CA2872305A1 - Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands - Google Patents

Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands Download PDF

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Publication number
CA2872305A1
CA2872305A1 CA 2872305 CA2872305A CA2872305A1 CA 2872305 A1 CA2872305 A1 CA 2872305A1 CA 2872305 CA2872305 CA 2872305 CA 2872305 A CA2872305 A CA 2872305A CA 2872305 A1 CA2872305 A1 CA 2872305A1
Authority
CA
Canada
Prior art keywords
fusion protein
moiety
organic target
protein
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2872305
Other languages
English (en)
French (fr)
Inventor
My Hedhammar
Jan Johansson
Anna Rising
Per-Ake Nygren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunovia AB
Spiber Technologies AB
Original Assignee
Immunovia AB
Spiber Technologies AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunovia AB, Spiber Technologies AB filed Critical Immunovia AB
Publication of CA2872305A1 publication Critical patent/CA2872305A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CA 2872305 2012-05-02 2013-05-02 Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands Abandoned CA2872305A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12166392 2012-05-02
EP12166392.6 2012-05-02
PCT/EP2013/059145 WO2013164404A1 (en) 2012-05-02 2013-05-02 Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands

Publications (1)

Publication Number Publication Date
CA2872305A1 true CA2872305A1 (en) 2013-11-07

Family

ID=48468238

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2872305 Abandoned CA2872305A1 (en) 2012-05-02 2013-05-02 Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands

Country Status (6)

Country Link
US (1) US9856308B2 (enExample)
EP (1) EP2844361B1 (enExample)
JP (1) JP6302895B2 (enExample)
CN (1) CN104470607B (enExample)
CA (1) CA2872305A1 (enExample)
WO (1) WO2013164404A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4159833A3 (en) 2009-12-09 2023-07-26 The Procter & Gamble Company Fabric and home care products
CN104877031B (zh) * 2015-05-23 2019-04-23 浙江省农业科学院 一种新型融合蛋白nscr5及其制备方法
WO2017020291A1 (en) 2015-08-06 2017-02-09 Wuxi Biologics (Shanghai) Co. Ltd. Novel anti-pd-l1 antibodies
WO2017188346A1 (ja) * 2016-04-27 2017-11-02 国立大学法人 東京大学 マイクロファイバーを用いた血中循環細胞の捕捉及び回収用材料及び当該材料を用いる方法
EP3263593A1 (en) * 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
WO2020158947A1 (ja) * 2019-01-31 2020-08-06 Ｓｐｉｂｅｒ株式会社 ポリペプチド
CN114980925A (zh) * 2019-11-18 2022-08-30 善萃科思生物科技公司 包含抗CD300c单克隆抗体的用于预防或治疗癌症的组合物
CN117756926B (zh) * 2023-12-26 2024-06-07 广东普言生物科技有限公司 一种重组ⅩⅦ型胶原蛋白Pro.C17及其制备方法和应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2712304C (en) 1994-06-10 2011-10-25 United States Surgical Corporation Recombinant chimeric proteins and methods of use thereof
JP2000125872A (ja) 1998-09-07 2000-05-09 Terumo Corp 三量体キメラタンパク質およびキメラタンパク質を含有するコラーゲンマトリックス
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
SE0200943D0 (sv) * 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
US20050261479A1 (en) * 2004-04-29 2005-11-24 Christian Hoffmann Method for purifying and recovering silk proteins using magnetic affinity separation
WO2006076711A2 (en) 2005-01-14 2006-07-20 Trustees Of Tufts College Fibrous protein fusions and use thereof in the formation of advanced organic/inorganic composite materials
AU2006333611B2 (en) 2005-12-30 2012-04-26 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins
EP1852470A1 (en) 2006-05-03 2007-11-07 Technische Universität München Multilayer Silk Protein Films
US8431158B2 (en) * 2007-11-26 2013-04-30 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Compositions comprising fibrous polypeptides and polysaccharides
CA2753287A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
DK3178843T3 (da) * 2010-10-27 2019-08-19 Spiber Tech Ab Edderkoppesilke-fusionsproteinstrukturer til at binde til et organisk mål

Also Published As

Publication number Publication date
EP2844361B1 (en) 2020-03-25
JP2015518489A (ja) 2015-07-02
WO2013164404A1 (en) 2013-11-07
US20150119554A1 (en) 2015-04-30
CN104470607A (zh) 2015-03-25
CN104470607B (zh) 2017-04-05
JP6302895B2 (ja) 2018-03-28
US9856308B2 (en) 2018-01-02
EP2844361A1 (en) 2015-03-11

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180430

FZDE Discontinued

Effective date: 20210115