CA2630658C - Method of culturing unicellular organisms - Google Patents
Method of culturing unicellular organisms Download PDFInfo
- Publication number
- CA2630658C CA2630658C CA2630658A CA2630658A CA2630658C CA 2630658 C CA2630658 C CA 2630658C CA 2630658 A CA2630658 A CA 2630658A CA 2630658 A CA2630658 A CA 2630658A CA 2630658 C CA2630658 C CA 2630658C
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- CA
- Canada
- Prior art keywords
- sample
- reservoir
- pressure
- culture
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000034 method Methods 0.000 title claims abstract description 38
- 238000012258 culturing Methods 0.000 title description 7
- 244000005700 microbiome Species 0.000 claims abstract description 43
- 239000012530 fluid Substances 0.000 claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 239000004215 Carbon black (E152) Substances 0.000 claims description 21
- 229930195733 hydrocarbon Natural products 0.000 claims description 21
- 150000002430 hydrocarbons Chemical class 0.000 claims description 20
- 238000011534 incubation Methods 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 239000011435 rock Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 5
- 230000002708 enhancing effect Effects 0.000 claims description 2
- 125000001183 hydrocarbyl group Chemical group 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 20
- 241000894006 Bacteria Species 0.000 description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000005755 formation reaction Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229910000831 Steel Inorganic materials 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 239000002480 mineral oil Substances 0.000 description 3
- 235000010446 mineral oil Nutrition 0.000 description 3
- 239000010959 steel Substances 0.000 description 3
- 241000203069 Archaea Species 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000196835 Achaea Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229920002449 FKM Polymers 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- WAIPAZQMEIHHTJ-UHFFFAOYSA-N [Cr].[Co] Chemical compound [Cr].[Co] WAIPAZQMEIHHTJ-UHFFFAOYSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 238000003935 denaturing gradient gel electrophoresis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000002455 scale inhibitor Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/26—Processes using, or culture media containing, hydrocarbons
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Sustainable Development (AREA)
- Mechanical Engineering (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0524193A GB2432587A (en) | 2005-11-28 | 2005-11-28 | Method of culturing microorganisms from subterranean wells |
| GB0524193.0 | 2005-11-28 | ||
| PCT/GB2006/004443 WO2007060473A1 (en) | 2005-11-28 | 2006-11-28 | Method of culturing unicellular organisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2630658A1 CA2630658A1 (en) | 2007-05-31 |
| CA2630658C true CA2630658C (en) | 2016-03-15 |
Family
ID=35601354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2630658A Active CA2630658C (en) | 2005-11-28 | 2006-11-28 | Method of culturing unicellular organisms |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20090069201A1 (ja) |
| EP (1) | EP1960509B1 (ja) |
| JP (1) | JP2009517068A (ja) |
| CN (1) | CN101336289B (ja) |
| CA (1) | CA2630658C (ja) |
| GB (1) | GB2432587A (ja) |
| WO (1) | WO2007060473A1 (ja) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8826976B2 (en) | 2011-02-08 | 2014-09-09 | Wintershall Holding GmbH | Multistage process for producing mineral oil using microorganisms |
| CA2823750A1 (en) * | 2011-02-08 | 2012-08-16 | Wintershall Holding GmbH | Multistage process for producing mineral oil using microorganisms |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3892130A (en) * | 1974-02-11 | 1975-07-01 | Us Navy | Deep sea microbiological sampling and culturing apparatus and method |
| JPS62244377A (ja) * | 1986-04-15 | 1987-10-24 | Agency Of Ind Science & Technol | 微生物を高圧状態で単離、培養する装置 |
| JPH078227B2 (ja) * | 1987-04-25 | 1995-02-01 | 株式会社丸菱バイオエンジ | 深海微生物培養装置 |
| US4971151A (en) * | 1988-04-19 | 1990-11-20 | B.W.N. Live-Oil Pty. Ltd. | Recovery of oil from oil reservoirs |
| US5297625A (en) * | 1990-08-24 | 1994-03-29 | Associated Universities, Inc. | Biochemically enhanced oil recovery and oil treatment |
| US5377755A (en) * | 1992-11-16 | 1995-01-03 | Western Atlas International, Inc. | Method and apparatus for acquiring and processing subsurface samples of connate fluid |
| JPH06213147A (ja) * | 1993-01-20 | 1994-08-02 | Shokuhin Sangyo Chokoatsu Riyou Gijutsu Kenkyu Kumiai | 液体の加圧処理方法及び装置 |
| US6543535B2 (en) * | 2000-03-15 | 2003-04-08 | Exxonmobil Upstream Research Company | Process for stimulating microbial activity in a hydrocarbon-bearing, subterranean formation |
| GB0112343D0 (en) * | 2001-05-21 | 2001-07-11 | Norske Stats Oljeselskap | Well treatment |
| GB0412059D0 (en) * | 2004-05-28 | 2004-06-30 | Univ Newcastle | Process for stimulating production of hydrogen from petroleum in subterranean formations |
-
2005
- 2005-11-28 GB GB0524193A patent/GB2432587A/en not_active Withdrawn
-
2006
- 2006-11-28 CA CA2630658A patent/CA2630658C/en active Active
- 2006-11-28 JP JP2008542824A patent/JP2009517068A/ja active Pending
- 2006-11-28 WO PCT/GB2006/004443 patent/WO2007060473A1/en active Application Filing
- 2006-11-28 EP EP06808702.2A patent/EP1960509B1/en active Active
- 2006-11-28 US US12/094,939 patent/US20090069201A1/en not_active Abandoned
- 2006-11-28 CN CN2006800518067A patent/CN101336289B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| GB0524193D0 (en) | 2006-01-04 |
| GB2432587A (en) | 2007-05-30 |
| CN101336289A (zh) | 2008-12-31 |
| EP1960509A1 (en) | 2008-08-27 |
| US20090069201A1 (en) | 2009-03-12 |
| WO2007060473A1 (en) | 2007-05-31 |
| JP2009517068A (ja) | 2009-04-30 |
| CA2630658A1 (en) | 2007-05-31 |
| CN101336289B (zh) | 2012-07-04 |
| EP1960509B1 (en) | 2018-10-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |