CA2576464C - Methods and compositions for increasing longevity and protein yield from a cell culture - Google Patents

Methods and compositions for increasing longevity and protein yield from a cell culture Download PDF

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Publication number
CA2576464C
CA2576464C CA2576464A CA2576464A CA2576464C CA 2576464 C CA2576464 C CA 2576464C CA 2576464 A CA2576464 A CA 2576464A CA 2576464 A CA2576464 A CA 2576464A CA 2576464 C CA2576464 C CA 2576464C
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CA
Canada
Prior art keywords
cell
cell line
cells
antibody
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2576464A
Other languages
English (en)
French (fr)
Other versions
CA2576464A1 (en
Inventor
David M. Goldenberg
Zhengxing Qu
Eva Horak
Ivan D. Horak
Chien Hsing Chang
Edmund A. Rossi
Jeng-Dar Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunomedics Inc
Original Assignee
Immunomedics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunomedics Inc filed Critical Immunomedics Inc
Publication of CA2576464A1 publication Critical patent/CA2576464A1/en
Application granted granted Critical
Publication of CA2576464C publication Critical patent/CA2576464C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4747—Apoptosis related proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2576464A 2004-07-23 2005-07-25 Methods and compositions for increasing longevity and protein yield from a cell culture Expired - Lifetime CA2576464C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US59034904P 2004-07-23 2004-07-23
US60/590,349 2004-07-23
PCT/US2005/026224 WO2007015691A2 (en) 2004-07-23 2005-07-25 Methods and compositions for increasing longevity and protein yield from a cell culture

Publications (2)

Publication Number Publication Date
CA2576464A1 CA2576464A1 (en) 2006-01-23
CA2576464C true CA2576464C (en) 2014-09-09

Family

ID=37709023

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2576464A Expired - Lifetime CA2576464C (en) 2004-07-23 2005-07-25 Methods and compositions for increasing longevity and protein yield from a cell culture

Country Status (5)

Country Link
EP (1) EP1802646B1 (enExample)
JP (2) JP5378682B2 (enExample)
CA (1) CA2576464C (enExample)
SG (1) SG163592A1 (enExample)
WO (1) WO2007015691A2 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7537930B2 (en) 2004-07-23 2009-05-26 Immunomedics, Inc. Mammalian cell lines for increasing longevity and protein yield from a cell culture
KR101794638B1 (ko) * 2008-06-10 2017-12-04 상가모 테라퓨틱스, 인코포레이티드 Ｂａｘ- 및 ｂａｋ-결함 세포주들의 제조 방법 및 조성물
JP5665065B2 (ja) * 2011-04-28 2015-02-04 国立大学法人大阪大学 リソソーム病治療用医薬組成物
US9365825B2 (en) * 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
EP2970915B1 (en) * 2013-03-15 2019-08-14 Maxcyte, Inc. Methods and compositions for generating stable transfected cells
KR20170135966A (ko) 2015-04-13 2017-12-08 맥스시티 인코포레이티드 게놈 dna를 변형시키기 위한 방법 및 조성물
GB201511196D0 (en) * 2015-06-25 2015-08-12 Cytosystems Ltd Monoclonal antibodies
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0471484A (ja) * 1990-07-11 1992-03-06 Teijin Ltd 低凝集性細胞及びその培養方法
AU7614600A (en) * 1999-09-27 2001-04-30 Genentech Inc. Methods for making recombinant proteins using apoptosis inhibitors
US7604989B2 (en) * 2001-07-10 2009-10-20 Johns Hopkins University Inhibition of apoptosis process and improvement of cell performance
WO2003040374A1 (en) * 2001-11-02 2003-05-15 Centocor, Inc. METHODS AND COMPOSITIONS FOR ENHANCED PROTEIN EXPRESSION AND/OR GROWTH OF CULTURED CELLS USING CO-TRANSCRIPTION OF A Bcl2 ENCODING NUCLEIC ACID
US6964199B2 (en) * 2001-11-02 2005-11-15 Cantocor, Inc. Methods and compositions for enhanced protein expression and/or growth of cultured cells using co-transcription of a Bcl2 encoding nucleic acid
EP1490481A4 (en) * 2001-12-14 2005-12-07 Centocor Inc CLONAL CELL LINES OF MYELOMES FOR PRODUCING PROTEINS IN A CHEMICALLY DEFINED ENVIRONMENT
US20030219871A1 (en) * 2002-03-28 2003-11-27 Boehringer Ingelheim Pharma Gmbh & Co. Kg Host cells having improved cell survival properties and methods to generate such cells
US7537930B2 (en) * 2004-07-23 2009-05-26 Immunomedics, Inc. Mammalian cell lines for increasing longevity and protein yield from a cell culture

Also Published As

Publication number Publication date
CA2576464A1 (en) 2006-01-23
SG163592A1 (en) 2010-08-30
EP1802646B1 (en) 2017-09-06
JP5378682B2 (ja) 2013-12-25
EP1802646A2 (en) 2007-07-04
EP1802646A4 (en) 2009-10-21
WO2007015691A3 (en) 2009-04-02
JP2008507300A (ja) 2008-03-13
WO2007015691A9 (en) 2007-04-19
JP2012254084A (ja) 2012-12-27
WO2007015691A2 (en) 2007-02-08

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