CA2896197C - Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays - Google Patents

Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays Download PDF

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Publication number
CA2896197C
CA2896197C CA2896197A CA2896197A CA2896197C CA 2896197 C CA2896197 C CA 2896197C CA 2896197 A CA2896197 A CA 2896197A CA 2896197 A CA2896197 A CA 2896197A CA 2896197 C CA2896197 C CA 2896197C
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Prior art keywords
spectral
image data
spectrum
light
image
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English (en)
French (fr)
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CA2896197A1 (en
Inventor
Karl Garsha
Michael Otter
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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Priority to CA3053060A priority Critical patent/CA3053060C/en
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0297—Constructional arrangements for removing other types of optical noise or for performing calibration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/80—Analysis of captured images to determine intrinsic or extrinsic camera parameters, i.e. camera calibration
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G01J2003/2826—Multispectral imaging, e.g. filter imaging

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Mathematical Physics (AREA)
  • Biochemistry (AREA)
  • Optics & Photonics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Processing (AREA)
CA2896197A 2013-01-31 2014-01-31 Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays Active CA2896197C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3053060A CA3053060C (en) 2013-01-31 2014-01-31 Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361759262P 2013-01-31 2013-01-31
US61/759,262 2013-01-31
PCT/EP2014/051920 WO2014118326A2 (en) 2013-01-31 2014-01-31 Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays

Related Child Applications (1)

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CA3053060A Division CA3053060C (en) 2013-01-31 2014-01-31 Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays

Publications (2)

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CA2896197A1 CA2896197A1 (en) 2014-08-07
CA2896197C true CA2896197C (en) 2019-10-15

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CA3053060A Active CA3053060C (en) 2013-01-31 2014-01-31 Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays

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US (3) US10012537B2 (enExample)
EP (1) EP2951543B1 (enExample)
JP (4) JP6392785B2 (enExample)
AU (2) AU2014211384A1 (enExample)
CA (2) CA2896197C (enExample)
WO (1) WO2014118326A2 (enExample)

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Publication number Publication date
US10012537B2 (en) 2018-07-03
JP7200204B2 (ja) 2023-01-06
JP6392785B2 (ja) 2018-09-19
CA3053060A1 (en) 2014-08-07
AU2018201146A1 (en) 2018-03-08
CA3053060C (en) 2022-04-19
US20150369664A1 (en) 2015-12-24
US20180292260A1 (en) 2018-10-11
JP2016510408A (ja) 2016-04-07
US20200209061A1 (en) 2020-07-02
CA2896197A1 (en) 2014-08-07
US11566941B2 (en) 2023-01-31
AU2014211384A1 (en) 2015-07-02
EP2951543A2 (en) 2015-12-09
US10598548B2 (en) 2020-03-24
WO2014118326A9 (en) 2014-10-16
WO2014118326A3 (en) 2014-12-04
JP2019124695A (ja) 2019-07-25
JP2018119968A (ja) 2018-08-02
EP2951543B1 (en) 2022-05-25
JP2021047207A (ja) 2021-03-25
WO2014118326A2 (en) 2014-08-07

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