CA2450215A1 - Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides - Google Patents
Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides Download PDFInfo
- Publication number
- CA2450215A1 CA2450215A1 CA002450215A CA2450215A CA2450215A1 CA 2450215 A1 CA2450215 A1 CA 2450215A1 CA 002450215 A CA002450215 A CA 002450215A CA 2450215 A CA2450215 A CA 2450215A CA 2450215 A1 CA2450215 A1 CA 2450215A1
- Authority
- CA
- Canada
- Prior art keywords
- rna polymerase
- mrna
- rna
- sequence
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Nouveau procédé de marquage d'ARNc utile pour sonder des microréseaux d'oligodésoxyribonucléotides. On soumet le modèle de polynucléotide voulu à une transcription inverse pour obtenir de l'ADNc bicaténaire en utilisant des amorces aléatoires incluant un promoteur d'ARN polymérase. Cet ADNc sert ensuite de modèle pour la synthèse d'ARN marqué par transcription in vitro en présence de précurseurs marqués.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US31289301P | 2001-08-16 | 2001-08-16 | |
US60/312,893 | 2001-08-16 | ||
PCT/US2002/026063 WO2003016483A2 (fr) | 2001-08-16 | 2002-08-16 | Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2450215A1 true CA2450215A1 (fr) | 2003-02-27 |
Family
ID=23213477
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002450215A Abandoned CA2450215A1 (fr) | 2001-08-16 | 2002-08-16 | Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1451346A2 (fr) |
JP (1) | JP2005502340A (fr) |
CA (1) | CA2450215A1 (fr) |
WO (1) | WO2003016483A2 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050106591A1 (en) * | 2003-08-13 | 2005-05-19 | Affymetrix, Inc. | Methods and kits for preparing nucleic acid samples |
JPWO2006041159A1 (ja) * | 2004-10-14 | 2008-08-07 | 学校法人日本大学 | プロモーター領域を付加したプライマー |
ES2301342B1 (es) * | 2006-03-14 | 2009-05-01 | Oryzon Genomics, S.A. | "metodo de analisis de acidos nucleicos". |
JP2011519579A (ja) * | 2008-05-15 | 2011-07-14 | トピジェン ファーマスーティカルズ インク | 炎症および新生細胞増殖の治療のためのオリゴヌクレオチド |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030104432A1 (en) * | 2001-07-27 | 2003-06-05 | The Regents Of The University Of California | Methods of amplifying sense strand RNA |
-
2002
- 2002-08-16 CA CA002450215A patent/CA2450215A1/fr not_active Abandoned
- 2002-08-16 JP JP2003521792A patent/JP2005502340A/ja active Pending
- 2002-08-16 EP EP02757157A patent/EP1451346A2/fr not_active Withdrawn
- 2002-08-16 WO PCT/US2002/026063 patent/WO2003016483A2/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
JP2005502340A (ja) | 2005-01-27 |
WO2003016483A2 (fr) | 2003-02-27 |
EP1451346A2 (fr) | 2004-09-01 |
WO2003016483A3 (fr) | 2004-06-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |