CA2768462A1 - Improved anti-serum albumin binding single variable domains - Google Patents
Improved anti-serum albumin binding single variable domains Download PDFInfo
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- CA2768462A1 CA2768462A1 CA2768462A CA2768462A CA2768462A1 CA 2768462 A1 CA2768462 A1 CA 2768462A1 CA 2768462 A CA2768462 A CA 2768462A CA 2768462 A CA2768462 A CA 2768462A CA 2768462 A1 CA2768462 A1 CA 2768462A1
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C07K—PEPTIDES
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- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/31—Fusion polypeptide fusions, other than Fc, for prolonged plasma life, e.g. albumin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Organic Chemistry (AREA)
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- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
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| US22602809P | 2009-07-16 | 2009-07-16 | |
| US61/226,028 | 2009-07-16 | ||
| US30755410P | 2010-02-24 | 2010-02-24 | |
| US61/307,554 | 2010-02-24 | ||
| PCT/EP2010/060112 WO2011006915A2 (en) | 2009-07-16 | 2010-07-14 | Improved anti-serum albumin binding single variable domains |
Publications (1)
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|---|---|
| CA2768462A1 true CA2768462A1 (en) | 2011-01-20 |
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| CA2768462A Abandoned CA2768462A1 (en) | 2009-07-16 | 2010-07-14 | Improved anti-serum albumin binding single variable domains |
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|---|---|
| US (2) | US8679496B2 (enExample) |
| EP (1) | EP2454285A2 (enExample) |
| JP (1) | JP2012532620A (enExample) |
| KR (1) | KR20120038494A (enExample) |
| CN (1) | CN102574914A (enExample) |
| AU (1) | AU2010272590B2 (enExample) |
| BR (1) | BR112012001681A2 (enExample) |
| CA (1) | CA2768462A1 (enExample) |
| EA (1) | EA201270174A1 (enExample) |
| IL (1) | IL217520A0 (enExample) |
| IN (1) | IN2012DN00640A (enExample) |
| MX (1) | MX2012000765A (enExample) |
| SG (1) | SG177601A1 (enExample) |
| WO (1) | WO2011006915A2 (enExample) |
| ZA (1) | ZA201200276B (enExample) |
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| KR20110119806A (ko) * | 2009-02-19 | 2011-11-02 | 글락소 그룹 리미티드 | 개선된 항ｔｎｆｒ1 폴리펩티드，항체 가변 도메인 및 길항제 |
| JP2012532620A (ja) * | 2009-07-16 | 2012-12-20 | グラクソ グループ リミテッド | 改良型抗血清アルブミン結合単一可変ドメイン |
| EP2603522A1 (en) | 2010-08-13 | 2013-06-19 | GlaxoSmithKline Intellectual Property Development Limited | Improved anti-serum albumin binding variants |
| US20130266567A1 (en) * | 2010-12-01 | 2013-10-10 | Haren Arulanantham | Anti-serum albumin binding single variable domains |
| MX351502B (es) * | 2011-11-11 | 2017-10-18 | Ucb Pharma Sa | Anticuerpos de union de albumina y fragmentos de union de los mismos. |
| GB201200563D0 (en) * | 2012-01-13 | 2012-02-29 | Imp Innovations Ltd | Binding molecule |
| WO2017041114A2 (en) * | 2015-09-03 | 2017-03-09 | The Children's Mercy Hospital | Anti-nicotinamide phosphoribosyltransferase antibody genes and methods of use thereof |
| NO2768984T3 (enExample) | 2015-11-12 | 2018-06-09 | ||
| EP3974449A1 (en) * | 2015-11-13 | 2022-03-30 | Ablynx NV | Improved serum albumin-binding immunoglobulin variable domains |
| JP7046804B2 (ja) | 2015-11-18 | 2022-04-04 | アブリンクス エン．ヴェー． | 改良された血清アルブミン結合剤 |
| TWI754622B (zh) | 2015-11-18 | 2022-02-11 | 美商默沙東藥廠 | Ctla4結合劑 |
| CA3003777A1 (en) | 2015-11-18 | 2017-05-26 | Merck Sharp & Dohme Corp. | Pd1/ctla4 binders |
| HK1254951A1 (zh) | 2015-11-18 | 2019-08-02 | Merck Sharp & Dohme Llc | Pd1和/或lag3结合剂 |
| CN115837014A (zh) | 2016-05-18 | 2023-03-24 | 摩登纳特斯有限公司 | 编码松弛素的多核苷酸 |
| CN110049997B (zh) | 2016-12-07 | 2023-09-22 | 埃博灵克斯股份有限公司 | 改进的血清白蛋白结合免疫球蛋白单可变结构域 |
| ES2991459T3 (es) | 2017-01-17 | 2024-12-03 | Ablynx Nv | Ligantes de albúmina sérica mejorados |
| EP3571224B1 (en) | 2017-01-17 | 2024-08-07 | Ablynx NV | Improved serum albumin binders |
| AU2018220516B2 (en) | 2017-02-16 | 2025-03-06 | Sonnet BioTherapeutics, Inc. | Albumin binding domain fusion proteins |
| PE20200849A1 (es) | 2017-10-10 | 2020-08-20 | Numab Therapeutics AG | Anticuerpo multiespecifico |
| EP3470426A1 (en) | 2017-10-10 | 2019-04-17 | Numab Therapeutics AG | Multispecific antibody |
| CN113508134B (zh) | 2019-02-22 | 2024-12-03 | 安维达生物科技公司 | 白蛋白结合抗体及其用途 |
| EP3816185A1 (en) | 2019-11-04 | 2021-05-05 | Numab Therapeutics AG | Multispecific antibody directed against pd-l1 and a tumor-associated antigen |
| GB201919061D0 (en) | 2019-12-20 | 2020-02-05 | Ucb Biopharma Sprl | Multi-specific antibody |
| EP4133084A1 (en) | 2020-04-09 | 2023-02-15 | Autolus Limited | Polypeptide |
| GB202007441D0 (en) | 2020-05-19 | 2020-07-01 | Autolus Ltd | Polypeptide |
| EP3915580A1 (en) | 2020-05-29 | 2021-12-01 | Numab Therapeutics AG | Multispecific antibody |
| IT202000013006A1 (it) * | 2020-06-01 | 2021-12-01 | Bioreweal S R L | Processo per la produzione biologica di idrogeno e/o di metano mediante assorbimento e conversione biologica di anidride carbonica. |
| EP3988568A1 (en) | 2020-10-21 | 2022-04-27 | Numab Therapeutics AG | Combination treatment |
| PE20231953A1 (es) | 2020-12-07 | 2023-12-06 | UCB Biopharma SRL | Anticuerpos multiespecificos y combinaciones de anticuerpos |
| US20240392003A1 (en) | 2021-02-02 | 2024-11-28 | Numab Therapeutics AG | Multispecific antibodies having specificity for ror1 and cd3 |
| EP4183800A1 (en) | 2021-11-19 | 2023-05-24 | Medizinische Hochschule Hannover | Novel sars-cov-2 neutralizing antibodies |
| WO2024038095A1 (en) | 2022-08-16 | 2024-02-22 | Iome Bio | NOVEL ANTI-RGMb ANTIBODIES |
| IL322519A (en) | 2023-02-17 | 2025-10-01 | Ablynx Nv | Polypeptides that bind to the neonatal fc receptor |
| WO2025061919A1 (en) | 2023-09-22 | 2025-03-27 | Ablynx Nv | Bi- and multivalent albumin binders |
| US12134635B1 (en) | 2023-12-29 | 2024-11-05 | Sonnet BioTherapeutics, Inc. | Interleukin 18 (IL-18) variants and fusion proteins comprising same |
| US12459994B2 (en) | 2024-04-12 | 2025-11-04 | Paragon Therapeutics, Inc. | Albumin binding proteins and methods of use |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL127127A0 (en) | 1998-11-18 | 1999-09-22 | Peptor Ltd | Small functional units of antibody heavy chain variable regions |
| AU2002314495A1 (en) * | 2001-06-20 | 2003-01-02 | Prochon Biotech Ltd. | Antibodies that block receptor protein tyrosine kinase activation, methods of screening for and uses thereof |
| US20100081792A1 (en) * | 2001-06-28 | 2010-04-01 | Smithkline Beecham Corporation | Ligand |
| US9321832B2 (en) * | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
| EP1600459A3 (en) | 2002-06-28 | 2005-12-07 | Domantis Limited | Ligand |
| EP1737962B1 (en) | 2004-03-24 | 2010-09-01 | Domantis Limited | Gas1 universal leader |
| US8921528B2 (en) | 2004-06-01 | 2014-12-30 | Domantis Limited | Bispecific fusion antibodies with enhanced serum half-life |
| US7563443B2 (en) * | 2004-09-17 | 2009-07-21 | Domantis Limited | Monovalent anti-CD40L antibody polypeptides and compositions thereof |
| GB0521621D0 (en) * | 2005-10-24 | 2005-11-30 | Domantis Ltd | Tumor necrosis factor receptor 1 antagonists for treating respiratory diseases |
| BRPI0518761A2 (pt) | 2004-12-02 | 2008-12-09 | Domantis Ltd | fusço de droga, conjugado de droga, Ácido nucleico recombinante, construÇço de Ácido nucleico, cÉlula hospedeira, mÉtodo para produzir uma fusço de droga, composiÇço farmacÊutica, droga, mÉtodo de tratamento e/ou prevenÇço de uma condiÇço em um paciente, mÉtodo de retardo ou prevenÇço de progressço de doenÇa, e, mÉtodo para diminuir a absorÇço de alimentos por um paciente |
| CN101466734A (zh) * | 2005-12-01 | 2009-06-24 | 杜门蒂斯有限公司 | 与白细胞介素-1受体1型结合的竞争性域抗体形式 |
| KR20080077238A (ko) * | 2005-12-01 | 2008-08-21 | 도만티스 리미티드 | 인터루킨 1 수용체 타입 1에 결합하는 비경쟁적 도메인항체 포맷 |
| TW200804425A (en) * | 2005-12-06 | 2008-01-16 | Domantis Ltd | Ligands that have binding specificity for EGFR and/or VEGF and methods of use therefor |
| US20100047171A1 (en) | 2006-01-24 | 2010-02-25 | Roland Beckmann | Fusion Proteins That Contain Natural Junctions |
| EP1976882A2 (en) * | 2006-01-24 | 2008-10-08 | Domantis Limited | Ligands that bind il-4 and/or il-13 |
| AU2008259590A1 (en) | 2007-06-06 | 2008-12-11 | Domantis Limited | Methods for selecting protease resistant polypeptides |
| WO2009149143A1 (en) * | 2008-06-03 | 2009-12-10 | E. I. Du Pont De Nemours And Company | Adhesion of polyamides to epoxy resins |
| US8906035B2 (en) * | 2008-06-04 | 2014-12-09 | Ethicon Endo-Surgery, Inc. | Endoscopic drop off bag |
| EP2297315A2 (en) * | 2008-06-06 | 2011-03-23 | Danisco US Inc. | Compositions and methods comprising variant microbial proteases |
| US10198891B2 (en) * | 2008-06-06 | 2019-02-05 | Epona Llc | System and method for regulating fuel transactions |
| JP2012532620A (ja) * | 2009-07-16 | 2012-12-20 | グラクソ グループ リミテッド | 改良型抗血清アルブミン結合単一可変ドメイン |
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| US20120114647A1 (en) | 2012-05-10 |
| MX2012000765A (es) | 2012-02-13 |
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| BR112012001681A2 (pt) | 2019-09-24 |
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| EP2454285A2 (en) | 2012-05-23 |
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| IN2012DN00640A (enExample) | 2015-08-21 |
| JP2012532620A (ja) | 2012-12-20 |
| US20140140996A1 (en) | 2014-05-22 |
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| AU2010272590B2 (en) | 2013-10-10 |
| WO2011006915A3 (en) | 2011-03-03 |
| US8679496B2 (en) | 2014-03-25 |
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