CA2450215A1 - Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides - Google Patents

Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides Download PDF

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Publication number
CA2450215A1
CA2450215A1 CA002450215A CA2450215A CA2450215A1 CA 2450215 A1 CA2450215 A1 CA 2450215A1 CA 002450215 A CA002450215 A CA 002450215A CA 2450215 A CA2450215 A CA 2450215A CA 2450215 A1 CA2450215 A1 CA 2450215A1
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CA
Canada
Prior art keywords
rna polymerase
mrna
rna
sequence
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002450215A
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English (en)
Inventor
Tord Hjalt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
CuraGen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CuraGen Corp filed Critical CuraGen Corp
Publication of CA2450215A1 publication Critical patent/CA2450215A1/fr
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Nouveau procédé de marquage d'ARNc utile pour sonder des microréseaux d'oligodésoxyribonucléotides. On soumet le modèle de polynucléotide voulu à une transcription inverse pour obtenir de l'ADNc bicaténaire en utilisant des amorces aléatoires incluant un promoteur d'ARN polymérase. Cet ADNc sert ensuite de modèle pour la synthèse d'ARN marqué par transcription in vitro en présence de précurseurs marqués.
CA002450215A 2001-08-16 2002-08-16 Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides Abandoned CA2450215A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31289301P 2001-08-16 2001-08-16
US60/312,893 2001-08-16
PCT/US2002/026063 WO2003016483A2 (fr) 2001-08-16 2002-08-16 Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides

Publications (1)

Publication Number Publication Date
CA2450215A1 true CA2450215A1 (fr) 2003-02-27

Family

ID=23213477

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002450215A Abandoned CA2450215A1 (fr) 2001-08-16 2002-08-16 Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides

Country Status (4)

Country Link
EP (1) EP1451346A2 (fr)
JP (1) JP2005502340A (fr)
CA (1) CA2450215A1 (fr)
WO (1) WO2003016483A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050106591A1 (en) * 2003-08-13 2005-05-19 Affymetrix, Inc. Methods and kits for preparing nucleic acid samples
JPWO2006041159A1 (ja) * 2004-10-14 2008-08-07 学校法人日本大学 プロモーター領域を付加したプライマー
ES2301342B1 (es) * 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".
JP2011519579A (ja) * 2008-05-15 2011-07-14 トピジェン ファーマスーティカルズ インク 炎症および新生細胞増殖の治療のためのオリゴヌクレオチド

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA

Also Published As

Publication number Publication date
JP2005502340A (ja) 2005-01-27
WO2003016483A2 (fr) 2003-02-27
EP1451346A2 (fr) 2004-09-01
WO2003016483A3 (fr) 2004-06-10

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Legal Events

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