CA2750474C - Cinetique reactionnelle rapide d'enzymes presentant une activite inferieure dans des couches chimiques seches - Google Patents

Cinetique reactionnelle rapide d'enzymes presentant une activite inferieure dans des couches chimiques seches Download PDF

Info

Publication number
CA2750474C
CA2750474C CA2750474A CA2750474A CA2750474C CA 2750474 C CA2750474 C CA 2750474C CA 2750474 A CA2750474 A CA 2750474A CA 2750474 A CA2750474 A CA 2750474A CA 2750474 C CA2750474 C CA 2750474C
Authority
CA
Canada
Prior art keywords
glucose dehydrogenase
analyte
dehydrogenase
mutated
case independently
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2750474A
Other languages
English (en)
Other versions
CA2750474A1 (fr
Inventor
Carina Horn
Claudia Gassler-Dietsche
Dieter Heindl
Joachim Hones
Thomas Meier
Rainer Schmuck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2009/001206 external-priority patent/WO2009103540A1/fr
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2750474A1 publication Critical patent/CA2750474A1/fr
Application granted granted Critical
Publication of CA2750474C publication Critical patent/CA2750474C/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2750474A 2009-02-19 2010-02-12 Cinetique reactionnelle rapide d'enzymes presentant une activite inferieure dans des couches chimiques seches Expired - Fee Related CA2750474C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EPPCT/EP2009/001206 2009-02-19
PCT/EP2009/001206 WO2009103540A1 (fr) 2008-02-19 2009-02-19 Stabilisation de déshydrogénases avec des coenzymes stables
PCT/EP2010/051801 WO2010094632A1 (fr) 2009-02-19 2010-02-12 Cinétique réactionnelle rapide d'enzymes présentant une activité inférieure dans des couches chimiques sèches

Publications (2)

Publication Number Publication Date
CA2750474A1 CA2750474A1 (fr) 2010-08-26
CA2750474C true CA2750474C (fr) 2017-07-25

Family

ID=42112222

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2750474A Expired - Fee Related CA2750474C (fr) 2009-02-19 2010-02-12 Cinetique reactionnelle rapide d'enzymes presentant une activite inferieure dans des couches chimiques seches

Country Status (3)

Country Link
KR (2) KR20130127555A (fr)
CA (1) CA2750474C (fr)
WO (1) WO2010094632A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2398909B1 (fr) * 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Cinétique réactionnelle rapide d'enzymes présentant une activité inférieure dans des couches chimiques sèches
WO2012101095A1 (fr) 2011-01-26 2012-08-02 Roche Diagnostics Gmbh Procédé et substances permettant de préparer des composés de pyridinium n-substitués
BR112014028616B1 (pt) 2012-06-22 2020-12-29 F. Hoffmann-La Roche Ag método para a detecção de pelo menos um analito em pelo menos uma amostra de um fluido do corpo, dispositivo e sistema de teste
JP2016528175A (ja) 2013-06-04 2016-09-15 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｆｒｅｔに有用な新規化合物およびそれに関連する方法
WO2018067235A1 (fr) 2016-10-05 2018-04-12 Roche Diabetes Care, Inc. Réactifs de détection et agencements d'électrodes pour éléments de test de diagnostic multi-analytes, ainsi que leurs procédés d'utilisation

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801006A (en) 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
CA2535147A1 (fr) 2003-08-11 2005-05-19 Codexis, Inc. Polypeptides de glucose deshydrogenase et polynucleotides apparentes
HUE025706T2 (en) 2004-03-06 2016-04-28 Hoffmann La Roche Body fluid sampling device
EP1739432B1 (fr) 2005-06-29 2011-10-12 F. Hoffmann-La Roche AG Système d'analyse avec bande de test
DE102005035461A1 (de) * 2005-07-28 2007-02-15 Roche Diagnostics Gmbh Stabile NAD/NADH-Derivate
EP2093284A1 (fr) * 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisation de déhydrogénases à l'aide de coenzymes stables

Also Published As

Publication number Publication date
KR101604624B1 (ko) 2016-03-28
KR20110117166A (ko) 2011-10-26
KR20130127555A (ko) 2013-11-22
WO2010094632A1 (fr) 2010-08-26
CA2750474A1 (fr) 2010-08-26

Similar Documents

Publication Publication Date Title
JP6113405B2 (ja) 乾式試薬層において低い活性を有する、速い反応速度の酵素
KR101554457B1 (ko) 안정한 조효소를 사용하는 효소 안정화
JP6084981B2 (ja) フラビンアデニンジヌクレオチド依存型グルコース脱水素酵素活性を有するタンパク質
CA2750474C (fr) Cinetique reactionnelle rapide d'enzymes presentant une activite inferieure dans des couches chimiques seches
JP5212996B2 (ja) 生体試料中のｌ−トリプトファン分析方法およびそれに用いるキット
US9359634B2 (en) Fast reaction kinetics of enzymes having low activity in dry chemistry layers
US20200087633A1 (en) Glucose dehydrogenase variants with improved properties
Masakari et al. Comparison of the stability of Mucor-derived flavin adenine dinucleotide-dependent glucose dehydrogenase and glucose oxidase
AU2012203453B2 (en) Stabilization of dehydrogenases using stable coenzymes
NZ587397A (en) Stabilization of dehydrogenases using stable chemically modified coenzymes

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20200212