CA2907491C - Procede d'extraction d'une enzyme a partir d'un tissu vegetal ou animal - Google Patents

Procede d'extraction d'une enzyme a partir d'un tissu vegetal ou animal Download PDF

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Publication number
CA2907491C
CA2907491C CA2907491A CA2907491A CA2907491C CA 2907491 C CA2907491 C CA 2907491C CA 2907491 A CA2907491 A CA 2907491A CA 2907491 A CA2907491 A CA 2907491A CA 2907491 C CA2907491 C CA 2907491C
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Canada
Prior art keywords
enzyme
amy797e
polypeptide
sample
detection
Prior art date
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CA2907491A
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English (en)
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CA2907491A1 (fr
Inventor
Mahathelge Dilip DIAS
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Syngenta Participations AG
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Syngenta Participations AG
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Publication date
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Publication of CA2907491A1 publication Critical patent/CA2907491A1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

L'invention concerne un procédé d'extraction et de détection d'un peptide dans des tissus végétaux transgéniques, un composé favorisant la solubilité non immunogène étant utilisé pour libérer l'enzyme dans la fraction de la solution pendant le processus de purification. Dans certains modes de réalisation, l'invention concerne un procédé d'extraction et de détection de l'enzyme Amy797E, qui est une alpha-amylase hétérologue thermo-tolérante, dans des tissus du maïs 3272 en utilisant une amylase non immunogène pendant le processus de purification. L'invention permet un seuil de détection de 1:1000 de l'amylase Amy797E par le test ELISA (méthode immuno-enzymatique).
CA2907491A 2013-02-15 2013-02-15 Procede d'extraction d'une enzyme a partir d'un tissu vegetal ou animal Active CA2907491C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2013/026303 WO2014126579A1 (fr) 2013-02-15 2013-02-15 Procédé d'extraction d'une enzyme à partir d'un tissu végétal ou animal

Publications (2)

Publication Number Publication Date
CA2907491A1 CA2907491A1 (fr) 2014-08-21
CA2907491C true CA2907491C (fr) 2018-02-20

Family

ID=51354446

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2907491A Active CA2907491C (fr) 2013-02-15 2013-02-15 Procede d'extraction d'une enzyme a partir d'un tissu vegetal ou animal

Country Status (2)

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CA (1) CA2907491C (fr)
WO (1) WO2014126579A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109022540A (zh) * 2018-06-21 2018-12-18 北京市农林科学院 快速检测转基因产品的试纸条
CN108828230B (zh) * 2018-06-21 2021-04-30 北京市农林科学院 核酸层析快速检测转基因产品的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5399484A (en) * 1991-10-08 1995-03-21 Eastman Kodak Company Use of blocking protein with high pH extraction in method to determine a microorganism associated with periodontal disease and kit useful therefor
CA2537105A1 (fr) * 2003-08-27 2005-03-10 Orf Liftaekni Hf. Processus de clivage proteolytique et purification de proteines de recombinaison
EP1868426B1 (fr) * 2005-03-16 2018-02-21 Syngenta Participations AG Mais 3272 et procedes pour le detecter

Also Published As

Publication number Publication date
WO2014126579A1 (fr) 2014-08-21
CA2907491A1 (fr) 2014-08-21

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Effective date: 20160104