CA2856345C - Dispositif a convection thermique pour une reaction en chaine de la polymerase - Google Patents
Dispositif a convection thermique pour une reaction en chaine de la polymerase Download PDFInfo
- Publication number
- CA2856345C CA2856345C CA2856345A CA2856345A CA2856345C CA 2856345 C CA2856345 C CA 2856345C CA 2856345 A CA2856345 A CA 2856345A CA 2856345 A CA2856345 A CA 2856345A CA 2856345 C CA2856345 C CA 2856345C
- Authority
- CA
- Canada
- Prior art keywords
- test tube
- diameter section
- passage
- heat dissipation
- dissipation base
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000003752 polymerase chain reaction Methods 0.000 title claims abstract description 36
- 238000012360 testing method Methods 0.000 claims abstract description 38
- 238000003780 insertion Methods 0.000 claims abstract description 8
- 230000037431 insertion Effects 0.000 claims abstract description 8
- 230000017525 heat dissipation Effects 0.000 claims description 17
- 230000003247 decreasing effect Effects 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 18
- 238000000137 annealing Methods 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 150000007523 nucleic acids Chemical group 0.000 description 2
- 239000003570 air Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
- B01L2400/0445—Natural or forced convection
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
- B01L9/065—Test-tube stands; Test-tube holders specially adapted for capillary tubes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La présente invention concerne un dispositif à convection thermique pour une réaction en chaîne de la polymérase (PCR). Le dispositif est utilisé pour supporter un tube à essai de sorte qu'une PCR est réalisée dans le tube à essai. Le dispositif comprend une base de rayonnement de chaleur qui a un corps et un passage présent à travers le corps destiné à être utilisé pour l'insertion du tube à essai, le passage ayant une grande section de diamètre et une petite section de diamètre positionnée au-dessous de la grande section de diamètre. Par conséquent, il peut être assuré qu'au cours du procédé de PCR, la température de surface du liquide mélangé est inférieure à la température nécessaire dans une réaction d'hybridation d'amorce.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2011/001941 WO2013075263A1 (fr) | 2011-11-22 | 2011-11-22 | Dispositif à convection thermique pour une réaction en chaîne de la polymérase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2856345A1 CA2856345A1 (fr) | 2013-05-30 |
| CA2856345C true CA2856345C (fr) | 2017-10-24 |
Family
ID=48468973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2856345A Active CA2856345C (fr) | 2011-11-22 | 2011-11-22 | Dispositif a convection thermique pour une reaction en chaine de la polymerase |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2784150B1 (fr) |
| KR (1) | KR101691466B1 (fr) |
| CN (1) | CN103649301B (fr) |
| CA (1) | CA2856345C (fr) |
| IN (1) | IN2014CN03868A (fr) |
| WO (1) | WO2013075263A1 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016180333A1 (fr) * | 2015-05-12 | 2016-11-17 | 厦门大学 | Tube de réaction d'amplification d'acide nucléique capable de réguler la trajectoire de circulation de liquide |
| CN105670924A (zh) * | 2016-03-01 | 2016-06-15 | 上海理工大学 | 自然对流聚合酶链反应微系统 |
| CN107964507B (zh) | 2016-10-18 | 2022-09-27 | 财团法人工业技术研究院 | 热对流聚合酶连锁反应装置及其光学检测方法 |
| CN108410688A (zh) * | 2017-02-09 | 2018-08-17 | 克雷多生物医学私人有限公司 | 一种热对流式聚合酶链式反应的装置 |
| CN108949545A (zh) * | 2018-08-16 | 2018-12-07 | 上海海洋大学 | 一种新型核酸等温扩增反应组件 |
| CN109401948A (zh) * | 2018-11-01 | 2019-03-01 | 福建省博凯科技有限公司 | 一段式热对流pcr仪 |
| CN109294901A (zh) * | 2018-11-01 | 2019-02-01 | 福建省博凯科技有限公司 | 一段式热对流pcr仪及控制方法 |
| CN109554295B (zh) * | 2019-01-21 | 2022-03-29 | 武汉理工大学 | 远洋船员的pcr扩增与疾病检测装置 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2130013C (fr) * | 1993-09-10 | 1999-03-30 | Rolf Moser | Appareil servant a modifier automatiquement la temperature du contenu de differentes eprouvettes |
| KR100488281B1 (ko) * | 2001-09-15 | 2005-05-10 | 아람 바이오시스템 주식회사 | 열 대류를 이용한 염기서열 증폭 방법 및 장치 |
| DE102004039092B4 (de) * | 2004-08-11 | 2008-04-24 | Deutsches Zentrum für Luft- und Raumfahrt e.V. | Verriegelungsvorrichtung für Linearaktuatoren |
| US8153064B2 (en) * | 2007-03-22 | 2012-04-10 | Doebler Ii Robert W | Systems and devices for isothermal biochemical reactions and/or analysis |
| GB0715170D0 (en) * | 2007-08-03 | 2007-09-12 | Enigma Diagnostics Ltd | Reaction vessel |
| US20100124779A1 (en) * | 2008-11-18 | 2010-05-20 | Roche Diagnostics Operations, Inc. | Two-Step Moulded Capillary |
| CN104630056B (zh) * | 2010-01-12 | 2017-11-07 | 阿赫姆生物系统公司 | 两阶段热对流装置及其用途 |
-
2011
- 2011-11-22 KR KR1020147002887A patent/KR101691466B1/ko active IP Right Grant
- 2011-11-22 CA CA2856345A patent/CA2856345C/fr active Active
- 2011-11-22 WO PCT/CN2011/001941 patent/WO2013075263A1/fr active Application Filing
- 2011-11-22 EP EP11876152.7A patent/EP2784150B1/fr active Active
- 2011-11-22 CN CN201180072273.1A patent/CN103649301B/zh active Active
- 2011-11-22 IN IN3868CHN2014 patent/IN2014CN03868A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IN2014CN03868A (fr) | 2015-10-16 |
| WO2013075263A1 (fr) | 2013-05-30 |
| CA2856345A1 (fr) | 2013-05-30 |
| KR101691466B1 (ko) | 2016-12-30 |
| CN103649301A (zh) | 2014-03-19 |
| EP2784150A4 (fr) | 2015-08-12 |
| KR20140040262A (ko) | 2014-04-02 |
| EP2784150A1 (fr) | 2014-10-01 |
| EP2784150B1 (fr) | 2019-05-15 |
| CN103649301B (zh) | 2015-03-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20140520 |