CA2594223A1 - Amorce pour la detection d'acide nucleique - Google Patents
Amorce pour la detection d'acide nucleique Download PDFInfo
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- CA2594223A1 CA2594223A1 CA002594223A CA2594223A CA2594223A1 CA 2594223 A1 CA2594223 A1 CA 2594223A1 CA 002594223 A CA002594223 A CA 002594223A CA 2594223 A CA2594223 A CA 2594223A CA 2594223 A1 CA2594223 A1 CA 2594223A1
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- nucleic acid
- acid molecule
- isolated nucleic
- sequence
- nucleotides
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| US64130305P | 2005-01-03 | 2005-01-03 | |
| US60/641,303 | 2005-01-03 | ||
| PCT/US2006/000175 WO2006074222A2 (fr) | 2005-01-03 | 2006-01-03 | Amorce pour la detection d'acide nucleique |
Publications (2)
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| CA2594223A1 true CA2594223A1 (fr) | 2006-07-13 |
| CA2594223C CA2594223C (fr) | 2016-03-15 |
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| CA2594223A Expired - Fee Related CA2594223C (fr) | 2005-01-03 | 2006-01-03 | Amorce pour la detection d'acide nucleique |
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| US (2) | US20060188902A1 (fr) |
| EP (1) | EP1841890B1 (fr) |
| AU (1) | AU2006204087B2 (fr) |
| CA (1) | CA2594223C (fr) |
| WO (1) | WO2006074222A2 (fr) |
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| GB0514889D0 (en) * | 2005-07-20 | 2005-08-24 | Lgc Ltd | Oligonucleotides |
| US20090068643A1 (en) * | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
| US8076067B2 (en) * | 2006-08-15 | 2011-12-13 | Genetag Technology, Inc. | Probe-antiprobe compositions and methods for DNA or RNA detection |
| EP1905842A1 (fr) * | 2006-09-26 | 2008-04-02 | DiaSorin S.p.A. | Procédé de détection d'alleles mutants combinant real time pcr et rems-pcr |
| US9260746B2 (en) * | 2007-11-21 | 2016-02-16 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Photoinduced electron transfer (PET) primer for nucleic acid amplification |
| WO2010018245A1 (fr) * | 2008-08-12 | 2010-02-18 | Biotools Biotechnological & Medical Laboratories, S.A. | Méthode de détection et/ou de quantification d'un acide nucléique substrat |
| WO2010147848A2 (fr) | 2009-06-15 | 2010-12-23 | Rd Biosciences, Inc. | Coffrets et procédés pour une amplification et une détection sélective de cibles d'acide nucléique |
| US8715939B2 (en) | 2011-10-05 | 2014-05-06 | Gen-Probe Incorporated | Compositions, methods and kits to detect adenovirus nucleic acids |
| GB201017978D0 (en) | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| AU2011319755B2 (en) * | 2010-10-27 | 2017-02-23 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
| EP2633080B1 (fr) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Procédé pour la detection des molecules cibles utilisant des sondes à nanotubes d'acide nucléique marquées de façon fluorescente |
| WO2012152698A1 (fr) * | 2011-05-06 | 2012-11-15 | Qiagen Gmbh | Procédés de séquençage, d'amplification et de détection d'acides nucléiques comprenant une amorce marquée en interne |
| CN103160575B (zh) * | 2011-12-15 | 2014-10-01 | 王荣芳 | SAA1β/β纯合子基因型在肝硬化预后诊断及肝硬化诊断中的应用 |
| AU2013352067A1 (en) * | 2012-11-28 | 2015-06-04 | Abwiz Bio, Inc. | Preparation of gene-specific templates for the use in single primer amplification |
| CA2897474A1 (fr) | 2013-01-24 | 2014-07-31 | California Institute Of Technology | Caracterisation a base de chromophore et procedes de detection |
| WO2014144578A1 (fr) * | 2013-03-15 | 2014-09-18 | The Usa, As Represented By The Secretary, Department Of Health And Human Services | Détection sélective des virus des hépatites a, b, c, d ou e ou de leurs combinaisons |
| EP3030680B1 (fr) * | 2013-08-09 | 2018-10-03 | Luminex Corporation | Discrimination à l'état fondu et multiplexage dans des dosages d'acides nucléiques |
| EP4299767A3 (fr) * | 2013-08-14 | 2024-03-27 | Gen-Probe Incorporated | Compositions et procédés de détection de l'acide nucléique du hev |
| WO2016123419A1 (fr) | 2015-01-30 | 2016-08-04 | President And Fellows Of Harvard College | Imagerie sans microscope |
| CN116949140A (zh) | 2016-02-17 | 2023-10-27 | 哈佛学院院长及董事 | 分子编程工具 |
| CN106048015A (zh) * | 2016-06-07 | 2016-10-26 | 中国疾病预防控制中心传染病预防控制所 | 限制性内切酶介导的依赖于pcr扩增的实时核酸检测技术 |
| WO2018132392A2 (fr) | 2017-01-10 | 2018-07-19 | President And Fellows Of Harvard College | Amplification de signal multiplexée |
| US11981956B2 (en) | 2018-01-26 | 2024-05-14 | President And Fellows Of Harvard College | Proximity detection methods and compositions |
| CN112111566A (zh) * | 2020-09-23 | 2020-12-22 | 迈克生物股份有限公司 | 多重核酸检测方法、组合及试剂盒 |
| EP4359520A2 (fr) * | 2021-06-22 | 2024-05-01 | Janus-I Science Inc. | Procédé d'analyse large de micro-organismes et de déterminants de résistance aux médicaments dans des échantillons provenant d'un sujet et dispositifs correspondants |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5321131A (en) * | 1990-03-08 | 1994-06-14 | Hybridon, Inc. | Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling |
| WO1992006189A1 (fr) * | 1990-09-27 | 1992-04-16 | Invitrogen Corporation | Clonage direct d'acides nucleiques amplifies par reaction en chaine de la polymerase |
| US6028190A (en) * | 1994-02-01 | 2000-02-22 | The Regents Of The University Of California | Probes labeled with energy transfer coupled dyes |
| ATE295427T1 (de) * | 1996-06-04 | 2005-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| US5866336A (en) | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| DE60045059D1 (de) * | 1999-04-20 | 2010-11-18 | Nat Inst Of Advanced Ind Scien | Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten |
| US6472153B1 (en) * | 1999-10-26 | 2002-10-29 | Epoch Biosciences, Inc. | Hybridization-triggered fluorescent detection of nucleic acids |
| US7176037B2 (en) * | 2000-07-13 | 2007-02-13 | The Scripps Research Institute | Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation |
| CA2417986C (fr) * | 2000-08-11 | 2013-11-26 | University Of Utah Research Foundation | Sondes oligonucleotidiques simples, marquees |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| US7373253B2 (en) * | 2002-02-12 | 2008-05-13 | Idaho Technology | Multi-test analysis of real-time nucleic acid amplification |
-
2006
- 2006-01-03 WO PCT/US2006/000175 patent/WO2006074222A2/fr active Application Filing
- 2006-01-03 AU AU2006204087A patent/AU2006204087B2/en not_active Ceased
- 2006-01-03 US US11/325,270 patent/US20060188902A1/en not_active Abandoned
- 2006-01-03 EP EP06733602.4A patent/EP1841890B1/fr not_active Not-in-force
- 2006-01-03 CA CA2594223A patent/CA2594223C/fr not_active Expired - Fee Related
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2008
- 2008-11-10 US US12/267,869 patent/US7709626B2/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| US20090118490A1 (en) | 2009-05-07 |
| AU2006204087A1 (en) | 2006-07-13 |
| WO2006074222A3 (fr) | 2007-12-13 |
| WO2006074222A2 (fr) | 2006-07-13 |
| AU2006204087B2 (en) | 2010-09-16 |
| US20060188902A1 (en) | 2006-08-24 |
| CA2594223C (fr) | 2016-03-15 |
| EP1841890A4 (fr) | 2010-04-07 |
| EP1841890A2 (fr) | 2007-10-10 |
| US7709626B2 (en) | 2010-05-04 |
| EP1841890B1 (fr) | 2013-08-14 |
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