CA2727535A1 - Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same - Google Patents

Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same Download PDF

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Publication number
CA2727535A1
CA2727535A1 CA2727535A CA2727535A CA2727535A1 CA 2727535 A1 CA2727535 A1 CA 2727535A1 CA 2727535 A CA2727535 A CA 2727535A CA 2727535 A CA2727535 A CA 2727535A CA 2727535 A1 CA2727535 A1 CA 2727535A1
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CA
Canada
Prior art keywords
cells
hepatic
layer
human
matrix components
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2727535A
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English (en)
French (fr)
Inventor
Lola M. Reid
Randall E. Mcclelland
Joshua Uronis
Hsin-Lei Yao
Eliane Wauthier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of North Carolina at Chapel Hill
Original Assignee
University of North Carolina at Chapel Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of North Carolina at Chapel Hill filed Critical University of North Carolina at Chapel Hill
Publication of CA2727535A1 publication Critical patent/CA2727535A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2727535A 2007-06-15 2008-06-13 Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same Abandoned CA2727535A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94443507P 2007-06-15 2007-06-15
US60/944,435 2007-06-15
PCT/US2008/007397 WO2008156667A1 (en) 2007-06-15 2008-06-13 Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same

Publications (1)

Publication Number Publication Date
CA2727535A1 true CA2727535A1 (en) 2008-12-24

Family

ID=40136896

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2727535A Abandoned CA2727535A1 (en) 2007-06-15 2008-06-13 Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same

Country Status (13)

Country Link
US (2) US20080318316A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP2171040A4 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JP2010529855A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (1) KR20100094970A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (1) CN101868533A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU2008266992A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (1) BRPI0813353A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA2727535A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IL (1) IL202738A0 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2010KN00157A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
MX (1) MX2009013819A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
TW (1) TW200916581A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2008156667A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
CA2779144C (en) * 2009-10-30 2022-10-25 The University Of North Carolina At Chapel Hill Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same
NZ603913A (en) * 2010-05-07 2015-09-25 Univ North Carolina Method of engrafting cells from solid tissues
ITMI20120896A1 (it) * 2012-05-23 2013-11-24 Bongulielmi Reto Condroitina per uso in medicina
KR20150084015A (ko) * 2012-11-08 2015-07-21 인제네론 인코포레이티드 재생 세포의 배양, 보존, 및 투여용 배지
US9533013B2 (en) 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
US11066649B2 (en) 2014-03-19 2021-07-20 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for inducing human cholangiocyte differentiation
WO2016010082A1 (ja) * 2014-07-16 2016-01-21 国立大学法人大阪大学 ラミニンフラグメントの細胞培養基質活性増強方法
CN108431209B (zh) * 2015-06-12 2023-02-10 新加坡科技研究局 肝脏干细胞的衍生和成熟肝细胞类型及其用途
US20170369849A1 (en) 2016-05-11 2017-12-28 The University Of North Carolina At Chapel Hill Compositions and methods for bioengineered tissues
JP2020505076A (ja) * 2017-02-01 2020-02-20 フェニックスソングス バイオロジカルズ，インコーポレイテッド 肝細胞の共培養を含む組成物および方法
WO2019060790A1 (en) * 2017-09-22 2019-03-28 Lifenet Health PLATE-BASED HEPATOCYTES AND PREPARATION AND USES THEREOF
EP3865572B1 (en) * 2018-10-11 2025-01-01 Ichimaru Pharcos Co., Ltd. Medium for growth of neuronal stem cells

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6069005A (en) 1991-08-07 2000-05-30 Albert Einstein College Of Medicine Of Yeshwa University Hapatoblasts and method of isolating same
WO1993003142A1 (en) * 1991-08-07 1993-02-18 Albert Einstein College Of Medicine Proliferation of hepatocyte precursors
ES2569778T3 (es) 1999-01-19 2016-05-12 The University Of North Carolina At Chapel Hill Células progenitoras hepáticas humanas
US7456017B2 (en) * 1999-10-01 2008-11-25 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
CN1742082A (zh) * 2002-03-15 2006-03-01 北卡罗来纳大学查伯山分校 原肝干细胞和近肝干细胞
CA2630355A1 (en) * 2005-11-16 2007-05-24 University Of North Carolina At Chapel Hill Extracellular matrix components for expansion or differentiation of hepatic progenitors
CA2689241A1 (en) * 2006-06-28 2008-01-03 Vesta Therapeutics, Inc. Matrix and method for isolation of hepatic progenitor cells
US20080248570A1 (en) * 2007-03-06 2008-10-09 University Of North Carolina At Chapel Hill Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of cells

Also Published As

Publication number Publication date
IL202738A0 (en) 2011-08-01
EP2171040A4 (en) 2010-08-04
JP2010529855A (ja) 2010-09-02
KR20100094970A (ko) 2010-08-27
AU2008266992A1 (en) 2008-12-24
WO2008156667A1 (en) 2008-12-24
CN101868533A (zh) 2010-10-20
MX2009013819A (es) 2010-05-17
US8404483B2 (en) 2013-03-26
BRPI0813353A2 (pt) 2014-10-14
EP2171040A1 (en) 2010-04-07
IN2010KN00157A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-08-28
US20110065188A1 (en) 2011-03-17
US20080318316A1 (en) 2008-12-25
TW200916581A (en) 2009-04-16

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Effective date: 20150615