CA2820715C - Universal reference dye for quantitative amplification - Google Patents

Universal reference dye for quantitative amplification Download PDF

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Publication number
CA2820715C
CA2820715C CA2820715A CA2820715A CA2820715C CA 2820715 C CA2820715 C CA 2820715C CA 2820715 A CA2820715 A CA 2820715A CA 2820715 A CA2820715 A CA 2820715A CA 2820715 C CA2820715 C CA 2820715C
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CA
Canada
Prior art keywords
dye
passive reference
reference dye
passive
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2820715A
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English (en)
French (fr)
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CA2820715A1 (en
Inventor
Yan Wang
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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Publication date
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Publication of CA2820715A1 publication Critical patent/CA2820715A1/en
Application granted granted Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2820715A 2010-12-16 2011-12-16 Universal reference dye for quantitative amplification Active CA2820715C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US42393210P 2010-12-16 2010-12-16
US61/423,932 2010-12-16
US201161435209P 2011-01-21 2011-01-21
US61/435,209 2011-01-21
US201161508453P 2011-07-15 2011-07-15
US61/508,453 2011-07-15
PCT/US2011/065617 WO2012083235A1 (en) 2010-12-16 2011-12-16 Universal reference dye for quantitative amplification

Publications (2)

Publication Number Publication Date
CA2820715A1 CA2820715A1 (en) 2012-06-21
CA2820715C true CA2820715C (en) 2020-03-31

Family

ID=46245139

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2820715A Active CA2820715C (en) 2010-12-16 2011-12-16 Universal reference dye for quantitative amplification

Country Status (5)

Country Link
US (1) US9493824B2 (enExample)
EP (1) EP2652154B1 (enExample)
JP (1) JP5985503B2 (enExample)
CA (1) CA2820715C (enExample)
WO (1) WO2012083235A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
US9523116B2 (en) 2013-02-01 2016-12-20 Bio-Rad Laboratories, Inc. Multiplexed digital assay with data exclusion for calculation of target levels
US10955419B2 (en) 2013-03-05 2021-03-23 University Of Washington Methods and systems for analysis using polymer dots
US20140356968A1 (en) * 2013-06-04 2014-12-04 Therapeutic Proteins International, LLC Methods for comparing a structure of a first biomolecule and a second biomolecule
EP2989178B1 (en) * 2013-10-10 2019-12-04 Bio-Rad Laboratories, Inc. Composite visible colorant and method for quantitative amplification
US20170002398A1 (en) * 2014-01-23 2017-01-05 Qiagen Gmbh Nucleic acid amplification method
CA3005600C (en) 2015-11-20 2021-08-10 Seegene, Inc. Method for calibrating a data set of a target analyte
US10526536B2 (en) 2015-12-22 2020-01-07 Roche Molecular Systems, Inc. Compositions with thermolabile dyes
CN111433372B (zh) 2017-10-19 2024-01-26 生物辐射实验室股份有限公司 具有光致发光的非常规和/或反向变化的数字扩增试验
JP7532820B2 (ja) * 2019-03-20 2024-08-14 東洋紡株式会社 非特異増幅が抑制された核酸増幅法
EP4419711A1 (en) * 2021-10-20 2024-08-28 Qiagen GmbH High stokes shift fluorescence dyes for multiplex detection
CN119462681B (zh) * 2024-10-16 2025-09-30 聊城柏莱斯生物科技有限公司 一种荧光定量pcr参比染料及其制备方法与应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US6028190A (en) * 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5928927A (en) 1996-04-16 1999-07-27 The United States Of America As Represented By The Secretary Of The Army Enzymatic detoxification of organophosphorus compounds
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US7498164B2 (en) * 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
US7410793B2 (en) * 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
US7166478B2 (en) 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US20060002474A1 (en) * 2004-06-26 2006-01-05 Oscar Chi-Lim Au Efficient multi-block motion estimation for video compression
JP2006101766A (ja) * 2004-10-05 2006-04-20 Plant Genome Center Co Ltd 遺伝子の発現レベルを指標とした穀物の食味判定方法
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7465561B2 (en) 2005-06-30 2008-12-16 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using single probe analysis
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
US20080001099A1 (en) * 2006-07-01 2008-01-03 Sharaf Muhammad A Quantitative calibration method and system for genetic analysis instrumentation
EP2099930B1 (en) 2006-12-13 2015-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US7872116B2 (en) 2007-02-06 2011-01-18 American Type Culture Collection (Atcc) Identification of cell culture contaminants among Mollicutes species by a PCR based assay
EP2217719B1 (en) * 2007-10-22 2011-07-13 LGC Limited Oligonucleotides and uses thereof
BRPI0908748A2 (pt) 2008-03-15 2015-07-28 Hologic Inc Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação
EP2192198A1 (en) * 2008-12-01 2010-06-02 Biotype AG Novel combination of fluorescent dyes for the detection of nucleic acids
WO2010107816A2 (en) 2009-03-16 2010-09-23 Promega Corporation Nucleic acid binding dyes and uses therefor
US8916352B2 (en) * 2011-04-08 2014-12-23 Bio-Rad Laboratories, Inc. PCR reaction mixtures with decreased non-specific activity

Also Published As

Publication number Publication date
EP2652154A1 (en) 2013-10-23
JP2014502496A (ja) 2014-02-03
CA2820715A1 (en) 2012-06-21
US20120164690A1 (en) 2012-06-28
JP5985503B2 (ja) 2016-09-06
EP2652154B1 (en) 2017-10-18
EP2652154A4 (en) 2014-12-31
US9493824B2 (en) 2016-11-15
WO2012083235A1 (en) 2012-06-21

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