CA2866587C - Analysis of measurements of a polymer - Google Patents
Analysis of measurements of a polymer Download PDFInfo
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- CA2866587C CA2866587C CA2866587A CA2866587A CA2866587C CA 2866587 C CA2866587 C CA 2866587C CA 2866587 A CA2866587 A CA 2866587A CA 2866587 A CA2866587 A CA 2866587A CA 2866587 C CA2866587 C CA 2866587C
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| US8285719B1 (en) | 2008-08-08 | 2012-10-09 | The Research Foundation Of State University Of New York | System and method for probabilistic relational clustering |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
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| US10689697B2 (en) | 2014-10-16 | 2020-06-23 | Oxford Nanopore Technologies Ltd. | Analysis of a polymer |
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| US10302972B2 (en) | 2015-01-23 | 2019-05-28 | Pacific Biosciences Of California, Inc. | Waveguide transmission |
| EP3254103B1 (en) | 2015-02-05 | 2023-08-16 | President and Fellows of Harvard College | Nanopore sensor including fluidic passage |
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| WO2017024049A1 (en) | 2015-08-06 | 2017-02-09 | Pacific Biosciences Of California, Inc. | Single-molecule nanofet sequencing systems and methods |
| US10783268B2 (en) | 2015-11-10 | 2020-09-22 | Hewlett Packard Enterprise Development Lp | Data allocation based on secure information retrieval |
| CN116496364A (zh) | 2016-03-02 | 2023-07-28 | 牛津纳米孔科技公开有限公司 | 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔 |
| US11514289B1 (en) | 2016-03-09 | 2022-11-29 | Freenome Holdings, Inc. | Generating machine learning models using genetic data |
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| MX2019000037A (es) * | 2016-07-06 | 2019-07-10 | Guardant Health Inc | Metodos para perfilado de fragmentoma de acidos nucleicos libres de celula. |
| WO2018034745A1 (en) * | 2016-08-18 | 2018-02-22 | The Regents Of The University Of California | Nanopore sequencing base calling |
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| EP3682020B1 (en) * | 2017-09-15 | 2025-09-03 | Illumina, Inc. | Sequence-detection system |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| GB2569630B (en) | 2017-12-21 | 2022-10-12 | Sharp Life Science Eu Ltd | Droplet Interfaces in Electro-wetting Devices |
| SG11201903333SA (en) | 2017-12-29 | 2019-08-27 | Clear Labs Inc | Automated priming and library loading services |
| WO2019149626A1 (de) | 2018-02-02 | 2019-08-08 | Bayer Aktiengesellschaft | Bekämpfung resistenter schadorganismen |
| WO2019204720A1 (en) * | 2018-04-20 | 2019-10-24 | The Regents Of The University Of California | Nucleic acid sequencing methods and computer-readable media for practicing same |
| EP3801901A1 (en) | 2018-05-24 | 2021-04-14 | Oxford Nanopore Technologies Limited | Droplet interfaces in electro-wetting devices |
| GB201814369D0 (en) | 2018-09-04 | 2018-10-17 | Oxford Nanopore Tech Ltd | Method for determining a polymersequence |
| GB201819378D0 (en) * | 2018-11-28 | 2019-01-09 | Oxford Nanopore Tech Ltd | Analysis of nanopore signal using a machine-learning technique |
| EP3887046A1 (en) | 2018-11-28 | 2021-10-06 | Oxford Nanopore Technologies Limited | Sensing system and method of operation |
| EP3938779B1 (en) | 2019-03-12 | 2025-07-30 | Oxford Nanopore Technologies plc | Nanopore sensing device and method |
| US20220254446A1 (en) * | 2019-05-22 | 2022-08-11 | Icahn School Of Medicine At Mount Sinai | Method for de novo detection, identification and fine mapping of multiple forms of nucleic acid modifications |
| CN110628597B (zh) * | 2019-10-19 | 2023-04-25 | 上海新微技术研发中心有限公司 | 拉曼光谱法生物分子测序方法 |
| US11651281B2 (en) * | 2020-05-18 | 2023-05-16 | International Business Machines Corporation | Feature catalog enhancement through automated feature correlation |
| CN115989410A (zh) | 2020-07-17 | 2023-04-18 | 牛津纳米孔科技公开有限公司 | 纳米孔感测装置 |
| CN113030548A (zh) * | 2021-03-09 | 2021-06-25 | 南京邮电大学 | 基于局域阈值的纳米孔检测电流信号特征信息提取方法及装置 |
| GB202103605D0 (en) | 2021-03-16 | 2021-04-28 | Oxford Nanopore Tech Ltd | Alignment of target and reference sequences of polymer units |
| CN117337333A (zh) | 2021-05-19 | 2024-01-02 | 牛津纳米孔科技公开有限公司 | 用于补体链测序的方法 |
| WO2023029044A1 (zh) * | 2021-09-06 | 2023-03-09 | 百图生科(北京)智能技术有限公司 | 用于单细胞测序的方法、装置、设备、介质和程序产品 |
| CN113837036B (zh) * | 2021-09-09 | 2024-08-02 | 成都齐碳科技有限公司 | 生物聚合物的表征方法、装置、设备及计算机存储介质 |
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| GB202215442D0 (en) | 2022-10-19 | 2022-11-30 | Oxford Nanopore Tech Plc | Analysis of a polymer |
| EP4616402A1 (en) | 2022-11-10 | 2025-09-17 | Bayer Aktiengesellschaft | Targeted crop protection product application based on genetic profiles |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| JP7630538B2 (ja) * | 2023-03-15 | 2025-02-17 | Dic株式会社 | 樹脂物性値予測装置及び樹脂物性値の予測方法 |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
Family Cites Families (78)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6128587A (en) * | 1997-01-14 | 2000-10-03 | The Regents Of The University Of California | Method and apparatus using Bayesian subfamily identification for sequence analysis |
| JPH11178575A (ja) * | 1997-12-22 | 1999-07-06 | Hitachi Ltd | Ｄｎａ塩基配列解析装置、方法及び記録媒体 |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| WO2000039333A1 (en) | 1998-12-23 | 2000-07-06 | Jones Elizabeth Louise | Sequencing method using magnifying tags |
| WO2000078668A1 (en) | 1999-06-22 | 2000-12-28 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| AU2002239284A1 (en) | 2000-11-27 | 2002-06-03 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US20020197618A1 (en) | 2001-01-20 | 2002-12-26 | Sampson Jeffrey R. | Synthesis and amplification of unstructured nucleic acids for rapid sequencing |
| JP2002325581A (ja) * | 2001-04-27 | 2002-11-12 | Adgene Co Ltd | 核酸溶解曲線及び核酸解離曲線を用いた未知あるいは既知核酸変異検出法及び表示法 |
| JP2003256435A (ja) | 2002-03-06 | 2003-09-12 | Fujitsu Ltd | 配列データ統合処理方法、配列データ統合処理装置及び配列データ統合処理プログラム |
| US7220405B2 (en) | 2003-09-08 | 2007-05-22 | E. I. Du Pont De Nemours And Company | Peptide-based conditioners and colorants for hair, skin, and nails |
| JP2005176730A (ja) | 2003-12-19 | 2005-07-07 | Hitachi Ltd | ｃＤＮＡ配列をゲノム配列にマッピングする方法 |
| US20050136408A1 (en) | 2003-12-19 | 2005-06-23 | May Tom-Moy | Methods and systems for characterizing a polymer |
| US7279337B2 (en) | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| KR100614827B1 (ko) | 2004-05-06 | 2006-08-25 | 재단법인서울대학교산학협력재단 | 양방향 은닉 마코프 모델을 이용한 완숙한마이크로알엔에이 위치예측방법 및 이를 구현하기 위한컴퓨터 프로그램을 기록한 저장매체 |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| US20060019259A1 (en) | 2004-07-22 | 2006-01-26 | Joyce Timothy H | Characterization of biopolymers by resonance tunneling and fluorescence quenching |
| US20130071837A1 (en) | 2004-10-06 | 2013-03-21 | Stephen N. Winters-Hilt | Method and System for Characterizing or Identifying Molecules and Molecular Mixtures |
| US20060086626A1 (en) | 2004-10-22 | 2006-04-27 | Joyce Timothy H | Nanostructure resonant tunneling with a gate voltage source |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| WO2007065025A2 (en) | 2005-11-29 | 2007-06-07 | Wisconsin Alumni Research Foundation | Method of dna analysis using micro/nanochannel |
| WO2007117832A2 (en) | 2006-03-12 | 2007-10-18 | Applera Corporation | Methods of detecting target nucleic acids |
| US8116988B2 (en) | 2006-05-19 | 2012-02-14 | The University Of Chicago | Method for indexing nucleic acid sequences for computer based searching |
| US7731826B2 (en) | 2006-08-17 | 2010-06-08 | Electronic Bio Sciences, Llc | Controlled translocation of a polymer in an electrolytic sensing system |
| US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
| DK2122344T3 (da) | 2007-02-20 | 2019-07-15 | Oxford Nanopore Tech Ltd | Lipiddobbeltlags-sensorsystem |
| CN101680873B (zh) * | 2007-04-04 | 2015-11-25 | 加利福尼亚大学董事会 | 使用纳米孔的组合物、设备、系统和方法 |
| EP3540436B1 (en) | 2007-09-12 | 2023-11-01 | President And Fellows Of Harvard College | High-resolution molecular sensor |
| GB2453377A (en) * | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8628940B2 (en) | 2008-09-24 | 2014-01-14 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| EP2682460B1 (en) | 2008-07-07 | 2017-04-26 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| EP2310534B1 (en) * | 2008-07-07 | 2018-09-05 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| HUE029215T2 (en) | 2008-09-22 | 2017-02-28 | Univ Washington | MSP nanopores and related procedures |
| CN102257162A (zh) | 2008-10-29 | 2011-11-23 | 波士顿大学理事会 | 保持序列的dna转化 |
| US8452546B1 (en) | 2008-11-07 | 2013-05-28 | Electronic Biosciences, Inc. | Method for deducing a polymer sequence from a nominal base-by-base measurement |
| GB0820927D0 (en) * | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| EP2391639A1 (en) | 2009-01-30 | 2011-12-07 | Oxford Nanopore Technologies Limited | Enzyme mutant |
| KR101797773B1 (ko) | 2009-01-30 | 2017-11-15 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 막횡단 시퀀싱에서 핵산 구축물용 어댑터 |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| WO2010117470A2 (en) | 2009-04-10 | 2010-10-14 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| EP2422198B1 (en) | 2009-04-20 | 2013-09-25 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor array |
| AU2010307229B2 (en) | 2009-09-18 | 2016-02-25 | President And Fellows Of Harvard College | Bare single-layer graphene membrane having a nanopore enabling high-sensitivity molecular detection and analysis |
| US9127313B2 (en) | 2009-12-01 | 2015-09-08 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
| US8748091B2 (en) | 2009-12-18 | 2014-06-10 | The Board Of Trustees Of The University Of Illinois | Characterizing stretched polynucleotides in a synthetic nanopassage |
| US8324914B2 (en) | 2010-02-08 | 2012-12-04 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
| CA3116307C (en) | 2010-02-23 | 2023-10-17 | University Of Washington | Artificial mycolic acid membranes |
| WO2012005857A1 (en) | 2010-06-08 | 2012-01-12 | President And Fellows Of Harvard College | Nanopore device with graphene supported artificial lipid membrane |
| US20140051068A1 (en) | 2010-09-07 | 2014-02-20 | The Regents Of The University Of California | Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme |
| WO2012109483A2 (en) | 2011-02-09 | 2012-08-16 | Life Technologies Corporation | A method of analysis of genetic markers |
| EP2673638B1 (en) | 2011-02-11 | 2019-10-30 | Oxford Nanopore Technologies Limited | Mutant pores |
| US20120316075A1 (en) | 2011-03-30 | 2012-12-13 | Noblegen Biosciences, Inc. | Sequence preserved dna conversion for optical nanopore sequencing |
| US9702849B2 (en) | 2011-04-04 | 2017-07-11 | President And Fellows Of Harvard College | Nanopore sensing by local electrical potential measurement |
| WO2012164270A1 (en) | 2011-05-27 | 2012-12-06 | Oxford Nanopore Technologies Limited | Coupling method |
| US9145623B2 (en) | 2011-07-20 | 2015-09-29 | Thermo Fisher Scientific Oy | Transposon nucleic acids comprising a calibration sequence for DNA sequencing |
| IN2014DN00221A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2011-07-25 | 2015-06-05 | Oxford Nanopore Tech Ltd | |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| JP6226869B2 (ja) | 2011-10-21 | 2017-11-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素法 |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| CN107828877A (zh) | 2012-01-20 | 2018-03-23 | 吉尼亚科技公司 | 基于纳米孔的分子检测与测序 |
| IN2014DN06795A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2012-02-16 | 2015-05-22 | Oxford Nanopore Tech Ltd | |
| WO2013123379A2 (en) | 2012-02-16 | 2013-08-22 | The Regents Of The University Of California | Nanopore sensor for enzyme-mediated protein translocation |
| EP3192804B1 (en) | 2012-04-10 | 2019-08-28 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| US10612083B2 (en) | 2012-04-19 | 2020-04-07 | University Of Washington Through Its Center For Commercialization | Methods and compositions for generating reference maps for nanopore-based polymer analysis |
| US9116118B2 (en) | 2012-06-08 | 2015-08-25 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| US10808231B2 (en) | 2012-07-19 | 2020-10-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| CN104936682B (zh) | 2012-10-26 | 2017-12-15 | 牛津纳米孔技术公司 | 微滴界面 |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| JP6800749B2 (ja) | 2013-11-26 | 2020-12-23 | イルミナ インコーポレイテッド | ポリヌクレオチド配列決定のための組成物及び方法 |
| WO2015140535A1 (en) | 2014-03-21 | 2015-09-24 | Oxford Nanopore Technologies Limited | Analysis of a polymer from multi-dimensional measurements |
| GB201408652D0 (en) | 2014-05-15 | 2014-07-02 | Oxford Nanopore Tech Ltd | Model adjustment during analysis of a polymer from nanopore measurements |
| US9925679B2 (en) | 2014-05-19 | 2018-03-27 | I+D+M Creative, Llc | Devices and methods for assisting with slicing items |
| US10689697B2 (en) | 2014-10-16 | 2020-06-23 | Oxford Nanopore Technologies Ltd. | Analysis of a polymer |
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