CA2584131A1 - P27 ubiquitination assay and methods of use - Google Patents
P27 ubiquitination assay and methods of use Download PDFInfo
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- CA2584131A1 CA2584131A1 CA002584131A CA2584131A CA2584131A1 CA 2584131 A1 CA2584131 A1 CA 2584131A1 CA 002584131 A CA002584131 A CA 002584131A CA 2584131 A CA2584131 A CA 2584131A CA 2584131 A1 CA2584131 A1 CA 2584131A1
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- ubiquitin
- label
- compound
- ubiquitinated
- protein
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- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 238000002877 time resolved fluorescence resonance energy transfer Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000012301 transgenic model Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 108010016317 ubiquitin-aldehyde Proteins 0.000 description 1
- 201000005112 urinary bladder cancer Diseases 0.000 description 1
- 208000010570 urinary bladder carcinoma Diseases 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 108091005957 yellow fluorescent proteins Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
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- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61909204P | 2004-10-15 | 2004-10-15 | |
| US60/619,092 | 2004-10-15 | ||
| PCT/US2005/037171 WO2006044747A2 (en) | 2004-10-15 | 2005-10-17 | P27 ubiquitination assay and methods of use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2584131A1 true CA2584131A1 (en) | 2006-04-27 |
Family
ID=36203595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002584131A Abandoned CA2584131A1 (en) | 2004-10-15 | 2005-10-17 | P27 ubiquitination assay and methods of use |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20060194262A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1810008A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2008517278A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2005295517A1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2584131A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2006044747A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2087355B1 (en) * | 2006-11-27 | 2014-09-24 | Cisbio Bioassays | Method for the detection of post-translational modifications |
| MX344555B (es) * | 2007-05-08 | 2016-12-20 | Hunter Douglas Ind Switzerland | Sistema de color multivariado con aplicacion de textura. |
| US20100021941A1 (en) * | 2008-07-25 | 2010-01-28 | Progenra, Incorporated | Methods of identifying modulators of ubiquitin ligases |
| GB201007185D0 (en) * | 2010-04-29 | 2010-06-09 | Iti Scotland Ltd | Ubiquitination assay |
| AU2012239943B2 (en) * | 2011-04-07 | 2016-09-22 | The Scripps Research Institute | High-throughput screening for compounds modulating expression of cellular macromolecules |
| CN103196877B (zh) * | 2013-03-08 | 2015-06-03 | 刘小龙 | 基于均相时间分辨荧光的检测重金属含量的方法 |
| CN104155452A (zh) * | 2013-05-15 | 2014-11-19 | 复旦大学 | 一种检测细胞内蛋白质降解的方法 |
| GB201415688D0 (en) * | 2014-09-04 | 2014-10-22 | Univ Edinburgh | Method and Systems |
| ES2965761T3 (es) | 2016-08-08 | 2024-04-16 | Tech Innovation Momentum Fund Israel Limited Partnership | Sistemas bacterianos para el análisis de polipéptidos ubiquitilados |
| WO2019030759A1 (en) | 2017-08-08 | 2019-02-14 | Technology Innovation Momentum Fund (Israel) Limited Partnership | CHLORAMPHENICOL-RESISTANT TRUNCATED PROTEIN AND USES THEREOF |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995011755A1 (en) * | 1993-10-28 | 1995-05-04 | Houston Advanced Research Center | Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions |
| US5463564A (en) * | 1994-09-16 | 1995-10-31 | 3-Dimensional Pharmaceuticals, Inc. | System and method of automatically generating chemical compounds with desired properties |
| US6747128B2 (en) * | 1997-08-20 | 2004-06-08 | Gpc Biotech, Inc. | Components of ubiquitin ligase complexes, and uses related thereto |
| US6413725B1 (en) * | 1998-08-07 | 2002-07-02 | California Institute Of Technology | Biochemical assay to monitor the ubiquitin ligase activities of cullins |
| AU767507B2 (en) * | 1998-08-28 | 2003-11-13 | New York University | Novel ubiquitin ligases as therapeutic targets |
| US6740495B1 (en) * | 2000-04-03 | 2004-05-25 | Rigel Pharmaceuticals, Inc. | Ubiquitin ligase assay |
| AU2002243477B2 (en) * | 2001-01-05 | 2007-12-20 | New York University | Methods to identify compounds useful for the treatment of proliferative and differentiative disorders |
| DE60220621T2 (de) * | 2001-11-09 | 2008-03-06 | Proteologics, Inc. | Posh Nukleinsäure, Polypeptide und darauf bezogene Verfahren |
| AU2003241897A1 (en) * | 2002-05-29 | 2003-12-12 | Kyowa Hakko Kogyo Co., Ltd. | Novel ubiquitin ligase |
-
2005
- 2005-10-17 JP JP2007536976A patent/JP2008517278A/ja active Pending
- 2005-10-17 US US11/253,128 patent/US20060194262A1/en not_active Abandoned
- 2005-10-17 EP EP05815021A patent/EP1810008A4/en not_active Withdrawn
- 2005-10-17 CA CA002584131A patent/CA2584131A1/en not_active Abandoned
- 2005-10-17 AU AU2005295517A patent/AU2005295517A1/en not_active Abandoned
- 2005-10-17 WO PCT/US2005/037171 patent/WO2006044747A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1810008A2 (en) | 2007-07-25 |
| US20060194262A1 (en) | 2006-08-31 |
| WO2006044747A3 (en) | 2006-10-12 |
| EP1810008A4 (en) | 2008-09-10 |
| AU2005295517A1 (en) | 2006-04-27 |
| WO2006044747A2 (en) | 2006-04-27 |
| JP2008517278A (ja) | 2008-05-22 |
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| Date | Code | Title | Description |
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| FZDE | Discontinued | ||
| FZDE | Discontinued |
Effective date: 20111017 |