CA2708311A1 - Prediction de genotoxicite - Google Patents
Prediction de genotoxicite Download PDFInfo
- Publication number
- CA2708311A1 CA2708311A1 CA2708311A CA2708311A CA2708311A1 CA 2708311 A1 CA2708311 A1 CA 2708311A1 CA 2708311 A CA2708311 A CA 2708311A CA 2708311 A CA2708311 A CA 2708311A CA 2708311 A1 CA2708311 A1 CA 2708311A1
- Authority
- CA
- Canada
- Prior art keywords
- kinases
- compound
- seq
- genotoxicity
- inhibit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000001738 genotoxic effect Effects 0.000 title claims abstract description 35
- 231100000025 genetic toxicology Toxicity 0.000 title claims abstract description 33
- 108091000080 Phosphotransferase Proteins 0.000 claims abstract description 99
- 102000020233 phosphotransferase Human genes 0.000 claims abstract description 99
- 150000001875 compounds Chemical class 0.000 claims abstract description 89
- 238000012360 testing method Methods 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims description 48
- 230000005764 inhibitory process Effects 0.000 claims description 28
- 101000976899 Homo sapiens Mitogen-activated protein kinase 15 Proteins 0.000 claims description 24
- 102100023483 Mitogen-activated protein kinase 15 Human genes 0.000 claims description 24
- 230000000694 effects Effects 0.000 claims description 20
- 230000027455 binding Effects 0.000 claims description 18
- 108010024986 Cyclin-Dependent Kinase 2 Proteins 0.000 claims description 15
- 102100033234 Cyclin-dependent kinase 17 Human genes 0.000 claims description 15
- 102100036239 Cyclin-dependent kinase 2 Human genes 0.000 claims description 15
- 102100040862 Dual specificity protein kinase CLK1 Human genes 0.000 claims description 15
- 102100036109 Dual specificity protein kinase TTK Human genes 0.000 claims description 15
- 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 claims description 15
- 102100022975 Glycogen synthase kinase-3 alpha Human genes 0.000 claims description 15
- 102100038104 Glycogen synthase kinase-3 beta Human genes 0.000 claims description 15
- 101000944358 Homo sapiens Cyclin-dependent kinase 17 Proteins 0.000 claims description 15
- 101000749294 Homo sapiens Dual specificity protein kinase CLK1 Proteins 0.000 claims description 15
- 101000659223 Homo sapiens Dual specificity protein kinase TTK Proteins 0.000 claims description 15
- 101000903717 Homo sapiens Glycogen synthase kinase-3 alpha Proteins 0.000 claims description 15
- 101000628578 Homo sapiens Serine/threonine-protein kinase 16 Proteins 0.000 claims description 15
- -1 KIT Proteins 0.000 claims description 15
- 102100026758 Serine/threonine-protein kinase 16 Human genes 0.000 claims description 15
- 102100033245 Cyclin-dependent kinase 16 Human genes 0.000 claims description 14
- 101000944357 Homo sapiens Cyclin-dependent kinase 16 Proteins 0.000 claims description 14
- 102100033144 Cyclin-dependent kinase 18 Human genes 0.000 claims description 10
- 102100026810 Cyclin-dependent kinase 7 Human genes 0.000 claims description 10
- 102100040858 Dual specificity protein kinase CLK4 Human genes 0.000 claims description 10
- 101000944341 Homo sapiens Cyclin-dependent kinase 18 Proteins 0.000 claims description 10
- 101000911952 Homo sapiens Cyclin-dependent kinase 7 Proteins 0.000 claims description 10
- 101000749298 Homo sapiens Dual specificity protein kinase CLK4 Proteins 0.000 claims description 10
- 102100040844 Dual specificity protein kinase CLK2 Human genes 0.000 claims description 8
- 101000749291 Homo sapiens Dual specificity protein kinase CLK2 Proteins 0.000 claims description 8
- 101000926535 Homo sapiens Interferon-induced, double-stranded RNA-activated protein kinase Proteins 0.000 claims description 8
- 101000950710 Homo sapiens Mitogen-activated protein kinase 6 Proteins 0.000 claims description 8
- 102100034170 Interferon-induced, double-stranded RNA-activated protein kinase Human genes 0.000 claims description 8
- 102100037801 Mitogen-activated protein kinase 6 Human genes 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 7
- 102100037916 Cyclin-dependent kinase 11B Human genes 0.000 claims description 6
- 102100040856 Dual specificity protein kinase CLK3 Human genes 0.000 claims description 6
- 101000749304 Homo sapiens Dual specificity protein kinase CLK3 Proteins 0.000 claims description 6
- 102100035044 Myosin light chain kinase, smooth muscle Human genes 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 5
- 102100038079 AP2-associated protein kinase 1 Human genes 0.000 claims description 3
- 102000004000 Aurora Kinase A Human genes 0.000 claims description 3
- 108090000461 Aurora Kinase A Proteins 0.000 claims description 3
- 102100032306 Aurora kinase B Human genes 0.000 claims description 3
- 102100026630 Aurora kinase C Human genes 0.000 claims description 3
- 102100024507 BMP-2-inducible protein kinase Human genes 0.000 claims description 3
- 102100033093 Calcium/calmodulin-dependent protein kinase type II subunit alpha Human genes 0.000 claims description 3
- 102100040751 Casein kinase II subunit alpha Human genes 0.000 claims description 3
- 108010025454 Cyclin-Dependent Kinase 5 Proteins 0.000 claims description 3
- 102100038113 Cyclin-dependent kinase 14 Human genes 0.000 claims description 3
- 102100036329 Cyclin-dependent kinase 3 Human genes 0.000 claims description 3
- 102100024456 Cyclin-dependent kinase 8 Human genes 0.000 claims description 3
- 102100024457 Cyclin-dependent kinase 9 Human genes 0.000 claims description 3
- 102100026805 Cyclin-dependent-like kinase 5 Human genes 0.000 claims description 3
- 102100038606 Death-associated protein kinase 3 Human genes 0.000 claims description 3
- 101000876610 Dictyostelium discoideum Extracellular signal-regulated kinase 2 Proteins 0.000 claims description 3
- 102100023275 Dual specificity mitogen-activated protein kinase kinase 3 Human genes 0.000 claims description 3
- 102100023274 Dual specificity mitogen-activated protein kinase kinase 4 Human genes 0.000 claims description 3
- 102100023401 Dual specificity mitogen-activated protein kinase kinase 6 Human genes 0.000 claims description 3
- 101000742699 Homo sapiens AP2-associated protein kinase 1 Proteins 0.000 claims description 3
- 101000798306 Homo sapiens Aurora kinase B Proteins 0.000 claims description 3
- 101000765862 Homo sapiens Aurora kinase C Proteins 0.000 claims description 3
- 101000944249 Homo sapiens Calcium/calmodulin-dependent protein kinase type II subunit alpha Proteins 0.000 claims description 3
- 101000892026 Homo sapiens Casein kinase II subunit alpha Proteins 0.000 claims description 3
- 101000892015 Homo sapiens Casein kinase II subunit alpha' Proteins 0.000 claims description 3
- 101000738400 Homo sapiens Cyclin-dependent kinase 11B Proteins 0.000 claims description 3
- 101000884374 Homo sapiens Cyclin-dependent kinase 14 Proteins 0.000 claims description 3
- 101000944345 Homo sapiens Cyclin-dependent kinase 19 Proteins 0.000 claims description 3
- 101000715946 Homo sapiens Cyclin-dependent kinase 3 Proteins 0.000 claims description 3
- 101000980937 Homo sapiens Cyclin-dependent kinase 8 Proteins 0.000 claims description 3
- 101000980930 Homo sapiens Cyclin-dependent kinase 9 Proteins 0.000 claims description 3
- 101000956149 Homo sapiens Death-associated protein kinase 3 Proteins 0.000 claims description 3
- 101001115394 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 3 Proteins 0.000 claims description 3
- 101001115395 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 4 Proteins 0.000 claims description 3
- 101000624426 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 6 Proteins 0.000 claims description 3
- 101001005128 Homo sapiens LIM domain kinase 1 Proteins 0.000 claims description 3
- 101001064870 Homo sapiens Lon protease homolog, mitochondrial Proteins 0.000 claims description 3
- 101001018978 Homo sapiens MAP kinase-interacting serine/threonine-protein kinase 2 Proteins 0.000 claims description 3
- 101001052493 Homo sapiens Mitogen-activated protein kinase 1 Proteins 0.000 claims description 3
- 101001052477 Homo sapiens Mitogen-activated protein kinase 4 Proteins 0.000 claims description 3
- 101000950687 Homo sapiens Mitogen-activated protein kinase 7 Proteins 0.000 claims description 3
- 101001022780 Homo sapiens Myosin light chain kinase, smooth muscle Proteins 0.000 claims description 3
- 101001001527 Homo sapiens Phosphatidylinositol 5-phosphate 4-kinase type-2 beta Proteins 0.000 claims description 3
- 101000692455 Homo sapiens Platelet-derived growth factor receptor beta Proteins 0.000 claims description 3
- 101000944909 Homo sapiens Ribosomal protein S6 kinase alpha-1 Proteins 0.000 claims description 3
- 101000661821 Homo sapiens Serine/threonine-protein kinase 17A Proteins 0.000 claims description 3
- 101000661819 Homo sapiens Serine/threonine-protein kinase 17B Proteins 0.000 claims description 3
- 101000628647 Homo sapiens Serine/threonine-protein kinase 24 Proteins 0.000 claims description 3
- 101000701393 Homo sapiens Serine/threonine-protein kinase 26 Proteins 0.000 claims description 3
- 101000880439 Homo sapiens Serine/threonine-protein kinase 3 Proteins 0.000 claims description 3
- 101001026870 Homo sapiens Serine/threonine-protein kinase D1 Proteins 0.000 claims description 3
- 101001026885 Homo sapiens Serine/threonine-protein kinase D3 Proteins 0.000 claims description 3
- 101000885383 Homo sapiens Serine/threonine-protein kinase DCLK3 Proteins 0.000 claims description 3
- 101000691614 Homo sapiens Serine/threonine-protein kinase PLK3 Proteins 0.000 claims description 3
- 101000582914 Homo sapiens Serine/threonine-protein kinase PLK4 Proteins 0.000 claims description 3
- 101000662993 Homo sapiens Serine/threonine-protein kinase TNNI3K Proteins 0.000 claims description 3
- 101000595531 Homo sapiens Serine/threonine-protein kinase pim-1 Proteins 0.000 claims description 3
- 101001001648 Homo sapiens Serine/threonine-protein kinase pim-2 Proteins 0.000 claims description 3
- 101001001645 Homo sapiens Serine/threonine-protein kinase pim-3 Proteins 0.000 claims description 3
- 102100026023 LIM domain kinase 1 Human genes 0.000 claims description 3
- 102100033610 MAP kinase-interacting serine/threonine-protein kinase 2 Human genes 0.000 claims description 3
- 102100024299 Maternal embryonic leucine zipper kinase Human genes 0.000 claims description 3
- 101710154611 Maternal embryonic leucine zipper kinase Proteins 0.000 claims description 3
- 102100024193 Mitogen-activated protein kinase 1 Human genes 0.000 claims description 3
- 102100024189 Mitogen-activated protein kinase 4 Human genes 0.000 claims description 3
- 102100037805 Mitogen-activated protein kinase 7 Human genes 0.000 claims description 3
- 102100030782 Myosin light chain kinase family member 4 Human genes 0.000 claims description 3
- 101710087570 Myosin light chain kinase family member 4 Proteins 0.000 claims description 3
- 101710198035 Myosin light chain kinase, smooth muscle Proteins 0.000 claims description 3
- 102100036137 Phosphatidylinositol 5-phosphate 4-kinase type-2 beta Human genes 0.000 claims description 3
- 102100026547 Platelet-derived growth factor receptor beta Human genes 0.000 claims description 3
- 102100033536 Ribosomal protein S6 kinase alpha-1 Human genes 0.000 claims description 3
- 102100037955 Serine/threonine-protein kinase 17A Human genes 0.000 claims description 3
- 102100037959 Serine/threonine-protein kinase 17B Human genes 0.000 claims description 3
- 102100026764 Serine/threonine-protein kinase 24 Human genes 0.000 claims description 3
- 102100030617 Serine/threonine-protein kinase 26 Human genes 0.000 claims description 3
- 102100037310 Serine/threonine-protein kinase D1 Human genes 0.000 claims description 3
- 102100037311 Serine/threonine-protein kinase D3 Human genes 0.000 claims description 3
- 102100039774 Serine/threonine-protein kinase DCLK3 Human genes 0.000 claims description 3
- 102100031463 Serine/threonine-protein kinase PLK1 Human genes 0.000 claims description 3
- 102100026209 Serine/threonine-protein kinase PLK3 Human genes 0.000 claims description 3
- 102100030267 Serine/threonine-protein kinase PLK4 Human genes 0.000 claims description 3
- 102100037670 Serine/threonine-protein kinase TNNI3K Human genes 0.000 claims description 3
- 102100036077 Serine/threonine-protein kinase pim-1 Human genes 0.000 claims description 3
- 102100036120 Serine/threonine-protein kinase pim-2 Human genes 0.000 claims description 3
- 102100036119 Serine/threonine-protein kinase pim-3 Human genes 0.000 claims description 3
- 102100033455 TGF-beta receptor type-2 Human genes 0.000 claims description 3
- 108010082684 Transforming Growth Factor-beta Type II Receptor Proteins 0.000 claims description 3
- 231100000734 genotoxic potential Toxicity 0.000 claims description 3
- 108010056274 polo-like kinase 1 Proteins 0.000 claims description 3
- 102100033363 Dual specificity tyrosine-phosphorylation-regulated kinase 1B Human genes 0.000 claims 6
- 101000926738 Homo sapiens Dual specificity tyrosine-phosphorylation-regulated kinase 1B Proteins 0.000 claims 6
- 239000007787 solid Substances 0.000 claims 3
- 101100218937 Homo sapiens BMP2K gene Proteins 0.000 claims 2
- 101000709250 Homo sapiens Serine/threonine-protein kinase SIK2 Proteins 0.000 claims 2
- 101000851018 Homo sapiens Vascular endothelial growth factor receptor 1 Proteins 0.000 claims 2
- 102100034377 Serine/threonine-protein kinase SIK2 Human genes 0.000 claims 2
- 102100033178 Vascular endothelial growth factor receptor 1 Human genes 0.000 claims 2
- 102100034356 Casein kinase I isoform alpha-like Human genes 0.000 claims 1
- 102100037402 Casein kinase I isoform delta Human genes 0.000 claims 1
- 101000994694 Homo sapiens Casein kinase I isoform alpha-like Proteins 0.000 claims 1
- 101001026336 Homo sapiens Casein kinase I isoform delta Proteins 0.000 claims 1
- 238000003556 assay Methods 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 10
- 238000012549 training Methods 0.000 description 9
- 102000001253 Protein Kinase Human genes 0.000 description 8
- 108060006633 protein kinase Proteins 0.000 description 8
- 238000002790 cross-validation Methods 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 6
- 238000003909 pattern recognition Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 102100023482 Mitogen-activated protein kinase 14 Human genes 0.000 description 3
- 102000001708 Protein Isoforms Human genes 0.000 description 3
- 108010029485 Protein Isoforms Proteins 0.000 description 3
- 239000003460 aneugen Substances 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 239000003280 clastogen Substances 0.000 description 3
- 231100000506 clastogen Toxicity 0.000 description 3
- 230000002596 correlated effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 231100000024 genotoxic Toxicity 0.000 description 3
- 108010068338 p38 Mitogen-Activated Protein Kinases Proteins 0.000 description 3
- 238000010187 selection method Methods 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 231100000244 chromosomal damage Toxicity 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000013537 high throughput screening Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229940043355 kinase inhibitor Drugs 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 230000011278 mitosis Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000012567 pattern recognition method Methods 0.000 description 2
- 238000011170 pharmaceutical development Methods 0.000 description 2
- 239000003757 phosphotransferase inhibitor Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 101001043761 Homo sapiens Inhibitor of nuclear factor kappa-B kinase subunit epsilon Proteins 0.000 description 1
- 102100021857 Inhibitor of nuclear factor kappa-B kinase subunit epsilon Human genes 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 235000006679 Mentha X verticillata Nutrition 0.000 description 1
- 235000002899 Mentha suaveolens Nutrition 0.000 description 1
- 235000001636 Mentha x rotundifolia Nutrition 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 231100000230 acceptable toxicity Toxicity 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 230000002603 aneugenic effect Effects 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000006287 biotinylation Effects 0.000 description 1
- 238000007413 biotinylation Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 231100000005 chromosome aberration Toxicity 0.000 description 1
- 231100000505 clastogenic Toxicity 0.000 description 1
- 230000003541 clastogenic effect Effects 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 230000007674 genetic toxicity Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002611 lead compounds Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 230000000865 phosphorylative effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007637 random forest analysis Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000012706 support-vector machine Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 231100000563 toxic property Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
- G01N33/5017—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity for testing neoplastic activity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Urology & Nephrology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1529107P | 2007-12-20 | 2007-12-20 | |
| US61/015,291 | 2007-12-20 | ||
| PCT/EP2008/010530 WO2009080219A1 (fr) | 2007-12-20 | 2008-12-11 | Prédiction de génotoxicité |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2708311A1 true CA2708311A1 (fr) | 2009-07-02 |
Family
ID=40386475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2708311A Abandoned CA2708311A1 (fr) | 2007-12-20 | 2008-12-11 | Prediction de genotoxicite |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP2225558A1 (fr) |
| JP (1) | JP2011505847A (fr) |
| CN (1) | CN101903774A (fr) |
| CA (1) | CA2708311A1 (fr) |
| WO (1) | WO2009080219A1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2861985B1 (fr) * | 2012-06-13 | 2017-11-15 | Merck Patent GmbH | Analyses de l'expression de protéines pour l'identification de composés génotoxiques |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008518598A (ja) * | 2004-10-29 | 2008-06-05 | ノバルティス アクチエンゲゼルシャフト | 薬剤の毒性評価 |
| WO2007084968A1 (fr) * | 2006-01-18 | 2007-07-26 | Invitrogen Corporation | Procédés de mesure de l'activité d'une kinase |
-
2008
- 2008-12-11 JP JP2010538419A patent/JP2011505847A/ja active Pending
- 2008-12-11 CN CN2008801205410A patent/CN101903774A/zh active Pending
- 2008-12-11 EP EP08864122A patent/EP2225558A1/fr not_active Withdrawn
- 2008-12-11 CA CA2708311A patent/CA2708311A1/fr not_active Abandoned
- 2008-12-11 WO PCT/EP2008/010530 patent/WO2009080219A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| EP2225558A1 (fr) | 2010-09-08 |
| CN101903774A (zh) | 2010-12-01 |
| JP2011505847A (ja) | 2011-03-03 |
| WO2009080219A1 (fr) | 2009-07-02 |
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