CA2471874A1 - Compositions et methodes d'hydroxylation d'epothilones - Google Patents

Compositions et methodes d'hydroxylation d'epothilones Download PDF

Info

Publication number
CA2471874A1
CA2471874A1 CA002471874A CA2471874A CA2471874A1 CA 2471874 A1 CA2471874 A1 CA 2471874A1 CA 002471874 A CA002471874 A CA 002471874A CA 2471874 A CA2471874 A CA 2471874A CA 2471874 A1 CA2471874 A1 CA 2471874A1
Authority
CA
Canada
Prior art keywords
epothilone
seq
nucleic acid
acid sequence
hydroxylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002471874A
Other languages
English (en)
Inventor
Jonathan David Basch
Shu-Jen Chiang
Suo-Win Liu
Akbar Nayeem
Yuhua Sun
You Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Myers Squibb Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2471874A1 publication Critical patent/CA2471874A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Communicable Diseases (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La présente invention concerne des séquences d'acide nucléiques isolées et des polypeptides codés par ces dernières qui produisent de l'épothilone B hydroxylase ainsi que des mutants et des variants de cette dernière et une ferrédoxine située en aval du gène d'épothilone B hydroxylase. Cette invention concerne également des vecteurs et des cellules contenant ces vecteurs; des méthodes de production de micro-organismes de recombinaison, des méthodes d'utilisation de ces micro-organismes de recombinaison pour produire des épothilones porteuses d'un groupe hydroxyalkyle et un analogue d'épothilone produit par un mutant de l'épothilone B hydroxylase.
CA002471874A 2001-12-26 2002-12-17 Compositions et methodes d'hydroxylation d'epothilones Abandoned CA2471874A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34427101P 2001-12-26 2001-12-26
US60/344,271 2001-12-26
PCT/US2002/040359 WO2003057830A2 (fr) 2001-12-26 2002-12-17 Compositions et methodes d'hydroxylation d'epothilones

Publications (1)

Publication Number Publication Date
CA2471874A1 true CA2471874A1 (fr) 2003-07-17

Family

ID=23349792

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002471874A Abandoned CA2471874A1 (fr) 2001-12-26 2002-12-17 Compositions et methodes d'hydroxylation d'epothilones

Country Status (6)

Country Link
EP (1) EP1465908A4 (fr)
JP (1) JP2005514031A (fr)
AU (1) AU2002357872A1 (fr)
CA (1) CA2471874A1 (fr)
TW (1) TW200301697A (fr)
WO (1) WO2003057830A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1386922B1 (fr) 1996-12-03 2012-04-11 Sloan-Kettering Institute For Cancer Research Synthèse d'épitholones, intermédiaires, analogues et leur utilisation
US6884608B2 (en) * 2001-12-26 2005-04-26 Bristol-Myers Squibb Company Compositions and methods for hydroxylating epothilones
DE60326832D1 (de) * 2002-04-12 2009-05-07 Mercian Corp Expressionssystem des aus actinomyceten stammenden cytochrom p-450 in escherichia coli
WO2018091885A1 (fr) 2016-11-15 2018-05-24 Hypha Discovery Limited Hydroxylation de substrats aliphatiques ou aromatiques ramifiés au moyen du cytochrome p450 d'amycolatopsis lurida
GB201807815D0 (en) 2018-05-14 2018-06-27 Hypha Discovery Ltd Hydroxylation techniques
GB201819209D0 (en) 2018-11-26 2019-01-09 Hypha Discovery Ltd Biocatalytic techniques

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL142938A0 (en) * 1998-12-23 2002-04-21 Bristol Myers Squibb Co Microbiological method for the preparation of an epothilone
WO2003042217A2 (fr) * 2001-11-15 2003-05-22 Kosan Biosciences, Inc. Composes d'epothilone et procedes de fabrication

Also Published As

Publication number Publication date
TW200301697A (en) 2003-07-16
WO2003057830A2 (fr) 2003-07-17
AU2002357872A1 (en) 2003-07-24
JP2005514031A (ja) 2005-05-19
EP1465908A2 (fr) 2004-10-13
EP1465908A4 (fr) 2005-11-30
WO2003057830A3 (fr) 2004-08-05

Similar Documents

Publication Publication Date Title
CA2510079A1 (fr) Compositions et methodes d'hydroxylation d'epothilones
Neubauer et al. A flavin-dependent halogenase from metagenomic analysis prefers bromination over chlorination
CN106939323A (zh) 编码类异戊烯修饰酶的多核苷酸和其使用方法
TW200846470A (en) Genetically modified host cells for increased P450 activity levels and methods of use thereof
BRPI0616883A2 (pt) ácidos nucléicos que codificam enzima modificada de citocromo p450 e métodos de uso dos mesmos
Javidpour et al. Structural and biochemical analyses of regio-and stereospecificities observed in a type II polyketide ketoreductase
Marvin et al. The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1
Walker Determination of the structures of respiratory enzyme complexes from mammalian mitochondria
WO2004078978A1 (fr) Compositions et methodes pour hydroxyler des epothilones
Prosise et al. Crystal structures of Tritrichomonas foetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism
CA2471874A1 (fr) Compositions et methodes d'hydroxylation d'epothilones
Biswas et al. A new scaffold of an old protein fold ensures binding to the bisintercalator thiocoraline
CA2132556A1 (fr) ¨9 desaturase recombinante et son gene codant
Capitani et al. Structure of the soluble domain of a membrane-anchored thioredoxin-like protein from Bradyrhizobium japonicum reveals unusual properties
Watanabe et al. Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis
DE60214869T2 (de) Cytochrome p450 2c9 kristalle, strukturen und dessen verwendung
AU6523100A (en) Crystallization and structure determination of staphylococcus aureus udp-n-acetylenolpyruvylglucosamine reductase (s. aureus murb)
US20050032119A1 (en) Crystal structure of cytochrome P450
TW200418849A (en) Compositions and methods for hydroxylating epothilones
Breksa III et al. Random mutagenesis of the zinc-binding motif of betaine-homocysteine methyltransferase reveals that Gly 214 is essential
EP1623024A2 (fr) Crystaux de cytochrome p450 2c9, leurs structures et leur utilisation
Reifler et al. Engineering and rapid purification of histidine-tagged Photosystem II from Synechocystis PCC 6803
Ding et al. Characterization of a LanC-free pathway for the formation of an ll-MeLan residue and an allo AviMeCys residue in the newly identified class V lanthipeptide triantimycins
JP2006503912A (ja) チトクロムｐ４５０３ａ４の結晶構造およびその使用
Hollands et al. Analysis of the cryptic biosynthetic gene cluster encoding the RiPP curacozole reveals a phenylalanine-specific peptide hydroxylase

Legal Events

Date Code Title Description
FZDE Dead