CA2750474C - Fast reaction kinetics of enzymes having low activity in dry chemistry layers - Google Patents

Fast reaction kinetics of enzymes having low activity in dry chemistry layers Download PDF

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Publication number
CA2750474C
CA2750474C CA2750474A CA2750474A CA2750474C CA 2750474 C CA2750474 C CA 2750474C CA 2750474 A CA2750474 A CA 2750474A CA 2750474 A CA2750474 A CA 2750474A CA 2750474 C CA2750474 C CA 2750474C
Authority
CA
Canada
Prior art keywords
glucose dehydrogenase
analyte
dehydrogenase
mutated
case independently
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2750474A
Other languages
English (en)
French (fr)
Other versions
CA2750474A1 (en
Inventor
Carina Horn
Claudia Gassler-Dietsche
Dieter Heindl
Joachim Hones
Thomas Meier
Rainer Schmuck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2009/001206 external-priority patent/WO2009103540A1/de
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2750474A1 publication Critical patent/CA2750474A1/en
Application granted granted Critical
Publication of CA2750474C publication Critical patent/CA2750474C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2750474A 2009-02-19 2010-02-12 Fast reaction kinetics of enzymes having low activity in dry chemistry layers Expired - Fee Related CA2750474C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EPPCT/EP2009/001206 2009-02-19
PCT/EP2009/001206 WO2009103540A1 (de) 2008-02-19 2009-02-19 Stabilisierung von dehydrogenasen mit stabilen coenzymen
PCT/EP2010/051801 WO2010094632A1 (de) 2009-02-19 2010-02-12 Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten

Publications (2)

Publication Number Publication Date
CA2750474A1 CA2750474A1 (en) 2010-08-26
CA2750474C true CA2750474C (en) 2017-07-25

Family

ID=42112222

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2750474A Expired - Fee Related CA2750474C (en) 2009-02-19 2010-02-12 Fast reaction kinetics of enzymes having low activity in dry chemistry layers

Country Status (3)

Country Link
KR (2) KR101604624B1 (de)
CA (1) CA2750474C (de)
WO (1) WO2010094632A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2398909B1 (de) * 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
WO2012101095A1 (en) 2011-01-26 2012-08-02 Roche Diagnostics Gmbh Method and substances for preparation of n-substituted pyridinium compounds
KR101700022B1 (ko) 2012-06-22 2017-02-13 에프. 호프만-라 로슈 아게 체액에서 분석물질을 검출하는 방법 및 디바이스
CN105247069A (zh) 2013-06-04 2016-01-13 豪夫迈·罗氏有限公司 用于fret的新化合物及与其相关的方法
JP2019529935A (ja) 2016-10-05 2019-10-17 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 多検体診断用試験エレメントのための検出試薬および電極配置、ならびにそれらを使用する方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801006A (en) 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
AU2004288134B2 (en) 2003-08-11 2009-12-17 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides
PL2705792T3 (pl) 2004-03-06 2015-10-30 Hoffmann La Roche Urządzenie do pobierania próbki płynu ustrojowego
ATE528652T1 (de) 2005-06-29 2011-10-15 Hoffmann La Roche Analysesystem mit testband
DE102005035461A1 (de) 2005-07-28 2007-02-15 Roche Diagnostics Gmbh Stabile NAD/NADH-Derivate
EP2093284A1 (de) * 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen

Also Published As

Publication number Publication date
KR20130127555A (ko) 2013-11-22
WO2010094632A1 (de) 2010-08-26
CA2750474A1 (en) 2010-08-26
KR20110117166A (ko) 2011-10-26
KR101604624B1 (ko) 2016-03-28

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Effective date: 20200212