CA2453864A1 - A walk-through technique for in vitro recombination of polynucleotide sequences - Google Patents

A walk-through technique for in vitro recombination of polynucleotide sequences Download PDF

Info

Publication number
CA2453864A1
CA2453864A1 CA002453864A CA2453864A CA2453864A1 CA 2453864 A1 CA2453864 A1 CA 2453864A1 CA 002453864 A CA002453864 A CA 002453864A CA 2453864 A CA2453864 A CA 2453864A CA 2453864 A1 CA2453864 A1 CA 2453864A1
Authority
CA
Canada
Prior art keywords
dna
nucleic acid
chain
molecules
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002453864A
Other languages
English (en)
French (fr)
Inventor
Zhixin Shao
Peter Kratzsch
Rainer Schmuck
Herbert Von Der Eltz
Janet Kenklies
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2453864A1 publication Critical patent/CA2453864A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C12N9/0034—Sarcosine oxidase (1.5.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002453864A 2001-06-27 2002-06-26 A walk-through technique for in vitro recombination of polynucleotide sequences Abandoned CA2453864A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01115424.2 2001-06-27
EP01115424 2001-06-27
PCT/EP2002/007060 WO2003002736A2 (en) 2001-06-27 2002-06-26 A walk-through technique for in vitro recombination of polynucleotide sequences

Publications (1)

Publication Number Publication Date
CA2453864A1 true CA2453864A1 (en) 2003-01-09

Family

ID=8177828

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002453864A Abandoned CA2453864A1 (en) 2001-06-27 2002-06-26 A walk-through technique for in vitro recombination of polynucleotide sequences

Country Status (4)

Country Link
EP (1) EP1404825A2 (OSRAM)
JP (1) JP3967319B2 (OSRAM)
CA (1) CA2453864A1 (OSRAM)
WO (1) WO2003002736A2 (OSRAM)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011019964A1 (en) * 2009-08-12 2011-02-17 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
IL276343B2 (en) * 2018-01-29 2024-10-01 St Jude Childrens Res Hospital Inc Method for nucleic acid amplification
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination

Also Published As

Publication number Publication date
JP3967319B2 (ja) 2007-08-29
EP1404825A2 (en) 2004-04-07
WO2003002736A2 (en) 2003-01-09
JP2004533259A (ja) 2004-11-04
WO2003002736A3 (en) 2003-07-31

Similar Documents

Publication Publication Date Title
US6153410A (en) Recombination of polynucleotide sequences using random or defined primers
US7833759B2 (en) Method of increasing complementarity in a heteroduplex
JP3967319B2 (ja) ポリヌクレオチド配列のインビトロ組換えのためのウォークスルー技術
CA2182393C (en) Dna mutagenesis by random fragmentation and reassembly
JP2010535034A (ja) テイラードマルチサイトコンビナトリアルアセンブリー
AU2002321251B2 (en) Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
US6955879B2 (en) Method for generating recombinant DNA library using unidirectional single-stranded DNA fragments
US6994963B1 (en) Methods for recombinatorial nucleic acid synthesis
KR100571937B1 (ko) 돌연변이 티로신 페놀리아제 및 이의 제조방법
US20030087254A1 (en) Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
JP4116615B2 (ja) 環状突然変異および／またはキメラポリヌクレオチドを得る方法
HUP9903760A2 (hu) Polinukleotid-szekvenciák rekombinációja véletlenszerű vagy meghatározott láncindítók alkalmazásával
MXPA98009854A (es) Recombinacion de secuencias de polinucleotidos utilizando cebadores aleatorios o definidos
AU7257300A (en) Recombination of polynucleotide sequences using random or defined primers
KR20000015984A (ko) 무작위 프라이머 또는 일정 프라이머를 이용한폴리뉴클레오티드 서열의 재조합 방법
AU2002307170A1 (en) Methods for the preparation of polynucleotide librairies and identification of library members having desired characteristics
ZA200308639B (en) Methods for the preparation of polynucleotide libraries and indentification of library members having desired characteristics.

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued