CA2968488C - METHOD FOR DETECTING CONNECTION INTERSECTIONS - Google Patents

METHOD FOR DETECTING CONNECTION INTERSECTIONS

Info

Publication number
CA2968488C
CA2968488C CA2968488A CA2968488A CA2968488C CA 2968488 C CA2968488 C CA 2968488C CA 2968488 A CA2968488 A CA 2968488A CA 2968488 A CA2968488 A CA 2968488A CA 2968488 C CA2968488 C CA 2968488C
Authority
CA
Canada
Prior art keywords
protein
binding agent
library
target
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2968488A
Other languages
English (en)
French (fr)
Other versions
CA2968488A1 (en
Inventor
Csaba Jeney
Original Assignee
Actome GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Actome GmbH filed Critical Actome GmbH
Publication of CA2968488A1 publication Critical patent/CA2968488A1/en
Application granted granted Critical
Publication of CA2968488C publication Critical patent/CA2968488C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2968488A 2014-11-24 2015-11-24 METHOD FOR DETECTING CONNECTION INTERSECTIONS Active CA2968488C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1420852.4 2014-11-24
GBGB1420852.4A GB201420852D0 (en) 2014-11-24 2014-11-24 Method
PCT/GB2015/053573 WO2016083793A1 (en) 2014-11-24 2015-11-24 Method

Publications (2)

Publication Number Publication Date
CA2968488A1 CA2968488A1 (en) 2016-06-02
CA2968488C true CA2968488C (en) 2024-09-10

Family

ID=52292436

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2968488A Active CA2968488C (en) 2014-11-24 2015-11-24 METHOD FOR DETECTING CONNECTION INTERSECTIONS

Country Status (10)

Country Link
US (3) US10605814B2 (OSRAM)
EP (1) EP3224360B1 (OSRAM)
JP (1) JP6871168B2 (OSRAM)
KR (1) KR102422494B1 (OSRAM)
CN (1) CN107430130B (OSRAM)
CA (1) CA2968488C (OSRAM)
ES (1) ES2763563T3 (OSRAM)
GB (1) GB201420852D0 (OSRAM)
SG (2) SG10202008300RA (OSRAM)
WO (1) WO2016083793A1 (OSRAM)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214211A1 (en) * 2016-06-09 2017-12-14 Igc Bio, Inc. Methods for identifying a high affinity antibody
JP7714325B2 (ja) * 2017-01-31 2025-07-29 ４８アワー ディスカバリー インコーポレイテッド 相乗的分子相互作用を決定するための、サイレントに遺伝的にコード化されたナノスケールキャリア上の分子のディスプレイ
WO2019085958A1 (zh) * 2017-11-06 2019-05-09 清华大学 检测生物分子间相互作用及其调控因子的成套试剂与应用
JP7723599B2 (ja) 2018-07-23 2025-08-14 ４８アワー ディスカバリー インコーポレイテッド 遺伝的にコードされた二環式ペプチドライブラリー
CN114041055A (zh) 2019-06-25 2022-02-11 阿克托姆有限责任公司 用于以双组分体系测量分析物的方法和试剂盒及其用途
CN115066434A (zh) 2019-12-03 2022-09-16 阿拉玛生物科学公司 核酸连接的免疫-夹心测定(nulisa)
EP4330678A1 (en) * 2021-04-26 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) High complexity microcompartment-based interaction screening
EP4242656A1 (en) * 2022-03-10 2023-09-13 Actome GmbH Quantitative detection method and corresponding device
EP4547862A1 (en) 2022-06-29 2025-05-07 Actome GmbH Detection of biomolecules in single cells
GB202218453D0 (en) 2022-12-08 2023-01-25 Actome Gmbh Assay system

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
ES2156149T3 (es) 1992-12-04 2001-06-16 Medical Res Council Proteinas de union multivalente y multiespecificas, su fabricacion y su uso.
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
GB0422431D0 (en) * 2004-10-08 2004-11-10 Affitech As Method
EP2209893B1 (en) * 2007-10-12 2013-11-20 Pronota NV Use of aptamers in proteomics
GB0903316D0 (en) * 2009-02-26 2009-04-08 Affitech As Method
RU2562163C2 (ru) * 2009-05-20 2015-09-10 Новиммун С.А. Библиотеки синтетических полипептидов и способы получения вариантов полипептидов с естественным разнообразием
SG177395A1 (en) * 2009-07-07 2012-02-28 Agency Science Tech & Res Methods of identifying a pair of binding partners
CN103119165B (zh) * 2010-09-27 2017-04-12 威泊根私人有限公司 制备富集文库的方法
US9209965B2 (en) 2014-01-14 2015-12-08 Microsemi Semiconductor Ulc Network interface with clock recovery module on line card

Also Published As

Publication number Publication date
US11421347B2 (en) 2022-08-23
GB201420852D0 (en) 2015-01-07
US20230175171A1 (en) 2023-06-08
ES2763563T3 (es) 2020-05-29
JP2017536846A (ja) 2017-12-14
US20210003585A1 (en) 2021-01-07
EP3224360A1 (en) 2017-10-04
WO2016083793A1 (en) 2016-06-02
JP6871168B2 (ja) 2021-05-12
US10605814B2 (en) 2020-03-31
SG11201703689SA (en) 2017-06-29
KR102422494B1 (ko) 2022-07-19
CN107430130A (zh) 2017-12-01
SG10202008300RA (en) 2020-10-29
CA2968488A1 (en) 2016-06-02
US20170269098A1 (en) 2017-09-21
CN107430130B (zh) 2020-07-24
EP3224360B1 (en) 2019-10-09
KR20170086627A (ko) 2017-07-26

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