CA2937088C - Method for generating high-titer hepatitis e virus stocks and titration assay for hepatitis e virus - Google Patents

Method for generating high-titer hepatitis e virus stocks and titration assay for hepatitis e virus Download PDF

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Publication number
CA2937088C
CA2937088C CA2937088A CA2937088A CA2937088C CA 2937088 C CA2937088 C CA 2937088C CA 2937088 A CA2937088 A CA 2937088A CA 2937088 A CA2937088 A CA 2937088A CA 2937088 C CA2937088 C CA 2937088C
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hev
virus
concentration
reaction mixture
titer
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French (fr)
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CA2937088A1 (en
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Brett Buno
Terri Journigan
Joann Hotta
Michael Burdick
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Grifols SA
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Grifols SA
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    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02Recovery or purification
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    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
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    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/706Specific hybridization probes for hepatitis
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/706Specific hybridization probes for hepatitis
    • C12Q1/707Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
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    • C12N2500/00Specific components of cell culture medium
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2770/00011Details
    • C12N2770/28011Hepeviridae
    • C12N2770/28111Hepevirus, e.g. hepatitis E virus
    • C12N2770/28121Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • CCHEMISTRY; METALLURGY
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    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/28011Hepeviridae
    • C12N2770/28111Hepevirus, e.g. hepatitis E virus
    • C12N2770/28151Methods of production or purification of viral material

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  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Oncology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2937088A 2015-07-23 2016-07-22 Method for generating high-titer hepatitis e virus stocks and titration assay for hepatitis e virus Active CA2937088C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562195936P 2015-07-23 2015-07-23
US62/195.936 2015-07-23

Publications (2)

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CA2937088A1 CA2937088A1 (en) 2017-01-23
CA2937088C true CA2937088C (en) 2022-06-21

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US (1) US10208291B2 (enExample)
EP (1) EP3121267B1 (enExample)
JP (1) JP6588397B2 (enExample)
KR (1) KR102154380B1 (enExample)
CN (1) CN106367397B (enExample)
AR (1) AR106015A1 (enExample)
AU (1) AU2016204734B2 (enExample)
BR (1) BR102016016677A2 (enExample)
CA (1) CA2937088C (enExample)
CL (1) CL2016001768A1 (enExample)
ES (1) ES2669021T3 (enExample)
HU (1) HUE036699T2 (enExample)
IL (1) IL246634B (enExample)
MX (1) MX375106B (enExample)
MY (1) MY176204A (enExample)
NZ (1) NZ722213A (enExample)
PL (1) PL3121267T3 (enExample)
PT (1) PT3121267T (enExample)
RU (1) RU2691123C1 (enExample)
SG (1) SG10201605867UA (enExample)
TW (1) TWI666322B (enExample)
UY (1) UY36778A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102158641B1 (ko) * 2019-03-06 2020-09-22 대한민국 야누스 인산화효소 저해제 및 헥사디메트린 브로마이드를 포함하는 바이러스 배양용 조성물 및 이를 이용한 바이러스 배양 방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506604B2 (en) * 1993-06-11 2003-01-14 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US6217858B1 (en) * 1997-02-11 2001-04-17 Hadasit & Medical Research Services & Development Company, Ltd. Pharmaceutical composition for treating hepatitis B virus (HBV) infection
WO2003001198A1 (en) * 2001-06-21 2003-01-03 Regents Of The University Of California Immortalization of human cells by the ectopic expression of human telomerase reverse transcriptase
WO2007046962A2 (en) * 2005-09-02 2007-04-26 The University Of Maryland, Baltimore Cell lines harboring hepatitis virus and methods for using thereof
JP2012504647A (ja) * 2008-10-03 2012-02-23 ザ ロックフェラー ユニヴァーシティ 新規なhcv侵入因子、オクルディン
US9181530B2 (en) * 2011-01-10 2015-11-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Infectious hepatitis E virus genotype 3 recombinants
US9789139B2 (en) * 2011-06-10 2017-10-17 Bluebird Bio, Inc. Gene therapy vectors for adrenoleukodystrophy and adrenomyeloneuropathy

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Publication number Publication date
AR106015A1 (es) 2017-12-06
CN106367397B (zh) 2021-07-20
HUE036699T2 (hu) 2018-07-30
EP3121267A1 (en) 2017-01-25
RU2691123C1 (ru) 2019-06-11
AU2016204734A1 (en) 2017-02-09
EP3121267B1 (en) 2018-03-28
KR20170012140A (ko) 2017-02-02
ES2669021T3 (es) 2018-05-23
JP2017023143A (ja) 2017-02-02
CN106367397A (zh) 2017-02-01
KR102154380B1 (ko) 2020-09-10
PL3121267T3 (pl) 2018-07-31
CL2016001768A1 (es) 2017-05-12
IL246634B (en) 2020-08-31
MX375106B (es) 2025-03-06
BR102016016677A2 (pt) 2019-02-12
TWI666322B (zh) 2019-07-21
IL246634A0 (en) 2016-12-29
UY36778A (es) 2016-09-30
MX2016009048A (es) 2017-01-23
AU2016204734B2 (en) 2019-11-21
SG10201605867UA (en) 2017-02-27
CA2937088A1 (en) 2017-01-23
NZ722213A (en) 2019-04-26
US10208291B2 (en) 2019-02-19
JP6588397B2 (ja) 2019-10-09
US20170022481A1 (en) 2017-01-26
PT3121267T (pt) 2018-04-26
MY176204A (en) 2020-07-24
TW201704468A (zh) 2017-02-01

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