CA2707944A1 - Procedes de generation de plantes transgeniques sans marqueur - Google Patents
Procedes de generation de plantes transgeniques sans marqueur Download PDFInfo
- Publication number
- CA2707944A1 CA2707944A1 CA2707944A CA2707944A CA2707944A1 CA 2707944 A1 CA2707944 A1 CA 2707944A1 CA 2707944 A CA2707944 A CA 2707944A CA 2707944 A CA2707944 A CA 2707944A CA 2707944 A1 CA2707944 A1 CA 2707944A1
- Authority
- CA
- Canada
- Prior art keywords
- vire2
- gene
- strain
- dna
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000003550 marker Substances 0.000 title claims description 84
- 238000000034 method Methods 0.000 title claims description 47
- 230000009261 transgenic effect Effects 0.000 title claims description 15
- 241000589158 Agrobacterium Species 0.000 claims abstract description 85
- 108090000623 proteins and genes Proteins 0.000 claims description 226
- 101150102050 virE2 gene Proteins 0.000 claims description 215
- 239000013612 plasmid Substances 0.000 claims description 143
- 239000013598 vector Substances 0.000 claims description 26
- 238000003780 insertion Methods 0.000 claims description 19
- 230000037431 insertion Effects 0.000 claims description 19
- 238000012217 deletion Methods 0.000 claims description 8
- 230000037430 deletion Effects 0.000 claims description 8
- 230000009466 transformation Effects 0.000 abstract description 38
- 238000011426 transformation method Methods 0.000 abstract description 10
- 241000196324 Embryophyta Species 0.000 description 169
- 210000004027 cell Anatomy 0.000 description 88
- 102000004169 proteins and genes Human genes 0.000 description 54
- 108020004414 DNA Proteins 0.000 description 39
- 108091028043 Nucleic acid sequence Proteins 0.000 description 27
- 150000007523 nucleic acids Chemical class 0.000 description 27
- 238000013518 transcription Methods 0.000 description 25
- 230000035897 transcription Effects 0.000 description 25
- 230000001105 regulatory effect Effects 0.000 description 16
- 238000011282 treatment Methods 0.000 description 16
- 108700026244 Open Reading Frames Proteins 0.000 description 14
- 210000001519 tissue Anatomy 0.000 description 14
- 229920003266 Leaf® Polymers 0.000 description 13
- 241000894007 species Species 0.000 description 13
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 12
- 240000003768 Solanum lycopersicum Species 0.000 description 12
- 102000039446 nucleic acids Human genes 0.000 description 12
- 108020004707 nucleic acids Proteins 0.000 description 12
- 101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 11
- 238000012546 transfer Methods 0.000 description 11
- 241000208125 Nicotiana Species 0.000 description 10
- 230000002068 genetic effect Effects 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
- 108091026890 Coding region Proteins 0.000 description 9
- 101100484795 Hypocrea virens (strain Gv29-8 / FGSC 10586) virE gene Proteins 0.000 description 9
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 9
- 230000006870 function Effects 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 8
- 239000004009 herbicide Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 101150033532 virG gene Proteins 0.000 description 8
- 101100018379 Shigella flexneri icsA gene Proteins 0.000 description 7
- 101100476911 Yersinia enterocolitica yscW gene Proteins 0.000 description 7
- 239000002299 complementary DNA Substances 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 230000002363 herbicidal effect Effects 0.000 description 7
- 230000001939 inductive effect Effects 0.000 description 7
- 230000010354 integration Effects 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 108090000765 processed proteins & peptides Proteins 0.000 description 7
- 230000001018 virulence Effects 0.000 description 7
- 240000001980 Cucurbita pepo Species 0.000 description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
- 230000006801 homologous recombination Effects 0.000 description 6
- 238000002744 homologous recombination Methods 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- HULCBRRKIWCSOF-UHNVWZDZSA-N (2r)-2-[[(1s)-3-amino-1-carboxy-3-oxopropyl]amino]pentanedioic acid Chemical compound NC(=O)C[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O HULCBRRKIWCSOF-UHNVWZDZSA-N 0.000 description 5
- 235000009852 Cucurbita pepo Nutrition 0.000 description 5
- HULCBRRKIWCSOF-UHFFFAOYSA-N L,L-succinamopine Natural products NC(=O)CC(C(O)=O)NC(C(O)=O)CCC(O)=O HULCBRRKIWCSOF-UHFFFAOYSA-N 0.000 description 5
- 241000207763 Solanum Species 0.000 description 5
- 230000000692 anti-sense effect Effects 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 230000010076 replication Effects 0.000 description 5
- 238000000844 transformation Methods 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 230000030279 gene silencing Effects 0.000 description 4
- 235000003869 genetically modified organism Nutrition 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- 230000003362 replicative effect Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 3
- 208000003322 Coinfection Diseases 0.000 description 3
- 241000219112 Cucumis Species 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 3
- 241000227653 Lycopersicon Species 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 235000002634 Solanum Nutrition 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- 238000004422 calculation algorithm Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000012226 gene silencing method Methods 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000008488 polyadenylation Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 230000032258 transport Effects 0.000 description 3
- 108020005345 3' Untranslated Regions Proteins 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 235000000832 Ayote Nutrition 0.000 description 2
- 241000219198 Brassica Species 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 240000008574 Capsicum frutescens Species 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 244000193629 Cyphomandra crassifolia Species 0.000 description 2
- IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 description 2
- IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical compound [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 244000282866 Euchlaena mexicana Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 241000219146 Gossypium Species 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 244000020551 Helianthus annuus Species 0.000 description 2
- 235000003222 Helianthus annuus Nutrition 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 241000208822 Lactuca Species 0.000 description 2
- 235000002262 Lycopersicon Nutrition 0.000 description 2
- 240000004658 Medicago sativa Species 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000209046 Pennisetum Species 0.000 description 2
- 108010033276 Peptide Fragments Proteins 0.000 description 2
- 102000007079 Peptide Fragments Human genes 0.000 description 2
- 241000219833 Phaseolus Species 0.000 description 2
- 235000010582 Pisum sativum Nutrition 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- 244000082988 Secale cereale Species 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 235000002597 Solanum melongena Nutrition 0.000 description 2
- 244000061458 Solanum melongena Species 0.000 description 2
- 240000007417 Solanum muricatum Species 0.000 description 2
- 240000002407 Solanum quitoense Species 0.000 description 2
- 244000070646 Solanum topiro Species 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 108700019146 Transgenes Proteins 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 244000083398 Zea diploperennis Species 0.000 description 2
- 241000746970 Zea luxurians Species 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 244000209671 Zea mays subsp parviglumis Species 0.000 description 2
- 241000172407 Zea mays subsp. huehuetenangensis Species 0.000 description 2
- 241000209127 Zea perennis Species 0.000 description 2
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000036952 cancer formation Effects 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 231100000504 carcinogenesis Toxicity 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 238000000126 in silico method Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 231100000590 oncogenic Toxicity 0.000 description 2
- 230000002246 oncogenic effect Effects 0.000 description 2
- 230000001915 proofreading effect Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 230000005030 transcription termination Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 101150084750 1 gene Proteins 0.000 description 1
- 101150072531 10 gene Proteins 0.000 description 1
- 101150098072 20 gene Proteins 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical compound NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 1
- 241000743339 Agrostis Species 0.000 description 1
- 241000207875 Antirrhinum Species 0.000 description 1
- 108020004491 Antisense DNA Proteins 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 241001106067 Atropa Species 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 244000178993 Brassica juncea Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 240000008100 Brassica rapa Species 0.000 description 1
- 241000209200 Bromus Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 235000008697 Cannabis sativa Nutrition 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 235000002567 Capsicum annuum Nutrition 0.000 description 1
- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 241000219109 Citrullus Species 0.000 description 1
- 235000009831 Citrullus lanatus Nutrition 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 244000270200 Citrullus vulgaris Species 0.000 description 1
- 235000012840 Citrullus vulgaris Nutrition 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000009842 Cucumis melo Nutrition 0.000 description 1
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000219122 Cucurbita Species 0.000 description 1
- 240000007092 Cucurbita argyrosperma Species 0.000 description 1
- 235000003949 Cucurbita mixta Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
- 235000000298 Cyphomandra crassifolia Nutrition 0.000 description 1
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 241000208296 Datura Species 0.000 description 1
- 241000208175 Daucus Species 0.000 description 1
- 240000001879 Digitalis lutea Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 244000166124 Eucalyptus globulus Species 0.000 description 1
- 235000007039 Euchlaena mexicana Nutrition 0.000 description 1
- 108091029865 Exogenous DNA Proteins 0.000 description 1
- 241000234642 Festuca Species 0.000 description 1
- 241000220223 Fragaria Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000204888 Geobacter sp. Species 0.000 description 1
- 241000208152 Geranium Species 0.000 description 1
- 241000735332 Gerbera Species 0.000 description 1
- 241001139242 Gloeotrichia pisum Species 0.000 description 1
- 235000009438 Gossypium Nutrition 0.000 description 1
- 240000000047 Gossypium barbadense Species 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000208278 Hyoscyamus Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 241000758789 Juglans Species 0.000 description 1
- 235000013757 Juglans Nutrition 0.000 description 1
- 235000003167 Lactuca indica Nutrition 0.000 description 1
- 235000007661 Lactuca perennis Nutrition 0.000 description 1
- 240000004628 Lactuca perennis Species 0.000 description 1
- 240000005993 Lactuca saligna Species 0.000 description 1
- 235000003126 Lactuca saligna Nutrition 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000208204 Linum Species 0.000 description 1
- 241000665629 Linum flavum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 241000121629 Majorana Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 241000219823 Medicago Species 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 241001282315 Nemesis Species 0.000 description 1
- 241000207746 Nicotiana benthamiana Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091005461 Nucleic proteins Chemical group 0.000 description 1
- 241000219830 Onobrychis Species 0.000 description 1
- 241000209094 Oryza Species 0.000 description 1
- 240000002582 Oryza sativa Indica Group Species 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 241000208181 Pelargonium Species 0.000 description 1
- 244000038248 Pennisetum spicatum Species 0.000 description 1
- 244000115721 Pennisetum typhoides Species 0.000 description 1
- 235000007195 Pennisetum typhoides Nutrition 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 241000219843 Pisum Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 108091036407 Polyadenylation Proteins 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 240000001536 Prunus fruticosa Species 0.000 description 1
- 240000005809 Prunus persica Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 108091030071 RNAI Proteins 0.000 description 1
- 241000218206 Ranunculus Species 0.000 description 1
- 241000220259 Raphanus Species 0.000 description 1
- 108010034634 Repressor Proteins Proteins 0.000 description 1
- 108700025701 Retinoblastoma Genes Proteins 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 241001106018 Salpiglossis Species 0.000 description 1
- 241000780602 Senecio Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 235000008515 Setaria glauca Nutrition 0.000 description 1
- 241000220261 Sinapis Species 0.000 description 1
- 108091027967 Small hairpin RNA Proteins 0.000 description 1
- 241001291279 Solanum galapagense Species 0.000 description 1
- 235000018246 Solanum hyporhodium Nutrition 0.000 description 1
- 235000002560 Solanum lycopersicum Nutrition 0.000 description 1
- 235000003953 Solanum lycopersicum var cerasiforme Nutrition 0.000 description 1
- 235000018252 Solanum quitoense Nutrition 0.000 description 1
- 235000018256 Solanum topiro Nutrition 0.000 description 1
- 235000007230 Sorghum bicolor Nutrition 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 241001312519 Trigonella Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 108010059993 Vancomycin Proteins 0.000 description 1
- 244000071378 Viburnum opulus Species 0.000 description 1
- 241000219977 Vigna Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 235000007241 Zea diploperennis Nutrition 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000017556 Zea mays subsp parviglumis Nutrition 0.000 description 1
- 241000482268 Zea mays subsp. mays Species 0.000 description 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 108091006088 activator proteins Proteins 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 244000193174 agave Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000003816 antisense DNA Substances 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000004790 biotic stress Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 239000001511 capsicum annuum Substances 0.000 description 1
- 229960004261 cefotaxime Drugs 0.000 description 1
- GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- -1 eGFP Substances 0.000 description 1
- 230000005014 ectopic expression Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000009368 gene silencing by RNA Effects 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 210000005061 intracellular organelle Anatomy 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 235000008779 pepino Nutrition 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 235000021018 plums Nutrition 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 230000003584 silencer Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000020354 squash Nutrition 0.000 description 1
- 239000010421 standard material Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 108091005703 transmembrane proteins Proteins 0.000 description 1
- 102000035160 transmembrane proteins Human genes 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07122354.9 | 2007-12-05 | ||
| EP07122354 | 2007-12-05 | ||
| PCT/NL2008/050775 WO2009072883A1 (fr) | 2007-12-05 | 2008-12-05 | Procédés de génération de plantes transgéniques sans marqueur |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2707944A1 true CA2707944A1 (fr) | 2009-06-11 |
Family
ID=39125235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2707944A Abandoned CA2707944A1 (fr) | 2007-12-05 | 2008-12-05 | Procedes de generation de plantes transgeniques sans marqueur |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP2217616A1 (fr) |
| JP (1) | JP2011505806A (fr) |
| CN (1) | CN101983206A (fr) |
| AU (1) | AU2008332063A1 (fr) |
| BR (1) | BRPI0820100A2 (fr) |
| CA (1) | CA2707944A1 (fr) |
| IL (1) | IL206177A0 (fr) |
| NZ (1) | NZ585926A (fr) |
| WO (1) | WO2009072883A1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753813B (zh) * | 2018-06-08 | 2021-08-24 | 中国水稻研究所 | 获得无标记转基因植物的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1117816B1 (fr) * | 1998-10-01 | 2005-12-21 | Pioneer Hi-Bred International, Inc. | Procede de transformation de vegetaux |
-
2008
- 2008-12-05 AU AU2008332063A patent/AU2008332063A1/en not_active Abandoned
- 2008-12-05 NZ NZ585926A patent/NZ585926A/en not_active IP Right Cessation
- 2008-12-05 CN CN2008801262108A patent/CN101983206A/zh active Pending
- 2008-12-05 WO PCT/NL2008/050775 patent/WO2009072883A1/fr active Application Filing
- 2008-12-05 EP EP08856375A patent/EP2217616A1/fr not_active Withdrawn
- 2008-12-05 JP JP2010536869A patent/JP2011505806A/ja not_active Withdrawn
- 2008-12-05 CA CA2707944A patent/CA2707944A1/fr not_active Abandoned
- 2008-12-05 BR BRPI0820100-5A patent/BRPI0820100A2/pt not_active IP Right Cessation
-
2010
- 2010-06-03 IL IL206177A patent/IL206177A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009072883A1 (fr) | 2009-06-11 |
| NZ585926A (en) | 2012-02-24 |
| IL206177A0 (en) | 2010-12-30 |
| EP2217616A1 (fr) | 2010-08-18 |
| JP2011505806A (ja) | 2011-03-03 |
| BRPI0820100A2 (pt) | 2015-06-16 |
| CN101983206A (zh) | 2011-03-02 |
| AU2008332063A1 (en) | 2009-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6530887B2 (ja) | 植物形質転換率を高めるように改変されたアグロバクテリウム（Ａｇｒｏｂａｃｔｅｒｉｕｍ）株 | |
| JP6871260B2 (ja) | 改良された植物形質転換の方法および組成物 | |
| AU2006325585A1 (en) | Constitutive plant promoters | |
| US20230130592A1 (en) | Novel agrobacterium tumefaciens strains | |
| AU2010257316B2 (en) | Transformation Vectors | |
| US20240002870A1 (en) | Rapid transformation of monocot leaf explants | |
| US20120042409A1 (en) | Plant transformation using dna minicircles | |
| US20170240911A1 (en) | Agrobacterium-mediated site specific integration | |
| CA2535866A1 (fr) | Procedes et compositions accroissant la transformation de cellules vegetales | |
| WO2018082611A1 (fr) | Construction d'acide nucléique exprimant un gène exogène dans des cellules végétales et utilisation associée | |
| US7659447B2 (en) | Increasing host plant susceptibility to agrobacterium infection by overexpression of the arabidopsis VIP1 gene | |
| US20100257632A1 (en) | Methods for generating marker-free transgenic plants | |
| CA2707944A1 (fr) | Procedes de generation de plantes transgeniques sans marqueur | |
| WO2020171192A1 (fr) | Acide nucléique pour l'édition du génome d'une cellule végétale et son utilisation | |
| JP4595631B2 (ja) | 選抜マーカー遺伝子の影響が排除された遺伝子導入細胞、組織又は植物の作成方法 | |
| JP7518545B2 (ja) | 植物細胞のゲノム編集用核酸及びその用途 | |
| EP1630233B1 (fr) | Nouveau vecteur | |
| Ratnayaka | Agrobacterium mediated gene transformation of rice: Comparison of callus and shoot apex derived plants | |
| JP5114161B2 (ja) | 新規な部位特異的組換え酵素認識配列及びベクター | |
| JP2005192551A (ja) | 新規ベクター | |
| NZ574191A (en) | Plant transformation using DNA minicircles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20141205 |