CA2929392A1 - Detecteurs fluorescents codant genetiquement pour la detection de ligandes biaises et signalisation intracelluaire par des voies de camp - Google Patents

Detecteurs fluorescents codant genetiquement pour la detection de ligandes biaises et signalisation intracelluaire par des voies de camp Download PDF

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Publication number
CA2929392A1
CA2929392A1 CA2929392A CA2929392A CA2929392A1 CA 2929392 A1 CA2929392 A1 CA 2929392A1 CA 2929392 A CA2929392 A CA 2929392A CA 2929392 A CA2929392 A CA 2929392A CA 2929392 A1 CA2929392 A1 CA 2929392A1
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CA
Canada
Prior art keywords
seq
protein
camp
amino acid
polypeptide
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2929392A
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English (en)
Inventor
Thomas E. Hughes
Paul H. Tewson
Anne Marie Quinn
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MONTANA MOLECULAR LLC
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MONTANA MOLECULAR LLC
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Application filed by MONTANA MOLECULAR LLC filed Critical MONTANA MOLECULAR LLC
Publication of CA2929392A1 publication Critical patent/CA2929392A1/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Abstract

L'invention concerne de nouveaux détecteurs fluorescents destinés à l'adénosine monophosphate cyclique (cAMP), qui sont basés sur des protéines fluorescentes uniques. Ces détecteurs utilisent moins le spectre visible que les détecteurs à base du transfert FRET, produisent de forts changements de fluorescence, et peuvent être associés les uns aux autres ou bien à d'autres détecteurs, dans un dosage multiplexe sur des lecteurs de plaques fluorescentes standard ou des systèmes d'imagerie de cellules vivantes.
CA2929392A 2013-11-04 2014-11-04 Detecteurs fluorescents codant genetiquement pour la detection de ligandes biaises et signalisation intracelluaire par des voies de camp Pending CA2929392A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361899611P 2013-11-04 2013-11-04
US61/899,611 2013-11-04
PCT/US2014/063916 WO2015066706A2 (fr) 2013-11-04 2014-11-04 Détecteurs fluorescents codant génétiquement pour la détection de ligandes biaisés et signalisation intracelluaire par des voies de camp

Publications (1)

Publication Number Publication Date
CA2929392A1 true CA2929392A1 (fr) 2015-05-07

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2929392A Pending CA2929392A1 (fr) 2013-11-04 2014-11-04 Detecteurs fluorescents codant genetiquement pour la detection de ligandes biaises et signalisation intracelluaire par des voies de camp

Country Status (4)

Country Link
US (2) US11366114B2 (fr)
EP (2) EP3065754B1 (fr)
CA (1) CA2929392A1 (fr)
WO (1) WO2015066706A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013138684A1 (fr) 2012-03-15 2013-09-19 Montana Molecular Llc Capteurs fluorescents génétiquement codés pour détecter la signalisation intracellulaire par l'intermédiaire de voies du diacylglycérol
CA3145920A1 (fr) * 2018-07-30 2020-02-06 Thomas Hughes Biocapteurs pour detecter une signalisation de l'arrestine
CN113024674B (zh) * 2019-12-09 2023-03-28 中国科学院深圳先进技术研究院 荧光亮度宽幅变化的环磷酸腺苷荧光探针
CN113567402B (zh) * 2020-04-29 2023-06-06 中国科学院深圳先进技术研究院 一种cAMP荧光探针G-Flamp1的应用
WO2021217479A1 (fr) * 2020-04-29 2021-11-04 中国科学院深圳先进技术研究院 Application d'une sonde fluorescente d'ampc g-flamp1
CN112480271B (zh) * 2020-12-15 2022-11-01 中国科学院深圳先进技术研究院 高性能红色cAMP荧光探针及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5519163A (en) 1993-10-15 1996-05-21 Merck & Co., Inc. Inhibitors of phosphoinositide-specific phospholipase C
US8669074B2 (en) 1996-01-31 2014-03-11 The Regents Of The University Of California Chimeric phosphorylation indicator
DK0986753T4 (da) 1997-04-07 2005-12-19 Bioimage As Fremgangsmåde til uddragning af kvantitativ information, hvad angår en påvirkning af et cellulært respons
US7338784B2 (en) 1998-12-11 2008-03-04 Clontech Laboratories, Inc Nucleic acids encoding chromophores/fluorophores and methods for using the same
US6469154B1 (en) 1999-05-21 2002-10-22 The Regents Of The University Of California Fluorescent protein indicators
US7060793B2 (en) 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US8236523B2 (en) 2004-08-23 2012-08-07 The Johns Hopkins University Camp reporters and high throughput assays
WO2006054167A2 (fr) 2004-11-19 2006-05-26 Novasite Pharmaceuticals, Inc. Biodetecteur permettant de detecter des taux de camp et procedes d'utilisation associes
DK1994149T3 (da) 2006-01-25 2011-01-17 Evrogen Ip Nye fluorescerende proteiner og fremgangsmåder til anvendelse deraf
JP6110591B2 (ja) 2008-05-19 2017-04-05 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ ｃＡＭＰのためのルシフェラーゼバイオセンサー
EP2496697B1 (fr) 2009-11-06 2017-04-12 Howard Hughes Medical Institute Indicateurs du calcium génétiquement codés et leurs procédés d'utilisation
CA2801781C (fr) 2010-07-21 2018-02-27 Rigel Pharmaceuticals, Inc. Inhibiteurs de proteine kinase c et leurs utilisations
WO2013138684A1 (fr) 2012-03-15 2013-09-19 Montana Molecular Llc Capteurs fluorescents génétiquement codés pour détecter la signalisation intracellulaire par l'intermédiaire de voies du diacylglycérol
US20170247769A1 (en) * 2016-02-22 2017-08-31 Carnegie Institution Of Washington Biosensors and methods of use

Also Published As

Publication number Publication date
EP3065754B1 (fr) 2020-06-10
US20160274109A1 (en) 2016-09-22
WO2015066706A2 (fr) 2015-05-07
US20220404357A1 (en) 2022-12-22
US11366114B2 (en) 2022-06-21
WO2015066706A3 (fr) 2015-06-25
EP3738971A1 (fr) 2020-11-18
EP3065754A4 (fr) 2017-07-26
EP3065754A2 (fr) 2016-09-14

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