CA2820715C - Universal reference dye for quantitative amplification - Google Patents
Universal reference dye for quantitative amplification Download PDFInfo
- Publication number
- CA2820715C CA2820715C CA2820715A CA2820715A CA2820715C CA 2820715 C CA2820715 C CA 2820715C CA 2820715 A CA2820715 A CA 2820715A CA 2820715 A CA2820715 A CA 2820715A CA 2820715 C CA2820715 C CA 2820715C
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- Prior art keywords
- dye
- passive reference
- reference dye
- passive
- mixture
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
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| PCT/US2011/065617 WO2012083235A1 (en) | 2010-12-16 | 2011-12-16 | Universal reference dye for quantitative amplification |
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| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| WO2014121240A1 (en) * | 2013-02-01 | 2014-08-07 | Bio-Rad Laboratories, Inc. | Multiplexed digital assay with specific and generic reporters |
| EP2964565B1 (en) * | 2013-03-05 | 2019-12-04 | University Of Washington Through Its Center For Commercialization | Western blot analysis using polymer dots |
| US20140356968A1 (en) * | 2013-06-04 | 2014-12-04 | Therapeutic Proteins International, LLC | Methods for comparing a structure of a first biomolecule and a second biomolecule |
| WO2015054396A1 (en) | 2013-10-10 | 2015-04-16 | Bio-Rad Laboratories, Inc. | Composite visible colorant and method for quantitative amplification |
| US20170002398A1 (en) * | 2014-01-23 | 2017-01-05 | Qiagen Gmbh | Nucleic acid amplification method |
| KR102270771B1 (ko) | 2015-11-20 | 2021-06-29 | 주식회사 씨젠 | 표적 분석물질에 대한 데이터 세트의 보정 방법 |
| CN108368495B (zh) * | 2015-12-22 | 2022-05-13 | 豪夫迈·罗氏有限公司 | 具有不耐热染料的组合物 |
| EP3697927B1 (en) | 2017-10-19 | 2022-12-14 | Bio-Rad Laboratories, Inc. | Digital amplification assays with unconventional and/or inverse changes in photoluminescence |
| JP7532820B2 (ja) * | 2019-03-20 | 2024-08-14 | 東洋紡株式会社 | 非特異増幅が抑制された核酸増幅法 |
| WO2023067077A1 (en) * | 2021-10-20 | 2023-04-27 | Qiagen Gmbh | High stokes shift fluorescence dyes for multiplex detection |
| CN119462681B (zh) * | 2024-10-16 | 2025-09-30 | 聊城柏莱斯生物科技有限公司 | 一种荧光定量pcr参比染料及其制备方法与应用 |
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| US5928927A (en) | 1996-04-16 | 1999-07-27 | The United States Of America As Represented By The Secretary Of The Army | Enzymatic detoxification of organophosphorus compounds |
| US5736333A (en) | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
| US7498164B2 (en) * | 1998-05-16 | 2009-03-03 | Applied Biosystems, Llc | Instrument for monitoring nucleic acid sequence amplification reaction |
| US7410793B2 (en) * | 1999-05-17 | 2008-08-12 | Applera Corporation | Optical instrument including excitation source |
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| JP2006101766A (ja) * | 2004-10-05 | 2006-04-20 | Plant Genome Center Co Ltd | 遺伝子の発現レベルを指標とした穀物の食味判定方法 |
| US7776567B2 (en) | 2005-03-17 | 2010-08-17 | Biotium, Inc. | Dimeric and trimeric nucleic acid dyes, and associated systems and methods |
| US8067208B2 (en) | 2005-06-30 | 2011-11-29 | Roche Molecular Systems, Inc. | Probes and methods for hepatitis C virus typing using multidimensional probe analysis |
| US7785786B2 (en) | 2006-01-23 | 2010-08-31 | Quest Diagnostics Investments Incorporated | Methods for detecting nucleic acids using multiple signals |
| US20080001099A1 (en) * | 2006-07-01 | 2008-01-03 | Sharaf Muhammad A | Quantitative calibration method and system for genetic analysis instrumentation |
| KR101433208B1 (ko) | 2006-12-13 | 2014-08-22 | 루미넥스 코포레이션 | 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법 |
| US7872116B2 (en) | 2007-02-06 | 2011-01-18 | American Type Culture Collection (Atcc) | Identification of cell culture contaminants among Mollicutes species by a PCR based assay |
| CA2703227C (en) | 2007-10-22 | 2017-02-28 | Lgc Limited | Oligonucleotides and uses thereof |
| KR101419980B1 (ko) | 2008-03-15 | 2014-07-15 | 홀로직, 인크. | 증폭 반응 도중에 핵산 분자를 분석하기 위한 조성물 및 방법 |
| EP2192198A1 (en) * | 2008-12-01 | 2010-06-02 | Biotype AG | Novel combination of fluorescent dyes for the detection of nucleic acids |
| US8598198B2 (en) | 2009-03-16 | 2013-12-03 | Promega Corporation | Nucleic acid binding dyes and uses therefor |
| CN103703141B (zh) * | 2011-04-08 | 2016-08-17 | 伯乐实验室公司 | 具有降低的非特异性活性的pcr反应混合物 |
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| EP2652154B1 (en) | 2017-10-18 |
| WO2012083235A1 (en) | 2012-06-21 |
| US9493824B2 (en) | 2016-11-15 |
| EP2652154A1 (en) | 2013-10-23 |
| JP2014502496A (ja) | 2014-02-03 |
| US20120164690A1 (en) | 2012-06-28 |
| JP5985503B2 (ja) | 2016-09-06 |
| EP2652154A4 (en) | 2014-12-31 |
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