CA2611588A1 - Method for the segmentation of leukocytes - Google Patents
Method for the segmentation of leukocytes Download PDFInfo
- Publication number
- CA2611588A1 CA2611588A1 CA002611588A CA2611588A CA2611588A1 CA 2611588 A1 CA2611588 A1 CA 2611588A1 CA 002611588 A CA002611588 A CA 002611588A CA 2611588 A CA2611588 A CA 2611588A CA 2611588 A1 CA2611588 A1 CA 2611588A1
- Authority
- CA
- Canada
- Prior art keywords
- value
- colour
- pixels
- probability
- image
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 210000000265 leukocyte Anatomy 0.000 title abstract description 7
- 230000011218 segmentation Effects 0.000 title abstract description 4
- 210000004369 blood Anatomy 0.000 claims abstract description 9
- 239000008280 blood Substances 0.000 claims abstract description 9
- 230000009466 transformation Effects 0.000 claims abstract description 6
- 210000003743 erythrocyte Anatomy 0.000 claims description 7
- 230000006870 function Effects 0.000 claims description 7
- 238000003064 k means clustering Methods 0.000 claims description 5
- 210000000805 cytoplasm Anatomy 0.000 claims description 4
- 101100365516 Mus musculus Psat1 gene Proteins 0.000 claims description 3
- 238000004590 computer program Methods 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 235000019646 color tone Nutrition 0.000 abstract 3
- 210000004940 nucleus Anatomy 0.000 description 11
- 238000011156 evaluation Methods 0.000 description 4
- 238000004422 calculation algorithm Methods 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 210000003855 cell nucleus Anatomy 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 230000003628 erosive effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- YGLMVCVJLXREAK-MTVMDMGHSA-N 1,1-dimethyl-3-[(1S,2R,6R,7S,8R)-8-tricyclo[5.2.1.02,6]decanyl]urea Chemical compound C([C@H]12)CC[C@@H]1[C@@H]1C[C@@H](NC(=O)N(C)C)[C@H]2C1 YGLMVCVJLXREAK-MTVMDMGHSA-N 0.000 description 1
- 235000010716 Vigna mungo Nutrition 0.000 description 1
- 244000042295 Vigna mungo Species 0.000 description 1
- 238000013528 artificial neural network Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012854 evaluation process Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/11—Region-based segmentation
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/143—Segmentation; Edge detection involving probabilistic approaches, e.g. Markov random field [MRF] modelling
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/90—Determination of colour characteristics
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10056—Microscopic image
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10064—Fluorescence image
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
Landscapes
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Theoretical Computer Science (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Software Systems (AREA)
- Analytical Chemistry (AREA)
- Probability & Statistics with Applications (AREA)
- Molecular Biology (AREA)
- Signal Processing (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Multimedia (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Image Analysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Image Processing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ATA1010/2005 | 2005-06-15 | ||
| AT0101005A AT501944B1 (de) | 2005-06-15 | 2005-06-15 | Verfahren zum segmentieren von leukozyten |
| PCT/AT2006/000244 WO2006133473A1 (de) | 2005-06-15 | 2006-06-14 | Verfahren zum segmentieren von leukozyten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2611588A1 true CA2611588A1 (en) | 2006-12-21 |
Family
ID=36746668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002611588A Abandoned CA2611588A1 (en) | 2005-06-15 | 2006-06-14 | Method for the segmentation of leukocytes |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8064680B2 (pt-PT) |
| EP (1) | EP1891602B1 (pt-PT) |
| JP (1) | JP2008546990A (pt-PT) |
| CN (1) | CN101238487B (pt-PT) |
| AT (2) | AT501944B1 (pt-PT) |
| AU (1) | AU2006257752B2 (pt-PT) |
| CA (1) | CA2611588A1 (pt-PT) |
| WO (1) | WO2006133473A1 (pt-PT) |
| ZA (1) | ZA200710297B (pt-PT) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2374686T3 (es) * | 2007-05-14 | 2012-02-21 | Historx, Inc. | Separación en compartimentos por caracterización de píxel usando agrupamiento de datos de imágenes. |
| DE102008030874A1 (de) * | 2008-06-30 | 2010-01-07 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren und Vorrichtung zum Ermitteln einer Kontur und eines Mittelpunktes eines Objekts |
| WO2010009498A1 (en) * | 2008-07-21 | 2010-01-28 | Commonwealth Scientific And Industrial Research Organisation | Method and system for autonomous habitat analysis |
| CA2783935A1 (en) * | 2009-09-18 | 2011-03-24 | Andrew Janowczyk | High-throughput biomarker segmentation utilizing hierarchical normalized cuts |
| US8770954B2 (en) | 2010-02-10 | 2014-07-08 | KickSmart International, Inc. | Human-powered irrigation pump |
| KR101191454B1 (ko) | 2010-05-14 | 2012-10-16 | 계명대학교 산학협력단 | 비모수적 확률 모델과 질감 정보를 이용한 백혈구 분할 방법 |
| CN101923712A (zh) * | 2010-08-03 | 2010-12-22 | 苏州大学 | 基于粒子群优化的K-means聚类算法的基因芯片图像分割法 |
| US9522396B2 (en) | 2010-12-29 | 2016-12-20 | S.D. Sight Diagnostics Ltd. | Apparatus and method for automatic detection of pathogens |
| US20120207360A1 (en) * | 2011-02-11 | 2012-08-16 | Courosh Mehanian | Systems and Methods for Object Identification |
| CN104169719B (zh) | 2011-12-29 | 2017-03-08 | 思迪赛特诊断有限公司 | 用于检测生物样品中病原体的方法和系统 |
| CN103020639A (zh) * | 2012-11-27 | 2013-04-03 | 河海大学 | 一种白细胞自动识别计数方法 |
| JP5413501B1 (ja) * | 2012-12-07 | 2014-02-12 | 富士ゼロックス株式会社 | 画像処理装置、画像処理システム及びプログラム |
| WO2014188405A1 (en) | 2013-05-23 | 2014-11-27 | Parasight Ltd. | Method and system for imaging a cell sample |
| IL227276A0 (en) | 2013-07-01 | 2014-03-06 | Parasight Ltd | A method and system for obtaining a monolayer of cells, for use specifically for diagnosis |
| US10831013B2 (en) | 2013-08-26 | 2020-11-10 | S.D. Sight Diagnostics Ltd. | Digital microscopy systems, methods and computer program products |
| CN103489187A (zh) * | 2013-09-23 | 2014-01-01 | 华南理工大学 | 基于质量检测的宫颈lct图像中细胞核的分割方法 |
| CN103679184A (zh) * | 2013-12-06 | 2014-03-26 | 河海大学 | 一种基于相关向量机的白细胞自动识别方法 |
| WO2015142923A1 (en) * | 2014-03-17 | 2015-09-24 | Carnegie Mellon University | Methods and systems for disease classification |
| SE538435C2 (en) * | 2014-05-14 | 2016-06-28 | Cellavision Ab | Method, device and computer program product for determining color transforms between images comprising a plurality of image elements |
| US10482595B2 (en) | 2014-08-27 | 2019-11-19 | S.D. Sight Diagnostics Ltd. | System and method for calculating focus variation for a digital microscope |
| CN105205804B (zh) * | 2015-08-18 | 2018-10-30 | 爱威科技股份有限公司 | 血细胞图像中白细胞的核浆分离方法、分类方法及装置 |
| US10488644B2 (en) | 2015-09-17 | 2019-11-26 | S.D. Sight Diagnostics Ltd. | Methods and apparatus for detecting an entity in a bodily sample |
| EP3436864B1 (en) | 2016-03-30 | 2021-04-28 | S.D. Sight Diagnostics Ltd. | Image processing device for identifying blood parasites |
| BR112018072627A2 (pt) | 2016-05-11 | 2019-02-19 | S D Sight Diagnostics Ltd | realização de medições óticas em uma amostra |
| US11307196B2 (en) | 2016-05-11 | 2022-04-19 | S.D. Sight Diagnostics Ltd. | Sample carrier for optical measurements |
| US10430943B2 (en) * | 2016-10-07 | 2019-10-01 | Sony Corporation | Automated nuclei area/number estimation for IHC image analysis |
| CN111788471B (zh) | 2017-11-14 | 2023-12-12 | 思迪赛特诊断有限公司 | 用于光学测量的样品载体 |
| CN108805865B (zh) * | 2018-05-22 | 2019-12-10 | 杭州智微信息科技有限公司 | 一种基于饱和度聚类的骨髓白细胞定位方法 |
| CN109507406A (zh) * | 2018-11-30 | 2019-03-22 | 苏州深析智能科技有限公司 | 一种细胞形态智能提取方法、装置及分析方法 |
| CN109816629B (zh) * | 2018-12-20 | 2023-10-13 | 新绎健康科技有限公司 | 一种基于k-means聚类的苔质分离方法和装置 |
| CN111429461B (zh) * | 2019-01-09 | 2023-09-01 | 武汉兰丁智能医学股份有限公司 | 一种新型的重叠脱落上皮细胞分割方法 |
| CN110060229A (zh) * | 2019-05-29 | 2019-07-26 | 武汉兰丁医学高科技有限公司 | 一种骨髓白细胞的细胞自动定位分割方法 |
| KR102288332B1 (ko) * | 2019-10-16 | 2021-08-10 | (주)유아이엠디 | 골수 이미지 분석기를 이용한 골수세포 이미지 촬상 방법 |
| CN110766680B (zh) * | 2019-10-28 | 2021-06-22 | 闽江学院 | 一种基于几何约束的白细胞图像分割方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5352495A (en) * | 1976-10-25 | 1978-05-12 | Hitachi Ltd | Classification apparatus of leucocyte |
| JPS60211577A (ja) * | 1984-04-05 | 1985-10-23 | Hitachi Ltd | 白血球分類装置 |
| US6147198A (en) * | 1988-09-15 | 2000-11-14 | New York University | Methods and compositions for the manipulation and characterization of individual nucleic acid molecules |
| JPH02267675A (ja) * | 1989-04-10 | 1990-11-01 | Hitachi Ltd | 領域分割方法 |
| JPH04318460A (ja) * | 1991-04-17 | 1992-11-10 | Omron Corp | 血液細胞分析装置 |
| US6136540A (en) * | 1994-10-03 | 2000-10-24 | Ikonisys Inc. | Automated fluorescence in situ hybridization detection of genetic abnormalities |
| WO1999024465A1 (en) * | 1997-11-10 | 1999-05-20 | Dana-Farber Cancer Institute | Stabilized primate lentivirus envelope glycoproteins |
| IL132531A0 (en) * | 1999-10-22 | 2001-03-19 | Fbit Ltd | Detection and therapy of tumors |
| US7016787B2 (en) * | 2001-02-20 | 2006-03-21 | Cytokinetics, Inc. | Characterizing biological stimuli by response curves |
| US6832000B2 (en) * | 2001-03-28 | 2004-12-14 | Koninklijke Philips Electronics N.V. | Automatic segmentation-based grass detection for real-time video |
| JP2004340738A (ja) * | 2003-05-15 | 2004-12-02 | Sysmex Corp | 細胞分類装置とシステム及び細胞分類方法 |
| US20050014217A1 (en) * | 2003-07-18 | 2005-01-20 | Cytokinetics, Inc. | Predicting hepatotoxicity using cell based assays |
| US7323318B2 (en) * | 2004-07-15 | 2008-01-29 | Cytokinetics, Inc. | Assay for distinguishing live and dead cells |
-
2005
- 2005-06-15 AT AT0101005A patent/AT501944B1/de not_active IP Right Cessation
-
2006
- 2006-06-14 EP EP06741040A patent/EP1891602B1/de not_active Not-in-force
- 2006-06-14 CA CA002611588A patent/CA2611588A1/en not_active Abandoned
- 2006-06-14 JP JP2008516062A patent/JP2008546990A/ja active Pending
- 2006-06-14 AU AU2006257752A patent/AU2006257752B2/en not_active Ceased
- 2006-06-14 WO PCT/AT2006/000244 patent/WO2006133473A1/de active Application Filing
- 2006-06-14 AT AT06741040T patent/ATE519182T1/de active
- 2006-06-14 CN CN2006800212475A patent/CN101238487B/zh not_active Expired - Fee Related
-
2007
- 2007-11-28 ZA ZA200710297A patent/ZA200710297B/xx unknown
- 2007-12-17 US US11/957,715 patent/US8064680B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ATE519182T1 (de) | 2011-08-15 |
| CN101238487B (zh) | 2013-04-24 |
| CN101238487A (zh) | 2008-08-06 |
| WO2006133473A1 (de) | 2006-12-21 |
| EP1891602B1 (de) | 2011-08-03 |
| AU2006257752B2 (en) | 2010-09-23 |
| AU2006257752A1 (en) | 2006-12-21 |
| US20080212868A1 (en) | 2008-09-04 |
| AT501944A1 (de) | 2006-12-15 |
| JP2008546990A (ja) | 2008-12-25 |
| ZA200710297B (en) | 2008-08-27 |
| AT501944B1 (de) | 2007-06-15 |
| EP1891602A1 (de) | 2008-02-27 |
| US8064680B2 (en) | 2011-11-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |
Effective date: 20140616 |