CA2877368C - Kit for isothermal dna amplification starting from an rna template - Google Patents
Kit for isothermal dna amplification starting from an rna template Download PDFInfo
- Publication number
- CA2877368C CA2877368C CA2877368A CA2877368A CA2877368C CA 2877368 C CA2877368 C CA 2877368C CA 2877368 A CA2877368 A CA 2877368A CA 2877368 A CA2877368 A CA 2877368A CA 2877368 C CA2877368 C CA 2877368C
- Authority
- CA
- Canada
- Prior art keywords
- dna
- rna
- template
- primer
- inosine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/538,955 US9777319B2 (en) | 2012-06-29 | 2012-06-29 | Method for isothermal DNA amplification starting from an RNA template |
US13/538,962 US20140004509A1 (en) | 2012-06-29 | 2012-06-29 | Kit for isothermal dna amplification starting from an rna template |
US13/538,955 | 2012-06-29 | ||
US13/538,962 | 2012-06-29 | ||
PCT/US2013/048229 WO2014004852A2 (en) | 2012-06-29 | 2013-06-27 | Kit for isothermal dna amplification starting from an rna template |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2877368A1 CA2877368A1 (en) | 2014-01-03 |
CA2877368C true CA2877368C (en) | 2021-07-13 |
Family
ID=49784019
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2877368A Active CA2877368C (en) | 2012-06-29 | 2013-06-27 | Kit for isothermal dna amplification starting from an rna template |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP2867366B1 (ja) |
JP (1) | JP6382189B2 (ja) |
CN (1) | CN104583413B (ja) |
CA (1) | CA2877368C (ja) |
WO (1) | WO2014004852A2 (ja) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130323795A1 (en) | 2007-01-10 | 2013-12-05 | General Electric Company | Endonuclase-assisted isothermal amplification using contamination-free reagents |
UA125112C2 (uk) | 2012-04-09 | 2022-01-12 | Енвіролоджикс Інк. | Спосіб ампліфікації полінуклеотиду |
JP6786390B2 (ja) * | 2013-08-13 | 2020-11-25 | ゼネラル・エレクトリック・カンパニイ | コンタミネーションフリー試薬を使用したエンドヌクレアーゼ支援等温増幅 |
US11505836B2 (en) | 2014-04-22 | 2022-11-22 | Envirologix Inc. | Compositions and methods for enhancing and/or predicting DNA amplification |
AU2015336086C1 (en) * | 2014-10-20 | 2021-07-29 | Envirologix Inc. | Compositions and methods for detecting an RNA virus |
AU2017265723B2 (en) * | 2016-05-17 | 2023-04-13 | Moderna Enzymatics Co. Ltd. | Method of amplifying circular dna |
CN107400701A (zh) * | 2016-05-18 | 2017-11-28 | 上海德沛生物科技有限公司 | 环状rna分子的扩增和测序方法 |
CN107541507A (zh) * | 2016-06-29 | 2018-01-05 | 厦门大学 | 一种rna反转录扩增方法 |
CA3029682A1 (en) * | 2016-06-30 | 2018-01-04 | Click Diagnostics, Inc. | Devices and methods for nucleic acid extraction |
US10350307B2 (en) * | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
JP7029138B2 (ja) * | 2018-03-05 | 2022-03-03 | 国立大学法人埼玉大学 | 連結核酸断片の製造方法、連結核酸断片、及び連結核酸断片から構成されるライブラリ |
CN110819673B (zh) * | 2018-08-07 | 2021-07-13 | 华中农业大学 | 一种原核生物cDNA5′末端快速扩增方法 |
WO2020132714A1 (en) * | 2018-12-24 | 2020-07-02 | Deakin University | Method for amplification of nucleic acids |
CN116004681B (zh) * | 2022-08-15 | 2023-08-29 | 南京诺唯赞生物科技股份有限公司 | 一种提高topo克隆中载体连接效率的方法及试剂盒 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6977153B2 (en) * | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
EP1851331B1 (fr) * | 2005-02-25 | 2016-02-24 | Commissariat à l'Énergie Atomique et aux Énergies Alternatives | Procede et dispositif pour separer des cibles moleculaires dans un melange complexe |
CN101203618B (zh) * | 2005-05-26 | 2013-03-13 | 人类遗传标记控股有限公司 | 使用含有非常规碱基的引物的等温链置换扩增 |
JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
US8202972B2 (en) * | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
US20120021461A1 (en) * | 2009-01-21 | 2012-01-26 | Human Genetic Signatures Pty. Ltd. | Isothermal strand displacement amplification |
JP2012080871A (ja) * | 2009-12-14 | 2012-04-26 | National Agriculture & Food Research Organization | Rnaの直接検出法 |
US20140255928A1 (en) * | 2013-03-11 | 2014-09-11 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
-
2013
- 2013-06-27 CA CA2877368A patent/CA2877368C/en active Active
- 2013-06-27 WO PCT/US2013/048229 patent/WO2014004852A2/en active Application Filing
- 2013-06-27 CN CN201380044858.1A patent/CN104583413B/zh active Active
- 2013-06-27 JP JP2015520511A patent/JP6382189B2/ja active Active
- 2013-06-27 EP EP13809883.5A patent/EP2867366B1/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2014004852A2 (en) | 2014-01-03 |
CN104583413B (zh) | 2018-10-19 |
EP2867366B1 (en) | 2018-05-16 |
CA2877368A1 (en) | 2014-01-03 |
EP2867366A4 (en) | 2016-04-20 |
WO2014004852A3 (en) | 2014-04-24 |
JP2015526069A (ja) | 2015-09-10 |
CN104583413A (zh) | 2015-04-29 |
EP2867366A2 (en) | 2015-05-06 |
JP6382189B2 (ja) | 2018-08-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20180626 |