CA2744153C - Compositions and methods for the assembly of polynucleotides - Google Patents
Compositions and methods for the assembly of polynucleotides Download PDFInfo
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- CA2744153C CA2744153C CA2744153A CA2744153A CA2744153C CA 2744153 C CA2744153 C CA 2744153C CA 2744153 A CA2744153 A CA 2744153A CA 2744153 A CA2744153 A CA 2744153A CA 2744153 C CA2744153 C CA 2744153C
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- nucleic acid
- annealable linker
- linker sequence
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- General Chemical & Material Sciences (AREA)
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- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| US16223009P | 2009-03-20 | 2009-03-20 | |
| US61/162,230 | 2009-03-20 | ||
| PCT/US2009/065048 WO2010059763A1 (en) | 2008-11-19 | 2009-11-19 | Compositions and methods for the assembly of polynucleotides |
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| CA2744153A1 CA2744153A1 (en) | 2010-05-27 |
| CA2744153C true CA2744153C (en) | 2020-07-07 |
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| CA2744153A Active CA2744153C (en) | 2008-11-19 | 2009-11-19 | Compositions and methods for the assembly of polynucleotides |
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| EP (1) | EP2358875B1 (OSRAM) |
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| WO (1) | WO2010059763A1 (OSRAM) |
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| SG171760A1 (en) * | 2008-11-19 | 2011-07-28 | Amyris Inc | Compositions and methods for the assembly of polynucleotides |
| US7919605B1 (en) | 2010-08-30 | 2011-04-05 | Amyris, Inc. | Nucleic acids, compositions and methods for the excision of target nucleic acids |
| PT2702160T (pt) | 2011-04-27 | 2020-07-30 | Amyris Inc | Métodos para modificação genómica |
| BR112014010750B1 (pt) | 2011-11-09 | 2021-09-21 | Amyris, Inc | Produção de isoprenoides derivados de acetil-coenzima a |
| US8332160B1 (en) | 2011-11-17 | 2012-12-11 | Amyris Biotechnologies, Inc. | Systems and methods for engineering nucleic acid constructs using scoring techniques |
| WO2013106868A1 (en) * | 2012-01-13 | 2013-07-18 | Chromatin, Inc. | Engineering plants with rate limiting farnesene metabolic genes |
| WO2013144257A1 (en) * | 2012-03-27 | 2013-10-03 | Dsm Ip Assets B.V. | Cloning method |
| DK2867361T3 (da) * | 2012-06-29 | 2018-01-29 | Massachusetts Inst Technology | Omfangsrig parallel kombinatorisk genetik |
| US9976162B2 (en) | 2012-12-10 | 2018-05-22 | Agilent Technologies, Inc. | Pairing code directed assembly |
| EP2971027B1 (en) | 2013-03-15 | 2019-01-30 | Amyris, Inc. | Use of phosphoketolase and phosphotransacetylase for production of acetyl-coenzyme a derived compounds |
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| WO2015120403A1 (en) | 2014-02-08 | 2015-08-13 | The Regents Of The University Of Colorado, A Body Corporate | Multiplexed linking pcr |
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| WO2017024173A1 (en) | 2015-08-04 | 2017-02-09 | The Regents Of The University Of Colorado | Multiplexed binary assembly and quantitative tracking of bacterial populations |
| CN108779468A (zh) | 2015-10-29 | 2018-11-09 | 阿迈瑞斯公司 | 生成月桂烯的组合物和方法 |
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| JP2019519241A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | グルコース透過酵素ライブラリーを生成するための方法およびその使用 |
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| CA3049126A1 (en) | 2017-01-25 | 2018-08-02 | Amyris Bio Products Portugal, Unipessoal, Lda | Co-production of isoprenoids |
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| CN111386343A (zh) | 2017-09-18 | 2020-07-07 | 阿迈瑞斯公司 | 用于克鲁维酵母宿主细胞基因组整合的方法 |
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| WO2019064242A1 (en) * | 2017-09-29 | 2019-04-04 | Victoria Link Limited | MODULAR DNA ASSEMBLY SYSTEM |
| JP6985508B2 (ja) | 2017-09-30 | 2021-12-22 | インスクリプタ， インコーポレイテッド | フロースルーエレクトロポレーション機器編成 |
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| EP3951028A4 (en) * | 2019-04-01 | 2023-01-18 | National University Corporation Kobe University | METHOD OF PRODUCTION OF A CHIMERIC PLASMID LIBRARY |
| CN114008070A (zh) | 2019-06-21 | 2022-02-01 | 因思科瑞普特公司 | 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变 |
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| JP2022539879A (ja) | 2019-07-11 | 2022-09-13 | クララ フーズ カンパニー | タンパク質組成物およびその消費可能な製品 |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
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| JP7473953B2 (ja) * | 2020-03-24 | 2024-04-24 | 国立大学法人 東京大学 | 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ |
| US20230159955A1 (en) * | 2020-04-16 | 2023-05-25 | Zymergen Inc. | Circular-permuted nucleic acids for homology-directed editing |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
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| CN112813103A (zh) * | 2021-01-28 | 2021-05-18 | 云舟生物科技（广州）有限公司 | 一种标准品载体及其在测定重组腺相关病毒滴度中的应用 |
| CN113943666B (zh) * | 2021-11-11 | 2023-08-22 | 广西壮族自治区农业科学院 | 一种酿酒酵母工程菌及其应用 |
| CN115261377B (zh) * | 2021-12-27 | 2024-11-19 | 天津大学 | 一种dna串联重复单元的拆分组装方法 |
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| US5057419A (en) * | 1988-09-22 | 1991-10-15 | Rutgers University | Genetically engineered plasmid and organisms for the production of specialized oils |
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| AU5557400A (en) * | 1999-03-18 | 2001-01-31 | Complete Genomics As | Methods of cloning and producing fragment chains with readable information content |
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| US20020165175A1 (en) * | 2001-04-17 | 2002-11-07 | Xiaowu Liang | Fast and enzymeless cloning of nucleic acid fragments |
| CN1263860C (zh) | 2002-09-30 | 2006-07-12 | 华南农业大学 | 多基因载体的构建方法与应用 |
| US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| ATE483802T1 (de) * | 2005-08-11 | 2010-10-15 | Synthetic Genomics Inc | Verfahren zur in vitro-rekombination |
| WO2007021944A2 (en) * | 2005-08-11 | 2007-02-22 | The J. Craig Venter Institute | In vitro recombination method |
| US20080287320A1 (en) * | 2006-10-04 | 2008-11-20 | Codon Devices | Libraries and their design and assembly |
| AU2008212907A1 (en) | 2007-02-05 | 2008-08-14 | Philipps-Universitat Marburg | Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases |
| CN101932725A (zh) * | 2007-10-08 | 2010-12-29 | 合成基因组股份有限公司 | 大核酸的装配 |
| SG171760A1 (en) * | 2008-11-19 | 2011-07-28 | Amyris Inc | Compositions and methods for the assembly of polynucleotides |
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