CA2469137C - Neutral protease (np) and product by using neutral protease for tissue dissociation as well as method for the production thereof - Google Patents

Neutral protease (np) and product by using neutral protease for tissue dissociation as well as method for the production thereof Download PDF

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Publication number
CA2469137C
CA2469137C CA2469137A CA2469137A CA2469137C CA 2469137 C CA2469137 C CA 2469137C CA 2469137 A CA2469137 A CA 2469137A CA 2469137 A CA2469137 A CA 2469137A CA 2469137 C CA2469137 C CA 2469137C
Authority
CA
Canada
Prior art keywords
collagenase
neutral protease
tissue
islet
isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2469137A
Other languages
English (en)
French (fr)
Other versions
CA2469137A1 (en
Inventor
Manfred Kurfuerst
Christian Raemsch
Nicole Raemsch-Guenther
Olaf Friedrich
Silke Huettler
Daniel Brandhorst
Thierry Berney
Pascal Bucher
Heide Brandhorst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nordmark Pharma GmbH
Original Assignee
Nordmark Arzneimittel GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nordmark Arzneimittel GmbH and Co KG filed Critical Nordmark Arzneimittel GmbH and Co KG
Publication of CA2469137A1 publication Critical patent/CA2469137A1/en
Application granted granted Critical
Publication of CA2469137C publication Critical patent/CA2469137C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C12N9/6491—Matrix metalloproteases [MMP's], e.g. interstitial collagenase (3.4.24.7); Stromelysins (3.4.24.17; 3.2.1.22); Matrilysin (3.4.24.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
CA2469137A 2003-07-09 2004-06-22 Neutral protease (np) and product by using neutral protease for tissue dissociation as well as method for the production thereof Expired - Fee Related CA2469137C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10331171A DE10331171A1 (de) 2003-07-09 2003-07-09 Neutrale Protease (NP) und Produkt unter Verwendung von neutraler Protease für Gewebedissoziation sowie Verfahren zu dessen Herstellung
DE10331171.8 2003-07-09

Publications (2)

Publication Number Publication Date
CA2469137A1 CA2469137A1 (en) 2005-01-09
CA2469137C true CA2469137C (en) 2015-11-24

Family

ID=33441697

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2469137A Expired - Fee Related CA2469137C (en) 2003-07-09 2004-06-22 Neutral protease (np) and product by using neutral protease for tissue dissociation as well as method for the production thereof

Country Status (8)

Country Link
US (2) US8440445B2 (enExample)
EP (1) EP1496112B1 (enExample)
JP (3) JP2005046136A (enExample)
CN (1) CN1626653A (enExample)
AT (1) ATE471979T1 (enExample)
BR (1) BRPI0402615B1 (enExample)
CA (1) CA2469137C (enExample)
DE (2) DE10331171A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010105820A1 (en) 2009-03-19 2010-09-23 Roche Diagnostics Gmbh Improved blends containing proteases

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4408940A1 (de) 1994-03-16 1995-10-05 Boehringer Mannheim Gmbh Rekombinante Collagenase Typ II aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden
JP3028852B2 (ja) * 1994-06-24 2000-04-04 ロシュ ダイアグノスティックス コーポレーション ヒストリチクス菌から得られるコラゲナーゼおよび２つの他のプロテアーゼの精製混合物
DE4445891A1 (de) * 1994-12-22 1996-06-27 Boehringer Mannheim Gmbh Rekombinante Proteinase aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden
US5989888A (en) 1996-01-24 1999-11-23 Roche Diagnostics Corporation Purified mixture of collagenase I, collagenase II and two other proteases
WO1998022574A2 (de) * 1996-11-19 1998-05-28 Roche Diagnostics Gmbh Rekombinante collagenase typ i aus clostridium histolyticum und ihre verwendung zur isolierung von zellen und zellverbänden
US5830741A (en) * 1996-12-06 1998-11-03 Boehringer Mannheim Corporation Composition for tissue dissociation containing collagenase I and II from clostridium histolyticum and a neutral protease
CA2447818C (en) * 2001-07-02 2011-01-04 Nordmark Arzneimittel Gmbh & Co. Kg Method for purifying an enzyme and purified enzyme produced according to this method, as well as use of the enzyme

Also Published As

Publication number Publication date
EP1496112B1 (de) 2010-06-23
EP1496112A3 (de) 2005-10-12
BRPI0402615B1 (pt) 2017-07-04
EP1496112A2 (de) 2005-01-12
DE10331171A1 (de) 2005-01-27
JP2010081941A (ja) 2010-04-15
JP2005046136A (ja) 2005-02-24
CN1626653A (zh) 2005-06-15
US20050142632A1 (en) 2005-06-30
US8765471B2 (en) 2014-07-01
ATE471979T1 (de) 2010-07-15
US20130230922A1 (en) 2013-09-05
JP2011147458A (ja) 2011-08-04
CA2469137A1 (en) 2005-01-09
BRPI0402615A (pt) 2005-06-28
DE502004011298D1 (de) 2010-08-05
US8440445B2 (en) 2013-05-14

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Effective date: 20220622