CA2489196C - Method and test kit for quantitative determination of polynucleotides in a mixture - Google Patents

Method and test kit for quantitative determination of polynucleotides in a mixture Download PDF

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Publication number
CA2489196C
CA2489196C CA2489196A CA2489196A CA2489196C CA 2489196 C CA2489196 C CA 2489196C CA 2489196 A CA2489196 A CA 2489196A CA 2489196 A CA2489196 A CA 2489196A CA 2489196 C CA2489196 C CA 2489196C
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CA
Canada
Prior art keywords
probes
molecules
affinity
tag
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2489196A
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English (en)
French (fr)
Other versions
CA2489196A1 (en
Inventor
Hans Soederlund
Reetta Satokari
Kari Kataja
Kristiina Takkinen
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VTT Technical Research Centre of Finland
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VTT Technical Research Centre of Finland
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Filing date
Publication date
Application filed by VTT Technical Research Centre of Finland filed Critical VTT Technical Research Centre of Finland
Publication of CA2489196A1 publication Critical patent/CA2489196A1/en
Application granted granted Critical
Publication of CA2489196C publication Critical patent/CA2489196C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2489196A 2002-07-05 2003-07-04 Method and test kit for quantitative determination of polynucleotides in a mixture Expired - Fee Related CA2489196C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI20021325A FI20021325A0 (fi) 2002-07-05 2002-07-05 Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
FI20021325 2002-07-05
PCT/FI2003/000544 WO2004005545A1 (en) 2002-07-05 2003-07-04 Method and test kit for quantitative determination of polynucleotides in a mixture

Publications (2)

Publication Number Publication Date
CA2489196A1 CA2489196A1 (en) 2004-01-15
CA2489196C true CA2489196C (en) 2013-06-11

Family

ID=8564307

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2489196A Expired - Fee Related CA2489196C (en) 2002-07-05 2003-07-04 Method and test kit for quantitative determination of polynucleotides in a mixture

Country Status (12)

Country Link
US (2) US7361461B2 (enExample)
EP (2) EP1537238B1 (enExample)
JP (1) JP4672365B2 (enExample)
AT (1) ATE384141T1 (enExample)
AU (1) AU2003242804B2 (enExample)
CA (1) CA2489196C (enExample)
DE (1) DE60318723T2 (enExample)
DK (1) DK1537238T3 (enExample)
ES (1) ES2299727T3 (enExample)
FI (1) FI20021325A0 (enExample)
PT (1) PT1537238E (enExample)
WO (1) WO2004005545A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2598508A1 (fr) * 2005-02-25 2006-08-31 Commissariat A L'energie Atomique Procede et dispositif pour separer des cibles moleculaires dans un melange complexe
EP1772522A1 (en) * 2005-10-04 2007-04-11 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Control of preservation by biomarkers
EP3561071B1 (en) 2006-02-13 2020-12-16 Fluidigm Canada Inc. Gene expression assays conducted by elemental analysis
WO2007133703A2 (en) 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US20090011944A1 (en) * 2007-02-21 2009-01-08 Valtion Teknillinen Tutkimuskekus Method and test kit for detecting nucleotide variations
FI20075124A0 (fi) * 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
WO2010114599A1 (en) * 2009-04-01 2010-10-07 Dx Terity Diagnostics Inc. Chemical ligation dependent probe amplification (clpa)
US10066257B2 (en) 2010-03-29 2018-09-04 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
US9518303B2 (en) 2011-10-25 2016-12-13 The Board Of Regents Of The University Of Texas System Methods and compositions for identifying enterovirus
WO2015191777A2 (en) 2014-06-10 2015-12-17 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
US11286518B2 (en) 2016-05-06 2022-03-29 Regents Of The University Of Minnesota Analytical standards and methods of using same

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US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
NZ211453A (en) * 1984-03-22 1989-01-06 Biotechnology Research Enterpr Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US4968602A (en) 1986-03-05 1990-11-06 Molecular Diagnostics, Inc. Solution-phase single hybridization assay for detecting polynucleotide sequences
AU619170B2 (en) * 1987-01-09 1992-01-23 Abbott Laboratories Diagnostic assays using nucleic acid probes
US5082830A (en) * 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
US5633134A (en) * 1992-10-06 1997-05-27 Ig Laboratories, Inc. Method for simultaneously detecting multiple mutations in a DNA sample
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
ATE226983T1 (de) * 1994-08-19 2002-11-15 Pe Corp Ny Gekoppeltes ampflikation- und ligationverfahren
WO1996020212A2 (en) 1994-12-28 1996-07-04 Buchardt, Dorte Peptide nucleic acid incorporating a chiral backbone
DE19516196A1 (de) * 1995-05-08 1996-11-14 Boehringer Mannheim Gmbh Verfahren zum quantitativen Nachweis von Nukleinsäuren
US6136531A (en) 1995-05-08 2000-10-24 Roche Diagnostics Gmbh Method of quantitatively detecting nucleic acids
US5714386A (en) * 1996-01-11 1998-02-03 Board Of Trustees Of The Leland Stanford Junior University Cy7-allophycocyanin conjugates for use in multiplex fluorescence detection assays
PT840804E (pt) * 1996-01-23 2000-10-31 Qiagen Genomics Inc Metodos e composicoes para a analise de moleculas de acidos nocleicos usando tecnicas de separacao por dimensoes
AU730633B2 (en) * 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
IL156002A0 (en) * 1997-02-12 2003-12-23 Eugene Y Chan Methods and products for analyzing polymers
EP1015469B2 (en) 1997-09-12 2015-11-18 Exiqon A/S Bi- and tri-cyclic nucleoside, nucleotide and oligonucleoide analogues
US6562567B2 (en) * 1998-01-27 2003-05-13 California Institute Of Technology Method of detecting a nucleic acid
ATE423101T1 (de) * 1998-08-11 2009-03-15 Siemens Healthcare Diagnostics Im nahen infrarot chemilumineszente akridinium derivate und ihre verwendung
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
AU775179B2 (en) * 1999-03-15 2004-07-22 Applied Biosystems, Llc Probe/mobility modifier complexes for multiplex nucleic acid detection
FI115139B (fi) * 2001-01-10 2005-03-15 Valtion Teknillinen Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen
AU2002344221A1 (en) * 2001-05-26 2002-12-09 Aclara Biosciences, Inc. Catalytic amplification of multiplexed assay signals
WO2003033741A1 (en) * 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
US7163790B2 (en) * 2001-11-28 2007-01-16 Bio-Rad Laboratories, Inc. Parallel polymorphism scoring by amplification and error correction
WO2004031408A1 (en) * 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005047521A2 (en) * 2003-11-10 2005-05-26 Investigen, Inc. Methods of preparing nucleic acid for detection

Also Published As

Publication number Publication date
EP1537238B1 (en) 2008-01-16
FI20021325A0 (fi) 2002-07-05
JP4672365B2 (ja) 2011-04-20
WO2004005545A1 (en) 2004-01-15
DE60318723T2 (de) 2009-01-15
EP1947200A1 (en) 2008-07-23
ATE384141T1 (de) 2008-02-15
US7361461B2 (en) 2008-04-22
AU2003242804B2 (en) 2008-06-12
DK1537238T3 (da) 2008-06-02
EP1537238A1 (en) 2005-06-08
US20080233591A1 (en) 2008-09-25
CA2489196A1 (en) 2004-01-15
US8143388B2 (en) 2012-03-27
JP2005532058A (ja) 2005-10-27
US20060035228A1 (en) 2006-02-16
ES2299727T3 (es) 2008-06-01
AU2003242804A1 (en) 2004-01-23
PT1537238E (pt) 2008-04-22
DE60318723D1 (de) 2008-03-06

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Effective date: 20220301

MKLA Lapsed

Effective date: 20200831