
- CA
- Canada
- Prior art keywords
- propionate
- bacterium
- gene
- encoding
- promoter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 title claims abstract description 450
- 241000894006 Bacteria Species 0.000 title claims abstract description 400
- 230000015556 catabolic process Effects 0.000 title claims abstract description 263
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 731
- 230000001580 bacterial effect Effects 0.000 claims abstract description 220
- 108090000790 Enzymes Proteins 0.000 claims abstract description 217
- 102000004190 Enzymes Human genes 0.000 claims abstract description 211
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 86
- 201000004012 propionic acidemia Diseases 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 21
- 201000003694 methylmalonic acidemia Diseases 0.000 claims abstract description 21
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 10
- 210000004027 cell Anatomy 0.000 claims description 259
- 230000001939 inductive effect Effects 0.000 claims description 88
- 239000013612 plasmid Substances 0.000 claims description 83
- 239000001301 oxygen Substances 0.000 claims description 60
- 229910052760 oxygen Inorganic materials 0.000 claims description 60
- 201000010099 disease Diseases 0.000 claims description 55
- ZIYVHBGGAOATLY-UHFFFAOYSA-N methylmalonic acid Chemical compound OC(=O)C(C)C(O)=O ZIYVHBGGAOATLY-UHFFFAOYSA-N 0.000 claims description 53
- 230000007613 environmental effect Effects 0.000 claims description 52
- 241000588724 Escherichia coli Species 0.000 claims description 49
- 210000000349 chromosome Anatomy 0.000 claims description 43
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims description 32
- 108010078791 Carrier Proteins Proteins 0.000 claims description 29
- 238000012239 gene modification Methods 0.000 claims description 29
- 230000005017 genetic modification Effects 0.000 claims description 29
- 235000013617 genetically modified food Nutrition 0.000 claims description 29
- 208000035475 disorder Diseases 0.000 claims description 28
- 230000000694 effects Effects 0.000 claims description 27
- 101150036991 pccB gene Proteins 0.000 claims description 26
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 claims description 26
- 101150020468 prpE gene Proteins 0.000 claims description 25
- 229920000903 polyhydroxyalkanoate Polymers 0.000 claims description 23
- 101100155953 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) uvrD gene Proteins 0.000 claims description 19
- 101100132330 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) mutY gene Proteins 0.000 claims description 19
- 101150015240 mutB gene Proteins 0.000 claims description 19
- 101100297400 Rhizobium meliloti (strain 1021) phaAB gene Proteins 0.000 claims description 18
- 101150099430 accD5 gene Proteins 0.000 claims description 16
- 230000008676 import Effects 0.000 claims description 14
- 101150048611 phaC gene Proteins 0.000 claims description 13
- 241000606125 Bacteroides Species 0.000 claims description 12
- 239000006041 probiotic Substances 0.000 claims description 11
- 230000000529 probiotic effect Effects 0.000 claims description 11
- 235000018291 probiotics Nutrition 0.000 claims description 11
- 101100280476 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) fabM gene Proteins 0.000 claims description 10
- 101150110984 phaB gene Proteins 0.000 claims description 10
- 101100463818 Pseudomonas oleovorans phaC1 gene Proteins 0.000 claims description 8
- 101150046540 phaA gene Proteins 0.000 claims description 8
- 241000186000 Bifidobacterium Species 0.000 claims description 7
- 241000193403 Clostridium Species 0.000 claims description 7
- 241000588722 Escherichia Species 0.000 claims description 7
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 6
- 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 6
- 101150006248 dcuC gene Proteins 0.000 claims description 6
- 108091026922 FnrS RNA Proteins 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 231100000331 toxic Toxicity 0.000 claims description 5
- 230000002588 toxic effect Effects 0.000 claims description 5
- 241000194036 Lactococcus Species 0.000 claims description 4
- 101150029104 prpC gene Proteins 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 101100345719 Bacillus subtilis (strain 168) mmgE gene Proteins 0.000 claims description 3
- 101100345721 Bacillus subtilis (strain 168) mmgF gene Proteins 0.000 claims description 3
- 101150079081 pphB gene Proteins 0.000 claims description 3
- 101150085802 prpB gene Proteins 0.000 claims description 3
- 101150077061 prpD gene Proteins 0.000 claims description 3
- 239000006227 byproduct Substances 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims description 2
- 208000002670 vitamin B12 deficiency Diseases 0.000 claims description 2
- 201000008152 organic acidemia Diseases 0.000 claims 3
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 2
- GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 claims 1
- GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 claims 1
- 229940113082 thymine Drugs 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 description 250
- 229940088598 enzyme Drugs 0.000 description 187
- 108091023040 Transcription factor Proteins 0.000 description 162
- 102000040945 Transcription factor Human genes 0.000 description 162
- 230000014509 gene expression Effects 0.000 description 157
- 108090000765 processed proteins & peptides Proteins 0.000 description 102
- 101001102778 Escherichia coli (strain K12) Sodium/proline symporter Proteins 0.000 description 86
- 239000003642 reactive oxygen metabolite Substances 0.000 description 85
- MZFOKIKEPGUZEN-FBMOWMAESA-N methylmalonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C(C(O)=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MZFOKIKEPGUZEN-FBMOWMAESA-N 0.000 description 79
- QAQREVBBADEHPA-IEXPHMLFSA-N propionyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QAQREVBBADEHPA-IEXPHMLFSA-N 0.000 description 78
- 108091008324 binding proteins Proteins 0.000 description 77
- 239000007845 reactive nitrogen species Substances 0.000 description 73
- 102000004196 processed proteins & peptides Human genes 0.000 description 72
- 102000004169 proteins and genes Human genes 0.000 description 72
- 235000018102 proteins Nutrition 0.000 description 66
- 102000014914 Carrier Proteins Human genes 0.000 description 62
- 229920001184 polypeptide Polymers 0.000 description 62
- 230000027455 binding Effects 0.000 description 60
- 230000037361 pathway Effects 0.000 description 60
- 235000001014 amino acid Nutrition 0.000 description 54
- 229940024606 amino acid Drugs 0.000 description 51
- 150000001413 amino acids Chemical class 0.000 description 51
- 239000002207 metabolite Substances 0.000 description 51
- 210000001035 gastrointestinal tract Anatomy 0.000 description 44
- 150000007523 nucleic acids Chemical group 0.000 description 41
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 37
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 37
- 230000001225 therapeutic effect Effects 0.000 description 36
- 108091028043 Nucleic acid sequence Proteins 0.000 description 35
- 244000005700 microbiome Species 0.000 description 30
- 108700012359 toxins Proteins 0.000 description 30
- 230000028327 secretion Effects 0.000 description 29
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 28
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 28
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 28
- 230000001419 dependent effect Effects 0.000 description 28
- 239000003053 toxin Substances 0.000 description 27
- 231100000765 toxin Toxicity 0.000 description 27
- 238000013518 transcription Methods 0.000 description 27
- 230000035897 transcription Effects 0.000 description 27
- 230000002103 transcriptional effect Effects 0.000 description 27
- -1 Methylmalonyl Chemical group 0.000 description 26
- 108091026890 Coding region Proteins 0.000 description 25
- 108700020534 tetracycline resistance-encoding transposon repressor Proteins 0.000 description 25
- 108010085747 Methylmalonyl-CoA Decarboxylase Proteins 0.000 description 24
- 230000001147 anti-toxic effect Effects 0.000 description 23
- 239000002773 nucleotide Substances 0.000 description 22
- 125000003729 nucleotide group Chemical group 0.000 description 22
- 230000035772 mutation Effects 0.000 description 21
- YNOXCRMFGMSKIJ-NFNCENRGSA-N (2S,3S)-2-methylcitric acid Chemical compound OC(=O)[C@@H](C)[C@](O)(C(O)=O)CC(O)=O YNOXCRMFGMSKIJ-NFNCENRGSA-N 0.000 description 20
- 125000003275 alpha amino acid group Chemical group 0.000 description 19
- 102000018120 Recombinases Human genes 0.000 description 18
- 108010091086 Recombinases Proteins 0.000 description 18
- 101100510642 Streptococcus mutans lanA gene Proteins 0.000 description 17
- 230000007423 decrease Effects 0.000 description 17
- 239000012634 fragment Substances 0.000 description 17
- 101150079289 mutA gene Proteins 0.000 description 17
- 230000002068 genetic effect Effects 0.000 description 16
- 235000019260 propionic acid Nutrition 0.000 description 16
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 16
- 230000037430 deletion Effects 0.000 description 15
- 238000012217 deletion Methods 0.000 description 15
- 230000006870 function Effects 0.000 description 15
- 230000000670 limiting effect Effects 0.000 description 15
- 241000894007 species Species 0.000 description 15
- 210000003578 bacterial chromosome Anatomy 0.000 description 14
- 241000124008 Mammalia Species 0.000 description 13
- 230000008859 change Effects 0.000 description 13
- 239000012528 membrane Substances 0.000 description 13
- 108020004999 messenger RNA Proteins 0.000 description 13
- 230000004048 modification Effects 0.000 description 13
- 238000012986 modification Methods 0.000 description 13
- 238000011282 treatment Methods 0.000 description 13
- 230000004913 activation Effects 0.000 description 12
- 244000052616 bacterial pathogen Species 0.000 description 12
- 230000037431 insertion Effects 0.000 description 12
- 238000003780 insertion Methods 0.000 description 12
- 210000000813 small intestine Anatomy 0.000 description 12
- VNOYUJKHFWYWIR-ITIYDSSPSA-N succinyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VNOYUJKHFWYWIR-ITIYDSSPSA-N 0.000 description 12
- 108020004705 Codon Proteins 0.000 description 11
- 101100028324 Streptomyces rimosus subsp. rimosus (strain ATCC 10970 / DSM 40260 / JCM 4667 / NRRL 2234) oxyS gene Proteins 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 11
- 238000013461 design Methods 0.000 description 11
- 230000012010 growth Effects 0.000 description 11
- 238000001727 in vivo Methods 0.000 description 11
- 230000002779 inactivation Effects 0.000 description 11
- 230000001965 increasing effect Effects 0.000 description 11
- 108700039887 Essential Genes Proteins 0.000 description 10
- 241000700605 Viruses Species 0.000 description 10
- 230000003213 activating effect Effects 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 239000011541 reaction mixture Substances 0.000 description 10
- 238000006467 substitution reaction Methods 0.000 description 10
- 208000024891 symptom Diseases 0.000 description 10
- 238000013519 translation Methods 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 240000006024 Lactobacillus plantarum Species 0.000 description 9
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 9
- 102000019010 Methylmalonyl-CoA Mutase Human genes 0.000 description 9
- 108010051862 Methylmalonyl-CoA mutase Proteins 0.000 description 9
- 239000004098 Tetracycline Substances 0.000 description 9
- 229940072205 lactobacillus plantarum Drugs 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 229960002180 tetracycline Drugs 0.000 description 9
- 229930101283 tetracycline Natural products 0.000 description 9
- 235000019364 tetracycline Nutrition 0.000 description 9
- 150000003522 tetracyclines Chemical class 0.000 description 9
- 238000011144 upstream manufacturing Methods 0.000 description 9
- 108010048816 AraC Transcription Factor Proteins 0.000 description 8
- 101100162202 Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1) aflF gene Proteins 0.000 description 8
- 241000193171 Clostridium butyricum Species 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 8
- 240000001046 Lactobacillus acidophilus Species 0.000 description 8
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 8
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 8
- 230000002159 abnormal effect Effects 0.000 description 8
- 230000003115 biocidal effect Effects 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000002503 metabolic effect Effects 0.000 description 8
- 239000006151 minimal media Substances 0.000 description 8
- 101150076456 norB gene Proteins 0.000 description 8
- 108010033058 propionate - CoA ligase Proteins 0.000 description 8
- 230000002829 reductive effect Effects 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 230000004083 survival effect Effects 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 7
- 241000606124 Bacteroides fragilis Species 0.000 description 7
- 241000186015 Bifidobacterium longum subsp. infantis Species 0.000 description 7
- 241000282412 Homo Species 0.000 description 7
- 241000186604 Lactobacillus reuteri Species 0.000 description 7
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 7
- 239000004472 Lysine Substances 0.000 description 7
- 244000057717 Streptococcus lactis Species 0.000 description 7
- 235000014897 Streptococcus lactis Nutrition 0.000 description 7
- 101150046124 accA gene Proteins 0.000 description 7
- 229940004120 bifidobacterium infantis Drugs 0.000 description 7
- 238000004113 cell culture Methods 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 108020001507 fusion proteins Proteins 0.000 description 7
- 102000037865 fusion proteins Human genes 0.000 description 7
- 239000000411 inducer Substances 0.000 description 7
- 101150013110 katG gene Proteins 0.000 description 7
- 229940001882 lactobacillus reuteri Drugs 0.000 description 7
- 230000007246 mechanism Effects 0.000 description 7
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 7
- MZFOKIKEPGUZEN-AGCMQPJKSA-N (R)-methylmalonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)[C@@H](C(O)=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MZFOKIKEPGUZEN-AGCMQPJKSA-N 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- 244000063299 Bacillus subtilis Species 0.000 description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 description 6
- 241000606123 Bacteroides thetaiotaomicron Species 0.000 description 6
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 6
- 241000186016 Bifidobacterium bifidum Species 0.000 description 6
- 101100135734 Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) pccB gene Proteins 0.000 description 6
- 101150008263 accD gene Proteins 0.000 description 6
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 6
- 238000007792 addition Methods 0.000 description 6
- 229940002008 bifidobacterium bifidum Drugs 0.000 description 6
- 229940009289 bifidobacterium lactis Drugs 0.000 description 6
- 230000001925 catabolic effect Effects 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 230000002759 chromosomal effect Effects 0.000 description 6
- 210000002429 large intestine Anatomy 0.000 description 6
- 210000004185 liver Anatomy 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 230000008520 organization Effects 0.000 description 6
- 238000003753 real-time PCR Methods 0.000 description 6
- 230000004044 response Effects 0.000 description 6
- 241000023308 Acca Species 0.000 description 5
- 101100295756 Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81) omp38 gene Proteins 0.000 description 5
- 102000057234 Acyl transferases Human genes 0.000 description 5
- 108700016155 Acyl transferases Proteins 0.000 description 5
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 5
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 5
- 108700010070 Codon Usage Proteins 0.000 description 5
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 5
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 5
- 102000003960 Ligases Human genes 0.000 description 5
- 108090000364 Ligases Proteins 0.000 description 5
- 101100178822 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) htrA1 gene Proteins 0.000 description 5
- 108090000913 Nitrate Reductases Proteins 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 5
- 108010076504 Protein Sorting Signals Proteins 0.000 description 5
- 101100277437 Rhizobium meliloti (strain 1021) degP1 gene Proteins 0.000 description 5
- 241000607142 Salmonella Species 0.000 description 5
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 5
- 101150042295 arfA gene Proteins 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 239000000090 biomarker Substances 0.000 description 5
- 210000004556 brain Anatomy 0.000 description 5
- 210000001072 colon Anatomy 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 5
- 101150018266 degP gene Proteins 0.000 description 5
- 101150083941 degS gene Proteins 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- LTYOQGRJFJAKNA-IJCONWDESA-N malonyl-coenzyme a Chemical compound O[C@@H]1[C@@H](OP(O)(O)=O)[C@H](CO[P@](O)(=O)O[P@@](O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LTYOQGRJFJAKNA-IJCONWDESA-N 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 101150087557 omcB gene Proteins 0.000 description 5
- 101150115693 ompA gene Proteins 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 230000008707 rearrangement Effects 0.000 description 5
- 230000000754 repressing effect Effects 0.000 description 5
- 230000002441 reversible effect Effects 0.000 description 5
- 229960002181 saccharomyces boulardii Drugs 0.000 description 5
- 230000003248 secreting effect Effects 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 5
- MZFOKIKEPGUZEN-IBNUZSNCSA-N (S)-methylmalonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)[C@H](C(O)=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MZFOKIKEPGUZEN-IBNUZSNCSA-N 0.000 description 4
- 101100427060 Bacillus spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) thyA1 gene Proteins 0.000 description 4
- 101100462127 Bacillus subtilis (strain 168) ohrA gene Proteins 0.000 description 4
- 101100203377 Bacillus subtilis (strain 168) slp gene Proteins 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241000186226 Corynebacterium glutamicum Species 0.000 description 4
- 101100242529 Drosophila melanogaster Pal2 gene Proteins 0.000 description 4
- 241000194031 Enterococcus faecium Species 0.000 description 4
- 101100153154 Escherichia phage T5 thy gene Proteins 0.000 description 4
- 241000192125 Firmicutes Species 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 206010061218 Inflammation Diseases 0.000 description 4
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 4
- 101150012565 LRIT1 gene Proteins 0.000 description 4
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 4
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 4
- 241000186605 Lactobacillus paracasei Species 0.000 description 4
- 101100477497 Mus musculus Shcbp1 gene Proteins 0.000 description 4
- 101100509674 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) katG3 gene Proteins 0.000 description 4
- 241000350158 Prioria balsamifera Species 0.000 description 4
- 241000190950 Rhodopseudomonas palustris Species 0.000 description 4
- 101100313751 Rickettsia conorii (strain ATCC VR-613 / Malish 7) thyX gene Proteins 0.000 description 4
- 241000235070 Saccharomyces Species 0.000 description 4
- 229930003779 Vitamin B12 Natural products 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 101150024831 ahpC gene Proteins 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 230000008238 biochemical pathway Effects 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 210000004534 cecum Anatomy 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 210000003238 esophagus Anatomy 0.000 description 4
- 235000021472 generally recognized as safe Nutrition 0.000 description 4
- 210000004907 gland Anatomy 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 101150066555 lacZ gene Proteins 0.000 description 4
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 4
- 238000004949 mass spectrometry Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 101150029849 nsrR gene Proteins 0.000 description 4
- 101150073640 ompF gene Proteins 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 230000001590 oxidative effect Effects 0.000 description 4
- 101150077062 pal gene Proteins 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 229920000218 poly(hydroxyvalerate) Polymers 0.000 description 4
- 238000012809 post-inoculation Methods 0.000 description 4
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 4
- 239000004324 sodium propionate Substances 0.000 description 4
- 235000010334 sodium propionate Nutrition 0.000 description 4
- 229960003212 sodium propionate Drugs 0.000 description 4
- 101150033650 soxS gene Proteins 0.000 description 4
- 230000006641 stabilisation Effects 0.000 description 4
- 238000011105 stabilization Methods 0.000 description 4
- 210000002784 stomach Anatomy 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 101150072314 thyA gene Proteins 0.000 description 4
- 101150061964 tolA gene Proteins 0.000 description 4
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 4
- 235000019163 vitamin B12 Nutrition 0.000 description 4
- 239000011715 vitamin B12 Substances 0.000 description 4
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 3
- 102100026024 Acyl-coenzyme A synthetase ACSM3, mitochondrial Human genes 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 241000193749 Bacillus coagulans Species 0.000 description 3
- 241001608472 Bifidobacterium longum Species 0.000 description 3
- 101100465553 Dictyostelium discoideum psmB6 gene Proteins 0.000 description 3
- 241000194033 Enterococcus Species 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- 102100024977 Glutamine-tRNA ligase Human genes 0.000 description 3
- 241001026509 Kata Species 0.000 description 3
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 3
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 3
- 244000199866 Lactobacillus casei Species 0.000 description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 description 3
- 241001468157 Lactobacillus johnsonii Species 0.000 description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 3
- 108020000282 Methylisocitrate lyase Proteins 0.000 description 3
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 3
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 3
- 241000605936 Oxalobacter formigenes Species 0.000 description 3
- 108010013639 Peptidoglycan Proteins 0.000 description 3
- 229920000331 Polyhydroxybutyrate Polymers 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 101100169519 Pyrococcus abyssi (strain GE5 / Orsay) dapAL gene Proteins 0.000 description 3
- 241000191940 Staphylococcus Species 0.000 description 3
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 3
- 108020004566 Transfer RNA Proteins 0.000 description 3
- 108010069584 Type III Secretion Systems Proteins 0.000 description 3
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 108010069175 acyl-CoA transferase Proteins 0.000 description 3
- 101150044616 araC gene Proteins 0.000 description 3
- 229940054340 bacillus coagulans Drugs 0.000 description 3
- 229940009291 bifidobacterium longum Drugs 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 150000005693 branched-chain amino acids Chemical class 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 3
- 230000030833 cell death Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 210000000172 cytosol Anatomy 0.000 description 3
- 101150011371 dapA gene Proteins 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 229920003045 dextran sodium sulfate Polymers 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- LZDSILRDTDCIQT-UHFFFAOYSA-N dinitrogen trioxide Chemical compound [O-][N+](=O)N=O LZDSILRDTDCIQT-UHFFFAOYSA-N 0.000 description 3
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 3
- 235000011180 diphosphates Nutrition 0.000 description 3
- 239000002158 endotoxin Substances 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 230000005714 functional activity Effects 0.000 description 3
- 108010051239 glutaminyl-tRNA synthetase Proteins 0.000 description 3
- 239000003228 hemolysin Substances 0.000 description 3
- 101150090497 ilvC gene Proteins 0.000 description 3
- 230000004968 inflammatory condition Effects 0.000 description 3
- 230000002757 inflammatory effect Effects 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 230000028709 inflammatory response Effects 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 229960000310 isoleucine Drugs 0.000 description 3
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 230000002147 killing effect Effects 0.000 description 3
- 229940017800 lactobacillus casei Drugs 0.000 description 3
- 229920006008 lipopolysaccharide Polymers 0.000 description 3
- 210000003750 lower gastrointestinal tract Anatomy 0.000 description 3
- 101150086633 metAA gene Proteins 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 101150114893 oxyR gene Proteins 0.000 description 3
- 230000007918 pathogenicity Effects 0.000 description 3
- 150000002978 peroxides Chemical class 0.000 description 3
- CMFNMSMUKZHDEY-UHFFFAOYSA-N peroxynitrous acid Chemical compound OON=O CMFNMSMUKZHDEY-UHFFFAOYSA-N 0.000 description 3
- 239000005015 poly(hydroxybutyrate) Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 description 3
- 210000003705 ribosome Anatomy 0.000 description 3
- WIOQNWTZBOQTEU-ZMHDXICWSA-N s-[2-[3-[[(2r)-4-[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] 3-oxopentanethioate Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)CC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 WIOQNWTZBOQTEU-ZMHDXICWSA-N 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 230000019491 signal transduction Effects 0.000 description 3
- 125000003003 spiro group Chemical group 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 108091006106 transcriptional activators Proteins 0.000 description 3
- 108091006107 transcriptional repressors Proteins 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- 230000001960 triggered effect Effects 0.000 description 3
- 210000002438 upper gastrointestinal tract Anatomy 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 239000004474 valine Substances 0.000 description 3
- 101150096946 ydfZ gene Proteins 0.000 description 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 2
- YUTUUOJFXIMELV-UHFFFAOYSA-N 2-Hydroxy-2-(2-methoxy-2-oxoethyl)butanedioic acid Chemical compound COC(=O)CC(O)(C(O)=O)CC(O)=O YUTUUOJFXIMELV-UHFFFAOYSA-N 0.000 description 2
- QHHKKMYHDBRONY-RMNRSTNRSA-N 3-hydroxybutanoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QHHKKMYHDBRONY-RMNRSTNRSA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 description 2
- 241000589291 Acinetobacter Species 0.000 description 2
- 101100059196 Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) katE gene Proteins 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 2
- 241001233887 Ania Species 0.000 description 2
- 101100290837 Bacillus subtilis (strain 168) metAA gene Proteins 0.000 description 2
- 101100076641 Bacillus subtilis (strain 168) metE gene Proteins 0.000 description 2
- 101100402524 Bacillus subtilis (strain 168) mrgA gene Proteins 0.000 description 2
- 101100197953 Bacillus subtilis (strain 168) rlbA gene Proteins 0.000 description 2
- 241001134770 Bifidobacterium animalis Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- HCVBQXINVUFVCE-UHFFFAOYSA-N Citronensaeure-beta-methylester Natural products COC(=O)C(O)(CC(O)=O)CC(O)=O HCVBQXINVUFVCE-UHFFFAOYSA-N 0.000 description 2
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 102100029133 DNA damage-induced apoptosis suppressor protein Human genes 0.000 description 2
- 101710088194 Dehydrogenase Proteins 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 101100498063 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) cysB gene Proteins 0.000 description 2
- 241000588921 Enterobacteriaceae Species 0.000 description 2
- 101001005515 Escherichia coli (strain K12) Peptidyl-lysine N-acetyltransferase PatZ Proteins 0.000 description 2
- 101100390720 Escherichia coli (strain K12) fhuF gene Proteins 0.000 description 2
- 101100126128 Escherichia coli (strain K12) insE6 gene Proteins 0.000 description 2
- 241001302654 Escherichia coli Nissle 1917 Species 0.000 description 2
- 101100082540 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) pcp gene Proteins 0.000 description 2
- 101000918646 Homo sapiens DNA damage-induced apoptosis suppressor protein Proteins 0.000 description 2
- 101000991619 Homo sapiens Meprin A subunit alpha Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 208000002720 Malnutrition Diseases 0.000 description 2
- 102100025169 Max-binding protein MNT Human genes 0.000 description 2
- 102100030882 Meprin A subunit alpha Human genes 0.000 description 2
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 2
- 101100276922 Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) dapF2 gene Proteins 0.000 description 2
- RDHQFKQIGNGIED-MRVPVSSYSA-O O-acetylcarnitinium Chemical compound CC(=O)O[C@H](CC(O)=O)C[N+](C)(C)C RDHQFKQIGNGIED-MRVPVSSYSA-O 0.000 description 2
- UFAHZIUFPNSHSL-UHFFFAOYSA-N O-propanoylcarnitine Chemical compound CCC(=O)OC(CC([O-])=O)C[N+](C)(C)C UFAHZIUFPNSHSL-UHFFFAOYSA-N 0.000 description 2
- 101710116435 Outer membrane protein Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 241000186428 Propionibacterium freudenreichii Species 0.000 description 2
- 108700008625 Reporter Genes Proteins 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 101100091507 Rhizobium etli (strain CFN 42 / ATCC 51251) rosR gene Proteins 0.000 description 2
- 101001005516 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Peptidyl-lysine N-acetyltransferase Pat Proteins 0.000 description 2
- 241000187432 Streptomyces coelicolor Species 0.000 description 2
- 101100125907 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) ilvC1 gene Proteins 0.000 description 2
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 2
- 101100116197 Streptomyces lavendulae dcsC gene Proteins 0.000 description 2
- 101100329526 Synechocystis sp. (strain PCC 6803 / Kazusa) csd gene Proteins 0.000 description 2
- 101100245038 Synechocystis sp. (strain PCC 6803 / Kazusa) proA1 gene Proteins 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- 108010073429 Type V Secretion Systems Proteins 0.000 description 2
- 108010046179 Type VI Secretion Systems Proteins 0.000 description 2
- 241001147801 [Clostridium] scindens Species 0.000 description 2
- 101150057540 aar gene Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- OJFDKHTZOUZBOS-CITAKDKDSA-N acetoacetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 OJFDKHTZOUZBOS-CITAKDKDSA-N 0.000 description 2
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 2
- 229960001009 acetylcarnitine Drugs 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 101150061301 ahpF gene Proteins 0.000 description 2
- 230000001668 ameliorated effect Effects 0.000 description 2
- 210000002255 anal canal Anatomy 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 210000000436 anus Anatomy 0.000 description 2
- 108700003859 araC Genes Proteins 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 229940118852 bifidobacterium animalis Drugs 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 239000011436 cob Substances 0.000 description 2
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 2
- 239000005516 coenzyme A Substances 0.000 description 2
- 229940093530 coenzyme a Drugs 0.000 description 2
- 238000007398 colorimetric assay Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 101150000386 cpfC gene Proteins 0.000 description 2
- 101150111114 cysE gene Proteins 0.000 description 2
- 231100000599 cytotoxic agent Toxicity 0.000 description 2
- 239000002619 cytotoxin Substances 0.000 description 2
- 101150073654 dapB gene Proteins 0.000 description 2
- 101150064923 dapD gene Proteins 0.000 description 2
- 101150000582 dapE gene Proteins 0.000 description 2
- 101150062988 dapF gene Proteins 0.000 description 2
- 230000002939 deleterious effect Effects 0.000 description 2
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000000539 dimer Substances 0.000 description 2
- 101150026863 dsbG gene Proteins 0.000 description 2
- 210000001198 duodenum Anatomy 0.000 description 2
- 101150046913 ecpA gene Proteins 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 239000000147 enterotoxin Substances 0.000 description 2
- 231100000655 enterotoxin Toxicity 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 210000001723 extracellular space Anatomy 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 101150060377 flhD gene Proteins 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 210000000232 gallbladder Anatomy 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 101150097303 glyA gene Proteins 0.000 description 2
- 101150079604 glyA1 gene Proteins 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 101150002138 grxA gene Proteins 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 101150042363 hemH gene Proteins 0.000 description 2
- 101150107671 hisB gene Proteins 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 2
- 210000003405 ileum Anatomy 0.000 description 2
- 101150095957 ilvA gene Proteins 0.000 description 2
- 101150043028 ilvD gene Proteins 0.000 description 2
- 101150105723 ilvD1 gene Proteins 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000002427 irreversible effect Effects 0.000 description 2
- 230000000155 isotopic effect Effects 0.000 description 2
- 210000001630 jejunum Anatomy 0.000 description 2
- 101150115163 katA gene Proteins 0.000 description 2
- 101150108683 katG2 gene Proteins 0.000 description 2
- 101150025049 leuB gene Proteins 0.000 description 2
- WQVJUBFKFCDYDQ-BBWFWOEESA-N leubethanol Natural products C1=C(C)C=C2[C@H]([C@H](CCC=C(C)C)C)CC[C@@H](C)C2=C1O WQVJUBFKFCDYDQ-BBWFWOEESA-N 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 101150074251 lpp gene Proteins 0.000 description 2
- 101150033534 lysA gene Proteins 0.000 description 2
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 2
- 230000002132 lysosomal effect Effects 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 101150060102 metA gene Proteins 0.000 description 2
- 101150091110 metAS gene Proteins 0.000 description 2
- 101150003180 metB gene Proteins 0.000 description 2
- 101150117293 metC gene Proteins 0.000 description 2
- 101150043924 metXA gene Proteins 0.000 description 2
- 230000037353 metabolic pathway Effects 0.000 description 2
- 239000013264 metal-organic assembly Substances 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 101150055934 mgrA gene Proteins 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 101150002988 norR gene Proteins 0.000 description 2
- 235000018343 nutrient deficiency Nutrition 0.000 description 2
- 230000009437 off-target effect Effects 0.000 description 2
- 206010073131 oligoastrocytoma Diseases 0.000 description 2
- 101150110245 ompC gene Proteins 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 101150023849 pheA gene Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 230000004481 post-translational protein modification Effects 0.000 description 2
- 239000013615 primer Substances 0.000 description 2
- 101150118057 proA gene Proteins 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000006289 propionylation Effects 0.000 description 2
- 238000010515 propionylation reaction Methods 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 101150099879 prpR gene Proteins 0.000 description 2
- 101150116440 pyrF gene Proteins 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 210000000664 rectum Anatomy 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000003938 response to stress Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000000979 retarding effect Effects 0.000 description 2
- YYGYPCRWZMLSGK-XMWLYHNJSA-N s-[2-[3-[[(2r)-4-[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] 3-hydroxypentanethioate Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)CC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 YYGYPCRWZMLSGK-XMWLYHNJSA-N 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 101150118796 sufS gene Proteins 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 101150061166 tetR gene Proteins 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 150000007970 thio esters Chemical class 0.000 description 2
- 101150000850 thrC gene Proteins 0.000 description 2
- 108091008023 transcriptional regulators Proteins 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 101150016309 trpC gene Proteins 0.000 description 2
- 101150118060 trxA gene Proteins 0.000 description 2
- 101150112435 trxC gene Proteins 0.000 description 2
- 101150046435 uraA gene Proteins 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 239000000304 virulence factor Substances 0.000 description 2
- 230000007923 virulence factor Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 101150024223 yaaA gene Proteins 0.000 description 2
- PHIQHXFUZVPYII-ZCFIWIBFSA-N (R)-carnitine Chemical compound C[N+](C)(C)C[C@H](O)CC([O-])=O PHIQHXFUZVPYII-ZCFIWIBFSA-N 0.000 description 1
- BCOSEZGCLGPUSL-UHFFFAOYSA-N 2,3,3-trichloroprop-2-enoyl chloride Chemical compound ClC(Cl)=C(Cl)C(Cl)=O BCOSEZGCLGPUSL-UHFFFAOYSA-N 0.000 description 1
- JTTIOYHBNXDJOD-UHFFFAOYSA-N 2,4,6-triaminopyrimidine Chemical compound NC1=CC(N)=NC(N)=N1 JTTIOYHBNXDJOD-UHFFFAOYSA-N 0.000 description 1
- 108010030844 2-methylcitrate synthase Proteins 0.000 description 1
- 102100026105 3-ketoacyl-CoA thiolase, mitochondrial Human genes 0.000 description 1
- MGWGWNFMUOTEHG-UHFFFAOYSA-N 4-(3,5-dimethylphenyl)-1,3-thiazol-2-amine Chemical compound CC1=CC(C)=CC(C=2N=C(N)SC=2)=C1 MGWGWNFMUOTEHG-UHFFFAOYSA-N 0.000 description 1
- YBJHBAHKTGYVGT-OOZYFLPDSA-N 5-[(3as,4r,6ar)-2-oxohexahydro-1h-thieno[3,4-d]imidazol-4-yl]pentanoic acid Chemical compound N1C(=O)N[C@@H]2[C@@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-OOZYFLPDSA-N 0.000 description 1
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical compound NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 1
- 102100030840 AT-rich interactive domain-containing protein 4B Human genes 0.000 description 1
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 description 1
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 description 1
- 102000004672 Acetyl-CoA C-acyltransferase Human genes 0.000 description 1
- 108010016219 Acetyl-CoA carboxylase Proteins 0.000 description 1
- 208000013824 Acidemia Diseases 0.000 description 1
- 208000010444 Acidosis Diseases 0.000 description 1
- 102000034263 Amino acid transporters Human genes 0.000 description 1
- 108050005273 Amino acid transporters Proteins 0.000 description 1
- 101710109637 Antigen 43 Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 101100480489 Arabidopsis thaliana TAAC gene Proteins 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 101100002068 Bacillus subtilis (strain 168) araR gene Proteins 0.000 description 1
- 101100326957 Bacillus subtilis (strain 168) catD gene Proteins 0.000 description 1
- 101100404144 Bacillus subtilis (strain 168) nasD gene Proteins 0.000 description 1
- 101100478574 Bacillus subtilis (strain 168) ssuA gene Proteins 0.000 description 1
- 101100213426 Bacillus subtilis (strain 168) yhbB gene Proteins 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- 241000186018 Bifidobacterium adolescentis Species 0.000 description 1
- 208000014644 Brain disease Diseases 0.000 description 1
- 241000863012 Caulobacter Species 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- 241000193401 Clostridium acetobutylicum Species 0.000 description 1
- 241000193167 Clostridium cochlearium Species 0.000 description 1
- 241001509499 Clostridium felsineum Species 0.000 description 1
- 241000530936 Clostridium novyi NT Species 0.000 description 1
- 241001147791 Clostridium paraputrificum Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- 241001656801 Clostridium roseum Species 0.000 description 1
- 241000193470 Clostridium sporogenes Species 0.000 description 1
- 241000186528 Clostridium tertium Species 0.000 description 1
- 241000193449 Clostridium tetani Species 0.000 description 1
- 241000193452 Clostridium tyrobutyricum Species 0.000 description 1
- 102000005870 Coenzyme A Ligases Human genes 0.000 description 1
- 108010073254 Colicins Proteins 0.000 description 1
- 206010009900 Colitis ulcerative Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 208000011231 Crohn disease Diseases 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 238000007702 DNA assembly Methods 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 101710096438 DNA-binding protein Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 206010012559 Developmental delay Diseases 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 208000032274 Encephalopathy Diseases 0.000 description 1
- 241000701832 Enterobacteria phage T3 Species 0.000 description 1
- 101100496313 Escherichia coli (strain K12) clpS gene Proteins 0.000 description 1
- 101100337717 Escherichia coli (strain K12) grcA gene Proteins 0.000 description 1
- 101100213349 Escherichia coli (strain K12) ygbA gene Proteins 0.000 description 1
- 241000660147 Escherichia coli str. K-12 substr. MG1655 Species 0.000 description 1
- 108010014531 FMN Reductase Proteins 0.000 description 1
- 101150099538 FNR gene Proteins 0.000 description 1
- 238000011771 FVB mouse Methods 0.000 description 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical class [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 description 1
- 108010057573 Flavoproteins Proteins 0.000 description 1
- 102000003983 Flavoproteins Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 102000005720 Glutathione transferase Human genes 0.000 description 1
- 108010070675 Glutathione transferase Proteins 0.000 description 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- 101100293613 Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) nasB gene Proteins 0.000 description 1
- 241000193159 Hathewaya histolytica Species 0.000 description 1
- 108010006464 Hemolysin Proteins Proteins 0.000 description 1
- 108010072039 Histidine kinase Proteins 0.000 description 1
- 101000792935 Homo sapiens AT-rich interactive domain-containing protein 4B Proteins 0.000 description 1
- 101000994204 Homo sapiens D-ribitol-5-phosphate cytidylyltransferase Proteins 0.000 description 1
- 101000724418 Homo sapiens Neutral amino acid transporter B(0) Proteins 0.000 description 1
- 108090001042 Hydro-Lyases Proteins 0.000 description 1
- 102000004867 Hydro-Lyases Human genes 0.000 description 1
- 206010020575 Hyperammonaemia Diseases 0.000 description 1
- 206010021118 Hypotonia Diseases 0.000 description 1
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 108010035210 Iron-Binding Proteins Proteins 0.000 description 1
- 102000008133 Iron-Binding Proteins Human genes 0.000 description 1
- 101100140190 Klebsiella pneumoniae rbtT gene Proteins 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 125000000174 L-prolyl group Chemical group [H]N1C([H])([H])C([H])([H])C([H])([H])[C@@]1([H])C(*)=O 0.000 description 1
- 125000000510 L-tryptophano group Chemical group [H]C1=C([H])C([H])=C2N([H])C([H])=C(C([H])([H])[C@@]([H])(C(O[H])=O)N([H])[*])C2=C1[H] 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 241000589248 Legionella Species 0.000 description 1
- 208000007764 Legionnaires' Disease Diseases 0.000 description 1
- 206010024264 Lethargy Diseases 0.000 description 1
- 241000186781 Listeria Species 0.000 description 1
- 241000186779 Listeria monocytogenes Species 0.000 description 1
- 108010011449 Long-chain-fatty-acid-CoA ligase Proteins 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-L Malonate Chemical compound [O-]C(=O)CC([O-])=O OFOBLEOULBTSOW-UHFFFAOYSA-L 0.000 description 1
- 102000018897 Membrane Fusion Proteins Human genes 0.000 description 1
- 108010027796 Membrane Fusion Proteins Proteins 0.000 description 1
- 208000036626 Mental retardation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010059521 Methylmalonic aciduria Diseases 0.000 description 1
- 102000030503 Methylmalonyl-CoA epimerase Human genes 0.000 description 1
- 241000736262 Microbiota Species 0.000 description 1
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
- 108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 101100420202 Mus musculus Rpusd1 gene Proteins 0.000 description 1
- 208000007379 Muscle Hypotonia Diseases 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 241000186366 Mycobacterium bovis Species 0.000 description 1
- 241000588653 Neisseria Species 0.000 description 1
- 102100028267 Neutral amino acid transporter B(0) Human genes 0.000 description 1
- 108010079246 OMPA outer membrane proteins Proteins 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 208000002389 Pouchitis Diseases 0.000 description 1
- 102000018819 Protein Translocation Systems Human genes 0.000 description 1
- 108700014910 Pseudomonas aeruginosa DNR Proteins 0.000 description 1
- 101100156993 Pseudomonas putida xylC gene Proteins 0.000 description 1
- 101710193192 Putative transcriptional regulator Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- 241001138501 Salmonella enterica Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241001468001 Salmonella virus SP6 Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 101100241087 Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) nrtP gene Proteins 0.000 description 1
- 101100132804 Thermus thermophilus narK1 gene Proteins 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 108010058153 Twin-Arginine-Translocation System Proteins 0.000 description 1
- 108010064721 Type I Secretion Systems Proteins 0.000 description 1
- 108010046504 Type IV Secretion Systems Proteins 0.000 description 1
- 108010050970 Type VII Secretion Systems Proteins 0.000 description 1
- 201000006704 Ulcerative Colitis Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 241000607626 Vibrio cholerae Species 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 241000607734 Yersinia <bacteria> Species 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 238000007171 acid catalysis Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 230000004103 aerobic respiration Effects 0.000 description 1
- 230000009435 amidation Effects 0.000 description 1
- 238000007112 amidation reaction Methods 0.000 description 1
- 150000003862 amino acid derivatives Chemical class 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000009604 anaerobic growth Effects 0.000 description 1
- 101150100261 aniA gene Proteins 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 101150017736 araD gene Proteins 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 235000020299 breve Nutrition 0.000 description 1
- 230000001628 butyrogenic effect Effects 0.000 description 1
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 description 1
- 229910000020 calcium bicarbonate Inorganic materials 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- 229960004203 carnitine Drugs 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- WZNRVWBKYDHTKI-UHFFFAOYSA-N cellulose, acetate 1,2,4-benzenetricarboxylate Chemical compound OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O.OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O.CC(=O)OCC1OC(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(COC(C)=O)O1.CC(=O)OCC1OC(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(COC(C)=O)O1.OC(=O)C1=CC(C(=O)O)=CC=C1C(=O)OCC1C(OC2C(C(OC(=O)C=3C(=CC(=CC=3)C(O)=O)C(O)=O)C(OC(=O)C=3C(=CC(=CC=3)C(O)=O)C(O)=O)C(COC(=O)C=3C(=CC(=CC=3)C(O)=O)C(O)=O)O2)OC(=O)C=2C(=CC(=CC=2)C(O)=O)C(O)=O)C(OC(=O)C=2C(=CC(=CC=2)C(O)=O)C(O)=O)C(OC(=O)C=2C(=CC(=CC=2)C(O)=O)C(O)=O)C(OC(=O)C=2C(=CC(=CC=2)C(O)=O)C(O)=O)O1 WZNRVWBKYDHTKI-UHFFFAOYSA-N 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 108091006116 chimeric peptides Proteins 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 239000000824 cytostatic agent Substances 0.000 description 1
- 230000001085 cytostatic effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000018823 dietary intake Nutrition 0.000 description 1
- 235000020805 dietary restrictions Nutrition 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 206010016165 failure to thrive Diseases 0.000 description 1
- 229910001447 ferric ion Inorganic materials 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 101150077334 focA gene Proteins 0.000 description 1
- 101150003763 gabD1 gene Proteins 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000012246 gene addition Methods 0.000 description 1
- 238000003208 gene overexpression Methods 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 108010064177 glutamine synthetase I Proteins 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 101150077538 gor gene Proteins 0.000 description 1
- 101150107068 gsiB gene Proteins 0.000 description 1
- 239000002271 gyrase inhibitor Substances 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007407 health benefit Effects 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 101150041770 hlyE gene Proteins 0.000 description 1
- 101150083365 hmp gene Proteins 0.000 description 1
- 101150089835 hmpA gene Proteins 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 101150099805 htpG gene Proteins 0.000 description 1
- 150000002432 hydroperoxides Chemical class 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-N hydroperoxyl Chemical compound O[O] OUUQCZGPVNCOIJ-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 229960003444 immunosuppressant agent Drugs 0.000 description 1
- 230000001861 immunosuppressant effect Effects 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000011866 long-term treatment Methods 0.000 description 1
- 235000020905 low-protein-diet Nutrition 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 108091000124 methylmalonyl-CoA epimerase Proteins 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 101150071774 mscL gene Proteins 0.000 description 1
- 101150062306 narK gene Proteins 0.000 description 1
- 101150037810 narT gene Proteins 0.000 description 1
- 101150056725 narX gene Proteins 0.000 description 1
- 101150106215 ndh gene Proteins 0.000 description 1
- 101150044129 nirB gene Proteins 0.000 description 1
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 1
- 230000008599 nitrosative stress Effects 0.000 description 1
- 239000001272 nitrous oxide Substances 0.000 description 1
- 101150021871 norC gene Proteins 0.000 description 1
- 238000003305 oral gavage Methods 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 150000001451 organic peroxides Chemical class 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 101150038927 pdhR gene Proteins 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000010647 peptide synthesis reaction Methods 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 101150107164 phoD gene Proteins 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 101150004519 pilC gene Proteins 0.000 description 1
- 230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 108010010718 poly(3-hydroxyalkanoic acid) synthase Proteins 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 229940068977 polysorbate 20 Drugs 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 235000020004 porter Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011165 process development Methods 0.000 description 1
- 125000001501 propionyl group Chemical group O=C([*])C([H])([H])C([H])([H])[H] 0.000 description 1
- WOMAZEJKVZLLFE-UHFFFAOYSA-N propionylglycine Chemical compound CCC(=O)NCC(O)=O WOMAZEJKVZLLFE-UHFFFAOYSA-N 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000006337 proteolytic cleavage Effects 0.000 description 1
- 101150049824 pstA gene Proteins 0.000 description 1
- 210000004196 psta Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012372 quality testing Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 235000021391 short chain fatty acids Nutrition 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 101150100021 soxR gene Proteins 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 229940032147 starch Drugs 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- VNOYUJKHFWYWIR-FZEDXVDRSA-N succinyl-coa Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCSC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VNOYUJKHFWYWIR-FZEDXVDRSA-N 0.000 description 1
- 101150037939 sufA gene Proteins 0.000 description 1
- 101150102101 sufB gene Proteins 0.000 description 1
- 101150043376 sufC gene Proteins 0.000 description 1
- 101150078941 sufE gene Proteins 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 101150019205 tdcD gene Proteins 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 101150083154 tyrA gene Proteins 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 101150107825 uxuA gene Proteins 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 101150084029 yaeH gene Proteins 0.000 description 1
- 101150062475 yaiA gene Proteins 0.000 description 1
- 101150086536 ybjM gene Proteins 0.000 description 1
- 101150103076 ydcH gene Proteins 0.000 description 1
- 101150035896 ydeN gene Proteins 0.000 description 1
- 101150084511 ygaQ gene Proteins 0.000 description 1
- 101150066411 ytfE gene Proteins 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Obesity (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562199445P | 2015-07-31 | 2015-07-31 | |
| US62/199,445 | 2015-07-31 | ||
| US201662336338P | 2016-05-13 | 2016-05-13 | |
| US62/336,338 | 2016-05-13 | ||
| USPCT/US2016/032565 | 2016-05-13 | ||
| PCT/US2016/032565 WO2016183532A1 (en) | 2015-05-13 | 2016-05-13 | Bacteria engineered to treat a disease or disorder |
| US201662341320P | 2016-05-25 | 2016-05-25 | |
| US62/341,320 | 2016-05-25 | ||
| USPCT/US2016/037098 | 2016-06-10 | ||
| PCT/US2016/037098 WO2016201380A1 (en) | 2015-06-10 | 2016-06-10 | Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid |
| PCT/US2016/044922 WO2017023818A1 (en) | 2015-07-31 | 2016-07-29 | Bacteria engineered to treat disorders involving propionate catabolism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2996535A1 true CA2996535A1 (en) | 2017-02-09 |
Family
ID=57944027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2996535A Abandoned CA2996535A1 (en) | 2015-07-31 | 2016-07-29 | Bacteria engineered to treat disorders involving propionate catabolism |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP3328988A1 (enExample) |
| JP (1) | JP2018521674A (enExample) |
| AU (1) | AU2016303662A1 (enExample) |
| CA (1) | CA2996535A1 (enExample) |
| WO (1) | WO2017023818A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3720458A4 (en) * | 2017-12-05 | 2021-12-08 | BioPlx, Inc. | METHODS AND COMPOSITIONS FOR PREVENTING MICROBIAL INFECTION |
| AU2020288624B2 (en) | 2019-06-04 | 2025-09-25 | Cocoon Biotech Inc. | Silk-based products, formulations, and methods of use |
| CN112143689B (zh) * | 2019-06-28 | 2023-01-03 | 中国科学院微生物研究所 | 重组恶臭假单胞菌株的构建及其在转化苏氨酸合成丙酸中的应用 |
| CN114787344A (zh) | 2019-12-02 | 2022-07-22 | 安斯泰来制药株式会社 | 用于处置癌症的含有ny-eso-1的人工佐剂载体细胞 |
| KR20220130719A (ko) * | 2020-01-17 | 2022-09-27 | 위니베르시트카솔리끄드루뱅 | 엔벨로프 완전성이 변화된 유전자 변형 세균 및 이의 용도 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9107305D0 (en) | 1991-04-08 | 1991-05-22 | Unilever Plc | Probiotic |
| US5989463A (en) | 1997-09-24 | 1999-11-23 | Alkermes Controlled Therapeutics, Inc. | Methods for fabricating polymer-based controlled release devices |
| US6203797B1 (en) | 1998-01-06 | 2001-03-20 | Stephen C. Perry | Dietary supplement and method for use as a probiotic, for alleviating the symptons associated with irritable bowel syndrome |
| EP1135461A4 (en) | 1998-12-02 | 2003-03-26 | Univ Boston | GEN NETWORKS FOR CONTROLLING GENE EXPRESSION |
| EP1034787A1 (en) | 1999-03-11 | 2000-09-13 | Société des Produits Nestlé S.A. | Lactobacillus strains preventing diarrhea caused by pathogenic bacteria |
| US7731976B2 (en) | 2003-08-29 | 2010-06-08 | Cobb And Company, Llp | Treatment of irritable bowel syndrome using probiotic composition |
| CN104024430A (zh) * | 2011-08-26 | 2014-09-03 | 微生物诊断有限责任公司 | 用于诊断和治疗心脏缺陷的方法 |
| US20150246085A1 (en) * | 2012-11-01 | 2015-09-03 | The Sydney Children's Hospital Network (Randwick & Westmead) | Genetically-Modified Probiotic for Treatment of Phenylketonuria |
| WO2014138324A1 (en) | 2013-03-07 | 2014-09-12 | The General Hospital Corporation | Compositions and methods for bacterial delivery of polypeptides |
-
2016
- 2016-07-29 WO PCT/US2016/044922 patent/WO2017023818A1/en not_active Ceased
- 2016-07-29 JP JP2018504991A patent/JP2018521674A/ja active Pending
- 2016-07-29 CA CA2996535A patent/CA2996535A1/en not_active Abandoned
- 2016-07-29 AU AU2016303662A patent/AU2016303662A1/en not_active Abandoned
- 2016-07-29 EP EP16751733.3A patent/EP3328988A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JP2018521674A (ja) | 2018-08-09 |
| WO2017023818A1 (en) | 2017-02-09 |
| AU2016303662A1 (en) | 2018-02-22 |
| EP3328988A1 (en) | 2018-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12409195B2 (en) | Bacteria engineered to reduce hyperphenylalaninemia | |
| US20220233609A1 (en) | Bacteria engineered to treat disorders in which oxalate is detrimental | |
| US20240076645A1 (en) | Bacteria engineered to reduce hyperphenylalaninemia | |
| EP3307870B1 (en) | Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid | |
| US20170253862A1 (en) | Bacteria Engineered to Treat Diseases Associated with Hyperammonemia | |
| US20170216370A1 (en) | Bacteria engineered to treat disorders involving propionate catabolism | |
| WO2017075485A1 (en) | Bacteria engineered to treat disorders in which trimethylamine (tma) is detrimental | |
| CA3167754A1 (en) | Bacteria engineered to treat disorders in which oxalate is detrimental | |
| CA2996535A1 (en) | Bacteria engineered to treat disorders involving propionate catabolism | |
| US20220168362A1 (en) | Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20210721 |
|
| EEER | Examination request |
Effective date: 20210721 |
|
| EEER | Examination request |
Effective date: 20210721 |
|
| EEER | Examination request |
Effective date: 20210721 |
|
| FZDE | Discontinued |
Effective date: 20240115 |