CA2828286C - Aptamers for clostridium difficile diagnostics - Google Patents

Aptamers for clostridium difficile diagnostics Download PDF

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Publication number
CA2828286C
CA2828286C CA2828286A CA2828286A CA2828286C CA 2828286 C CA2828286 C CA 2828286C CA 2828286 A CA2828286 A CA 2828286A CA 2828286 A CA2828286 A CA 2828286A CA 2828286 C CA2828286 C CA 2828286C
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CA
Canada
Prior art keywords
toxin
aptamer
aptamers
target
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2828286A
Other languages
English (en)
French (fr)
Other versions
CA2828286A1 (en
Inventor
Urs Ochsner
Evaldas Katilius
Nebojsa Janjic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Somalogic Inc
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somalogic Inc filed Critical Somalogic Inc
Publication of CA2828286A1 publication Critical patent/CA2828286A1/en
Application granted granted Critical
Publication of CA2828286C publication Critical patent/CA2828286C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91091—Glycosyltransferases (2.4)
    • G01N2333/91097—Hexosyltransferases (general) (2.4.1)
    • G01N2333/91102—Hexosyltransferases (general) (2.4.1) with definite EC number (2.4.1.-)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2828286A 2011-03-10 2012-03-09 Aptamers for clostridium difficile diagnostics Expired - Fee Related CA2828286C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161451227P 2011-03-10 2011-03-10
US61/451,227 2011-03-10
PCT/US2012/028632 WO2012122540A1 (en) 2011-03-10 2012-03-09 Aptamers for clostridium difficile diagnostics

Publications (2)

Publication Number Publication Date
CA2828286A1 CA2828286A1 (en) 2012-09-13
CA2828286C true CA2828286C (en) 2018-04-03

Family

ID=46795908

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2828286A Expired - Fee Related CA2828286C (en) 2011-03-10 2012-03-09 Aptamers for clostridium difficile diagnostics

Country Status (8)

Country Link
US (2) US8895241B2 (https=)
EP (1) EP2683729B1 (https=)
JP (1) JP6018096B2 (https=)
KR (1) KR20140015455A (https=)
AU (2) AU2012225273B2 (https=)
CA (1) CA2828286C (https=)
ES (1) ES2629183T3 (https=)
WO (1) WO2012122540A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012225273B2 (en) 2011-03-10 2016-10-13 Somalogic, Inc. Aptamers for Clostridium difficile diagnostics
JP6506768B2 (ja) * 2013-09-24 2019-04-24 ソマロジック・インコーポレーテッド 多重アプタマー標的の検出
CA3152415A1 (en) * 2014-02-18 2015-08-27 Somalogic Operating Co., Inc. Compositions and methods for detecting microorganisms
NO2718257T3 (https=) * 2014-05-30 2018-04-14
TWI770036B (zh) 2016-07-01 2022-07-11 美商私募蛋白質體操作有限公司 包含經修飾核苷之寡核苷酸
CN106841603B (zh) * 2017-01-23 2020-11-06 郑州轻工业学院 一种利用血糖仪高灵敏定量检测黄曲霉毒素b1的方法
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
CN109507423A (zh) * 2018-12-27 2019-03-22 南京祥中生物科技有限公司 一种基于适配体探针同时检测α-乳白蛋白、β-乳球蛋白和乳铁蛋白含量的方法
KR20220041072A (ko) 2019-06-05 2022-03-31 엘아이브이 프로세스, 인크. 클로스트리듐 디피실에 대한 앱타머
KR102113078B1 (ko) * 2019-07-05 2020-05-20 광주과학기술원 압타머의 선별 방법
US11898146B2 (en) 2020-12-03 2024-02-13 Liv Process, Inc. Aptamers against Clostridium difficile, compositions comprising aptamers against Clostridium difficile and methods of using the same

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070071744A1 (en) 2002-06-07 2007-03-29 Gotz Munch Agents which bind to epitopes of glycoprotein VI
US7595160B2 (en) * 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US20100035247A1 (en) * 2005-11-04 2010-02-11 U.S. Genomics, Inc. Heterogeneous Assay of Analytes in Solution Using Polymers
US8658670B2 (en) * 2006-09-26 2014-02-25 Crestone, Inc. Methods and compounds for treatment of clostridium based infection
US7947447B2 (en) * 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
WO2009037264A2 (en) * 2007-09-17 2009-03-26 Da Volterra Colonic delivery of antimicrobial agents
US8569252B2 (en) 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
BRPI1011269A2 (pt) * 2009-05-05 2016-09-27 Altermune Technologies Llc imunidade quimicamente programável
WO2011100443A1 (en) * 2010-02-11 2011-08-18 Intelligent Medical Devices, Inc. Oligonucleotides relating to clostridium difficile genes encoding toxin b, toxin a, or binary toxin
GB201002627D0 (en) * 2010-02-16 2010-03-31 Loxbridge Res Llp Aptamer based analyte detection method
US8785132B2 (en) 2010-04-23 2014-07-22 Postech Academy-Industry Foundation Aptamer sandwich assays
AU2012225273B2 (en) 2011-03-10 2016-10-13 Somalogic, Inc. Aptamers for Clostridium difficile diagnostics
MX373248B (es) 2011-09-30 2020-05-11 Somalogic Operating Co Inc Prediccion de riesgo de evento cardiovascular y usos del mismo.

Also Published As

Publication number Publication date
CA2828286A1 (en) 2012-09-13
US8895241B2 (en) 2014-11-25
KR20140015455A (ko) 2014-02-06
AU2016256840A1 (en) 2016-12-01
AU2012225273B2 (en) 2016-10-13
AU2012225273A1 (en) 2013-09-12
WO2012122540A1 (en) 2012-09-13
JP6018096B2 (ja) 2016-11-02
EP2683729A1 (en) 2014-01-15
EP2683729A4 (en) 2014-11-12
US20120231467A1 (en) 2012-09-13
ES2629183T3 (es) 2017-08-07
JP2014513929A (ja) 2014-06-19
AU2016256840B2 (en) 2017-11-02
US20150125867A1 (en) 2015-05-07
US9081010B2 (en) 2015-07-14
EP2683729B1 (en) 2017-05-03

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20170130

MKLA Lapsed

Effective date: 20200309